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Method for compressing marine microalgae

A marine microalgae and microalgae technology, applied in botany equipment and methods, plant preservation, animal feed, etc., can solve the problems of large damage to microalgae, high ionic strength, high operating costs, etc., and achieve rapid flocculation effect and preparation Simple solution, the effect of improving immunity

Inactive Publication Date: 2008-01-02
MARICULTURE INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly two methods for concentrating microalgae in actual production. One is physical methods, including ultrafiltration and centrifugation. This concentration method has a large initial investment cost and high operating costs. Small-scale production
The second is chemical methods, which generally use flocculants such as lime water, alum, polyiron or polyaluminum. These flocculation methods are difficult to apply in aquatic products because of the greater damage to microalgae and the toxicity of flocculant residues to seedlings.
Sun Jianhua used polyacrylamide, chitin, and alginate as flocculants, but their effect on sedimentation of marine microalgae was poor. It may be related to the high ionic strength in seawater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment 1: N-deacetylation degree is 70%, viscosity (referring to 1 gram chitosan is dissolved in 100 milliliters of volume percent concentration and is the viscosity after 1% acetic acid aqueous solution, the same below) is 700 * 10 -3 Pa·s chitosan was dissolved in acetic acid. The acetic acid solution of this chitosan is added in the flat algae culture liquid, the chitosan concentration in this flat algae culture liquid is 0.3~8.0mg / L, the pH value of adjusting this flat algae culture liquid is 4.5~8.5, the The flat algae is completely flocculated in 0.1-1 hour, and the flocculation degree is above 95%. After static stratification, the supernatant is poured out to collect the flocs.

Embodiment 2

[0008] Embodiment 2: N-deacetylation degree is 80%, viscosity is 700×10 -3 Pa·s chitosan was dissolved in acetic acid. This chitosan acetic acid solution is added in the flat algae culture fluid, the chitosan concentration in this flat algae culture fluid is made to be 0.3~8.0mg / L, and the pH value of this adjustment of this flat algae culture fluid is 4.5~8.5, and the flat algae culture fluid is The algae are completely flocculated within 0.1-1 hour, and the degree of flocculation is above 95%. After static stratification, the supernatant is poured off, and the flocs are collected.

Embodiment 3

[0009] Embodiment 3: N-deacetylation degree is 90%, viscosity is 700×10 -3 Pa·s chitosan was dissolved in acetic acid. This chitosan acetic acid solution is added in the flat algae culture fluid, the chitosan concentration in this flat algae culture fluid is made to be 0.3~8.0mg / L, and the pH value of this adjustment of this flat algae culture fluid is 4.5~8.5, and the flat algae culture fluid is The algae are completely flocculated within 0.1-1 hour, and the degree of flocculation is above 95%. After static stratification, the supernatant is poured off, and the flocs are collected.

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Abstract

The invention relates to a method for condensing ocean micro-algae, which comprises following steps: adding organic acid solution of chitosan into micro-algae culture solution to make the chitosan solution concentration in micro-algae culture solution be 0.3-8.0 mg / l, adjusting micro-algae culture solution pH to be 4.5-8.5 for micro-algae flocculation, collecting floccule with static hierarchical or gas-floating method. The invention is characterized by non-toxic chitosan, simple solution preparation, fast flocculation speed, good activity of flocculated micro-algae. The micro-algae can be dispersed into single cells in water after a certain time of storage, and can be used for breeding as algae seed. The chitosan can improve the animal immunity, and micro-algae can be used in foodstuff and medical treatment field.

Description

technical field [0001] The invention relates to a chemical concentration method for marine microalgae. Background technique [0002] Microalgae (Micmalgae), also known as single-cell algae, has been widely used in aquaculture, sewage purification, food, feed, medicine and other industries because of its high efficiency in utilizing solar energy, rich nutrition, vigorous growth and reproduction, strong adaptability, and easy cultivation. . Microalgae are widely used as bait for aquatic animal larvae (such as shellfish larvae, zoan larvae and mysis larvae of prawns, sea cucumber salivary larvae, etc.) The field is fed directly with fresh algae liquid (microalgae and culture liquid), and the number of cultured microalgae and feeding time must match the seedling needs. loss. If there is an ideal method for concentrating and storing microalgae, it can be used when fresh algae liquid is in short supply, which can solve the problem of untimely and unstable bait cultivation affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/18A01N3/00A23K10/30
Inventor 詹冬梅杨秀生
Owner MARICULTURE INST OF SHANDONG PROVINCE
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