26 results about "Enrichment methods" patented technology

The invention discloses a micro-fluidic chip-based trace tumor nucleic acid low-damage enrichment method and system, and the method comprises the steps: constructing a virtual flow channel network digital twin model of a multi-stage micro-fluidic chip, the virtual flow channel network digital twin model comprises enrichment units connected in series, each enrichment unit is composed of a tapered convergence section, a periodic bending section and an aptamer modified amplification section; and executing real-time simulation of fluid dynamics based on the digital twinborn model, generating a dynamic pressure control instruction set, and adjusting the flow velocity distribution of the biological sample in the flow channel through a negative pressure system driven by a fuzzy PID (Proportion Integration Differentiation) controller. According to the invention, through digital twin dynamic reconstruction flow channel and multi-modal fusion regulation, shear stress accumulation is inhibited to maintain nucleic acid integrity, impurity adsorption is reduced based on dynamic balance of probe density and binding saturation, and multi-field collaborative optimization is realized.

This application relates to an in-situ self-aeration enrichment device for microorganisms in the vadose zone of contaminated soil, comprising an in-situ enrichment module, a filling matrix, and a composite nutrient additive. The in-situ enrichment module consists of a cuboid, a smooth frustum, and a long cylindrical air-guiding channel, which are integrally formed from bottom to top. The filling matrix is ​​placed into the enrichment module through a fine-mesh mesh bag. The composite nutrient additive is injected into the enrichment module through the long cylindrical air-guiding channel. This enrichment device improves the enrichment efficiency of uncultured microorganisms in the vadose zone of organically contaminated sites through the combined action of the in-situ enrichment module, the filling matrix, and the composite nutrient additive.

The invention discloses a targeted enrichment method for proteome. According to the cell nucleus targeted RNA binding protein enrichment reagent, one end is provided with a furocoumarin group, the other end is provided with a cell nucleus targeted group, and the other end is provided with biotin; the enrichment or identification of the RNA binding protein in the cell nucleus can be carried out. The method for enriching the nucleus-targeted RNPs by adopting the nucleus-targeted RNA binding protein enrichment reagent comprises the following steps: firstly, adding the nucleus-targeted RNA binding protein enrichment reagent into cells for co-incubation, then, irradiating a cell incubation system by adopting ultraviolet light with the wavelength of 254nm, and then, irradiating the cell incubation system by adopting ultraviolet light with the wavelength of 365nm, then carrying out cracking to obtain a cracking solution; incubating the lysis solution and streptavidin magnetic beads, and finally collecting the magnetic beads enriched with the cell nucleus RNPs, so that the cell nucleus targeted RNPs are enriched. According to the method, the cell nucleus positioning of the reagent can be efficiently realized, the RBPs enrichment selectivity and the negative electrode efficiency are improved, and the varieties of RNPs obtained through enrichment are more comprehensive.

To provide an oxygen isotope concentrator where the reduction of an oxygen treatment amount can be performed by simplifying device configuration.SOLUTION: An oxygen isotope concentrator 1 comprises: a plurality of device groups having an ozone generation device 11, a carbon tetrafluoride feeder 17, an oxygen separator 12, an ozone photolysis device 13, a collector 14 and a concentrator 15; one or more oxygen supply passages 16 introducing oxygen separated at the concentrator 15 of the kth group (1≤k≤(n-1)) to the ozone generation device 11 of the (k+1)th group; and one or more mixed gas return passages 23' by which a mixed gas of ozone separated at the concentrator 15 of any jth group (2≤j≤n) and carbon tetrafluoride is introduced to any one of the first group to the (j-1) group.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of analytical chemistry, and particularly relates to a preparation method of a directional pore channel integral material and a method for constructing an enrichment device and establishing a rapid phosphopeptide enrichment method by using the directional pore channel integral material. The preparation method comprises the following steps: preparing a CS-PC integral material with directional pore channels from chitosan, zirconium amino phthalocyanine and polyethylene glycol diglycidyl ether through a directional freezing method and a chemical crosslinking method, and then modifying the CS-PC integral material with 5 '-phosphopyridoxal and zirconium oxychloride to obtain the CS-PC PLP integral material. The CS-PC-PLP integral material prepared by the invention has anisotropy and a directional pore channel structure, is convenient to fix in a pipette tip and is combined with a row pipettor for rapid enrichment; the CS-PC coated PLP integral material has the advantages of green and cheap raw materials and strong reusability.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

The invention provides a group of enrichment primers, a pathogenic microorganism enrichment kit, a pathogenic microorganism enrichment method and related application. The enrichment primer provided by the invention is as shown in a table 1, the amplification fragment of the enrichment primer and the enrichment kit provided by the invention on target pathogenic microorganisms is about 1kb, and compared with a conventional short amplification fragment, the detection accuracy of a long sequence amplified by the enrichment primer and the enrichment kit is higher. According to the enrichment method disclosed by the invention, the read length of a sequencing fragment is increased, and the capability of precise typing of microorganisms and drug-resistant / virulence genes is further improved. The enrichment primer, the kit and the enrichment method can be combined with a single molecule sequencing technology, the sequencing period is shortened, sample sequencing can be completed within one hour, the sample detection period is greatly shortened, and the enrichment primer, the kit and the enrichment method are more suitable for the field of clinical pathogen rapid detection.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

The invention relates to an enrichment method and application of antimony oxidizing microorganisms, and belongs to the technical field of microorganisms. According to the enrichment method, on the premise that active cells are reserved, the antimony oxidation function microbiota in the solid sample microbe seed bank is efficiently enriched, the influence of a matrix on the enrichment result and the activity of the antimony oxidation microbiota can be effectively reduced, and the enrichment method has the advantages of being convenient, efficient and small in error and has a certain pollution treatment application prospect.

The invention discloses a nucleic acid reverse enrichment method which comprises the following steps: extracting and purifying nucleic acid in a sample to obtain a nucleic acid sample, and if the nucleic acid is RNA (Ribonucleic Acid), carrying out reverse transcription on the purified RNA to obtain cDNA (Complementary Deoxyribose Nucleic Acid); the method comprises the following steps: carrying out nucleic acid fragmentation, terminal repair and linker connection on a nucleic acid sample, inactivating or removing ligase, adding an RNA probe specifically bound with non-target DNA, and carrying out hybridization annealing to obtain a mixture; adding enzyme for cutting a DNA chain in the DNA-RNA hybrid compound into the mixture for incubation and magnetic bead purification; carrying out PCR amplification and magnetic bead purification on the purified nucleic acid; according to the method, non-target nucleic acid molecules are cut off by using specific endonuclease, the non-target nucleic acid molecules cannot be amplified in a subsequent PCR reaction and cannot enter a downstream sequencing reaction, the detection performance of the target nucleic acid is finally improved, and the enrichment method is simple to operate, relatively low in cost and relatively high in detection accuracy.

The present invention relates to a lunar soil fine screening and enrichment mechanism and a lunar soil fine screening and enrichment method based on spectral selection design. The lunar soil fine screening and enrichment mechanism includes a driving part, a spectral scanning part, a feeding shell, a bearing, a collecting shell, a turntable, a protective cover, a multi-channel conveying pipe and multiple collecting boxes. The upper end of the collecting shell is an open structure, and the lower end of the collecting shell is connected to the multi-channel conveying pipe. Multiple branch pipes of the multi-channel conveying pipe are connected to multiple collection boxes in a one-to-one correspondence; the feeding shell and the protective cover are respectively fixed at the open end of the collecting shell, the turntable is located in the collecting shell and a waste collection gap is reserved between the turntable and the peripheral side wall of the collecting shell; the driving end of the driving part is connected to the center position of the turntable and drives the turntable to rotate; a sample brush that contacts the turntable is provided at the lower end of the feeding shell and / or the protective cover; the scanning end of the spectral scanning part is located above the outside of the protective cover; a plurality of fine screening holes are provided on the turntable, a first valve is provided at the bottom of each fine screening hole, and a second valve is provided in the multi-channel conveying pipe.

The application relates to the technical field of exosome extraction, and discloses an exosome enrichment chip and an enrichment method.The chip comprises a sample storage box, a sample inlet channel, a vortex reaction enrichment pool, a sample circulation channel and the like.The enrichment method can be used for single-time and high-throughput circulation enrichment of exosomes;exosome-specific enrichment materials are loaded on the filter membrane at the bottom of the vortex reaction enrichment pool, and the asymmetric impinging streams of the bilateral asymmetric sample inlet channels can be matched with the vortex motion, so that the residence time of the exosomes in the enrichment pool and the collision efficiency of the exosomes with the specific enrichment materials are improved, efficient enrichment is realized, then the sample passes through the filter membrane and enters the sample circulation channel, and is returned to the sample storage box, circulation enrichment of the exosomes in the sample is realized, and the exosome recovery rate is improved.The application can realize rapid, high-yield and high-purity enrichment of exosomes in large-volume samples such as urine, thoracic and abdominal fluid and cell culture supernatant.

A mixture of clustered and unclustered magnetic beads are generated from magnetic beads i) functionalized with a first primer of a primer set and ii) contained in a suspension. Each clustered bead includes a first amplicon attached to the first primer and a 5′-tagged second amplicon hybridized to the first amplicon. A 5′-tag of the second amplicon is a binding pair first member. Coated non-magnetic beads (including a binding pair second member coating, and having a diameter that is at least ten times larger than each magnetic bead) are introduced into the suspension. The clustered magnetic beads bind to at least some of coated non-magnetic beads to form bead-on-bead complexes. The unclustered magnetic beads remain free in the suspension, and are separated from the suspension. The 5′-tagged second amplicon is dehybridized from the first amplicon to generate single stranded clustered magnetic beads, which are then separated from the suspension.

A computer system, computer program product, and computer-implemented method for improving performance of a dialog system employing an automated virtual dialog agent. The method involves receiving a natural language request and generating a corresponding response with an automated virtual agent, automatically identifying and resolving a corresponding knowledge gap between the request and the response, and refining the automated virtual agent with the resolved knowledge gap.

The application discloses a metal wastewater separation and enrichment method and equipment based on intelligent pH value regulation, relates to the field of industrial wastewater treatment, and comprises the following steps: collecting wastewater monitoring data in a current period through an online monitoring network and sending the wastewater monitoring data to a PLC automatic control system; the PLC automatic control system makes intelligent regulation and control decisions according to the wastewater monitoring data in the current period and generates control instructions. The control instructions drive the reagent feeding system and the automatic process device to operate cooperatively, and finally ensure that the heavy metals are efficiently precipitated and separated. The application realizes precise reagent feeding and process cooperation for metal wastewater separation and enrichment, improves the control precision, processing efficiency and stability of metal separation, can realize full-process automatic control, improves the metal recovery purity and processing efficiency, reduces reagent consumption, and has self-optimization capability.

This microbe accumulation method includes first to third steps for accumulating a plurality of types of microbes. The first step is a step for readying an accumulation substrate (13) provided with a plurality of pores (131). Each of the plurality of pores (131) has an opening capable of capturing, for each of the plurality of types of microbes, at least one microbe of each respective type, and a depth extending in a direction including a vertically downward component. The second step is a step for setting a laser light radiation condition. The third step is a step for radiating laser light, through a sample, onto the plurality of pores (131) in accordance with the radiation condition. The region irradiated with the laser light does not include a photothermal conversion material. The step for performing setting includes a step for setting the intensity of non-resonant light in a non-resonant-light irradiation range such that the magnitude of a vertically downward component of a light-induced force produced by irradiation of the non-resonant light is greater than the magnitude of a vertically upward component of a buoyancy force due to a liquid sample and also greater than the magnitude of a vertically upward component of a force produced by Brownian motion of molecules in the liquid sample.

The application provides an enrichment method and application of foodborne pathogenic microorganisms in agricultural water, and belongs to the technical field of foodborne pathogenic microorganism detection. The enrichment method is that the pretreated agricultural water is filtered by a filter membrane; the filter membrane with microorganisms is eluted, the obtained eluate is separated, the obtained solid phase is a membrane bacterial component, and the obtained liquid phase is a solution containing a membrane virus component; and the filter membrane filtrate is treated by a tangential flow filter to obtain a tangential flow filtration virus component. The method can simultaneously enrich and separate various pathogenic bacteria and foodborne viruses in the water sample, shorten the enrichment treatment time, and the recovery rate of the foodborne viruses can be up to 62.60%, and the recovery rate of the pathogenic bacteria can be up to 94.24%, so that various common pathogenic bacteria, foodborne viruses and coronaviruses can be effectively enriched, and the method is applied to rapid detection of foodborne pathogenic microorganisms in agricultural water in cooperation with high-throughput sequencing technology or real-time fluorescent quantitative PCR detection technology.

The present application relates to the field of water phase Hg (II) isotope determination, and provides a water phase Hg (II) isotope in-situ enrichment method and analysis method.The present application uses a DGT device to adsorb the water body to be measured, and obtains a DGT device adsorbed with Hg (II); the adsorption film of the DGT device is an NSBA adsorption film; the NSBA adsorption film in the DGT device adsorbed with Hg (II) is eluted, and Hg (II) eluent is obtained, wherein the mercury concentration in the Hg (II) eluent needs to be greater than or equal to 0.5 ng / mL; and the eluent for the elution is reverse aqua regia.The method of the present application only needs to place the NSBA-DGT device in the water body to be measured to adsorb Hg (II) in the water body to be measured in-situ, without directly grabbing sampling, thereby reducing the pollution possibility in the sample transfer process of the water body to be measured, and when used for subsequent water phase Hg (II) isotope detection, the accuracy is high.

Provided is a gene target region enrichment method and a kit. The method comprises (1) amplifying fragmented DNA comprising a target region by means of a specific probe so as to obtain a captured-extension product, wherein the specific probe comprises a sequence complementary to the target region of the fragmented DNA, and the 3′ terminal nucleotide of the specific probe is modified to prevent a ligation reaction at the 3′ terminal of the specific probe; and (2) linking the 3′ terminal of the captured-extension product obtained in step (1) to linker DNA to obtain a ligation product.

The present invention is directed to methods for using particles (e.g, microparticulate, nanoparticulate; magnetic, non-magnetic) comprising surfaces comprising capture moieties as described herein, to remove an interference as described herein, or enrich biomarkers, prior to a diagnostic test.

The application provides a Kr enrichment method and system based on carbon molecular sieve gradient adsorption, a terminal and a storage medium, relates to the technical field of Kr enrichment, and comprises the following steps: obtaining a system trigger signal; controlling a preset cooling device to cool a preset primary adsorption column based on the system trigger signal, and obtaining a cooling completion signal; controlling a preset ventilation device to introduce a preset sample gas into the column based on the cooling completion signal, and obtaining a ventilation trigger signal; obtaining a transient flow value, a krypton inlet concentration and a krypton outlet concentration based on the ventilation trigger signal; analyzing the transient flow value and a preset standard flow value to determine a pore size detection result; analyzing the krypton inlet concentration, the krypton outlet concentration and the pore size detection result to determine actual adsorption parameters; and controlling a preset primary separation module to enrich krypton according to the actual adsorption parameters. The application has the effect of ensuring efficient enrichment of krypton by carbon molecular sieve.

A method for isolating circulating tumor cells (CTCs) from a sample comprising or suspected of comprising CTCs, the method comprising: incubating the sample with immune microparticles, thereby forming cell and microparticle aggregates (CPAs) in an incubation solution wherein the CPAs have an average size greater than 10 m; and isolating the CPA using a size-based isolation method to form an isolated CTC wherein the immune microparticle comprises one or more first antibodies conjugated to the microparticle wherein the one or more first antibodies specifically bind to one or more markers of CTC.

The invention belongs to the field of analysis of risky substances, and relates to a method for enriching risky substances in a large-volume water sample. According to the enrichment method, a micro-extraction device is adopted to enrich risky substances in a water sample to be detected, and the micro-extraction device comprises a liquid conveying device and an injector column tube; the injector column tube is filled with a solid support material, and the solid support material is loaded with a deep eutectic solvent; based on 100 mg of the solid support material, the addition amount of the deep eutectic solvent is 150-250 [mu] L; comprising the following steps: (1) adding a water sample to be detected into a liquid conveying device, enabling the water sample to flow through an injector column tube under the action of gravity, and extracting through a deep eutectic solvent loaded on a solid support material; and (2) after the extraction is finished, eluting by using an elution solvent to obtain an eluent. The device disclosed by the invention can be used for directly treating a water sample of more than or equal to 100mL, the enrichment multiple of more than hundreds of times is realized, and the application range is obviously expanded.

The invention belongs to the technical field of molecular biological detection, and particularly relates to an enrichment method and a detection method of a trace nucleic acid sample. According to the enrichment method and the detection method of the trace nucleic acid sample, the performance of an original reagent is not changed, a to-be-detected sample is divided into sub-samples, and the sub-samples are extracted in batches according to a specific proportion and a specific number, so that the loss of extraction efficiency caused by too small sample size in single treatment is avoided; the total amount of nucleic acid is increased by combining extracted products, and the ultrafiltration concentration efficiency is improved. According to the method, the sample does not need to be pretreated, nucleic acid extraction and enrichment are carried out on cfDNA, free pathogenic particles and intracellular bacteria in the sample at the same time, the real load of pathogenic microorganisms and nucleic acid in the sample is reduced to a great extent, and the method is suitable for an enrichment and extraction process of a trace nucleic acid sample and meets clinical detection requirements.

The invention provides a Kr enrichment method and system based on carbon molecular sieve gradient adsorption, a terminal and a storage medium, and relates to the technical field of Kr enrichment. Controlling a preset cooling device to cool a preset primary adsorption column based on the system trigger signal, and obtaining a cooling completion signal; controlling a preset ventilation device to introduce preset sample gas into the column based on the cooling completion signal, and obtaining a ventilation trigger signal; acquiring an instantaneous flow value, krypton inlet concentration and krypton outlet concentration based on the ventilation trigger signal; analyzing the instantaneous flow value and a preset standard flow value to determine an aperture detection result; analyzing krypton inlet concentration, krypton outlet concentration and aperture detection results to determine actual adsorption parameters; and controlling a preset primary separation module to enrich krypton according to the actual adsorption parameters. The method has the effect of ensuring efficient enrichment of krypton by the carbon molecular sieve.

A method is provided for enriching extracellular DNA from a biological sample comprising extracellular DNA and extracellular vesicles, wherein the method comprises: (a) preparing a binding mixture comprising—the biological sample, —a solid phase comprising anion exchange groups, —an acidic binding buffer comprising a buffering agent, and binding extracellular DNA to the solid phase comprising anion exchange groups; (b) separating the solid phase with the bound extracellular DNA from the remaining binding mixture, wherein the remaining binding mixture comprises extracellular vesicles. The method may furthermore comprise processing the remaining binding mixture to enrich one or more biological targets of interest therefrom, wherein processing may comprise (c) enriching as biological targets extracellular vesicles and / or extracellular RNA from the remaining binding mixture.