3243results about How to "Convenient research" patented technology

Methods are provided for the genetic control of pathogen infestation in host organisms such as plants, vertebrate animals and fungi. Such methods utilize the host as a delivery system for the delivery of genetic agents, preferably in the form of RNA molecules, to a pathogen, which agents cause directly or indirectly an impairment in the ability of the pathogen to maintain itself, grow or otherwise infest a host plant, vertebrate animal or fungus. Also provided are DNA constructs and novel nematode nucleotide sequences for use in same, that facilitate pathogen resistance when expressed in a genetically-modified host. Such constructs direct the expression of RNA molecules substantially homologous and/or complementary to an RNA molecule encoded by a nucleotide sequence within the genome of a pathogen and/or of the cells of a host to effect down-regulation of the nucleotide sequence. Particular hosts contemplated are plants, such as pineapple plants, and particular pathogens are nematodes.

A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.

The present invention provides a system and method for using key-value pairing to identify uniquely a communication device on a mobile network. In some embodiments a user of the device visits the mobile network site of a publisher. The publisher sends header information received from the device to an inventoried content delivery service (ICDS). The ICDS analyzes the header information associated with the device and forwarded by the publisher. The ICDS locates a key-value pair in the header data that can be uniquely associated with the device, and stores the exact value of the key-value pair in a database. The ICDS then may use that value as an index for accumulating information accompanying the value. Thus, the ICDS learns about the actions taken on the mobile network using the device, and can use this information in a variety of ways.

Apparatus, method and computer products are provided for remote network access and management of mobile terminals. The mobile terminal executes a web server application that allows remote network devices to access the mobile terminal. In addition, the mobile device executes a content engine that provides the ability to access databases, services and devices associated with the mobile terminal. The invention provides greater flexibility in accessing and managing the data stored on the mobile terminal and provides for remote control of the services and devices associated with the mobile terminal.

The invention relates to a wireless-receiving system for detecting microelectronic mechanical microwave frequency and a preparation method thereof. The wireless-receiving system for detecting the microelectronic mechanical microwave frequency has quite simple structure, large measurement magnitude range, no direct-current power consumption and easy integration. In the system for detecting the microelectronic mechanical microwave frequency, gallium arsenide is used as a substrate, wherein a microwave antenna (A), a one-three power splitter (B), a coplanar waveguide transmission line (C), a two-in-one power combiner (D), an MEMS cantilever capacitive microwave power sensor (E) and an MEMS thermoelectric microwave power sensor (F) are designed on the substrate; and then a phase difference between a signal 3 and a signal 2 after the signal 3 passes through the coplanar waveguide transmission line with the length of lambda/2 can be determined according to a law of cosines. Because the phase difference corresponds to the frequency of the signal, the frequency of the signal can be measured.

A method, system, and computer readable medium executable on a computer for at least one of filtering, segmenting, compressing and classifying an ECG or similar signal includes the steps of fitting a nonlinear signal model to the signal using an optimization algorithm, such as nonlinear least squares, and determining features in the nonlinear signal model.

this invention relates to power in direct-current circuits. The invention takes gallium arsenide as foundation (1). On the foundation has power divider, power synthesizer, 90deg phaser, solid beam structure. Power divider and power synthesizer composed by port one (7), port two(8), port three(8), dissymmetry coplane band line(10), nitride tantalum electric resistance. Solid beam structure includes signal input port(16), pier(5), solid beam(13), pedestal structure(6), sensing electrode(4), sensing electrode leader(12), capacitance detecting port(15), air bridge(14). There are nitriding silicon medium layer on the transmission line, pedestal structure and sensing electrode under solid beam, and sensing electrode leader under Air Bridge.

The invention discloses a psychological stress assessment method based on multi-physiological-parameter integration. The method includes: designing a reasonable stimulation program, acquiring four types of electrophysiological signals, namely electrocardiogram signals, electromyographic signals, pulse wave signals and electroencephalogram signals from people suffering psychological stress; extracting affective features of the four types of electrophysiological signals; subjecting the extracted features to feature selection by means of Relief algorithm, genetic algorithm optimization and the like; acquiring related integration functions on the basis of basic probability assignment mass. According to the method, multi-parameter signals are subjected to acquisition, preprocessed, feature selection and psychological stress affective recognition and are integrated; compared to single-parameter classified recognition or multi-parameter data-level or feature-level integration, the method allows data information to be more fully utilized and psychological stress emotions to be more accurately recognized.

The invention discloses a microwave power detection system based on parallel-connected MEMS (micro-electromechanical system) cantilever beams and a preparation method of the microwave power detection system. The method is used for on-line measurement, electrostatic force can be generated between each MEMS cantilever beam and a transmission line in a center signal line transmission process, the cantilever beams are pulled downwards, and an interval between each cantilever beam and a corresponding test electrode becomes smaller, so that capacitance values are changed and microwave power in one-to-one correspondence with the capacitance values is obtained by measuring the changed capacitance values. The microwave power detection system based on the parallel-connected MEMS cantilever beams comprises a gallium arsenide substrate, wherein a plane-waveguide center signal line (A), a parallel-connected MEMS cantilever beam structure (B) and a capacitance type microwave power sensor (C) are arranged on the substrate. When a microwave signal is transmitted on the plane-waveguide center signal line, the two parallel-connected MEMS cantilever beams generate displacement under the action of the electrostatic force, and the to-be-measured power is detected by the capacitance type microwave power sensor.

Stem cell reprogramming genes cloned into a single sustained expression-type Sendai viral vector are shown to reprogram differentiated somatic cells into induced pluripotent stem (iPS) cells without integration of vector sequences into the host cell's genome. The genes are transduced into normal differentiated somatic cells via infection with recombinant Sendai virus. After expression of the reprogramming genes and subsequent induction of pluripotency, the vector genome RNA including the reprogramming genes is removed from the cell to establish an iPS cell that is genetically identical to the parent somatic differentiated cell thus reducing the risk of tumorigenic transformation caused by random integration of vector sequences into the host genome. The method promises to provide safe, autologous iPS cells that can be used for human cell replacement and regeneration therapeutic applications.

The method thereof comprises: setting up a first clamped-end beam structure, a second clamped-end beam structure, a third clamped-end beam, a fourth clamped-end beam structure, a first power distributor, a second power distributor, a first power a combiner, a second power combiner, a first 45 deg shifter, and a second 45 deg shifter on the substrate.

The invention relates to a pore network model (PNM)-based bionic bone scaffold constructing method. The method comprises the following steps of: acquiring a cross section image of microscopic three-dimensional micropore structural information and three-dimensional space position density information of a human bone by a micro computed tomography (Micro-CT) technology; performing threshold value processing to acquire binarized image data; extracting a spongy bone part, and measuring by using Mimics software to acquire porosity, penetration rate, aperture and the like; programming PNM bone scaffold parameters according to a PNM principle by using the acquired bone overall dimension data and internal size data; acquiring a generating program of the bone scaffold by using a programming tool C++ and OPENGRIP language programming; generating a three-dimensional model of the PNM bionic bone scaffold by using a Unigraphics (UG) secondary development platform; and finally leading the PNM bionic bone scaffold into the Mimics software to verify the parameters, such as the aperture, the penetration rate and the like of the PNM bionic bone scaffold. The bone scaffold well imitates a natural bone, and has high performance similar to that of the natural bone; and a good porous structure and the high penetration rate are favorable for differentiation and flowing of bone derived cells.

Markers useful for the identification, characterization and, optionally, the enrichment or isolation of tumorigenic cells or cell subpopulations are disclosed.

The invention belongs to the technical field of animal virology and genetic engineering, relating to pseudorabies virus which lacks parts of virulence gene of min strain A of pseudorabies virus, and the process for preparing vaccine with the virus. The DNA recombinant technology is employed to artificially remove all or parts of albumen code area of a plurality of genes of TK, gE, gI, 11K, and 28k which are unnecessary genes and duplicate virus of viruses and influence the virulence in the pseudorabies virus, thereby lowering the virulence of the pseudorabies virus, achieving attenuated vaccine strains SA215 of which the attenuation genetic engineering is lack, preparing vaccine with SA215 virus, and immunizing animals to effectively prevent and control the occurrence and prevalence of the pseudorabies.

The invention provides a virtuality-reality integrated three-dimensional display method, which is used for displaying virtual network elements and real entity objects in a geographic information system (GIS) to form a composite space. The display method includes the following steps: (1) the network elements are GISized; (2) the composite space is three-dimensionally visualized. The invention also provides another display method and a display system. The invention is used for virtually and omnidirectionally displaying virtual network elements in a real space and accurately obtaining the spatial positions and attribute information of the virtual network elements for the research of the distribution law and movement law of the virtual network elements.

The invention discloses a 2-(2-(diphenyl phosphine) phenyl) oxazoline nickel halogenide series complex. Said invention provides the structure formula of said complex and its preparation method. Said invention also provides catalyst composition containing said complex and its application in ethylene oligopolymerization. Said catalyst composition has higher ethylene oligopolymerization activity, and the obtained oligomer takes ethylene dimmer and trimer as main components.

A social networking website system for an individual investor to join as a member, to meet online with other investors and discuss investing. The social network facilitates finding and communicating among like-minded individuals by providing the investor with a plurality of searching tools for searching a member profile page, a portfolio, and provides online communication tools such as a chat room, a forum and video posting. The member easily researches important information regarding stocks, commodities, bonds, foreign currency, futures, and other types of financial instruments by entering a ticker symbol that becomes a prefix key linking to an online data synopsis about the investment. The investor social networking system also provides a member a mechanism for placing orders to buy or sell financial instruments through the website.

The present invention provides one kind of turtle-shaped bionic underwater robot, which includes one turtle-shaped casing, one sensing and measuring unit in the head and shoulder part, one control and drive unit in the chest, one power source unit in the abdomen, one communication system unit in the tail, one front limb moving unit and one back limb moving unit. The sensing and measuring unit consists of one sonar detector, one underwater CCD camera, and multiple light sources; the control and drive unit consists of one executing controller and one coordinating controller; the power source unit includes one motor driving power module and controller power module; and the communication system unit consists of one communication system module and one external antenna. The present invention has the advantages of high flexibility, low power consumption, one noise, great traveling range, etc.

The invention provides an enrichment and analysis method for circulating tumor cells. The method mainly comprises the following steps: subjecting a biological fluid sample to red blood cell lysis treatment; adding immune beads specifically bonding with white cells, red cells and/or platelets for incubation; and filtering cell suspension by using a filter membrane and enriching circulating tumor cells. According to the invention, through integration and optimization of CTCs enrichment methods such as red blood cell lysis treatment, an immune bead method and membrane filtration, the enrichment effect of the circulating tumor cells is greatly improved; with the method, the removal rate of leukocytes is greater than 99.9%, and the enrichment rate of tumor cells is greater than 80%. Meanwhile, the invention also provides a kit using the enrichment method for the circulating tumor cells and discloses a device for enrichment of the circulating tumor cells. The invention also provides an analysis method for the enriched circulating tumor cells. With the analysis method, analysis of dynamic changes like the amount of tumor cells, gene mutation and gene expression profiles can be realized.

The invention monitors display (error) messages from software programs presented to users and looks for items of interest. When detected, the text of the message is collected along with source of the message, and other details. This data is checked against a database of Messages, either locally, or networked to a remote database. If the issue has not been previously detected it is added to the database. If the message had been detected previously, counters recording frequency of occurrence. Then an option to see additional information is provided. If selected the database returns all the information it has on this specific issue. Also, additionally created content that normally does not exist in the message such as which application and vendor issued the message, frequency of occurrence, and vendor contact information. Additional content such as a detailed description and solutions that were manually created is included in the results to the user. The user can perform additional research and the collected information can be emailed to another user or company.

The invention relates to a three-dimensional printing method and device for tissue/organ chip integrated manufacturing. The three-dimensional printing method comprises the following steps: (1), designing the three-dimensional structural drawing of a three-dimensional chip, and converting the three-dimensional structural drawing into a slice layer graphic file format; (2), starting a three-dimensional printing device, and absorbing printing ink into all spraying heads; leading in slice layer graphic files; (3),respectively printing a main body material printing ink, a sacrificial material printing ink and different cell printing ink into pre-designed positions of a bottom plate system through the three-dimensional printing device; (4), repeating the step (3) and accomplish printing of a three-dimensional chip structure layer by layer and in an accumulating manner until the printing to a slice layer graphic file is finished; (5), heating or refrigerating the three-dimensional chip integrally printed, so as to enable a channel sacrificial material to be changed into a sol state; (6), using a pipette to absorb the channel sacrificial material in the sol state, removing the channel sacrificial material, and forming an integrated three-dimensional chip structure; (7), carrying out crosslinking on the cell printing ink material not molten, and carrying out culture medium perfusion.

The invention relates to a micro electric mechanical microwave frequency checker, and a relative production, with simple structure, small amplitude of measuring signal, non direct-current power consumption, and easy integration. The checker uses gallium arsenide as a liner (1) arranged with a power distributor (a), a power synthesizer (b), a coplane waveguide line (e), and a fixing beam structure (c), wherein the object signal via the power distributor (a) generates two signals, to form an object signal and another object signal (Us1-Us2), the object signal Us1 is connected with the third port of the power synthesizer (b), the object signal Us2 via a section of a CPW line is connected to the second port of the synthesizer (b) in which port to obtain a first signal via the fixing beam structure (c) to obtain the phase shift phi of the microwave signal on the line with fixed length.

The invention relates to a microelectronic mechanical microwave frequency detector and a preparation method thereof. The microelectronic mechanical microwave frequency detector has simple structure, can measure unknown microwave signals, has large amplitude range of the measurement signals, can avoid consuming direct current power, and is easy to be integrated. The microelectronic mechanical microwave frequency detector adopts a gallium arsenide substrate provided with a 1-to-3 power distributor (a), a coplanar wave guide transmission line (b), a 2-to-1 power distributor (c), a fixing beam structure (d) and a thermocouple structure (e), thereby forming the microelectronic mechanical microwave frequency detector.

The invention discloses a wireless multimedia sensor network-based system and method for monitoring the information of a livestock and poultry facility welfare breeding environment. At least one image acquisition node and at least one routing node are arranged in each livestock and poultry housing, at least one sensing terminal node, the sensing terminal node and the image acquisition node acquire various parameters and image signals of the livestock and poultry housing to be monitored, the parameters and the image signals are transmitted to a network relay station via the routing node, the network relay station is wirelessly connected with a data server in a remote monitoring center via a network, the server simply processes the information after receiving the information, the information is displayed in the monitor center and uploaded to the network in real time, and if temperature and other parameters exceed a predetermined range, alarm is triggered and the result is sent to onsite breeding technicians in the form of a short message. The system can acquire the environment information of the livestock and poultry housings and the image information of livestock and poultry behaviors in real time, which is favorable for the study on the relation between the environment information of the livestock and poultry housings and the comfort of livestock and poultry and for the study on the relation between livestock and poultry behaviors and the comfort of livestock and poultry.

The invention discloses an experimental simulation device and a method for exploiting natural gas hydrate. The experimental simulation device comprises a stable pressure gas/liquid supply module, a reactor, a control module, a data measuring module and a data acquisition and processing module. The method comprises the following steps: discharging unreacted gases in an isobaric manner after the hydrate is generated, subsequently compressing the hydrate into a dense hydrate piece, introducing a CO2 gas from one side of the hydrate piece, reducing the pressure on the other side of the hydrate piece, and thus achieving combination of two natural gas hydrate exploitation methods of CO2 exchange and pressure reduction. The experimental simulation device overcomes the defects that the efficiency is low and the speed is low in natural gas hydrate exploitation in the CO2 exchange mode, overcomes the difficulty that geological disaster can be caused in natural gas hydrate exploitation in the pressure reduction mode, improves the efficacy in natural gas hydrate exploitation, and moreover is simple in structure and low in cost.

The invention discloses an image identification method of stirring friction welding arc stripe interval. The image identification method is characterized by mainly comprising the steps of: obtaining image, carrying out binarization processing on the image, carrying out post processing on the binary image and detecting and calculating the arc stripe interval, firstly, obtaining a microscopic picture of a stirring friction welding arc stripe tool; and then, performing gray scale, wave filtering and binarization processing on the microscopic picture of the arc stripe tool; removing the isolated points, the interior stripe detects and the incomplete edge stripes of the binary image; and finally, determining the arc stripe interval calculation range, calculating the arc stripe interval and processing the data. The image identification method of stirring friction welding arc stripe distance disclosed by the invention greatly enriches the obtaining method of arc stripe parameters and provides certain foundation for research of the arc stripe parameters in the stirring friction welding.