Feline calicivirus vaccine

A feline calicivirus, virus technology, applied in the direction of viruses, vaccines, viral peptides, etc., can solve the problems of lack of extensive cross-protection, no development, etc.

Pending Publication Date: 2022-06-07
INTERVET INT BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they all suffer from the problem identified above: lack of broad cross-protection
[0015] However, no live attenuated F

Method used

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  • Feline calicivirus vaccine
  • Feline calicivirus vaccine
  • Feline calicivirus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Neutralization indices for several FCV strains were determined using hyperimmune sera raised in cats against strains FCV F9 and Kalem Crouch. The experiment was performed as described in Section 8 below. The data are shown in Table 1. It becomes clear from the table that sera raised against Kalem Crouch have broad cross-protection against many other FCV strains. For sera against Kalem Crouch, significant Logs were seen against 16 out of 31 FCV strains 10 Decrease (ie > 1.5). Table 1 also shows that the cross-protection of the commonly used F9 strain is much lower. For 3 of the 22 FCV strains, sera raised against F9 showed significant Log 10 decrease (ie >1.5). It should be noted that the 2 FCV strains 3809, 6420, CV-21 that were neutralized or at least significantly reduced by F9 serum were F9-like viruses. Thus, not only does Kalem Crouch cross-protection for much more FCV strains than F9, but it also provides cross-protection for non-F9 strains.

[0118] Table ...

Embodiment 2

[0121] Construction of hybrid FCV-clones.

[0122] 1. cell culture

[0123] All cell lines were grown at 37°C, 5% CO 2 maintained in tissue culture flasks.

[0124] Cradell-Rees Feline Kidney (CrFK) cells were cultured in cells supplemented with 5% fetal bovine serum, 0.15% sodium bicarbonate, 2 mM L-glutamine, 100 U / ml penicillin, 10 μg / ml streptomycin, and 2 μg / ml amphotericin B grown in medium M6B8.

[0125] BsRT7 cells were maintained in medium DMEM supplemented with 5% fetal bovine serum, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1 mg / ml Geneticin (G418). Geneticin was removed at cell seeding prior to transfection.

[0126] 2. virus isolation

[0127] Oropharyngeal / nasal swabs were collected from cats and transported in medium M6B8. The swab was briefly vortexed and the virus suspension was inoculated onto confluent CrFK cells and incubated at 37°C and 5% CO. 2 Incubate until FCV-specific CPE is observed. Infected flasks were freeze-thawed to lyse cells, cla...

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Abstract

The invention relates to a feline calicivirus vaccine. The present invention relates to novel feline capsid proteins, live attenuated feline caliciviruses comprising the capsid proteins, live recombinant vector viruses and live attenuated hybrid feline caliciviruses comprising the capsid proteins, vaccines comprising such live attenuated feline caliciviruses, live recombinant vector viruses and live attenuated hybrid feline caliciviruses, and methods for preparing such viruses.

Description

[0001] This application is a divisional application of Chinese patent application 201680076354.1 "Feline Calicivirus Vaccine" with an application date of December 22, 2016. technical field [0002] The present invention relates to a novel feline calicivirus capsid protein, a live attenuated feline calicivirus comprising the capsid protein, a live recombinant vector virus comprising the capsid protein and a live attenuated hybrid feline calicivirus, including the Live attenuated feline calicivirus-like vaccines, live recombinant vector viruses and live attenuated hybrid feline caliciviruses, and methods for producing such viruses. Background technique [0003] Feline calicivirus (FCV) is a single-stranded positive-sense RNA virus belonging to the genus Vesivirus in the Caliciviridae family. The virus is highly infectious and causes upper respiratory disease (URD) ​​and mouth ulcers in cats. The virus is also associated with chronic gingivitis and stomatitis. More recently, ...

Claims

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Application Information

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IPC IPC(8): C07K14/08C12N7/02C12N7/04A61K39/12A61P31/14
CPCC07K14/005C12N7/00A61K39/12A61P31/14A61K2039/552A61K2039/5256A61K2039/5254C12N2710/16034C12N2770/16021C12N2770/16034C12N2770/16061C12N2770/32022C12N2770/32034A61P31/22A61P37/04Y02A50/30C07K14/08C12N7/02C12N7/04
Inventor E.舒胡A.S.鲍格
Owner INTERVET INT BV
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