11 results about "Enrichment methods" patented technology

This application relates to an in-situ self-aeration enrichment device for microorganisms in the vadose zone of contaminated soil, comprising an in-situ enrichment module, a filling matrix, and a composite nutrient additive. The in-situ enrichment module consists of a cuboid, a smooth frustum, and a long cylindrical air-guiding channel, which are integrally formed from bottom to top. The filling matrix is ​​placed into the enrichment module through a fine-mesh mesh bag. The composite nutrient additive is injected into the enrichment module through the long cylindrical air-guiding channel. This enrichment device improves the enrichment efficiency of uncultured microorganisms in the vadose zone of organically contaminated sites through the combined action of the in-situ enrichment module, the filling matrix, and the composite nutrient additive.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a preparation method of a directional pore channel integral material and a method for constructing an enrichment device and establishing a rapid phosphopeptide enrichment method by using the directional pore channel integral material. The preparation method comprises the following steps: preparing a CS-PC integral material with directional pore channels from chitosan, zirconium amino phthalocyanine and polyethylene glycol diglycidyl ether through a directional freezing method and a chemical crosslinking method, and then modifying the CS-PC integral material with 5 '-phosphopyridoxal and zirconium oxychloride to obtain the CS-PC PLP integral material. The CS-PC-PLP integral material prepared by the invention has anisotropy and a directional pore channel structure, is convenient to fix in a pipette tip and is combined with a row pipettor for rapid enrichment; the CS-PC coated PLP integral material has the advantages of green and cheap raw materials and strong reusability.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

A computer system, computer program product, and computer-implemented method for improving performance of a dialog system employing an automated virtual dialog agent. The method involves receiving a natural language request and generating a corresponding response with an automated virtual agent, automatically identifying and resolving a corresponding knowledge gap between the request and the response, and refining the automated virtual agent with the resolved knowledge gap.

The application discloses a metal wastewater separation and enrichment method and equipment based on intelligent pH value regulation, relates to the field of industrial wastewater treatment, and comprises the following steps: collecting wastewater monitoring data in a current period through an online monitoring network and sending the wastewater monitoring data to a PLC automatic control system; the PLC automatic control system makes intelligent regulation and control decisions according to the wastewater monitoring data in the current period and generates control instructions. The control instructions drive the reagent feeding system and the automatic process device to operate cooperatively, and finally ensure that the heavy metals are efficiently precipitated and separated. The application realizes precise reagent feeding and process cooperation for metal wastewater separation and enrichment, improves the control precision, processing efficiency and stability of metal separation, can realize full-process automatic control, improves the metal recovery purity and processing efficiency, reduces reagent consumption, and has self-optimization capability.

Provided is a gene target region enrichment method and a kit. The method comprises (1) amplifying fragmented DNA comprising a target region by means of a specific probe so as to obtain a captured-extension product, wherein the specific probe comprises a sequence complementary to the target region of the fragmented DNA, and the 3′ terminal nucleotide of the specific probe is modified to prevent a ligation reaction at the 3′ terminal of the specific probe; and (2) linking the 3′ terminal of the captured-extension product obtained in step (1) to linker DNA to obtain a ligation product.

The application provides a Kr enrichment method and system based on carbon molecular sieve gradient adsorption, a terminal and a storage medium, relates to the technical field of Kr enrichment, and comprises the following steps: obtaining a system trigger signal; controlling a preset cooling device to cool a preset primary adsorption column based on the system trigger signal, and obtaining a cooling completion signal; controlling a preset ventilation device to introduce a preset sample gas into the column based on the cooling completion signal, and obtaining a ventilation trigger signal; obtaining a transient flow value, a krypton inlet concentration and a krypton outlet concentration based on the ventilation trigger signal; analyzing the transient flow value and a preset standard flow value to determine a pore size detection result; analyzing the krypton inlet concentration, the krypton outlet concentration and the pore size detection result to determine actual adsorption parameters; and controlling a preset primary separation module to enrich krypton according to the actual adsorption parameters. The application has the effect of ensuring efficient enrichment of krypton by carbon molecular sieve.

A method for isolating circulating tumor cells (CTCs) from a sample comprising or suspected of comprising CTCs, the method comprising: incubating the sample with immune microparticles, thereby forming cell and microparticle aggregates (CPAs) in an incubation solution wherein the CPAs have an average size greater than 10 m; and isolating the CPA using a size-based isolation method to form an isolated CTC wherein the immune microparticle comprises one or more first antibodies conjugated to the microparticle wherein the one or more first antibodies specifically bind to one or more markers of CTC.

The invention provides a Kr enrichment method and system based on carbon molecular sieve gradient adsorption, a terminal and a storage medium, and relates to the technical field of Kr enrichment. Controlling a preset cooling device to cool a preset primary adsorption column based on the system trigger signal, and obtaining a cooling completion signal; controlling a preset ventilation device to introduce preset sample gas into the column based on the cooling completion signal, and obtaining a ventilation trigger signal; acquiring an instantaneous flow value, krypton inlet concentration and krypton outlet concentration based on the ventilation trigger signal; analyzing the instantaneous flow value and a preset standard flow value to determine an aperture detection result; analyzing krypton inlet concentration, krypton outlet concentration and aperture detection results to determine actual adsorption parameters; and controlling a preset primary separation module to enrich krypton according to the actual adsorption parameters. The method has the effect of ensuring efficient enrichment of krypton by the carbon molecular sieve.

A method is provided for enriching extracellular DNA from a biological sample comprising extracellular DNA and extracellular vesicles, wherein the method comprises: (a) preparing a binding mixture comprising—the biological sample, —a solid phase comprising anion exchange groups, —an acidic binding buffer comprising a buffering agent, and binding extracellular DNA to the solid phase comprising anion exchange groups; (b) separating the solid phase with the bound extracellular DNA from the remaining binding mixture, wherein the remaining binding mixture comprises extracellular vesicles. The method may furthermore comprise processing the remaining binding mixture to enrich one or more biological targets of interest therefrom, wherein processing may comprise (c) enriching as biological targets extracellular vesicles and / or extracellular RNA from the remaining binding mixture.