The invention discloses a method for simultaneously determining
sweetening agents and preservatives in tobacco essence, and belongs to the technical field of chemical component detection of auxiliary materials for tobaccos. The method comprises the following steps that 1, a sample to be determined is diluted, the pH value of a solution is regulated to be 9-10, extraction is performed, extraction liquid is purified through an Oasis HLB column, and the purified extraction liquid is filtered for standby application; 2, filtrate is taken to be subjected to
ion chromatographic analysis, and the content of the
sweetening agents and the preservatives in the sample to be determined is calculated by contrasting a
standard curve. According to the method, impurities disturbing determination in the essence are purified and removed in a classifying mode according to the physical and chemical properties of different types of the tobacco essence, one-time sample feeding is performed on the three
sweetening agents (
sodium cyclamate, acesulfame and
saccharin sodium) and the two preservatives (
sorbic acid and
benzoic acid) through an AS17C analytical column and an electrical
conductivity detector, and separation and determination are simultaneously performed. Compared with an industrially recommended determination method, the method has the advantages of being wide in
linear range, low in quantitation limit, little in sampling quantity, high in determination efficiency and the like.