The invention relates to a three-dimensional printing method and device for tissue / organ
chip integrated manufacturing. The three-dimensional printing method comprises the following steps: (1), designing the three-dimensional
structural drawing of a three-dimensional
chip, and converting the three-dimensional
structural drawing into a slice layer graphic
file format; (2), starting a three-dimensional printing device, and absorbing
printing ink into all spraying heads; leading in slice layer graphic files; (3),respectively printing a main body material
printing ink, a sacrificial material
printing ink and different
cell printing ink into pre-designed positions of a bottom plate
system through the three-dimensional printing device; (4), repeating the step (3) and accomplish printing of a three-dimensional
chip structure layer by layer and in an accumulating manner until the printing to a slice layer graphic file is finished; (5), heating or refrigerating the three-dimensional chip integrally printed, so as to enable a channel sacrificial material to be changed into a
sol state; (6), using a
pipette to absorb the channel sacrificial material in the
sol state, removing the channel sacrificial material, and forming an integrated three-dimensional chip structure; (7), carrying out crosslinking on the
cell printing ink material not molten, and carrying out culture medium
perfusion.