181 results about "Microbial system" patented technology

Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same.

The invention discloses a method for quickly starting a completely autotrophic nitrogen removal over nitrite process on sewage on the conditions of constant temperature and low ammonia nitrogen and belongs to the field of city sewage treatment and reutilization. The completely autotrophic nitrogen removal over nitrite (CANON) process is started in a biological filter tank reactor and comprises the following steps: firstly, nitrifying sludge by using an inoculating part, and performing aerobic nitrification start with sufficient oxygen supply to build a nitrosobacteria-dominated and nitrobacterium-dominated microorganism system; inhibiting the growth of nitrobacterium through intermittent aeration / anaerobism and enriching anaerobic ammonium oxidation bacteria; and finally, performing continuous aeration, controlling ammonia nitrogen to be oxidized to a stage of nitrous acid through restrictive oxygen supply, and optimizing microenvironment where nitrosobacteria and anaerobic ammonium oxidation bacteria coexist so as to successfully start the CANON process. By using the method, the problem that the growth enrichment of the anaerobic ammonium oxidation bacteria is slower for a long term can be solved; moreover, the starting way is simple and feasible, and the difficulty of the start of a single-stage autotrophic nitrogen removal system is reduced; and therefore, a method is provided to start the CANON process on stimulated waste water on the conditions of constant temperature and low ammonia nitrogen.

Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.

The invention discloses a composite live bacterial organism purifying agent and a method for the production thereof, the biological purifying agent contains Bacillus subtilis KX-1 CCTCC No. M 208057, Bacillus subtilis KX-2 CCTCC No. M 208058, and Bacillus subtilis KX-4 CCTCC No. M 208060, and the total bacteria count is larger than 1x10<8>CFU / g. The biological purifying agent can effectively remove ammonia nitrogen and nitrite in water and improve the water pollution; and degrade organic macromolecules and reduce the rich nutrition level in water; and the three bacilli can decompose organic macromolecule matters in water into saccharide, amino acid, vitamin, bioactive substances (hormone), etc. that can be absorbed by animals and plants, thereby realizing the complementary nutrition and harmonious symbiosis, and forming a complex and stable microbial system in water by proliferation.

The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme.

The invention relates to a method for starting a single-stage self-supported denitrification reactor, comprising the following steps: establishing a microflora with nitrosobacteria and nitrobacterium as the mainstay under the conditions of restricted supply of oxygen and small amount of available organic carbon source; then controlling ammonia nitrogen oxidization to nitrous acid phase through restricted oxygen supply so as to gather nitrosobacteria, inhibit growth of nitrobacterium and establish the microflora with the nitrosobacteria as the mainstay; optimizing the microenvironment where nitrosobacteria and anammox coexist by adjusting aeration modes and controlling dissolved oxygen level on the basis of the stable nitrous acid system, so as to promote growth and enrichment of the anammox and successfully start the single-stage self-supported denitrification reactor. The method of the invention solves the problem of inoculum source of the single-stage self-supported denitrification reactor, features simple and practical starting mode, reduced difficulty of starting the single-stage self-supported denitrification reactor and shortens times for starting the single-stage self-supported denitrification reactor.

The invention discloses a method for treating wastewater by utilizing a composite microbial preparation, which comprises the following steps of: putting the composite microbial preparation in an amount of 0.15 to 0.3 percent based on the treatment volume of a biochemical basin into the biochemical basin, and putting diatomite in powder or powdery activated carbon in an amount of 1 to 2 percent based on the volume of the basin into the basin. Through the method, the composite microbial preparation and an activated carbon filler can be directly put and added into the biochemical reactor once, thereby effectively promoting the establishment of an entire food chain of a microbial system and the complete degradation of various pollutants, promoting microbes to utilize nutrient substances in water and realizing the high-efficiency removal of the pollutants, such as organic matters (CODcr), ammonia nitrogen and the like.

The invention discloses a complex microbial agent for composting fermentation and application thereof. The complex microbial agent is a solid inoculant which comprises phanerochaete chrysosporium, streptomyces griseorubens, bacillus subtilis, bacillus methylotrophicus and bacillus amyloliquefaciens. The complex microbial agent is a complex microbial system comprising various microorganisms which have complementary and synergistic functions; the microbial system integrates quick fermentation heat production, biological deodorization and compost material decomposition and degradation and has the effects of a biological fertilizer; the livestock manure compost can be quickly heated to kill off harmful organisms at high temperature; the unpleasant smell is obviously reduced to solve the problem of environment pollution caused by poultry excrement; the microorganisms can be quickly propagated in the poultry excrement fermentation process; the organic matters such as lignocellulose in the excrement can be quickly and effectively decomposed so as to increase the decomposition speed of the compost material and improve the quality of the compost product; the complex microbial agent can be used for producing the biological organic fertilizer so as to reduce the agricultural production cost.

The invention belongs to the field of treatment and regeneration of urban sewage, and provides a quick starting method for a technology for simultaneous denitrification and COD removal under the condition of normal-temperature and low-C / N sewage. The method comprises the steps as follows: firstly inoculating some mature CANON sludge, and enabling the CANON sludge to perform biofilm culturing on volcanic rock filtering material, and quickly constructing an aerobiotic AOB and anammox oriented microbial system; then reducing the inflowing water substrate concentration and adjusting the aeration and hydraulic retention time, so as to improve the nitrogen removal load, and realize the high-load stable operation of the CANON technology under the condition of a medium ammonia nitrogen substrate; finally adding an organic carbon source into the inflowing water, performing heterotrophy for the growth of denitrifying bacteria, and controlling the temperature, free ammonia, aeration and other conditions, so as to optimize the aerobiotic AOB, anammox and denitrifying bacteria coexisted micro-environment, and successfully start an SNAD technology. According to the invention, the difficult problems that a large amount of organic carbon sources are required to be added for biological denitrification in the long term and the cost is prodigious are solved, and a method is provided for starting of the SNAD technology under the condition of normal-temperature and low-C / N simulated wastewater.

The invention discloses a compound microbiological preparation and application thereof in the waste water treatment. Strains adopted by the invention can be conveniently obtained from a natural world by technicians in the field according to the conventional strain identifying and separating method, or can be publicly purchased by a commercial channel. All culture solutions and microbial carriers are mixed according to a certain proportion to obtain the compound microbiological preparation. The compound microbiological preparation disclosed by the invention contains 38 strains; the compound microbiological preparation can be directly fed in a biochemical reactor at one time, so that the establishment of a complete food chain in a microbial system can be effectively promoted, the utilization of microorganisms for nutrient substances in water is promoted, and high-efficiency removal of polluted substances such as organic pollutants (such as benzene ring halogenated compounds), ammonia, nitrogen and the like is realized. The compound microbiological preparation is suitable for biochemical treatment of waste water which is high in salt content, contains heterocyclic aromatic hydrocarbons and is difficult to degrade.

Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and / or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.

The invention discloses a screening and construction method for a composite microbial system for rapidly degrading rice straw and a composite microbial system, which belong to the technical field of microbial screening methods. The method comprises the following steps of: (1) constructing a strain pond; (2) preparing a solid culture medium; and (3) screening and constructing the composite microbial system. The composite microbial system screened by the method comprises bacteria and fungi. The method and the composite microbial system have the advantages that: the composite microbial system is obtained by screening under the condition of the solid culture medium, comprises the bacteria and the fungi and has better decomposition effect; the composite microbial system RSS-4 is screened under the condition of unsterilized solid culture medium; the screened composite microbial system has better decomposition effect at the intermediate temperature, namely the temperature of 22 DEG C; and the defect that the composite strain can only decompose at the temperature of 28 DEG C existing in the prior art is effectively overcome.

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbialbacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

The invention discloses a screening and domesticating method of a composite microbial system for low-temperature degradation of straws. The screening and domesticating method comprises the steps of acquiring a natural material sample rich in cellulose in an alpine area in which the annual average temperature is -0.6-8 DEG C, carrying out enrichment culture on a cellulose degradation microbial system, then carrying out restrictive subculture preliminary screening, low-temperature gradient induced domestication and cellulase enzyme activity re-screening, and then carrying out low-temperature multi-generation restrictive carbon source subculture domestication to obtain the composite microbial system for low-temperature degradation of straws. The screening and domesticating method can be used for screening and domesticating the composite microbial system for high-efficiency degradation of straws at low temperature and has an important meaning in field returning and utilization of straw resources in the northern area.

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

The invention discloses a method for starting an anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater. Anaerobic sludge and aerobic sludge are inoculated to a reactor, starting strategies are as follows: at first, anaerobic ammonium oxidation bacteria are enriched in the reactor through maintaining the conditions of anaerobism, organic carbon source-free feed water and no saltness, and a microflora system which takes the anaerobic ammonium oxidation bacteria as dominant bacteria is built; then, salt-tolerant anaerobic ammonium oxidation bacteria are preferentially selected under the conditions of low nitrogen concentration and low saltness, non-dominant bacteria are elutriated, and a microflora system which takes the salt-tolerant anaerobic ammonium oxidation bacteria as dominant bacteria is built; and finally, the nitrogen concentration and saltness are gradually increased, so that the ammonium oxidation bacteria can be suitable for high nitrogen concentration and high saltness environments. Through a mode of gradually changing a feed water base material, the problem of slow anaerobic ammonium oxidation bacteria enrichment under saltness inhibition is solved, the starting mode is simple and practicable, and the difficulty in starting a high-salt wastewater anaerobic ammonium oxidation treatment system is lowered.

The present invention relates to environment protecting technology, and is especially the starting method of biological denitrogenation reactor. The present invention provides one kind of fast and efficient method of starting anaerobic ammoxidation reactor and culturing microbe, i. e., aerobic starting. In the continuous culture mode, fixed bed biological membrane reactor as culture device, aerobic active sludge as bacteria seed, ammonia nitrogen waste water as culture liquid are adopted to culture biomembrane in aerobic condition. The culture process is controlled in the nitrifying stage, and after the nitrifying activity of the microbial system is strengthened gradually, nitrifying bacteria in the biomembrane are enriched and the nitrifying biomembrane is aerobically cultured, aeration is stopped before starting anaerobic ammoxidation.

The invention relates to a construction method of a live branch frame type plane source retaining purified ecological revetment. The method comprises the following steps of: paving rock stones on an included angle position of a riverbank slope surface and a riverbed; bundling epicormic branches of willows, clethraceae and black poplars into live branch bunches; since the live branch bunches form a live branch frame, paving the live branch frame on the riverbank slope surface along the block stones, paving live branch layers and live branch frame layers in a staggered way layer by layer on the live branch frame layers; and paving filling in the top most layer of frame. The invention aims to solve the problems that a natural slope protection way has poor stability, hard slope protection causes adverse effects on the ecology, the environment and the landscape of a revetment, and the ecological slope protection landscape is excessively single and too high in cost. The revetment is completely constructed by natural materials, builds a fine riverbank biological habitat environment and ensures the stability of the revetment; in addition, beautiful natural landscapes are formed on a riverbank revetment, and a plant-gravel-microorganism system formed on the revetment has better retaining and purifying effects on pollutants entering into a river channel in a plane source way.

The invention discloses a micro-aeration enhanced ecological floating bed sewage treatment device which comprises water plants, a floating carrier, a combined packing layer, a fine bubble aeration pipeline, a support and an aeration machine, wherein plant holes are formed in the floating carrier, the water plants are planted onto the floating carrier through the planting holes and float on the water surface inside the micro-aeration enhanced ecological floating bed sewage treatment device, the combined packing layer is arranged below the floating carrier, the fine bubble aeration pipeline is circularly laid at the bottom of the micro-aeration enhanced ecological floating bed sewage treatment device through the support, and the aeration machine is connected with the fine bubble aeration pipeline at the bottom of the device. Aeration for the device is performed through a micro-aeration system, so that the growth of microbes on plant roots and the combined packing layer is promoted, the synergistic effect of a microbial system and a water plant system is promoted, the device can be used repeatedly, pollution to water cannot be caused, the cost is low, and the device is very suitable for being applied in a large area during natural water body pollution treatment.

The invention provides a method for culturing a Luzhou-flavor liquor culture solution with pit mud. The culture solution with pit mud, which is prepared by utilizing high-quality aged pit mud, an extract mixture, Daqu, yellow clay, a growth factor and liquor making by-products (distiller's grains, yellow water, and the like) is rich in beneficial liquor making microbes and nutrients, which are suitable for making liquor. The method has the beneficial effects that by adding the culture solution with pit mud during pit mud production, pit maintenance and pit rejuvenation, not only are seeds of the beneficial liquor making microbes added equivalently, so that the beneficial liquor making microbes grow and propagate rapidly in the pit mud and pits, thus increasing the contents and biological activities of the beneficial liquor making microbes in the pit mud and pits, but also the proportion and the growing environments of the nutrients, such as pH, can be regulated, the beneficial liquor making functional microbial systems in the pit mud and fermented grains can be enriched and coordinated, degradation of the pit mud can be delayed and the contents and proportion of flavor components in Luzhou-flavor liquor can be coordinated, thus finally achieving the aim of improving the quality of the liquor.

Provided are improved recombinant microorganisms, and methods of use thereof, for metabolizing biomolecules and producing commodity chemicals such as ethanol therefrom, and genetic constructs to achieve that end.

The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21.

Methods and systems to achieve clean fuel processing systems in which carbon dioxide emissions (1) from sources (2) may be processed in at least one processing reactor (4) containing a plurality of chemoautotrophic bacteria (5) which can convert the carbon dioxide emissions into biomass (6) which may then be used for various products (21) such as biofuels, fertilizer, feedstock, or the like. Bacteria that reduce oxidized nitrogenous species (13) may be used to supply reduced nitrogenous compounds to the chemoautotrophic bacteria (5).

The invention discloses a lake eutrophication remediation method, which comprises the following steps of: (1) sequentially arranging a microbial remediation water tank, an absorption water tank and a cooling water tank on lakeshore in a half-burying way; (2) performing microbial remediation; (3) performing absorption; (4) performing cooling; and (5) performing ecological remediation in water. In the microbial remediation stage of the method, microorganisms grow stably in a certain controllable environment so as to achieve high microorganism survival rate; the microorganisms form a biomembrane system in a filler to achieve strain diversity; the microorganism system rapidly reduces the chemical oxygen demand (COD), biochemical oxygen demand (BOD) and ammonia nitrogen of organic pollutants without producing organic sludge and causing secondary pollutions; the method ensures high water purification dephosphorization efficiency; and exception handling for realizing a temperature reduction function is performed in the high temperature environment of a water bloom peak period.

The invention provides a method for preparing a soil restoration modifier by the utilization of spore fermentation wastewater, comprising the following steps: inoculating a composite strain to a medium and carrying out anaerobic cultivation to obtain a composite microbial system; and inoculating the composite microbial system to a fermentation medium and carrying out anaerobic fermentation to obtain the soil restoration modifier. The soil restoration modifier contains a plurality of active microorganisms, enzyme, organic acid and antibiotics, can play a role in promoting a benign micro-ecological environment in crop soil, can be used to solubilize organic and inorganic nutrients, promote nutrient absorption and conversion of crops, improve granular structure of soil and simultaneously inhibit propagation of harmful pathogen. Pesticide residues in soil are decomposed to some extent. By the method, the purpose of emission reduction can be achieved, and the environment is protected. In addition, nutritional ingredients in wastewater can be fully used in the production of the soil restoration modifier. Cost and water resources are saved. The method is simple and easy to operate, has low operation cost and is easy for industrial application.