6 results about "Simple sample" patented technology

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

The application discloses a method for quantitatively analyzing 21 arginine synthesis metabolites in mouse liver, kidney and serum based on liquid chromatography-mass spectrometry technology and application of the method in cantharidin hepatorenal toxicity research. The method comprises the following steps: preparing 21 metabolites and two internal standard solutions; optimizing mass spectrometry multiple reaction monitoring parameters; performing sample analysis after protein precipitation by acetonitrile, nitrogen blowing dry and 50% methanol water reconstitution; and establishing eight concentration level standard curves and adopting a weighted least square method to fit a linear equation. Methodology verification shows that the linear relationship of each metabolite is good (r 2 >0.99), and the precision, accuracy, matrix effect, recovery rate and stability all meet the requirements of biological sample analysis. The method has simple sample pretreatment, high sensitivity and strong specificity, is successfully applied to determination of the content of 21 arginine metabolites in a mouse hepatorenal toxicity model caused by cantharidin, and provides a reliable analysis tool for arginine metabolism research and cantharidin toxicity mechanism exploration.

The application belongs to the technical field of pharmaceutical analysis, and relates to a method for detecting residual acetic acid in a clilborol raw material by high performance liquid chromatography. An octadecylsilane bonded silica gel is used as a filling agent of a chromatographic column, 0.1% phosphoric acid aqueous solution is used as a mobile phase A, and acetonitrile is used as a mobile phase B to perform gradient elution. The detection method has the advantages of simple sample processing, strong specificity, good durability, and high detection sensitivity.

This invention provides a kit based on a three-phase enzyme-linked immunosorbent assay (ELISA) and its application. The construction of the three-phase ELISA sensor in the kit mainly includes the following steps: first, selecting a hydrophobic substrate, washing and pretreating it; second, immobilizing the coated antigen and oxidase on the substrate. This invention develops a competitive immunoassay method based on a three-phase substrate and applies it to the detection of 5-fluorouracil. This invention utilizes the specific immunoreaction between a collaboratively developed 5-fluorouracil monoclonal antibody and the antigen to quantitatively detect 5-fluorouracil, improving the selectivity for the target analyte. This immunosensor uses inexpensive equipment, is easy to operate, has high detection efficiency, simple sample processing, and exhibits a wide linear range and a high detection upper limit, making it easy to popularize and cost-effective, thus possessing high practical value.

The application discloses an electrochemical aptamer sensor for high-sensitivity detection of alpha-amatoxin and a preparation method and application thereof, and belongs to the technical field of electrochemical detection. The working electrode of the sensor is a glassy carbon electrode, gold nanocrystals are used to modify the electrode first, then aptamers are connected to the gold nanocrystals through gold-sulfur bonds, and the remaining active sites are closed by using mercaptohexanol. The electrochemical aptamer sensor has high selectivity and sensitivity for alpha-amatoxin, and has the advantages of simple sample pretreatment steps, rapidness and convenience. The detection result of an actual human sample is satisfactory. The electrochemical aptamer sensor prepared by the application has been successfully applied to the detection of alpha-amatoxin in human urine, and has the advantages of extremely low detection limit, wide detection range, excellent selectivity and stability.