758 results about "Ionic chromatography" patented technology

The invention discloses a method for high-effectively quickly purifying and preparing fragment antibody from animal cell scale culture. Said method adopts the processes of dilatant column bed adsorption, anion chromatography concentration and hydrophobic chromatography purification, and includes the following steps: using dilatant column bed cation chromatography to adsorb antibody IgG from large-scale cell culture, changing pH value of buffer system to make elution, making collected elution peak be directly andergone the process of anion chromatography, using pepsinum to directly enzyme-out the fully-concentrated and purified antibody IgG to obtain fragment antibody, making hydrophobic chromatography and purification so as to obtain final fragment antibody whose purity is up to above 97%, and its whole purification process can be completed within 12 hr. Said invented method can be substituted for traditional ammonium sulfate precipitation, concentration method, and has the characteristics of high efficiency, rapid speed, high purity and stable operation and result, etc.

LC/MS data generated by an LC/MS system is analyzed to determine groupings of ions associated with originating molecules. Ions arc grouped initially according to retention time, for example, using retention time or chromatographic peaks in mass chromatograms. After initial groupings are determined based on retention time, ion peak shapes are compared to determine whether ions should be excluded. Ions having peak shapes not matching other ions, or alternatively a reference peak shape, are excluded from the group.

The invention discloses a method for determining content of potassium, sodium, calcium and magnesium in cigarette paper simultaneously, which includes the following steps: cutting 100+/-0.1 mg of a cigarette paper sample, using 100mL of 0.02mol/L hydrochloric acid as an extracting agent, ultrasonic-extracting for 18-22min to obtain extract liquor, selecting 5.0mL of the extract liquor to a 25mL volumetric flask, bringing to the volume with 0.02mol/L hydrochloric acid to obtain diluent, and filtering the diluent through a 0.45 mu m filtering film to obtain sample liquid to be detected; selecting sodium chloride, potassium chloride, hexahydrate magnesium chloride and anhydrous calcium chloride, using pure water as solvent, preparing mixed standard solution through gradual diluting, and adding 0.02mol/L hydrochloric acid to prepare series standard working solution; detecting and analyzing the series working solution and the sample liquid to be detected respectively through an ion chromatographic instrument; and drawing a standard curve and calculating results. The method is fast and simple to operate, high in sensitivity, low in detection limit, good in recovery rate, and suitable for batch analyzing of important inorganic elements in the cigarette paper.

The invention relates to a multi-dimensional ion chromatographic analysis system, belonging to the chemical field of analysis. To solve the problem that a conventional chromatographic technique is insufficient in separation of molecules such as various organic acid, amino acid, sugar and alcohol in complex samples, a set of multi-dimensional ion chromatographic analysis system is developed; the system comprises a plurality of pumps, one or more changeover valves and a plurality of detectors; by utilizing the difference of separation mechanisms of target components on chromatographic columns and the difference of retention time of the components, and through a plurality of chromatographic switchover enrichments and valve switchover time selection, ion chromatographic connected systems of two-dimension, three-dimension, four-dimension, five-dimension and higher can be established according to difference of target components to be separated, so that the coseparation analysis of water-soluble metabolite such as organic acid, amino acid, sugar and alcohol of complex samples is realized. By utilizing the multi-dimensional ion chromatographic analysis system, the complex sample can be rapidly, sufficiently and precisely separated and identified, and the mutual interference among the components is eliminated.

The invention relates to an ion chromatography single-pump column switching system which mainly comprises a pump, a guard column, an analytical column, a suppressor, a conductivity detector, a sample loop, a ten-way valve, an ion exclusion column, a waste liquid barrel, a six-way valve, an enrichment column and other parts, wherein the pump is connected with the six-way valve; the enrichment column is connected on the six-way valve; the six-way valve is respectively connected with the guard column, the ion exclusion column and the waste liquid barrel; the analytical column is respectively connected with the guard column and the suppressor; the suppressor is respectively connected with the analytical column and the conductivity detector; the sampling loop is connected on the ten-way valve; and the ten-way valve is respectively connected with a conductivity flow cell, the conductivity detector, the ion exclusion column and the waste liquid barrel. The ion chromatography single-pump column switching system can ensure the analytical effect of conventional anions and realize the separation and the monitoring of the conventional anions in a high-concentration organic acid substrate by using one ion chromatography instrument, as well as simultaneously simplify the technological process, reduce chromatography accessories and save solvent consumption.

The invention relates to an anion chromatographic column a preparation method thereof, particularly relates to a preparation method of a surface-grafting anion chromatography stationary phase. The method disclosed herein is characterized by: preparing monodispersed linear polystyrene microspheres seeds by dispersion polymerization, activating the seeds, then synthesizing polystyrene-divinyl benzene-glycidyl methacrylate microballoons by one-step seed swelling method, and extracting to remove the pore forming agent; adopting multi-step grafting synthetic method, taking methylamine and 1,4-butanediol diglycidyl ether as raw materials, and introducing a large amount of positively charged quaternary ammonium groups as the anion exchange functional group to the surface of the prepared polystyrene-divinyl benzene-glycidyl methacrylate microballoons; and packing by homogenate method. According to the invention, the method disclosed herein has the advantages of low cost and simple process, the prepared filling material has uniform granularity and has no need to be screened, and the particle size distribution is narrow.

The present invention provides a method for preparing nanometer micron composite spheres, a nanometer particle having surface function group is linked to the specialised spheres surface by chemical method, so as to prepare stable nanometer micron composite spheres, and enhance the application and life of the composite spheres, these nanometer micron composite spheres are used for high-effect ion chromatography fillings, biomolecule separation and analyzing medium, cell separating magnetic spheres, catalyst and zymophore, diagnostic reagent, medical controlled released carrier etc.

The invention discloses a method for measuring trace chloridion and sulfate radical in loprazolam samples by ion chromatography and belongs to the field of analytic chemistry. The method includes the followings steps of preparing and analyzing Cl- and SO42- mixed standard solution and obtaining a linear equation of concentration and peak area, pretreating the loprazolam samples, feeding the treated loprazolam samples to an ion chromatography by a disposable needle type filter, utilizing 3.6mmol/L sodium carbonate as leacheate and analyzing and measuring the content of the chlorodion and the sulfate radical according to the obtained linear equation, wherein a chromatographic column adopted in the ion chromatography is a high-capacity chromatographic column, the inner diameter of filler of the chromatographic column is 5 micrometers, the temperature of a column temperature box is set to be 45 DEG C, sampling input quantity is 100 microliters, applied curb current is 40mA, and flow velocity is 0.8ml/min. The method is quick and simple, high in accuracy, convenient and easy to operate and applicable to quality control in the process of production of loprazolam, the integral analyzing process can be completed within 25min, and the detection requirements of the fine chemical industry are met.

A sandwich suppressor in an ion chromatography system in which loosely packed ion exchange resin of low density is disposed in the central sample stream flow channel. Also, a method of using the suppressor is described.

The invention discloses a method for simultaneously determining sweetening agents and preservatives in tobacco essence, and belongs to the technical field of chemical component detection of auxiliary materials for tobaccos. The method comprises the following steps that 1, a sample to be determined is diluted, the pH value of a solution is regulated to be 9-10, extraction is performed, extraction liquid is purified through an Oasis HLB column, and the purified extraction liquid is filtered for standby application; 2, filtrate is taken to be subjected to ion chromatographic analysis, and the content of the sweetening agents and the preservatives in the sample to be determined is calculated by contrasting a standard curve. According to the method, impurities disturbing determination in the essence are purified and removed in a classifying mode according to the physical and chemical properties of different types of the tobacco essence, one-time sample feeding is performed on the three sweetening agents (sodium cyclamate, acesulfame and saccharin sodium) and the two preservatives (sorbic acid and benzoic acid) through an AS17C analytical column and an electrical conductivity detector, and separation and determination are simultaneously performed. Compared with an industrially recommended determination method, the method has the advantages of being wide in linear range, low in quantitation limit, little in sampling quantity, high in determination efficiency and the like.

The invention relates to a detection method of selenium form in an aquatic product, and belongs to the technical field of aquatic product detection. The selenium form in the aquatic product is extracted by oscillating with a super constant-temperature mixer and measured by a high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy combined morphological analysis technology, the extracting solution is separated by an anion chromatographic column, different forms of selenium are eluted successively by a moving phase, hydrogenation is carried out on the elution solution through a potassium borohydride reducing agent and hydrochloric acid to generate a hydride which enters an atomizer and is used in combination with atomic fluorescence for analysis and measurement, so that a more complete biological property of the selenium is provided. The experiment condition is reasonable, the detection data are accurate and reliable, the sample recovery rate is 88.79-94.88% and the degree of precision is less than 5%. The method is high in sensitivity, low in interference and simple in sample pretreatment, can be used for accurately measuring inorganic selenium, selenite and selenate as well as organic selenium, selenocysteine and selenomethionine and detecting selenium form in aquatic product.

The invention belongs to the technical field of chemical measurement methods and in particular relates to a method for measuring acrylic acid in air in working spaces by using ion chromatography. The rapid, sensitive, accurate, environment-friendly and low-cost method for measuring acrylic acid in air in working spaces by using ion chromatography is constructed by collecting the acrylic acid through a silicone tube and by taking environment-friendly pollution-free sodium carbonate/sodium bicarbonate as desorption solution and chromatographic mobile phase. A novel feasible way is provided for rapidly and accurately measuring acrylic acid in air in the working spaces, and the application of the ion chromatography in occupational health inspection is widened.

The invention relates to a on-line film abstractor combined with the ion chromatography, comprising three pumps, two six-way valve, a pre-column and a film abstractor. A trace amount of anion in the non-water-solubility organic matter is extracted to the deionized water through the hollow fiber film, the extract liquor is conveyed to the quantitative ring of the first six-way valve by the first liquid pump, then collected by the second liquid pump to the pre-column of the second six-way valve, the anion collected on the pre-column is transferred by the ion chromatographic pump to the ion chromatographic column for separating and detecting. The invention has few reagent consumption, direct sampling without the treatment, can detect the trace amount of anion in the non-water-solubility organic matter, thus enlarging the application range of ion chromatogram.

The invention provides a liquid chromatographic sampling passage cleaning device. The liquid chromatographic sampling passage cleaning device mainly comprises a microfluidic pump, a compressed air source, a capillary flow limiter, a liquid-gas switching valve, a sampling-cleaning switching valve, a waste liquid collecting cup, a waste liquid bottle, a sampling valve, a sampling port and a program controller. After sampling, a cleaning solution is used for reversely rinsing a liquid chromatographic sampling passage to reversely rinse particles left on a filter in the sampling process and discharge the particles out of a sampling pipeline first and then microflow gas is used for reversely purging the sampling passage to expel liquid left inside the pipeline so as to avoid sample dilution during sampling once again. The liquid chromatographic sampling passage cleaning device is suitable for various analytical-grade liquid chromatographs, comprising positive chromatography, reverse chromatography, ion chromatography and the like, as well as suitable for cleaning a sampling loop for flow injection analysis, so that cross pollution of samples is avoided, the sampling repeating precision is improved, and the service life of the sampling filter can be greatly prolonged.

The invention relates to a continuous separation and purification method for etimicin sulfate by using the technology of continuous ion chromatography to realize high efficient separation of etimicin sulfate. The method comprises that the hydrolysate of synthesized etimicin sulfate is fed into a continuous ion chromatography system, through the steps of adsorption, removal of impurities by washing, elution of the etimicin sulfate, collection of the eluent, and regeneration of the chromatographic columns; and the etimicin sulfate is obtained through nanofiltration concentration, vacuum condensation under reduced pressure, and salt formation and decolourization of the collected eluent. By using the method for separation and purification, the obtained etimicin are of high yield and purity, low cost, and environmental protection, suitable for industrial production.

A suppressor which comprises: an ion-exchange membrane; an eluate channel which is in contact with one side of the ion-exchange membrane, serves as a channel through which an eluate discharged from a separation column flows, and has inside no obstacle to the flow; a regenerant channel which is in contact with the other side of the ion-exchange membrane, serves as a channel through which a regenerant for regenerating ionic functional groups of the ion-exchange membrane flows, has been disposed so that the regenerant channel has no region facing the eluate channel and extends in parallel to the eluate channel in such a nearby position that the ionic functional groups can move through the ion-exchange membrane, and has inside no obstacle to the flow; and an ion-exchange membrane support member which is in contact at least with that region on one side of the ion-exchange membrane which is opposed to the regenerant channel and with that region on the other side of the ion-exchange membrane which is opposed to the eluate channel to thereby support the ion-exchange membrane with wall surfaces.

An apparatus for ion chromatography comprising a suppressor comprising a housing and a liquid conduit segment disposed in the housing, the liquid conduit segment including a membrane, the membrane having an inlet section adjacent the inlet of the conduit segment and an outlet section adjacent the outlet of the conduit segment, the inlet section having ion exchange sites capable of transmitting ions of one charge, positive or negative, and the outlet section being substantially non-retentive electrostatically for charged ionic species. Also, the method of using the apparatus.

The invention discloses a method for detecting Cr (VI) in cigarette paper by using an inhibition electrical conductivity detector characterized by ion chromatographic separation and large volume sample injection. The method comprises the specific steps as follows: shearing a cigarette paper sample into pieces, and accurately weighing cigarette paper pieces into a container; adding an extracting solution for vibrating extraction, and filtering the extracting solution by an aquo-system filter film, so as to obtain a to-be-detected solution; preparing a Cr (VI) standard solution, and testing and establishing a standard curve; and moreover, passing a filter liquor through an ion exchange column, and after isocratic elution, adopting the inhibition electrical conductivity detector and the ion chromatograph for analysis. According to the invention, as the large volume sample-injection electrical conductivity detector is adopted for detection, the derivation step is avoided, the preliminary treatment process is simplified, and the time, the labor and the materials for detection are saved; and further, the method can efficiently detect Cr (VI) in the cigarette paper, is rapid and reliable, is simple and convenient and easy to carry out, and fills the technical void in detecting Cr (VI) in the cigarette paper directly by the ion chromatography.

The invention provides a method for determining sulfur dioxide residual quantity in a Chinese herbal medicine, which comprises the following steps: using an acidification distillation method is used on a Chinese herbal medicine sample to be measured to prepare a tested solution; accurately absorbing the tested solution, using an ion chromatography, wherein a chromatographic column is IonPac AS-11-HC and a guard column is IonPac AG-11-HC, sampling, determining, calculating the sulfate radical content in the sample by using a standard curve method, and converting the content of sulfur dioxide in the sample. The method of the invention has the advantages of sensitivity, accuracy and convenience, and is suitable for detecting the sulfur dioxide residues in various Chinese herbal medicines.

The invention provides an ion chromatography detection method for simultaneous determination of sulfate radical and sulfite radical in desulfurized seawater. The invention optimizes the chromatographic conditions, through the solution combination of formaldehyde, sodium hydroxide, and ethylenediamine tetraacetic acid disodium salt of specific concentration, sample pretreatment can be achieved, and specific ion chromatography detection parameters are regulated to achieve baseline separation of sulfite radical and sulfate radical, i.e. a new sample pretreatment method is put forward, on the basis of realizing simultaneous accurate quantification of sulfate ions and sulfite ions, the influence of calcium and magnesium ions on the test can be removed. The result shows that the sulfate radical detection limit is 0.04mg/L, and the sulfite radical detection limit is 0.03mg/L. The method has the characteristics of rapid detection, high sensitivity and good accuracy, is suitable for simultaneous quantitative detection of sulfate and sulfite ions in a seawater desulfurization system, and also is suitable for detection of sulfate and sulfite single ions in water with calcium and magnesium cations.

The invention discloses a novel method for pretreating and measuring trivalent chromium ions and hexavalent chromium ions on line by an ion chromatographic column switching method. A system comprises a sample injector, a six-way valve, a reversed-phase column, a six-way valve and column switcher, a receiving pipe, a negative ion separation column, a four-source gradient pump, a liquid conveying pump, a three-way joint, a derivative reaction pipe, an ultraviolet visible spectrophotometer, a waste liquid bottle and the like. The problems that two ions are separated through a special separation column and are respectively detected through a double-wavelength ultraviolet visible detector at present are solved, and the dependency of detection on instrument equipment is reduced; meanwhile, due to the application of an on-line pretreatment system, water-soluble organic matters are removed from a sample, labor consumption is reduced, the time for sample pretreatment is saved, and the complicated operation process is avoided.

The method according to the present invention includes: a training sample measurement process (S1, S2) in which, for a food product belonging to the same kind as a determination target, a plurality of training samples individually labeled with a known state of quality are subjected to a measurement using a chromatograph mass spectrometer under the same analysis condition; a training sample data collection process (S3, S4) in which an index value related to the magnitude of a peak observed on an extracted ion chromatogram obtained by the measurement is acquired for each training sample, and the index value of the peak at each retention time common to the training samples is extracted; and a discrimination model creation process (S5-S7) in which a supervised training is performed to create a discrimination model, using, as the training data, the index value of the peak at each retention time common to the training samples acquired for each of the labeled training samples. Measurement data for an unknown sample is inputted into a discriminator based on the discrimination model, to obtain a quality discrimination result.