The present application belongs to the field of microbial technology, and particularly relates to a
bacteriophage preparation for controlling foodborne
enterobacter and application thereof, wherein the
bacteriophage preparation comprises any one or several of
bacteriophage PhiEAS1, bacteriophage PhiEHO11, bacteriophage PhiEHO14 and bacteriophage PhiECL22. The four bacteriophages provided by the present application can be used alone or combined into a bacteriophage cocktail, and the influence of the bacteriophage preparation on
enterobacter in different ecologies and environments is explored through bacteriophage cocktail therapy. The results show that the bacteriophage preparation exhibits significant bacteriostatic activity on various food substrates contaminated by
Enterobacter hormaechei, such as lettuce, pork, milk,
cherry tomato and the like; in an
animal model,
intraperitoneal injection of the bacteriophage preparation can effectively prevent and treat
bacteremia caused by
Enterobacter hormaechei, and shows potential as an anti-
Enterobacter hormaechei biological control agent.