The invention provides a recombinant virus of standard attack strain CVS-11 and a preparation method thereof. The invention establishes a reverse genetic manipulation system for rabies virus international standard attack strain CVS-11, provides an effective platform for further modifying or reconstructing the CVS-11 genome structure, and lays foundation for researching pathopoiesis mechanism, antigen site, virulence determined site and the like of rabies virus on the molecular level. On the basis of the CVS-11 reverse genetic manipulation system, the invention establishes a recombinant virus rCVS-11-eGFP for saving international standard attack strain CVS-11. The rCVS-11-eGFP expresses eGFP in the proliferation process, and can be used for directly observing the proliferation state and diffusion of the virus. By utilizing the characteristics of no toxicity, capability of self-luminescence without dependence on any accessory factor or substrate, and the like, the rCVS-11-eGFP is used as a detection antigen for determining the anti-rabies virus neutralizing antibody titer, and no operations, such as stain fixation and the like, are needed in the result determination process, so that the invention is economic and convenient.