3 results about "Nicotine degradation" patented technology

The present application relates to a kind of high-purity nicotine preparation method derived from tobacco stem, belong to plant extraction technical field.This method is for the large amount of tobacco stem waste produced in the process of tobacco planting and baking, proposes an efficient nicotine extraction method.By crushing tobacco stem, using acid aqueous solution for acidification treatment, then using alkaline solution to adjust pH value and carry out steam distillation, collect distillate.Subsequently, using reverse osmosis membrane concentration and molecular distillation technology for purification, obtain high-purity nicotine solution.This method does not use organic solvent, reduces the security risk, at the same time improves the extraction rate and purity of nicotine, purity can reach 95%~99%, extraction efficiency is more than 85%.In addition, the method uses membrane distillation process to reduce energy consumption, reduce the loss of active ingredients, and further remove impurities through molecular distillation technology, prevent nicotine degradation and oxidation deterioration.The implementation of the present application not only realizes the high value-added utilization of tobacco stem, but also improves the economic benefit, reduces the pollution to the environment.

This invention pertains to genetic engineering and relates to an orally administered engineered microbial system and its applications. Through genetic manipulation techniques such as genome integration, a dominant mutant of the nicotine-degrading enzyme Nox is displayed on the surface of Bacillus subtilis spores. This mutant is then coupled with catalase or peroxidase, and genes related to spore germination and the system's own recombinant enzymes are knocked out to construct a strain with self-eliminating resistance genes. This invention utilizes orally administered engineered bacteria to colonize the intestines. The "molecular degradation machine and metabolic siphon" effect produced by the engineered bacteria continuously degrades nicotine diffused from the bloodstream, thereby significantly reducing the concentration of nicotine binding to central nicotine receptors. This fundamentally weakens the dopamine reward effect induced by smoking, effectively breaking the positive feedback loop of "smoking-pleasure," and providing a new method for treating nicotine dependence. As a "plug-and-play" platform technology, displaying different degradation enzymes on the surface of Bacillus subtilis spores can also degrade other harmful substances.

The application discloses an oral composition, a mouth product, a drink and related applications of the oral composition. The oral composition provided by the application comprises a nicotine dehydrogenase, probiotics and food-grade synergistic fiber, the nicotine dehydrogenase is derived from Pseudomonas putida, and the probiotics are Lactobacillus plantarum. The oral composition provided by the application has a nicotine degradation rate of 90.5% in a simulation system. Meanwhile, the composition has excellent processability, gastrointestinal stability, high food compatibility and bioavailability.