34 results about "ENTEROBACTER HORMAECHEI" patented technology

The invention discloses a preparation method of a nano-silver composite antibacterial agent. The invention is characterized in that the nano-silver composite antibacterial agent is formed by combining a nano-silver solution and an organic antibacterial agent which have synergistic effect and adduction effect according to a certain ratio. The structure and performance of the antibacterial agent after combination are stable, and the antibacterial agent has more rapid, efficient and broad-spectrum antibacterial performance. The minimal inhibitory concentration (MIC) of the antibacterial agent for inhibiting escherichia coli, Enterobacter hormaechei, staphylococcus aureus, bacillus subtilis, Rhodotorula mucilaginosa, penicillium and Aspergillus niger is only 1-10 microgram / ml. The preparation method of the nano-silver composite antibacterial agent is characterized in that the nano-silver in the nano-silver composite antibacterial agent has monodispersity and there is no agglomeration phenomenon. The preparation method has advantages of ingenious conception, simple experimental operation, high operationality and short period, and is suitable for large-scale production.

The invention discloses a deamination and deodorization bacterial strain QDN01 and an application thereof in biological deodorization. A deamination and deodorization bacterial strain is screened out from grass root black soil, and the biological characteristics and the denitrification condition on chicken manure of the bacterial strain are researched. By analysis of physio-biochemical characteristics and a 16SrDNA sequence, the deamination and deodorization bacterial strain is identified as enterobacter hormaechei, is named Enterobacter hormaechei QDN01, and is preserved in the China Center for Type Culture Collection in Wuhan University with the preservation number of CCTCC NO:M2013670. The research shows that the separated bacterial strain QDN01 is stably in biological characteristics, and fast in reproductive capacity, and particularly can quickly grow in the chicken manure to which starch is added, the increment is up to 250000000000cfu / ml, and meanwhile, a good denitrification and deodorization effect can be achieved. Compared with the prior art, the beamination and deodorization bacterial strain has obvious advantages. Therefore, a feasible and practical technical scheme is provided for innocent biological treatment of poultry manure, and the deamination and deodorization bacterial strain has a good development prospect.

The invention relates to a lignocellulose substance efficient degradation bacterium K24 and an application thereof. The lignocellulose substance efficient degradation bacterium K24 is enterobacter hormaechei K24 and is collected in the China General Microbiological Culture Collection Center (CGMCC) on December 12, 2014, with collection number of CGMCC No.10169. The lignocellulose substance efficient degradation bacterium K24 is used for degrading lignocellulose substances. The lignocellulose substance efficient degradation bacterium K24 has the beneficial effects that the strain is rapid in proliferation, has strong adaptability and is widely applied; the weight loss ratios of waste agaric material substitutes subjected to fermentation treatment with enterobacter hormaechei K24 are more than 39%; and the weight loss ratios of corn straws subjected to fermentation treatment with enterobacter hormaechei K24 are more than 29%.

The invention discloses a biological surfactant produced by Enterobacter hormaechei and applications thereof. Enterobacter hormaechei is used to produce the biological surfactant. The formula and the production conditions are deeply explored. The addition amounts of carbon source and nitrogen source are improved, the production and fermentation conditions are improved, and thus the yield of surfactant can reach more than 2 grams for each liter. The biological surfactant and waste livestock and poultry amino acid hydrolysate are innovatively compounded, the nutrient loss is avoided, and the purification treatment is not needed.

The invention discloses a bacterial preparation for increasing oral sweetness and improving oral comfort during combustion and smoking of tobacco, and a tobacco product. The bacterial preparation comprises bacillus velezensis YBN3, bacillus altitudinis Y120 and enterobacter hormaechei Y399, wherein the accession numbers of the above three bacterial strains in the China General Microbiological Culture Collection Center are respectively CGMCC No. 15625, CGMCC No. 15626 and CGMCC No. 15627. The bacterial preparation disclosed by the invention is sprayed on the surface of a tobacco raw material; and after fermentation, the oral sweetness of the tobacco raw material during combustion and smoking can be obviously increased and the oral comfort of the tobacco raw material is integrally improved.

The invention relates to enterobacter hormaechei CL2013 separated from chromium slag storage yard soil, the strain of which is preserved on January 27, 2015, and the preservation number is CGMCC NO.10451. The strain is cultured on an LB culture medium, and the cell is in a rod shape and is Gram-negative; the stain is cultured for 3 days, bacteria colony is in milky white color, the shape of the bacteria colony is circular and smooth, and the strain is determined as the enterobacter hormaechei by virtue of physiological and biochemical reaction and molecular biology. The enterobacter hormaechei CL2013 has good effect for reducing the hexavalent chromium. The invention also discloses a method for preparing hexavalent chromium repairing bactericide by utilizing the enterobacter hormaechei CL2013 strain and marigold processing waste liquid and waste slag. The method is simple, low in cost, free from secondary pollution, convenient to use and wide in market prospect.

The invention discloses a microbial flora for treating high-salt industrial wastewater and aims to screen and compound functional florae and verify the application effects on the basis of the characteristics that high-salt wastewater is high in salt content, high in chromaticity, high in COD, low in BOD and poor in biochemical property and contains complicated contaminants. Through isolation, screening and acclimatization, an active flora is obtained, the active flora has the capability of tolerating 18% high-salt wastewater and can be applied to biochemical treatment on high-salt pickling wastewater; according to the components and the proportion of the flora, relevant strains are purchased from the China General Microbiological Culture Collection Center and used for simulating the flora components of the acclimatized microbial flora, that is, enterobacter hormaechei, bacillusmegatherium, chromohalobacterjaponicas, halomonasxianhensis, chryseobacteriumindoltheticum and clostridiumsporogenes are compounded in a quantity ratio of 2:2:1:1:1:1, a relatively remarkable degradation effect is achieved when the compounded microbial flora is used for treating wastewater, COD in high-salt wastewater can be degraded to 1935.62mg / L from 29465mg / L, the COD degradation rate is 93.43%, and an effective technical support is provided for biological treatment on high-salt industrial wastewater which is hard to treat.

The invention belongs to the field of bioengineering and relates to a preparation method and application of a carotenoid 9,10' dioxygenase. The preparation method comprises the following steps: cultivating enterobacter hormaechei, and extracting a whole genome sequence; constructing a recombinant plasmid of a carotenoid 9,10' dioxygenase gene; inducing expression of the carotenoid 9,10'dioxygenase gene, and purifying zymoprotein; and analyzing and identifying enzymatic properties of the carotenoid 9,10' dioxygenase. The prepared carotenoid 9,10' dioxygenase gene can efficiently catalyze and synthesize beta-ionone, catalysis specificity is high, reaction products are few, and reaction time is short; a novel enzyme resource different from discovered carotenoid 9,10' dioxygenase is obtained; the carotenoid 9,10' dioxygenase has different amino acid sequences and enzymatic characteristics; and reaction conditions are optimized, and yield of the beta-ionone reaches 79.4%.

The invention relates to Enterobacter hormaechei L124 and application thereof to decomposition of agricultural wastes. The Enterobacter hormaechei L124 is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 10164. The Enterobacter hormaechei L124, provided by the invention, has the advantages of rapid bacterial strain proliferation, high adaptability and wide application; the weight loss ratio of bag planted edible fungus waste fermented by the Enterobacter hormaechei L124 is 30% or above; and the weight loss ratio of a maize straw fermented by the Enterobacter hormaechei L124 is 28% or above.

The invention discloses an alga-lysing microbial agent and application thereof. The microbial agent contains enterobacter and / or an enterobacter extract, and the enterobacter is selected from enterobacter hormaechei F2 or enterobacter cloacae. The microbial algicidal inoculant contains the enterobacter hormaechei F2 and / or the enterobacter hormaechei F2 extract, and has an obvious dissolution effect on microcystis aeruginosa in a water body, the algicidal rate reaches 95%, the algicidal rate on filamentous algae reaches 97%, the removal rate of algal cells in an actual eutrophic water body can reach 87.32%, and the removal rate of the microcystis aeruginosa in the actual eutrophic water body can reach 87.32%. The alga-lysing microbial agent has great application in water eutrophication treatment.

Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g / L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane / tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.

The invention discloses enterobacter hormaechei bacteriophage YZU.P.A-5 and application thereof. The bacteriophage is identified to have unique genome structure characteristics, and belongs to a novelkind of bacteriophage; and the bacteriophage has been preserved in China Center for Type Culture Collection since June 15, 2020 under the preservation number of CCTCC NO: M 2020206. The bacteriophagedisclosed by the invention can efficiently crack enterobacter hormaechei so as to effectively inhibit enterobacter hormaechei in various matrices; and thus, the bacteriophage can be applied to preparation of a bacterial depressant for controlling enterobacter hormaechei pollution. The bacterial depressant prepared by the bacteriophage can effectively control growth of enterobacter hormaechei in culture media and food matrices, as well as effectively inhibit formation of enterobacter hormaechei mycoderm on surfaces of equipment, utensils and raw material solids; and moreover, the bacterial depressant is simple to prepare and convenient to use, as well as capable of reducing risks of enterobacter hormaechei propagation and food-borne diseases.

The invention relates to an application of enterobacter hormaechei K20 to degrading agricultural wastes. The enterobacter hormaechei ( Enterobacter hormaechei ) K20 is preserved in China General Microbiological Culture Collection Center, at 12 December 2014, wherein the preservation number is CGMCC No.10163. Bacterial strains are proliferated, the adaptability is high, and the application is broad. The weight loss rate of bagged fungus waste after fermentation treatment with the enterobacter hormaechei ( Enterobacter hormaechei ) K20 is 23% or above, and the weight loss rate of corn straw after fermentation treatment with the enterobacter hormaechei ( Enterobacter hormaechei ) K20 is 27% or above.

The invention discloses a preparation method of a nano-silver composite antibacterial agent. The invention is characterized in that the nano-silver composite antibacterial agent is formed by combining a nano-silver solution and an organic antibacterial agent which have synergistic effect and adduction effect according to a certain ratio. The structure and performance of the antibacterial agent after combination are stable, and the antibacterial agent has more rapid, efficient and broad-spectrum antibacterial performance. The minimal inhibitory concentration (MIC) of the antibacterial agent for inhibiting escherichia coli, Enterobacter hormaechei, staphylococcus aureus, bacillus subtilis, Rhodotorula mucilaginosa, penicillium and Aspergillus niger is only 1-10 microgram / ml. The preparation method of the nano-silver composite antibacterial agent is characterized in that the nano-silver in the nano-silver composite antibacterial agent has monodispersity and there is no agglomeration phenomenon. The preparation method has advantages of ingenious conception, simple experimental operation, high operationality and short period, and is suitable for large-scale production.

The invention discloses enterobacter hormaechei and application thereof in production of vanillin, and belongs to the technical field of biology, the enterobacter hormaechei is named as DQ and preserved in the China General Microbiological Culture Collection Center on July 26, 2021, and the preservation number is CGMCC No.22958. The enterobacter hormaechei (DQ) can effectively utilize trans-ferulic acid to produce vanillin, the yield and the utilization efficiency of vanillin can be greatly improved, and the strain has good ferulic acid and vanillin tolerance, so that the production and economic benefits are improved, the production in a special environment is met, the defects that the fermentation period is long, the condition universality is poor, the strain conversion rate is low, high-concentration ferulic acid is not resisted and the like when the vanillin is produced by fermentation of modern microorganisms by taking trans-ferulic acid as a substrate are effectively overcome.

The invention belongs to the technical field of biological hydrogen production, and particularly relates to a preparation method of a biomass photosynthetic hydrogen production mixed flora, and the method comprises the following preparation steps of: S1, preparing a bacterial growth culture medium; S2, amplifying a bacterial liquid; according to the novel photosynthetic bacteria, HAU-M1 photosynthetic bacteria and enterobacter hormaechei are used as co-culture hydrogen-producing bacteria, a growth medium and the co-culture hydrogen-producing bacteria are added into an anaerobic sealed culture container, and the mixture is placed under proper conditions for culture and amplification; and S3, conducting hydrogen production experiment. Enterobacter hormaechei is introduced into the HAU-M1 photosynthetic bacterium flora, the prepared photosynthetic bacterium mixed flora can secrete a rhamnolipid surfactant in the culture process, the hydrogen production amount of a light fermentation biological hydrogen production system is effectively increased, and the effect is obvious; when 6% of enterobacter hormaechei is added, the accumulated hydrogen production of light fermentation reaches a peak value and is far greater than that of a group without the enterobacter hormaechei; the method has the advantages of low cost, high efficiency, environmental friendliness, strong adaptability and the like, and has wide application prospects in the field of light fermentation biological hydrogen production.

The invention discloses a microbial flora for treating high-salt industrial wastewater and aims to screen and compound functional florae and verify the application effects on the basis of the characteristics that high-salt wastewater is high in salt content, high in chromaticity, high in COD, low in BOD and poor in biochemical property and contains complicated contaminants. Through isolation, screening and acclimatization, an active flora is obtained, the active flora has the capability of tolerating 18% high-salt wastewater and can be applied to biochemical treatment on high-salt pickling wastewater; according to the components and the proportion of the flora, relevant strains are purchased from the China General Microbiological Culture Collection Center and used for simulating the flora components of the acclimatized microbial flora, that is, enterobacter hormaechei, bacillusmegatherium, chromohalobacterjaponicas, halomonasxianhensis, chryseobacteriumindoltheticum and clostridiumsporogenes are compounded in a quantity ratio of 2:2:1:1:1:1, a relatively remarkable degradation effect is achieved when the compounded microbial flora is used for treating wastewater, COD in high-salt wastewater can be degraded to 1935.62mg / L from 29465mg / L, the COD degradation rate is 93.43%, and an effective technical support is provided for biological treatment on high-salt industrial wastewater which is hard to treat.

The invention discloses application of a combination of PQS and enterobacter hormaechei in inhibition of growth and reproduction of algae. The algicidal microbial inoculum comprises enterobacter hormaechei and PQS, and the enterobacter hormaechei is enterobacter hormaechei F2. The PQS in the algicidal bacterial agent does not have the functions of algicidal and algal inhibition, the algicidal effect of the enterobacter hormaechei F2 can be improved through the synergistic effect of the PQS and the enterobacter hormaechei F2, the algicidal rate reaches up to 83.81%, in addition, the content of chlorophyll a in an algicidal solution can be remarkably reduced while the biomass of the enterobacter hormaechei F2 is not affected through the addition of the PQS, and the content of chlorophyll a in the algicidal solution can be remarkably reduced. The activity of ROS (reactive oxygen species) in algae liquid can be remarkably improved, so that the flooding of algal bloom can be effectively controlled, and the method has a relatively great application prospect in the treatment of eutrophication of a water body.

The invention discloses a method for production of 8-methyl-alpha-ionone by biodegradation of lutein. The method utilizes Enterobacter hormaechei for degradation of lutein to generate 8-methyl-alpha-ionone. The method provided by the invention employs Enterobacter hormaechei for the first time to degrade lutein so as to generate the aromatic substance 8-methyl-alpha-ionone, opens up a new way for production of aromatic substances through biodegradation of lutein, solves the technical problems of difficult separation, many reagents and environmental burden in the traditional method of physical-chemical degradation of lutein to generate aromatic substances, and has the characteristics of mild reaction conditions, easy separation, green and environmental protection, etc. By comparing the degradation effects of different substrate concentrations, the invention finds that an optimal lutein degradation effect can be achieved when the concentration of in a fermentation medium is 400-700mg / L.

The invention belongs to the field of bioengineering and relates to a preparation method and application of a carotenoid 9,10' dioxygenase. The preparation method comprises the following steps: cultivating enterobacter hormaechei, and extracting a whole genome sequence; constructing a recombinant plasmid of a carotenoid 9,10' dioxygenase gene; inducing expression of the carotenoid 9,10'dioxygenase gene, and purifying zymoprotein; and analyzing and identifying enzymatic properties of the carotenoid 9,10' dioxygenase. The prepared carotenoid 9,10' dioxygenase gene can efficiently catalyze and synthesize beta-ionone, catalysis specificity is high, reaction products are few, and reaction time is short; a novel enzyme resource different from discovered carotenoid 9,10' dioxygenase is obtained; the carotenoid 9,10' dioxygenase has different amino acid sequences and enzymatic characteristics; and reaction conditions are optimized, and yield of the beta-ionone reaches 79.4%.

The invention discloses enterobacter hormaechei and application of enterobacter hormaechei in preparing antibacterial drugs. The preservation number of enterobacter hormaechei No. 5 is CCTCC No. M2013695. The enterobacter hormaechei No. 5 has antagonism to multiple bacteria, so that the enterobacter hormaechei No. 5 can be used for preparing preparation for preventing and controlling vibrio alginolyticus or preparing assistant hemolysis vibrio preparation; the preparation for preventing and controlling vibrio alginolyticus or the assistant hemolysis vibrio preparation can be used for beneficial microbial preparation to replace chemical medicine, improves water quality, and eliminates vibrio alginolyticus or assistant hemolysis vibrio in an aquatic product cultivation system, so that damage to aquatic product cultivation objects from the vibrio alginolyticus or assistant hemolysis vibrio is prevented, the development of the aquatic product cultivation industry is promoted, aquatic products are prevented from carrying the vibrio alginolyticus or assistant hemolysis vibrio, and health and safety of human beings are ensured.

The invention relates to a high-value utilization method of cake meal capable of preventing aflatoxin infection, and belongs to the field of high-value utilization of plant cake meal. According to the method disclosed by the invention, the cake meal obtained by pressing oil from plants is used as a raw material. The processing method comprises the following steps of soaking of the meal cake in Chinese herbal medicines and water, enzymolysis, fermentation, grinding into thick liquid, homogenizing, spray drying and the like. In the method, when the cake meal is soaked in the Chinese herbal medicines and the water, phellodendron, cloves, smoked plums, herba artemisiae scopariae, rhizoma coptidis, figwortflower picrorhiza rhizomes, Chinese pulsatilla roots and the like are added; pancreases are used for the enzymolysis; bacillus licheniformis and enterobacter hormaechei are used for microbial fermentation. Cake meal powder processed by the method is high in price, good in protein solubility, high in absorption and utilization degrees and free from aflatoxin pollution; the cake meal as a feed can effectively prevent and treat various breeding diseases and insect pests, bred animals grow fast, economic benefits and social benefits are notable, and high-value utilization of the plant cake meal is realized.

The invention relates to a lignocellulos substance high-efficiency degrading bacterium K20 and application thereof. The lignocellulos substance high-efficiency degrading bacterium K20 is Enterobacter hormaechei K20 and was preserved with preservation number CGMCC No.10163 on December 12, 2014 by China General Microbiological Culture Collection Center. The bacterium is used for degrading lignocellulos substances. The strain provided by the invention has the advantages of quick proliferation, strong applicability and wide application; a weight-loss ratio of bagged agaric wastes after being fermented by Enterobacter hormaechei K20 is above 23 percent and a weight-loss ratio of corn stalks after being fermented by Enterobacter hormaechei K20 is above 27 percent.

Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g / L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane / tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.

A strain of Enterobacter hormaechei CL.2013 isolated and screened from the soil of the chromium slag yard was preserved on January 27, 2015, with the preservation number CGMCC No.10451. The strain was cultured on LB medium, the cell shape was rod-shaped, and Gram staining was negative; after 3 days of culture, the color of the colony was milky white, and the shape of the colony was round and smooth. The strain was confirmed by physiological and biochemical reactions and molecular biological identification Enterobacter hormaechei. Enterobacter hallii CL.2013 strain has a strong reducing effect on hexavalent chromium. The invention also discloses a method for preparing a hexavalent chromium restoration bacterial agent by using Enterobacter hallii CL.2013 strain and marigold processing waste liquid and waste residue. The method is simple, low in cost, has no secondary pollution, is convenient to use and has broad market expectation.

The invention relates to a lignocellulose substance efficient degradation bacterium K24 and an application thereof. The lignocellulose substance efficient degradation bacterium K24 is enterobacter hormaechei K24 and is collected in the China General Microbiological Culture Collection Center (CGMCC) on December 12, 2014, with collection number of CGMCC No.10169. The lignocellulose substance efficient degradation bacterium K24 is used for degrading lignocellulose substances. The lignocellulose substance efficient degradation bacterium K24 has the beneficial effects that the strain is rapid in proliferation, has strong adaptability and is widely applied; the weight loss ratios of waste agaric material substitutes subjected to fermentation treatment with enterobacter hormaechei K24 are more than 39%; and the weight loss ratios of corn straws subjected to fermentation treatment with enterobacter hormaechei K24 are more than 29%.

The invention discloses a method for producing pimelic acid through fermentation of enterobacter hormaechei, and belongs to the technical field of microbial fermentation. The enterobacter hormaechei capable of producing pimelic acid is screened from preserved strains, the strain is a natural strain, antibiotics and inducers do not need to be added in the fermentation process, the industrial production cost is reduced, and meanwhile environment protection is facilitated. When the enterobacter hormaechei is fermented for 72 hours under a shake flask condition, the yield of pimelic acid is 115.4 mg / L; by optimizing the fermentation processes such as the carbon source type, the nitrogen source type, the carbon and nitrogen source combination, the inoculum size, the type and concentration of additional inorganic salt and the like, the yield of pimelic acid is further increased and reaches 217.3 mg / L. Meanwhile, a new host is provided for biological synthesis of pimelic acid, and a biological foundation is also laid for subsequent research.

The invention discloses enterobacter hormaechei and application of enterobacter hormaechei in preparing antibacterial drugs. The preservation number of enterobacter hormaechei No. 5 is CCTCC No. M2013695. The enterobacter hormaechei No. 5 has antagonism to multiple bacteria, so that the enterobacter hormaechei No. 5 can be used for preparing preparation for preventing and controlling vibrio alginolyticus or preparing assistant hemolysis vibrio preparation; the preparation for preventing and controlling vibrio alginolyticus or the assistant hemolysis vibrio preparation can be used for beneficial microbial preparation to replace chemical medicine, improves water quality, and eliminates vibrio alginolyticus or assistant hemolysis vibrio in an aquatic product cultivation system, so that damage to aquatic product cultivation objects from the vibrio alginolyticus or assistant hemolysis vibrio is prevented, the development of the aquatic product cultivation industry is promoted, aquatic products are prevented from carrying the vibrio alginolyticus or assistant hemolysis vibrio, and health and safety of human beings are ensured.