41 results about "Nicotine degradation" patented technology

The invention relates to a technology of biological degradation of nicotine in tobacco, in particular to a pseudomonad ZCJ bacterial strain applied to nicotine degradation of tobacco and a screening method and an application thereof. The pseudomonad ZCJ bacterial strain (Pseudomonas sp. ZCJ) was preserved in the China Center for Type Culture Collection on March, 30th, 2009 with a preservation number of CCTCC NO. M209064. The invention also discloses screening and application of the bacterial strain. In the invention, nicotine degradation bacterial strain screened from tobacco materials has relatively high biological activity and is applicable to degradation of nicotine in tobacco in a solid state, thus achieving the purpose of the degradation of the nicotine in tobacco.

The invention discloses Pseudomonas sp.HZN6 and application thereof to nicotine degradation. In application, with nicotine as a substrate, an inorganic salt nutrient solution as a reaction medium and Pseudomonas sp.HZN6 as an enzyme, the reaction is carried out for 8-15h under the conditions that temperature is 25-45 DEG C and a Ph value is 5.5-9.0 to ensure that the content of nicotine in the reaction solution is less than 0.1 percent by mass, thus the purpose of degrading nicotine is reached. The Pseudomonas sp.HZN6 can be directly added to a water body and soil to degrade nicotine in the water body and soil, and can be used for safely, efficiently and rapidly degrading residual nicotine on objects such as water and soil. A bactericide containing the Pseudomonas sp.HZN6 is prepared by a simple process with low cost, is convenient to use, and has a good application prospect.

The invention provides an agrobacterium tumefaciens SCUEC1 strain. The collection number of the strain is CCTCC NO:M2014133, and the nucleotide sequence of the strains is as shown in SEQ ID NO. 1. The strains not only can be applied to nicotine degradation, but also can accelerate the maturity of tobacco leaves. Meanwhile, the invention further provides a method for screening and separating the strains. The method comprises the following steps: performing enrichment culture, then performing separation purification, inoculating separated and purified strains to a nicotine culture medium according to a single colony, culturing in a shaking table for 1-3 days at room temperature, measuring the nicotine content of a fermentation broth of the nicotine culture medium, and secondarily screening the strains according to the magnitude of the nicotine degradation rate to obtain the agrobacterium tumefaciens SCUEC1 strains.

The invention relates to a pseudomonas fluorescens preparation and application thereof, and belongs to the field of biotechnology. A preparation method of the pseudomonas fluorescens preparation comprises the following steps of 1, selecting pseudomonas fluorescens 1206 as a production bacterial strain, wherein the pseudomonas fluorescens 1206 has a preservation number of CGMCC NO.3149, 2, carrying out strain culture through inoculating a culture solution with the pseudomonas fluorescens 1206 subjected to inclined plane culture, wherein the culture solution comprises 3% of beef extract, 0.5% of peptone, 0.5% of sodium chloride and the balance distilled water, has a pH value of 7.0 to 7.2 and is put in a shaking bottle, and carrying out culture at a shaking bottle rotate speed of 150rpm at a temperature of 28 to 30 DEG C for 18 hours to obtain a liquid fermented strain, and 3, carrying out fermentation tank fermentation through inoculating a liquid fermentation system in a fermentation tank with the liquid fermented strain, wherein the liquid fermentation system comprises 35g / L of maltose, 4.03g / L of extract yeast, 4.72g / L of ammonium sulfate, 0.4g / L of K2HPO4, 0.075g / L of MgSO4.7H2O, 0.0005g / L of FeSO4.7H2O, 0.015g / L of CaCl2 and 0.1g / L of NaCl, and carrying out fermentation under the conditions of a fermentation temperature of 27 to 29 DEG C, an inoculation amount of 5 to 10%,a fermentation tank stirring speed of 120 to 150rpm, an aeration rate of 5 to 10% and fermentation time of 24 to 48 hours. The pseudomonas fluorescens preparation has a short fermentation period and a high nicotine degradation rate, can improve tobacco quality and can be utilized for degradation of nicotine of tobacco or tobacco waste.

The present invention provides an Ochrobactrum intermedium SCUEC4 strain, wherein the preservation number is CCTCC NO:M2014403, the nucleotide sequence of the strain is represented by SEQ ID NO.1, and the strain can be applied in nicotine degradation and can further be provided for accelerating tobacco leaf maturation. The present invention further provides a method for screening and separating the strain, wherein the method comprises: carrying out enrichment culture, carrying out separation purification, inoculating the separated and purified single colony into a nicotine culture medium, carrying out shaking culture for 1-3 days at a room temperature, determining the nicotine content in the fermentation broth of the nicotine culture medium, and re-screening the strain according to the nicotine degradation rate so as to obtain the Ochrobactrum intermedium SCUEC4 strain.

The invention discloses an Acinetobacter and application thereof. Production bacterial strain liquid is fermented and cultured to form corresponding preparation. The Production bacterial strain is the Acinetobacter (Acinetobacter sp.ND12); and the bacterial strain was preserved in China General Microbiological Culture Collection Center on November 5, 2009, and a preservation number is CGMCC No.3410. The bacterial strain has stronger nicotine degradation capacity and nicotine toxicity resistance capacity, and can be grown at the nicotine concentration of 3.5g / L; and by large-scale culturing of the bacterial strain, the pure nicotine can be degraded by using a whole cell of the bacterial strain as a biocatalyst, and meanwhile the content of the nicotine in tobacco is also reduced. The Acinetobacter and the application thereof have the advantages of capacities of adjusting the content of the nicotine in the tobacco raw material, improving availability of the tobacco, degrading the nicotine in smoke dust, reducing pollution of the nicotine to environment and the like.

The invention provides a Pseudomonas monteilii SCUEC2 strain, which has a preservation number of CCTCC NO:M2014134 and a nucleotide sequence shown as SEQ ID NO.1. The strain not only can be applied to nicotine degradation, but also can accelerate tobacco leaf ripening. The invention also provides a method for screening and separation of the strain. The method comprises the steps of: firstly conducting enrichment culture, then carrying out separation and purification, and finally inoculating the separated and purified strain into a nicotine medium by single colony, performing shake cultivation at room temperature for 1-3d, then measuring the content of nicotine in the fermentation broth of the nicotine medium, and re-screening the strain according to the nicotine degradation rate, thus obtaining the Pseudomonas monteilii SCUEC2 strain.

The invention provides a new strain of nicotine degrading bacteria—Pseudomonas ZUTSKD and its application. Pseudomonas sp. ZUTSKD (Pseudomonas sp. ZUTSKD), preserved in China Center for Type Culture Collection, date of preservation is June 18, 2007, preservation number CCTCCNO: M 207083. The bacteria can degrade nicotine to produce intermediate products such as 2,3'-Bipyridine, cotinine, 3-(3,4-dihydro-2H-pyrrol-5-yl)-Pyridine and 3-Pyridinecarboxylic acid, and can tolerate the highest nicotine With a concentration of 5.5g / L, it can degrade nicotine in waste tobacco powder, and has a certain application prospect in industry.

The invention relates to a technology of biological degradation of nicotine in tobacco, in particular to a pseudomonad ZCJ bacterial strain applied to nicotine degradation of tobacco and a screening method and an application thereof. The pseudomonad ZCJ bacterial strain (Pseudomonas sp. ZCJ) was preserved in the China Center for Type Culture Collection on March, 30th, 2009 with a preservation number of CCTCC NO. M209064. The invention also discloses screening and application of the bacterial strain. In the invention, nicotine degradation bacterial strain screened from tobacco materials has relatively high biological activity and is applicable to degradation of nicotine in tobacco in a solid state, thus achieving the purpose of the degradation of the nicotine in tobacco.

The invention provides a method for detecting secondary alkaloids produced through nicotine degradation by simulating an actual environment. The method comprises steps as follows: simple sample preparation, determination of an actual environment condition simulation scheme, sampling at multiple points in time, quantitative analysis with gas chromatography-mass spectrometry, data analysis of a measured result and the like. A degradation scheme comprises two major variables, simulation of the real environmental conditions and chemical stability property of nicotine are taken into consideration comprehensively, the operation is simple, and feasibility and scientificity of the scheme are high. The detection method has the advantages of short detection time, high sensitivity and the like. With the application of the technical scheme, the nature of transformation of multi-source nicotine into secondary alkaloids can be taken into consideration comprehensively, a reliable nicotine and secondary alkaloid transformation map is obtained, and scientific support is provided for a tobacco product identification method with the secondary alkaloids as indexes in the tobacco industry.

A nicotine-containing chewing gum piece packed in a wrapping of laminate, where the laminate comprises at least an inner layer facing the chewing gum and a nicotine degradation agent barrier layer of a metal foil. The nicotine-containing chewing gum piece is shaped as an elongate plate having two ends and a thickness and a length, where the length is in the range of 8 to 20 times the thickness. The wrapping has two elongate edges sealed to one another in a fin area extending along the length of the chewing gum piece and two sealed end areas extending beyond the ends of the chewing gum piece.

The invention relates to a nicotine-degrading bacterium and an application thereof. The nicotine-degrading bacterium is characterized in that the bacterium is named as Arthrobacter histidinolovorans EA-17 according to taxonomy and was collected in China Center for Type Culture Collection on September 15, 2013, and the collection number is CCTCC No. M2013405. A strain is fermented by a 2M3 fermentation tank for production, then mixed with a self-made decomposition agent according to the mass ratio of 1:10, further inoculated into a tobacco straw compost according to the inoculation amount of 1% and fermented for 30-45d, and then straw can be decomposed; compared with traditional compost fermentation, the nicotine degradation rate is improved by 60%-78%, the total nitrogen content is increased by 7%-9%, the total phosphorus content is increased by 0.3%-0.6%, and the total potassium content is increased by 6%-8%.

The invention relates to a pseudomonas fluorescens preparation and application thereof, and belongs to the field of biotechnology. A preparation method of the pseudomonas fluorescens preparation comprises the following steps of 1, selecting pseudomonas fluorescens 1206 as a production bacterial strain, wherein the pseudomonas fluorescens 1206 has a preservation number of CGMCC NO.3149, 2, carrying out strain culture through inoculating a culture solution with the pseudomonas fluorescens 1206 subjected to inclined plane culture, wherein the culture solution comprises 3% of beef extract, 0.5% of peptone, 0.5% of sodium chloride and the balance distilled water, has a pH value of 7.0 to 7.2 and is put in a shaking bottle, and carrying out culture at a shaking bottle rotate speed of 150rpm at a temperature of 28 to 30 DEG C for 18 hours to obtain a liquid fermented strain, and 3, carrying out fermentation tank fermentation through inoculating a liquid fermentation system in a fermentation tank with the liquid fermented strain, wherein the liquid fermentation system comprises 35g / L of maltose, 4.03g / L of extract yeast, 4.72g / L of ammonium sulfate, 0.4g / L of K2HPO4, 0.075g / L of MgSO4.7H2O, 0.0005g / L of FeSO4.7H2O, 0.015g / L of CaCl2 and 0.1g / L of NaCl, and carrying out fermentation under the conditions of a fermentation temperature of 27 to 29 DEG C, an inoculation amount of 5 to 10%, a fermentation tank stirring speed of 120 to 150rpm, an aeration rate of 5 to 10% and fermentation time of 24 to 48 hours. The pseudomonas fluorescens preparation has a short fermentation period and a high nicotine degradation rate, can improve tobacco quality and can be utilized for degradation of nicotine of tobacco or tobacco waste.

The invention relates to a method of biology degradation nicotine which uses (Ochrobactrum intermedium) DN2 as bacteria seed and uses the nicotine zymosis enzyme of the Ochrobactrum intermedium DN2 or Ochrobactrum intermedium DN2 to effect on the flue-cured tobacco leaf, pipe tobacco, industrial or agricultural tobacco leftover bits and pieces and tobacco residues and the extracting liquid of the above materials to do degradation to part of or all of the nicotine.

The invention discloses a construction method and application of pseudomonas JY-Q fructose metabolism gene deleted strain. JY-Q / 5Delta is used as an original strain, the following two isozyme target genes expressing fructose phosphotransferase are determined through genome information analysis of the strain; a knockout vector of a target gene is constructed; the knockout vector is transformed intoan escherichia coli deleted strain WM3064 by heat transfer, which is a donor strain, and pseudomonas JY-Q is used as a receptor bacterium for carrying out parent conjugation; the recombinants exchanged for the first time are screened by utilizing the Kanamycin resistance gene on the vector; and after the obtained strain is subjected to overnight culture expansion, a reverse screening marker SacBgene carried on a carrier is utilized to carry out second exchange of recombinants to obtain the selectively degraded pseudomonas JY-Q fructose metabolic deleted strain. The pseudomonas JY-Q fructosemetabolism deleted strain is applied in nicotine degradation of waste tobacco leaf water extract (TWE).