Technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and application of technology

A genotoxicity, cell technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem that the experimental plan needs to be established and perfected.

Inactive Publication Date: 2015-08-12
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

TK6 human lymphoblasts are a stably constructed cell line. Its source is human lymphoblastoid cells, which can be stably subcultured. TK6 cells have been widely used in micronucleus experiments, and the results show that the results are stable and sensitive. However, specific experimental protocols for genotoxicity testing in environmental waters need to be established and improved

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  • Technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and application of technology
  • Technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and application of technology
  • Technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and application of technology

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Embodiment Construction

[0046] The present invention is an implementation method for detecting genotoxicity of micronucleus experiments on environmental water samples. This method has been applied in the comprehensive monitoring of environmental health in the Huaihe River Basin, and the genotoxicity of some surface water and groundwater has been detected, and its practical application has been explored and analyzed. Complete. The specific application conditions are as follows:

[0047] 12 groundwater samples, 1 centralized water supply sample, and 3 surface water samples were collected from 3 villages in some parts of City F, and the collected samples were pretreated, cell cultured, micro Test procedures such as nuclear rate test and micronucleus rate counting. See Table 1 for specific sampling point information.

[0048] Table 1 Water sample collection information

[0049]

[0050] 1 Water sample pretreatment

[0051] At least 10L of water sample was collected for each sample, and after being...

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Abstract

The invention provides a technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and an application of the technology and belongs to the fields of environmental science and health risk assessment. The technology for detecting the TK6 cell chromosome damage genetic toxicity caused by the environmental water comprises a pre-treatment process on an environmental water sample, a cell culture and contamination method, a micronucleus slice preparation process and a result analysis method. The provided micronucleus experiment method can be directly applied to monitoring of the genetic toxicity of the environmental water, and important genetic toxicity effect information is provided for water pollution health risk management.

Description

technical field [0001] The invention relates to a high-throughput rapid detection method for genotoxicity of TK6 cell chromosome damage caused by environmental water, which belongs to the field of environmental science and health risk assessment, and is an experimental method for detecting genotoxicity of water environment samples. The detection technology and application of nuclear experiment to detect the genotoxicity of eukaryotic cell chromosome damage caused by environmental water. Background technique [0002] The comprehensive genotoxicity monitoring of environmental water plays an important role in the comprehensive monitoring of environmental health, which can clarify the harm of environmental pollution to the health of the population. Environmental monitoring often focuses on the detection of physical and chemical indicators, such as comprehensive indicators such as COD and the content of specific pollutants such as heavy metals, and then judges the status of envir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 王先良郭辰吕占禄钱岩吴家兵梁豹张金良
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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