6 results about "Nicotine degradation" patented technology

The present application relates to a kind of high-purity nicotine preparation method derived from tobacco stem, belong to plant extraction technical field.This method is for the large amount of tobacco stem waste produced in the process of tobacco planting and baking, proposes an efficient nicotine extraction method.By crushing tobacco stem, using acid aqueous solution for acidification treatment, then using alkaline solution to adjust pH value and carry out steam distillation, collect distillate.Subsequently, using reverse osmosis membrane concentration and molecular distillation technology for purification, obtain high-purity nicotine solution.This method does not use organic solvent, reduces the security risk, at the same time improves the extraction rate and purity of nicotine, purity can reach 95%~99%, extraction efficiency is more than 85%.In addition, the method uses membrane distillation process to reduce energy consumption, reduce the loss of active ingredients, and further remove impurities through molecular distillation technology, prevent nicotine degradation and oxidation deterioration.The implementation of the present application not only realizes the high value-added utilization of tobacco stem, but also improves the economic benefit, reduces the pollution to the environment.

The invention provides a nicotine degrading enzyme mutant as well as a preparation method and application thereof. The invention provides a recombinant nicotine degrading enzyme mutant. On the basis of NicA2 with an amino acid sequence as shown in SEQ ID NO: 1, mutation is carried out at any one of the following positions: the 48th position, the 379th position, the 189th position, the 208th position, the 234th position, the 274th position, the 317th position, the 369th position, the 370th position, the 362th position, the 396th position, the 462th position and the 479th position. Compared with a wild type nicotine degrading enzyme, the mutant has the advantages that the nicotine degrading activity is obviously improved, and the mutant has higher application potential in the aspects of further preparing a medicine for treating nicotine addiction or nicotine poisoning and improving the nicotine degrading activity and stability.

The invention relates to preparation of a spore-containing enteric targeted release microsphere and application of the spore-containing enteric targeted release microsphere in nicotine degradation. A probiotic bacillus subtilis 168 strain is subjected to engineering modification, so that a nicotine degrading enzyme is displayed on the surface of a spore of the probiotic bacillus subtilis 168 strain. In order to realize targeted release of intestinal tracts and reduce loss of recombinant spores in a gastric acid environment, the recombinant spores are wrapped by natural polymeric materials sodium hyaluronate and sodium alginate with pH responsiveness, and the spore microspheres with enteric targeted release are prepared. The microspheres greatly protect recombinant spores, effectively realize'interception type degradation 'of nicotine before nicotine is absorbed into blood by intestinal tracts, and continuously degrade nicotine diffused from blood by a'metabolic siphon' effect, so that the concentration of nicotine combined with a central nicotine receptor is remarkably reduced; brain dopamine reward effect induced by smoking is weakened fundamentally, positive feedback circulation of smoking-pleasant sensation is broken effectively, and visceral organs are protected from toxic and side injury of nicotine.

The invention belongs to the field of development and utilization of tobacco stem waste, and particularly relates to a tobacco stem treatment method capable of retaining nicotine and enhancing exposure and application. The method comprises the following steps: firstly, carrying out chemical pretreatment on tobacco stem particles: adjusting the pH value with a buffer solution, replacing with specific resin, and keeping nicotine stable under a mild condition; and performing mechanical treatment by splitting or ball milling, and intermittently controlling the temperature to prevent nicotine degradation, thereby obtaining the target product. Detection shows that the microstructure of the treated tobacco stem is fully damaged, the nicotine exposure rate and the dissolution concentration are remarkably improved, and the tobacco stem has a good antibacterial effect on staphylococcus aureus and salmonella. The nicotine can be efficiently extracted or directly used for bacteriostasis, high-value utilization of tobacco stalk waste is achieved, and environment friendliness and economical efficiency are both achieved.

This invention pertains to genetic engineering and relates to an orally administered engineered microbial system and its applications. Through genetic manipulation techniques such as genome integration, a dominant mutant of the nicotine-degrading enzyme Nox is displayed on the surface of Bacillus subtilis spores. This mutant is then coupled with catalase or peroxidase, and genes related to spore germination and the system's own recombinant enzymes are knocked out to construct a strain with self-eliminating resistance genes. This invention utilizes orally administered engineered bacteria to colonize the intestines. The "molecular degradation machine and metabolic siphon" effect produced by the engineered bacteria continuously degrades nicotine diffused from the bloodstream, thereby significantly reducing the concentration of nicotine binding to central nicotine receptors. This fundamentally weakens the dopamine reward effect induced by smoking, effectively breaking the positive feedback loop of "smoking-pleasure," and providing a new method for treating nicotine dependence. As a "plug-and-play" platform technology, displaying different degradation enzymes on the surface of Bacillus subtilis spores can also degrade other harmful substances.

The application discloses an oral composition, a mouth product, a drink and related applications of the oral composition. The oral composition provided by the application comprises a nicotine dehydrogenase, probiotics and food-grade synergistic fiber, the nicotine dehydrogenase is derived from Pseudomonas putida, and the probiotics are Lactobacillus plantarum. The oral composition provided by the application has a nicotine degradation rate of 90.5% in a simulation system. Meanwhile, the composition has excellent processability, gastrointestinal stability, high food compatibility and bioavailability.