The invention provides a method for culturing a Luzhou-flavor liquor culture solution with pit mud. The culture solution with pit mud, which is prepared by utilizing high-quality aged pit mud, an extract mixture, Daqu, yellow clay, a growth factor and liquor making by-products (distiller's grains, yellow water, and the like) is rich in beneficial liquor making microbes and nutrients, which are suitable for making liquor. The method has the beneficial effects that by adding the culture solution with pit mud during pit mud production, pit maintenance and pit rejuvenation, not only are seeds of the beneficial liquor making microbes added equivalently, so that the beneficial liquor making microbes grow and propagate rapidly in the pit mud and pits, thus increasing the contents and biological activities of the beneficial liquor making microbes in the pit mud and pits, but also the proportion and the growing environments of the nutrients, such as pH, can be regulated, the beneficial liquor making functional microbial systems in the pit mud and fermented grains can be enriched and coordinated, degradation of the pit mud can be delayed and the contents and proportion of flavor components in Luzhou-flavor liquor can be coordinated, thus finally achieving the aim of improving the quality of the liquor.