16 results about "ENTEROBACTER HORMAECHEI" patented technology

The invention discloses enterobacter hormaechei XW-01 and a microbial preparation thereof, the strain (preservation number is CCTCC NO: M 20242595) can efficiently degrade protein, total sugar and nicotine in tobacco leaves, sensory indexes such as aroma and aroma quantity are remarkably improved, and protease activity is remarkably improved when the enterobacter hormaechei XW-01 and wheat bran act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco leaf treatment, through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like, the technical breakthrough that the protein content is reduced, the tar release amount is reduced, and the comprehensive quality grade is improved is achieved. Compared with a traditional method, the technology has the advantages that the treatment period is shortened, the cost is greatly reduced, the COD value of wastewater is greatly reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the method has a wide industrial application prospect.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The present application belongs to the field of microbial technology, and particularly relates to a bacteriophage preparation for controlling foodborne enterobacter and application thereof, wherein the bacteriophage preparation comprises any one or several of bacteriophage PhiEAS1, bacteriophage PhiEHO11, bacteriophage PhiEHO14 and bacteriophage PhiECL22. The four bacteriophages provided by the present application can be used alone or combined into a bacteriophage cocktail, and the influence of the bacteriophage preparation on enterobacter in different ecologies and environments is explored through bacteriophage cocktail therapy. The results show that the bacteriophage preparation exhibits significant bacteriostatic activity on various food substrates contaminated by Enterobacter hormaechei, such as lettuce, pork, milk, cherry tomato and the like; in an animal model, intraperitoneal injection of the bacteriophage preparation can effectively prevent and treat bacteremia caused by Enterobacter hormaechei, and shows potential as an anti-Enterobacter hormaechei biological control agent.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The invention provides a bacterium with polyethylene plastic degradation activity and application thereof, and belongs to the technical field of polyethylene plastic degradation. The bacterium with the activity of degrading the polyethylene plastic, provided by the invention, comprises at least one of pseudomonas putida ZSA123 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34958 and enterobacter hormaechei LBA140 with the preservation number of CGMCC No.34957. The bacterium provided by the invention can obviously reduce the load, tensile stress and fracture displacement rate of a polyethylene plastic film, and when the bacterium is used for degrading polyethylene plastic, a relatively high weight loss rate can be achieved while the degradation time is shortened, and the degradation rate of the bacterium on the polyethylene plastic is far higher than that of an existing strain in the field.

The present application relates to the field of microbial technology, and specifically to Enterobacter hormaechei C4 and its applications. Enterobacter hormaechei C4 is deposited at the General Microbiology Center of the China Culture Collection Administration, with the deposit address at No. 3, Yard 1, Beichen Road West, Chaoyang District, Beijing, on January 13, 2023, with the deposit number CGMCC No. 26480. The Enterobacter hormaechei C4 provided in the present application can utilize cellulose, starch, and other components in tobacco during the fermentation process to produce more flavor substances, thereby improving the aroma quality, aroma volume, concentration, stimulation, aftertaste, and sweetness of the tobacco, giving the tobacco better sensory quality.

The invention relates to the technical field of microorganisms, in particular to enterobacter hormaechei and application thereof. The strain is screened from rumen of cattle, cheap agricultural wastes such as corncobs and straws can be efficiently utilized as carbon sources for fermentation, the production cost of the xylanase is remarkably reduced, the yield of the xylanase is high, and the enzyme activity can reach 44.78 U / mL. Meanwhile, the xylanase produced by the strain has unique alkaline adaptability and still can keep more than 40% of activity in an alkaline environment with the pH value of 9.0, the catalytic activity of the xylanase can be effectively promoted by common additives such as Mn < 2 + > and Tween-80, the strain is suitable for production of the xylanase, and the application efficiency and economical efficiency of the strain are further improved.

The invention discloses enterobacter hormaechei Z2 and application thereof in improving soybean biomass, and belongs to the technical field of microorganisms. The collection number of the enterobacter hormaechei Z2 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.36028. The enterobacter hormaechei Z2 disclosed by the invention has relatively strong organophosphorus decomposing capability, and can promote the formation of a biological membrane and generate an iron carrier; and the soybean biomass is improved.

The invention provides a broad-spectrum compound bacterium system for harmlessly and efficiently degrading neonicotinoid pesticides and application of the broad-spectrum compound bacterium system. The broad-spectrum compound bacterial system provided by the invention comprises a bacterial strain Pantoea agglomerans, a bacterial strain Enterobacter hormaechei and a bacterial strain Raoultella ornitinolytica, and the broad-spectrum compound bacterial system provided by the invention comprises the following components: a bacterial strain Pantoea agglomerans, a bacterial strain Enterobacter hormaechei and a bacterial strain Raoultella ornitinolytica. A compound bacterial system formed by compounding the three strains can harmlessly and efficiently degrade different types of NNIs (acetamiprid, imidacloprid and thiamethoxam), and the compound bacterial system is fixed on porous coating sponge with a large specific surface area, so that efficient, stable and repeated utilization can be realized. The broad-spectrum compound bacterial system provided by the invention is simple in component and good in stability, has broad-spectrum characteristics, and can realize harmless degradation of different types of NNIs. By optimizing the assembly mode of the strains, the removal rate of the NNIs can be remarkably increased, and deep mineralization of the NNIs can be achieved.

The invention provides enterobacter hormaechei and application thereof. The preservation number of the Enterobacter hormaechei is CGMCC (China General Microbiological Culture Collection Center) NO. 33974, and the preservation number of the Enterobacter hormaechei is CGMCC NO. By adopting the enterobacter hormaechei disclosed by the invention, the conventional chemical components of cigar tobacco leaves are remarkably changed, the contents of total sugar, reducing sugar and total nitrogen are remarkably reduced, the total amount of aroma substances is remarkably increased and is 2.2 times that of aroma substances before aroma-producing bacteria are added, and the types of the aroma substances are increased, including acid, ketone, alcohol, hydrocarbon, aldehyde and furan compounds. The strain M9 has a remarkable effect of improving the aroma amount and the aroma quality of cigar tobacco leaves, and provides a theoretical basis and a technical support for application of a microbial aroma enhancement technology in the tobacco industry as a new microbial aroma enhancement resource.

The invention belongs to the technical field of tobacco microorganisms, and relates to a bacterial formula preparation for increasing the sweet aroma of tobacco fruits and softening smoke and a tobacco product, the bacterial formula preparation comprises bacillus altitudinis Y120, enterobacter hormaechei Y399 and bacillus velezensis YB371, and the bacillus altitudinis Y120, the enterobacter hormaechei Y399, the bacillus velezensis YB371, the bacillus velezensis YB371, the bacillus velezensis YB371, the bacillus velezensis YB371, the bacillus velezensis YB371 and the bacillus velezensis YB371 and the bacillus velezensis YB371 are used for increasing the sweet aroma of the tobacco fruits and softening the smoke. The preservation numbers of the strain in the China General Microbiological Culture Collection Center (CGMCC) are respectively CGMCC No.15626, CGMCC No.15627 and CGMCC No.30226. The invention also discloses a method for preparing the strain. The bacterial formula preparation is sprayed on the surface of a tobacco raw material, after fermentation, the fruit sweet fragrance of the tobacco raw material during combustion and smoking can be obviously improved, and smoke is softened.

The invention relates to a composite biological agent based on enterobacter hormaechei-bacillus subtilis, preparation of the composite biological agent and application of the composite biological agent to improvement of tobacco quality, and aims to solve the technical problems that cellulose in cigars is difficult to degrade and an effective microbiological method for improving the tobacco quality is lacked. According to the present invention, with the composite biological agent of the enterobacter hormaechei XW-01 strain with the preservation number of CCTCC NO: M 20242595, the bacillus subtilis MY-01 strain with the preservation number of CCTCC NO: M 20242596, and the metabolites thereof, the cellulose content in the tobacco leaf can be reduced, the irritation of the tobacco leaf during the burning and smoking process can be improved, and the overall quality and the smoking safety of the cigar can be improved; the two types of microorganisms cooperate to achieve a synergistic effect, and the composite biological agent prepared from the microorganisms has the technical advantages of being simple, convenient and efficient in technological process, low in tobacco leaf cost, short in period, safe, reliable, capable of obviously reducing the content of cellulose in tobacco leaves and improving the comprehensive quality of the tobacco leaves and the like, and has good application and popularization value.

The present invention comprises a consortium of Lactobacillus crispatus with pathogen resistance and anti-steatosis functions for nutraceutical and therapeutic uses and method thereof. The present invention comprises an indigenous microbiota that inhibit the growth of pathogenic bacteria, including Escherichia coli, Klebsiella pneumoniae, Enterobacter hormaechei, Staphylococcus aureus, Gardnerella vaginalis and reduce the accumulation of visceral and hepatic fat.

The invention discloses a composite biological agent based on enterobacter hormaechei-bacillus megaterium, preparation of the composite biological agent and application of the composite biological agent in tobacco quality improvement, and aims to solve the technical problems that cellulose and starch in cigars are difficult to degrade, and an effective microbiological method for improving the tobacco quality is lacked. According to the present invention, with the composite biological agent of the enterobacter hormaechei XW-01 strain with the preservation number of CCTCC NO: M20242595, the bacillus megaterium XW-02 strain with the preservation number of CCTCC NO: M2025185, and the metabolites thereof, the cellulose content and the starch content in the tobacco leaf can be reduced, the irritation of the tobacco leaf during the burning and smoking process can be improved, and the overall quality and the smoking safety of the cigar can be improved; the two types of microorganisms cooperate to achieve a synergistic effect, and the composite biological agent prepared from the microorganisms has the technical advantages of being simple, convenient and efficient in technological process, low in tobacco leaf cost, short in period, safe, reliable, capable of obviously reducing the content of cellulose and starch in tobacco leaves and improving the comprehensive quality of the tobacco leaves and the like, and has good application and popularization value.

The invention relates to application of enterobacter hormaechei XW-01 in tobacco quality improvement. The XW-01 and a preparation thereof can be used for reducing the content of cellulose, total sugar and nicotine in cigars, increasing the tension value, improving the sensory quality (such as irritation, offensive odor and the like) of tobacco stems and improving the quality of cigarettes. The preparation method of the microbial preparation comprises the steps of strain activation, seed solution preparation, amplification culture, microbial agent preparation and the like. In addition, the invention further discloses a tobacco leaf processing method which comprises the steps of adjusting the water content of the tobacco leaves, spraying the microbial preparation, then sealing and fermenting, drying, balancing the water and the like. The tobacco leaf treatment cost is low, the treatment period is short, safety and reliability are achieved, the cellulose content in the tobacco leaves can be effectively reduced, the tobacco leaf quality is effectively improved, and the wide and practical significance is achieved for improving the cigarette quality and increasing the economic benefits of enterprises.

This invention discloses the application of *Enterobacter holmieae* in the synthesis of dihydroouabain, belonging to the field of microbial technology. The dihydroouabain synthesized by *Enterobacter holmieae* in this invention, due to its unique biological characteristics, has broad application prospects in cardiovascular disease treatment, neuroscience research, energy metabolism, and circadian rhythm regulation. The *Enterobacter holmieae* strain described in this invention is preserved under the name *Enterobacter holmieae*. Enterobacter hormaechei Deposited at Guangdong Provincial Center for Microbial Culture Collection, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou; Deposit date: April 16, 2026; Accession number: GDMCC No:68091.