43 results about "Feline calicivirus infection" patented technology

The invention discloses a multiplex PCR (polymerase chain reaction) primer group for simultaneously detecting feline panleucopenia virus, feline calicivirus and feline herpes virus type 1. The primer group comprises three pairs of primers, wherein the sequences of the primers for detecting the feline panleucopenia virus are shown in SEQ ID NO:1 and SEQ ID NO:2, the sequences of the primers for detecting the feline calicivirus are shown in SEQ ID NO:3 and SEQ ID NO:4, and the sequences of the primers for detecting the feline herpes virus type 1 are shown in SEQ ID NO:5 and SEQ ID NO:6. The feline panleucopenia virus, feline calicivirus and feline herpes virus type 1 can be simultaneously detected by using the primer sequences through a multiplex PCR method, the result is positive, while the detection result of other common feline viruses is negative, which shows that the primer group has the advantages of strong specificity, high sensitivity, good repeatability and the like.

The invention provides a primer, product and method for detecting feline respiratory tract pathogens as well as application, and relates to the technical field of nucleic acid tests. In the primer group for detecting the feline respiratory tract pathogens, a primer for detecting feline herpesvirus type 1 (FHV1), a primer for detecting feline calicivirus (FCV), a primer for detecting mycoplasma, aprimer for detecting feline chlamydia and a primer for detecting bordetella bronchiseptica are primers designed through highly conservative regions of each pathogen, have no cross reaction with otherfeline pathogens, and have the characteristics of high specificity and high sensitivity. By use of the primer group, five feline respiratory tract pathogens including the FHV1, the FCV, the mycoplasma, the feline chlamydia and the bordetella bronchiseptica can be identified in the same reaction system, and the detection cost and time are greatly reduced as compared with a traditional method.

The present invention relates to a novel, isolated and purified hemorrhagic feline calicivirus FCV-DD1. The invention further embraces monovalent and multivalent vaccines containing the new FCV-DD1 strain. In addition, the invention encompasses methods of protecting felines against infection or preventing disease caused by feline calicivirus alone or in addition to other pathogens that comprises administering to the felines an immunologically effective amount of the monovalent and multivalent vaccines described herein. Also, the invention concerns methods for diagnosing or detecting the hemorrhagic feline calicivirus in a susceptible host, asymptomatic carrier and the like by detecting the presence of feline calicivirus FCV-DD1 or antibodies raised or produced against feline calicivirus FCV-DD1 antigen.

The invention relates to a kit for detecting feline calicivirus and a detecting method thereof, in particular to an ELISA kit for detecting an IgG antibody of feline calicivirus and a detecting method thereof. The ELISA kit and the detecting method solve the problem that current feline calicivirus diagnosis and detection results are not ideal. The kit comprises an ELISA microwell plate, RaMIgG-HRP and substrate developing liquid. The method comprises the steps that firstly, serum is diluted and added into the ELISA microwell plate to be incubated; secondly, the RaMIgG-HRP is added for incubation; thirdly, the substrate developing liquid is added, and reaction is carried out in a shading mode at the room temperature; fourthly, a stop solution is added to terminate the reaction, an ELIASA is used for detecting 450 nm light absorption value, the 450 nm light absorption value and a Cut Off value of the ELISA kit are compared, and then a detection result is obtained. The ELISA kit detection result is high in repeatability and stability, and the speed is high.

The invention discloses a kit for simultaneously detecting multiple cat pathogens as well as a detection method and application of the kit. A specific primer group and a probe group are obtained through design, so that rapid and accurate detection and identification on feline plague viruses, feline calicivirus, feline infectious peritonitis viruses, feline toxoplasma gondii, feline herpes viruses, feline chlamydia and feline mycoplasma in one reaction are realized at the same time. The detection kit obtained on the basis of the design is high in specificity and sensitivity, the feline pathogens with the concentration of 1.7 * 10 <2> copies / mL can be effectively detected, repeated detection is not needed, the actual application accuracy is high, and the detection kit can be widely applied to epidemic situation monitoring, prevention and control of feline virus infection.

The present invention relates to improved methods and immunogenic and / or vaccine compositions for providing an immune response to feline calicivirus, including hypervirulent strains of feline calicivirus.

The present invention provides new feline calicivirus vaccines, including multivalent vaccines. The present invention also provides methods of making and using the vaccines.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

The invention discloses a multiplex PCR (polymerase chain reaction) primer group for simultaneously detecting feline panleucopenia virus, feline calicivirus and feline herpes virus type 1. The primer group comprises three pairs of primers, wherein the sequences of the primers for detecting the feline panleucopenia virus are shown in SEQ ID NO:1 and SEQ ID NO:2, the sequences of the primers for detecting the feline calicivirus are shown in SEQ ID NO:3 and SEQ ID NO:4, and the sequences of the primers for detecting the feline herpes virus type 1 are shown in SEQ ID NO:5 and SEQ ID NO:6. The feline panleucopenia virus, feline calicivirus and feline herpes virus type 1 can be simultaneously detected by using the primer sequences through a multiplex PCR method, the result is positive, while the detection result of other common feline viruses is negative, which shows that the primer group has the advantages of strong specificity, high sensitivity, good repeatability and the like.

The present invention provides a tandem expression of common antigenic epitopes of feline calicivirus GI and GII strain groups and the establishment of an indirect ELISA method. Using a prokaryotic expression vector, multiple B cell antigens of feline calicivirus VP1 protein were expressed in tandem. The expressed recombinant protein was purified and used as a coating antigen for the detection of feline calicivirus antibodies. A parallel comparison with the method of encapsulating whole virus showed that the value of detecting negative and positive sera was highly consistent. The antigen processing of the invention is convenient, the test time is shortened, and the operation steps are simpler. The invention establishes an indirect ELISA method for detecting the antibody level of feline calicivirus in cat serum, has the characteristics of good repeatability and high specificity, and can be used for feline calicivirus serological investigation. Therefore, the feline calicivirus indirect ELISA detection kit based on the tandem expression of the VP1 protein B cell antigen provided by the present invention is very suitable for the detection of large clinical samples, and is suitable for large-scale promotion.

The invention provides feline infectious rhinoconjunctivitis and feline panleukopenia dual vaccine and a preparation method thereof. The dual vaccine of the present invention uses a baculovirus / insect cell expression system to produce feline calicivirus virus-like particles and feline calicivirus respectively. The virus-like particles of feline panleukopenia virus are prepared by mixing the virus-like particles of the above two viruses with preservatives and adjuvants to prepare feline infectious rhinoconjunctivitis and feline panleukopenia dual vaccine. The double vaccine of the invention has the advantages of safety, high efficiency, easy production, low cost, etc., and has good immune prevention effect on feline infectious rhinoconjunctivitis and feline panleukopenia.