Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High performance quick purifying method for preparing piecewise antibody

A technology of antibodies and fragments, applied in the field of bioengineering, to achieve high purity, low cost, and small difference in results

Active Publication Date: 2005-01-12
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF2 Cites 60 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Sullivan; John B, Russell; Findlay E. In the patent related to antibody purification process (United States Patent, No 4,849,352) [4], it is described that papain-cleaved Fab fragment antibody is separated by polyphenylacrylamide gel affinity chromatography and pepsin-cleaved F(ab') 2 Antibodies, the conditions and methods of the purification process are not specifically described

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High performance quick purifying method for preparing piecewise antibody
  • High performance quick purifying method for preparing piecewise antibody
  • High performance quick purifying method for preparing piecewise antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0062] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments given by the inventor.

[0063] According to the technical scheme of the present invention, the applicant uses the monoclonal antibody secreted by hybridoma cells to carry out F(ab') 2 Preparation of fragment antibodies. The hybridoma cell stably secretes and expresses a monoclonal antibody against human liver cancer-related antigen (preserved by the General Microorganism Collection Center of China Committee for Culture Collection of Microorganisms, deposit number: CGMCC No 0426).

[0064] Hybridoma cells were cultured in large-scale high-density suspension in a mechanically stirred bioreactor; the antibody IgG in the large-scale cell culture medium in the early stage was adsorbed by expanded column bed cation chromatography and concentrated by anion chromatography. Elution was carried out by changing the pH of the buffer system. Antib...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for high-effectively quickly purifying and preparing fragment antibody from animal cell scale culture. Said method adopts the processes of dilatant column bed adsorption, anion chromatography concentration and hydrophobic chromatography purification, and includes the following steps: using dilatant column bed cation chromatography to adsorb antibody IgG from large-scale cell culture, changing pH value of buffer system to make elution, making collected elution peak be directly andergone the process of anion chromatography, using pepsinum to directly enzyme-out the fully-concentrated and purified antibody IgG to obtain fragment antibody, making hydrophobic chromatography and purification so as to obtain final fragment antibody whose purity is up to above 97%, and its whole purification process can be completed within 12 hr. Said invented method can be substituted for traditional ammonium sulfate precipitation, concentration method, and has the characteristics of high efficiency, rapid speed, high purity and stable operation and result, etc.

Description

technical field [0001] The invention belongs to the protein purification technology in the technical field of bioengineering, and mainly relates to the purification and preparation process of antibodies including monoclonal antibodies and small molecule fragment antibodies, humanized or human-mouse chimeric antibodies and fragment antibodies. In particular, it relates to a method for efficiently and quickly purifying and preparing fragment antibodies, which is suitable for the production and preparation of therapeutic antibody drugs. Background technique [0002] Antibody therapy has become an important means of biological treatment of tumors, epidemics and infectious diseases today. As of February 2004, the US FDA has approved 16 antibody therapeutic drugs for marketing, and the market sales have exceeded 2×10 9 dollars. Antibody drugs have become a large class of new therapeutic agents in the international pharmaceutical market due to their high specificity, effectivenes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K1/16C07K16/18
Inventor 米力唐浩余晓玲谢丽
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com