40 results about "Enrichment methods" patented technology

The invention discloses a micro-fluidic chip-based trace tumor nucleic acid low-damage enrichment method and system, and the method comprises the steps: constructing a virtual flow channel network digital twin model of a multi-stage micro-fluidic chip, the virtual flow channel network digital twin model comprises enrichment units connected in series, each enrichment unit is composed of a tapered convergence section, a periodic bending section and an aptamer modified amplification section; and executing real-time simulation of fluid dynamics based on the digital twinborn model, generating a dynamic pressure control instruction set, and adjusting the flow velocity distribution of the biological sample in the flow channel through a negative pressure system driven by a fuzzy PID (Proportion Integration Differentiation) controller. According to the invention, through digital twin dynamic reconstruction flow channel and multi-modal fusion regulation, shear stress accumulation is inhibited to maintain nucleic acid integrity, impurity adsorption is reduced based on dynamic balance of probe density and binding saturation, and multi-field collaborative optimization is realized.

The object of the present invention is to provide a stable isotope enrichment method that can reduce equipment costs and power without lengthening the start-up time and can achieve efficient enrichment. The present invention provides a stable isotope enrichment method, which is a method for separating stable isotopes using a plurality of distillation columns (the first column to the m-th column; m is an integer of 2 or more) connected in cascade. One of gas and liquid is supplied from a position near the bottom of the (n-1)-th column to a position near the top of the n-th column (1 < n ≤ m), and the other of gas and liquid is returned from a position near the top of the n-th column to a position near the bottom of the (n-1)-th column. In each distillation column, when the flow rate of the gas rising in the column is set as the first flow rate and the flow rate of the gas or liquid supplied from the previous column or the next column is set as the second flow rate, the second flow rate is set to 4% by volume or more of the first flow rate.

This application relates to an in-situ self-aeration enrichment device for microorganisms in the vadose zone of contaminated soil, comprising an in-situ enrichment module, a filling matrix, and a composite nutrient additive. The in-situ enrichment module consists of a cuboid, a smooth frustum, and a long cylindrical air-guiding channel, which are integrally formed from bottom to top. The filling matrix is ​​placed into the enrichment module through a fine-mesh mesh bag. The composite nutrient additive is injected into the enrichment module through the long cylindrical air-guiding channel. This enrichment device improves the enrichment efficiency of uncultured microorganisms in the vadose zone of organically contaminated sites through the combined action of the in-situ enrichment module, the filling matrix, and the composite nutrient additive.

The invention discloses a targeted enrichment method for proteome. According to the cell nucleus targeted RNA binding protein enrichment reagent, one end is provided with a furocoumarin group, the other end is provided with a cell nucleus targeted group, and the other end is provided with biotin; the enrichment or identification of the RNA binding protein in the cell nucleus can be carried out. The method for enriching the nucleus-targeted RNPs by adopting the nucleus-targeted RNA binding protein enrichment reagent comprises the following steps: firstly, adding the nucleus-targeted RNA binding protein enrichment reagent into cells for co-incubation, then, irradiating a cell incubation system by adopting ultraviolet light with the wavelength of 254nm, and then, irradiating the cell incubation system by adopting ultraviolet light with the wavelength of 365nm, then carrying out cracking to obtain a cracking solution; incubating the lysis solution and streptavidin magnetic beads, and finally collecting the magnetic beads enriched with the cell nucleus RNPs, so that the cell nucleus targeted RNPs are enriched. According to the method, the cell nucleus positioning of the reagent can be efficiently realized, the RBPs enrichment selectivity and the negative electrode efficiency are improved, and the varieties of RNPs obtained through enrichment are more comprehensive.

To provide an oxygen isotope concentrator where the reduction of an oxygen treatment amount can be performed by simplifying device configuration.SOLUTION: An oxygen isotope concentrator 1 comprises: a plurality of device groups having an ozone generation device 11, a carbon tetrafluoride feeder 17, an oxygen separator 12, an ozone photolysis device 13, a collector 14 and a concentrator 15; one or more oxygen supply passages 16 introducing oxygen separated at the concentrator 15 of the kth group (1≤k≤(n-1)) to the ozone generation device 11 of the (k+1)th group; and one or more mixed gas return passages 23' by which a mixed gas of ozone separated at the concentrator 15 of any jth group (2≤j≤n) and carbon tetrafluoride is introduced to any one of the first group to the (j-1) group.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of analytical chemistry, and particularly relates to a preparation method of a directional pore channel integral material and a method for constructing an enrichment device and establishing a rapid phosphopeptide enrichment method by using the directional pore channel integral material. The preparation method comprises the following steps: preparing a CS-PC integral material with directional pore channels from chitosan, zirconium amino phthalocyanine and polyethylene glycol diglycidyl ether through a directional freezing method and a chemical crosslinking method, and then modifying the CS-PC integral material with 5 '-phosphopyridoxal and zirconium oxychloride to obtain the CS-PC PLP integral material. The CS-PC-PLP integral material prepared by the invention has anisotropy and a directional pore channel structure, is convenient to fix in a pipette tip and is combined with a row pipettor for rapid enrichment; the CS-PC coated PLP integral material has the advantages of green and cheap raw materials and strong reusability.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

The invention provides a group of enrichment primers, a pathogenic microorganism enrichment kit, a pathogenic microorganism enrichment method and related application. The enrichment primer provided by the invention is as shown in a table 1, the amplification fragment of the enrichment primer and the enrichment kit provided by the invention on target pathogenic microorganisms is about 1kb, and compared with a conventional short amplification fragment, the detection accuracy of a long sequence amplified by the enrichment primer and the enrichment kit is higher. According to the enrichment method disclosed by the invention, the read length of a sequencing fragment is increased, and the capability of precise typing of microorganisms and drug-resistant / virulence genes is further improved. The enrichment primer, the kit and the enrichment method can be combined with a single molecule sequencing technology, the sequencing period is shortened, sample sequencing can be completed within one hour, the sample detection period is greatly shortened, and the enrichment primer, the kit and the enrichment method are more suitable for the field of clinical pathogen rapid detection.

A method for isolating a circulating tumor cell (CTC) in a sample comprising the CTC or suspected of comprising the CTC, the method including: incubating the sample with immuno-microparticles thereby forming cell and microparticle aggregates (CPAs) in an incubation solution, wherein the average size of the CPAs is larger than 10 μm; and isolating the CPAs with a size-based isolation method thereby forming an isolated CTC, wherein the immuno-microparticles comprise one or more first antibodies conjugated to microparticles, wherein the one or more first antibodies specifically bind to one or more markers of the CTC.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

The present disclosure provides methods and systems for capturing and enriching nucleic acid sequences. Probes or primers may be used to capture or enrich nucleic acids. Characteristics of the probe or primer may be adjusted or tuned to produce a sequencing depth for a given region. The sequencing depth may be non-uniform between genomic regions.

The invention relates to an enrichment method and application of antimony oxidizing microorganisms, and belongs to the technical field of microorganisms. According to the enrichment method, on the premise that active cells are reserved, the antimony oxidation function microbiota in the solid sample microbe seed bank is efficiently enriched, the influence of a matrix on the enrichment result and the activity of the antimony oxidation microbiota can be effectively reduced, and the enrichment method has the advantages of being convenient, efficient and small in error and has a certain pollution treatment application prospect.

The invention discloses a method for efficiently enriching uranyl in a fiber eluent for extracting uranium from seawater, and belongs to the technical field of extracting uranium from seawater. According to the method, an ion exchange method and a chemical precipitation method are adopted for treating an eluent of seawater uranium extraction fibers, the fiber eluent is pretreated firstly, then D201 resin is used for concentrating and purifying the fiber eluent to obtain a uranium concentrated solution, finally, ammonia water is used for precipitating the uranium concentrated solution, precipitates are collected, and the precipitates are centrifugally dried to obtain the uranium compound. According to the method, uranyl in the fiber eluent can be efficiently enriched.

The invention discloses a nucleic acid reverse enrichment method which comprises the following steps: extracting and purifying nucleic acid in a sample to obtain a nucleic acid sample, and if the nucleic acid is RNA (Ribonucleic Acid), carrying out reverse transcription on the purified RNA to obtain cDNA (Complementary Deoxyribose Nucleic Acid); the method comprises the following steps: carrying out nucleic acid fragmentation, terminal repair and linker connection on a nucleic acid sample, inactivating or removing ligase, adding an RNA probe specifically bound with non-target DNA, and carrying out hybridization annealing to obtain a mixture; adding enzyme for cutting a DNA chain in the DNA-RNA hybrid compound into the mixture for incubation and magnetic bead purification; carrying out PCR amplification and magnetic bead purification on the purified nucleic acid; according to the method, non-target nucleic acid molecules are cut off by using specific endonuclease, the non-target nucleic acid molecules cannot be amplified in a subsequent PCR reaction and cannot enter a downstream sequencing reaction, the detection performance of the target nucleic acid is finally improved, and the enrichment method is simple to operate, relatively low in cost and relatively high in detection accuracy.

The present invention relates to a lunar soil fine screening and enrichment mechanism and a lunar soil fine screening and enrichment method based on spectral selection design. The lunar soil fine screening and enrichment mechanism includes a driving part, a spectral scanning part, a feeding shell, a bearing, a collecting shell, a turntable, a protective cover, a multi-channel conveying pipe and multiple collecting boxes. The upper end of the collecting shell is an open structure, and the lower end of the collecting shell is connected to the multi-channel conveying pipe. Multiple branch pipes of the multi-channel conveying pipe are connected to multiple collection boxes in a one-to-one correspondence; the feeding shell and the protective cover are respectively fixed at the open end of the collecting shell, the turntable is located in the collecting shell and a waste collection gap is reserved between the turntable and the peripheral side wall of the collecting shell; the driving end of the driving part is connected to the center position of the turntable and drives the turntable to rotate; a sample brush that contacts the turntable is provided at the lower end of the feeding shell and / or the protective cover; the scanning end of the spectral scanning part is located above the outside of the protective cover; a plurality of fine screening holes are provided on the turntable, a first valve is provided at the bottom of each fine screening hole, and a second valve is provided in the multi-channel conveying pipe.

The invention belongs to the technical field of molecular biology, and particularly relates to an efficient free mitochondrial DNA enrichment method, a kit and application thereof. According to the enrichment method disclosed by the invention, the bait library generated by PCR amplification and the free mitochondrial DNA are subjected to efficient capture hybridization, so that the enrichment effect of the target mitochondrial DNA can be remarkably improved, and particularly, enough mitochondrial DNA can be captured for analysis in a sample with relatively low concentration of the free DNA, so that false negative or low-efficiency detection possibly occurring in a traditional method is avoided; the capture efficiency and influence factors of the enrichment method are verified, and the coverage range of the target DNA amplified and enriched in the serum and urine samples is within the whole mtDNA genome, so that the enrichment method effectively improves the quality of the enrichment library; and a more reliable technical support is provided for efficient capture and sequencing of the free mitochondrial DNA.

The application relates to the technical field of exosome extraction, and discloses an exosome enrichment chip and an enrichment method.The chip comprises a sample storage box, a sample inlet channel, a vortex reaction enrichment pool, a sample circulation channel and the like.The enrichment method can be used for single-time and high-throughput circulation enrichment of exosomes;exosome-specific enrichment materials are loaded on the filter membrane at the bottom of the vortex reaction enrichment pool, and the asymmetric impinging streams of the bilateral asymmetric sample inlet channels can be matched with the vortex motion, so that the residence time of the exosomes in the enrichment pool and the collision efficiency of the exosomes with the specific enrichment materials are improved, efficient enrichment is realized, then the sample passes through the filter membrane and enters the sample circulation channel, and is returned to the sample storage box, circulation enrichment of the exosomes in the sample is realized, and the exosome recovery rate is improved.The application can realize rapid, high-yield and high-purity enrichment of exosomes in large-volume samples such as urine, thoracic and abdominal fluid and cell culture supernatant.

The present invention provides a continuous on-line enrichment analysis device for ODS and fluorinated greenhouse gases, which includes a six-way valve 1 and a six-way valve 2, an enrichment trap T1 connected to the six-way valve 1 and an enrichment trap T2 connected to the six-way valve 2. The six-way valve 1 is connected to a NAFION tube through the NAFION tube outlet (1a), and the NAFION tube inlet is the sample gas inlet. The carrier gas inlet (1e) on the six-way valve is connected to the carrier gas. The six-way valve 1 is connected to an MFC through the MFC inlet (2b), and the outlet of the MFC is connected to a sampling pump. The six-way valve 2 is connected to a GCMS through the carrier gas outlet (2d). An anti-CO2 tube and a damping column are connected between the six-way valve 1 and the six-way valve 2. The device of the present invention adopts a two-stage cold trap enrichment method, with high enrichment efficiency. The two enrichment traps are respectively controlled by different refrigerators without interference with each other, ensuring the stability of the analysis process and enabling accurate measurement of ODS and fluorinated greenhouse gases.

A mixture of clustered and unclustered magnetic beads are generated from magnetic beads i) functionalized with a first primer of a primer set and ii) contained in a suspension. Each clustered bead includes a first amplicon attached to the first primer and a 5′-tagged second amplicon hybridized to the first amplicon. A 5′-tag of the second amplicon is a binding pair first member. Coated non-magnetic beads (including a binding pair second member coating, and having a diameter that is at least ten times larger than each magnetic bead) are introduced into the suspension. The clustered magnetic beads bind to at least some of coated non-magnetic beads to form bead-on-bead complexes. The unclustered magnetic beads remain free in the suspension, and are separated from the suspension. The 5′-tagged second amplicon is dehybridized from the first amplicon to generate single stranded clustered magnetic beads, which are then separated from the suspension.

The invention discloses an in-situ germanium concentration method based on GeOx / SiGe stacking and application of the in-situ germanium concentration method. The method comprises the following steps: firstly, sequentially epitaxially growing a SiGe layer and a GeOx layer on a silicon-based substrate to form a GeOx / SiGe stacking structure; and then annealing in an inert gas atmosphere, enabling GeOx to react with silicon atoms in the SiGe layer through thermodynamic driving, enabling the silicon atoms in the SiGe layer to replace germanium atoms in the GeOx layer to form SiOx, and directionally migrating the reduced and replaced germanium atoms to lattice positions of the silicon atoms in the SiGe layer so as to realize in-situ germanium concentration. The method breaks through the critical thickness limitation of traditional silicon-germanium epitaxy, and compared with a traditional oxidation concentration process, the problems of germanium atom efflux loss and surface roughening are avoided. In-situ redistribution of germanium atoms is realized through solid-state reaction, and the percentage of the germanium atoms can be increased to 35%-45%; the method is compatible with an existing CMOS production line, the process temperature is reduced by 300-550 DEG C compared with a traditional method, the method is particularly suitable for channel engineering of high-node devices such as 3D FinFET fins and nanosheets, and the problem that germanium is not evenly distributed in a fin / nanosheet structure is solved.

A computer system, computer program product, and computer-implemented method for improving performance of a dialog system employing an automated virtual dialog agent. The method involves receiving a natural language request and generating a corresponding response with an automated virtual agent, automatically identifying and resolving a corresponding knowledge gap between the request and the response, and refining the automated virtual agent with the resolved knowledge gap.

This lithium-isotope enrichment device comprises a treatment tank which is divided into a supply tank and a recovery tank by means of an electrolyte membrane having lithium ion conductivity, and recovers, into the recovery tank, an aqueous solution ES for the recovery of 6Li of which the isotope ratio of 6Li is high from a Li-containing aqueous solution FS stored in the supply tank. While a power supply, which is connected between a second electrode of a porous structure provided on a recovery tank-side surface of the electrolyte membrane and a third electrode provided to be spaced apart from the electrolyte membrane in the recovery tank, applies a voltage V1 with the second electrode being made to be positive, the lithium-isotope enrichment device connects a first electrode provided in the supply tank to the second electrode.

The application discloses a metal wastewater separation and enrichment method and equipment based on intelligent pH value regulation, relates to the field of industrial wastewater treatment, and comprises the following steps: collecting wastewater monitoring data in a current period through an online monitoring network and sending the wastewater monitoring data to a PLC automatic control system; the PLC automatic control system makes intelligent regulation and control decisions according to the wastewater monitoring data in the current period and generates control instructions. The control instructions drive the reagent feeding system and the automatic process device to operate cooperatively, and finally ensure that the heavy metals are efficiently precipitated and separated. The application realizes precise reagent feeding and process cooperation for metal wastewater separation and enrichment, improves the control precision, processing efficiency and stability of metal separation, can realize full-process automatic control, improves the metal recovery purity and processing efficiency, reduces reagent consumption, and has self-optimization capability.

This microbe accumulation method includes first to third steps for accumulating a plurality of types of microbes. The first step is a step for readying an accumulation substrate (13) provided with a plurality of pores (131). Each of the plurality of pores (131) has an opening capable of capturing, for each of the plurality of types of microbes, at least one microbe of each respective type, and a depth extending in a direction including a vertically downward component. The second step is a step for setting a laser light radiation condition. The third step is a step for radiating laser light, through a sample, onto the plurality of pores (131) in accordance with the radiation condition. The region irradiated with the laser light does not include a photothermal conversion material. The step for performing setting includes a step for setting the intensity of non-resonant light in a non-resonant-light irradiation range such that the magnitude of a vertically downward component of a light-induced force produced by irradiation of the non-resonant light is greater than the magnitude of a vertically upward component of a buoyancy force due to a liquid sample and also greater than the magnitude of a vertically upward component of a force produced by Brownian motion of molecules in the liquid sample.

The application provides an enrichment method and application of foodborne pathogenic microorganisms in agricultural water, and belongs to the technical field of foodborne pathogenic microorganism detection. The enrichment method is that the pretreated agricultural water is filtered by a filter membrane; the filter membrane with microorganisms is eluted, the obtained eluate is separated, the obtained solid phase is a membrane bacterial component, and the obtained liquid phase is a solution containing a membrane virus component; and the filter membrane filtrate is treated by a tangential flow filter to obtain a tangential flow filtration virus component. The method can simultaneously enrich and separate various pathogenic bacteria and foodborne viruses in the water sample, shorten the enrichment treatment time, and the recovery rate of the foodborne viruses can be up to 62.60%, and the recovery rate of the pathogenic bacteria can be up to 94.24%, so that various common pathogenic bacteria, foodborne viruses and coronaviruses can be effectively enriched, and the method is applied to rapid detection of foodborne pathogenic microorganisms in agricultural water in cooperation with high-throughput sequencing technology or real-time fluorescent quantitative PCR detection technology.

The present application relates to the field of water phase Hg (II) isotope determination, and provides a water phase Hg (II) isotope in-situ enrichment method and analysis method.The present application uses a DGT device to adsorb the water body to be measured, and obtains a DGT device adsorbed with Hg (II); the adsorption film of the DGT device is an NSBA adsorption film; the NSBA adsorption film in the DGT device adsorbed with Hg (II) is eluted, and Hg (II) eluent is obtained, wherein the mercury concentration in the Hg (II) eluent needs to be greater than or equal to 0.5 ng / mL; and the eluent for the elution is reverse aqua regia.The method of the present application only needs to place the NSBA-DGT device in the water body to be measured to adsorb Hg (II) in the water body to be measured in-situ, without directly grabbing sampling, thereby reducing the pollution possibility in the sample transfer process of the water body to be measured, and when used for subsequent water phase Hg (II) isotope detection, the accuracy is high.

The invention relates to the method of enriching the carbon content of solid wastes, which are discarded after obtaining energy from animal wastes, by flotation The products obtained by this method are used in different areas of the industry. 0 5 0 5 0

The invention discloses an enrichment system and an enrichment method of < 7 > Li isotope. The enrichment system comprises a lithium salt aqueous solution, a < 7 > Li enrichment aqueous solution and at least one composite separation membrane; the concentration of lithium ions in the lithium salt aqueous solution is 0.005 mol / L to 3.00 mol / L, and the composite separation membrane comprises a functional membrane and at least one support membrane; wherein the functional film comprises a plurality of pore channels penetrating through the first surface and the second surface of the functional film, and the pore channel diameter of each pore channel is 0.8-1.5 nm; a lithium ion trapping functional group is modified at an inlet of the first surface of the pore channel; the composite separation membrane is arranged between the lithium salt aqueous solution and the 7Li-enriched aqueous solution, and the second surface in the composite separation membrane is farther from the lithium salt aqueous solution than the first surface. The lithium isotope separation effect does not change along with the migration time and the lithium ion migration proportion, and long-term continuous stability is achieved.

Provided is a gene target region enrichment method and a kit. The method comprises (1) amplifying fragmented DNA comprising a target region by means of a specific probe so as to obtain a captured-extension product, wherein the specific probe comprises a sequence complementary to the target region of the fragmented DNA, and the 3′ terminal nucleotide of the specific probe is modified to prevent a ligation reaction at the 3′ terminal of the specific probe; and (2) linking the 3′ terminal of the captured-extension product obtained in step (1) to linker DNA to obtain a ligation product.

The present invention is directed to methods for using particles (e.g, microparticulate, nanoparticulate; magnetic, non-magnetic) comprising surfaces comprising capture moieties as described herein, to remove an interference as described herein, or enrich biomarkers, prior to a diagnostic test.