18 results about "Microbial system" patented technology

The application discloses a method for improving the recovery ratio of low-permeability oil reservoirs by using microbial composite pressure flooding, and comprises the following steps: S1, test block screening; S2, microbial system screening and performance evaluation; S3, pressure flooding injection process optimization; and S4, field application. The application is suitable for the technical field of oil reservoir development, and the recovery ratio of low-permeability oil reservoirs can be effectively improved by using the microbial composite pressure flooding mode. The injected microbial system can reduce the viscosity of crude oil, reduce the injection pressure, increase the injection amount, and reduce the oil-water interfacial tension. The pressure flooding injection mode can not only rapidly supplement the formation energy, but also form micro cracks and expand the sweep range of the microorganisms. The injected microbial system is non-toxic and harmless, has no influence on the subsequent sewage treatment, and can avoid the problem of environmental pollution caused by the injection of chemical reagents. The application has the advantages of simple construction process, simple operation, high input-output ratio, and good field test effect.

The invention discloses a fungal preparation for improving the fermentation performance of monascus purpureus and application of the fungal preparation. The fungus preparation is prepared by compounding Aspergillus niger MQM20136, Monascus purpureus 40269 and Saccharomyces cerevisiae JH301 direct vat set fungicide, the Aspergillus niger MQM20136 is preserved in the China Center for Type Culture Collection in Wuhan, the preservation time is September 25, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20252120. According to the invention, a fungus preparation taking the aspergillus niger MQM20136 as a main body is constructed, a mutualism and synergistic interaction microbial system is formed in the fungus preparation, and when the fungus preparation is used for pure compound preparation of the monascus purpureus, the quality stability, the esterification capacity, the wine yield and other fermentation properties of the monascus purpureus can be improved.

The application discloses a gas hydrate reservoir microbial reinforcement mining model test device and a test method. The gas hydrate reservoir microbial reinforcement mining model test device comprises a reaction kettle, an injection control system, a seawater supply system, a gas supply system, a microorganism supply system, a cementing fluid supply system, a sensor assembly and a hydrate mining system; the sensor assembly is used for acquiring the temperature, pressure, water content, hydrate saturation, deformation, strength and mineral component distribution of the hydrate reservoir in the reinforcement-decomposition process. The technical scheme of the application provides a test device which can observe the hydrate reservoir seepage field, temperature field, deformation field, hydrate and mineral content distribution evolution law in the process of the microbial reinforcement natural gas hydrate reservoir and mining in real time.

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The application is a pig farm wastewater treatment facility and system integrating efficient deodorization and ecological landscape, the technology constructs clear zoning of underwater anaerobic and water surface aerobic, and innovatively designs a surface water body convergence network to optimize the surface water flow path, strengthen the water body exchange rate, and accelerate the construction of the surface aerobic area microbial system. At the same time, the plant system is deeply integrated into the whole process of wastewater treatment process, which forms a good on-site landscape, effectively intercepts, absorbs and converts odor gas, and improves the environment of the site. The treatment device of the application is very simple, has no operation cost, fundamentally solves the odor problem in the process of pig farm wastewater treatment, and realizes the systematic improvement of environmental friendliness and sensory effect on the basis of ensuring efficient treatment effect.

The present application belongs to the technical field of microbial solid-state fermentation, and relates to a determination method for the mutual action relationship of a microbial flora in a compound microbial system and application thereof. The determination method comprises the following steps: performing solid-state fermentation on each single bacterium in the compound microbial system, and extracting the maximum thermal power and total heat production of each single bacterium; performing solid-state fermentation on the compound microbial system, and extracting the measured maximum thermal power and measured total heat production of the compound microbial system; based on the inoculation proportion of each single bacterium in the compound microbial system and the thermal spectrum data of the single bacterium, the theoretical maximum thermal power and theoretical total heat production are calculated; the mutual action relationship of the microbial flora is determined according to the difference between the maximum thermal power and total heat production of the compound microbial system and the theoretical maximum thermal power and theoretical total heat production. The determination system capable of distinguishing different cooperation modes is established by combining the total heat production and maximum thermal power parameters, the thermal spectrum data is monitored in real time throughout the whole process, the dynamic tracking of the mutual action of the microbial flora is realized, and engineering guidance is provided for precisely regulating the fermentation process and directionally optimizing the function of the microbial flora.

The application is a microbial self-repairing concrete mix proportion design method and design system, the core of which is to establish a quantitative design model with the goal of maximizing microbial repair potential, and to realize the collaborative optimization with conventional concrete mix proportion parameters. First, the required calcium carbonate generation amount is determined based on the target crack size; then, the optimal microbial and nutrient content is calculated through the established microbial metabolism kinetics model; the key is to establish the correlation model of the microbial system as a key factor affecting the water-cement ratio and fluidity and the dosage of the additive, and to perform integrated calculation of the water-cement ratio-aggregate system-additive. The application realizes the leap of self-repairing concrete mix proportion from empirical exploration to quantitative, precise and integrated design.

The invention discloses a water body treatment device and method based on a water body microbial system. Comprising a bed frame, a floating body detachably installed in the center of the top end of the bed frame, a plurality of connecting rod type floating balls annularly installed on the outer wall of the floating body at equal intervals, a layer sleeve type coiling frame arranged in the center of the bottom end of the bed frame and a plurality of suspended loading boxes connected to the left side and the right side of the top of the bed frame in an interference sliding mode, and biological wax is contained in the suspended loading boxes. And a plurality of turns of braid type biological ropes are wound on the peripheral surface of the layer sleeve type coiling frame. According to the invention, a water body microorganism treatment system mainly comprising the aquatic vegetation layer, the biological wax and the braid type biological rope can be quickly formed, the bed frame is subsequently lifted into a water body to be treated and suspended through the floating body, and the aquatic vegetation layer, the biological wax and the braid type biological rope are all submerged into the water body.

The invention relates to the technical field of organic wastewater treatment, and discloses an electrically-assisted microbial system and an application method thereof in monofluoronitrobenzene pollution control, and the method comprises the following steps: S1, treating wastewater by adopting the electrically-assisted microbial system through sequencing batch operation; the electrically-assisted microbial system comprises a reactor with the effective volume not smaller than 3 L, an aeration device and a power supply, electrodes composed of anodes and cathodes are arranged in the reactor, and the distance between the electrodes is 1 + / -0.2 cm; the power supply is electrically connected with the electrodes through titanium wires; the anode is a titanium mesh, and the cathode is formed by attaching microorganisms to a stainless steel mesh; s2, establishing a quadratic polynomial model of the initial concentration, carbon nitrogen ratio and voltage of p-fluoronitrobenzene by using a response surface method, and optimizing to obtain operation parameters; and setting operation parameters to operate the electrically-assisted microbial system to complete the treatment of the fluoronitrobenzene wastewater. The problems of low efficiency, poor stability and the like of a traditional treatment technology in large-scale application are solved.

This invention proposes a rapid artificial microbial community identification method based on near-infrared spectroscopy and machine learning. First, tagged artificial microbial community samples are constructed, fixed using a filter membrane, and then raw near-infrared spectra are collected. Standardization processing based on white / black board reference correction and absorbance calculation is performed. Data augmentation is applied only to the training set, and then EDCN spectral preprocessing is performed on the test set and the augmented training set. The PCA projection model is fitted using the EDCN-preprocessed training set to obtain a well-fitted PCA projection model. Using the training set as input, low-dimensional PCA features are extracted using the fitted PCA projection model. These low-dimensional features are then used as input to train a liquid neural network, resulting in a trained liquid neural network. Finally, using the test set as input, the microbial community identification results are obtained based on the fitted PCA projection model and the trained liquid neural network. This method enables rapid monitoring of complex mixed microbial systems.

This invention discloses a biocontrol compound microbial strain YC and its application, relating to the field of microbial technology. The biocontrol compound microbial strain YC is composed of *Trichoderma harzianum* YP2 and *Trichoderma viride* CK1A. *Trichoderma harzianum* YP2 was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and is classified as follows: Trichoderma afroharzianum YP2, with accession number CGMCC NO. 41947; the described *Trichoderma viride* CK1A was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and classified as... Trichoderma atroviride CK1A, with accession number CGMCC NO. 41948. The biocontrol compound microbial system YC described herein is used to control maize stalk rot. The biocontrol compound microbial system YC of this invention exhibits stable efficacy and strong antibacterial ability, opening up new avenues for the biological control of maize stalk rot in my country.

The invention relates to the technical field of microbial control for cubilose peptide production, and discloses a microbial system for controlling the whole production process of cubilose peptide. A data acquisition module of the system is responsible for acquiring microorganism concentration data and process parameter data of each batch; the reference group determination module determines a reference batch set for the target batch; the sensitivity calculation module calculates the sensitivity index of the target batch at the monitoring point according to the data distribution of the reference batch set; the influence score calculation module calculates the initial influence score of the monitoring point according to the global data distribution, and obtains a real influence score through batch number and sensitivity index adjustment; the key point screening module obtains a background interference level by comparing a reference set with global process parameter distribution, and screens out key monitoring points in combination with a real influence score; a microorganism control model is established based on data of key monitoring points and is applied to production monitoring, and precise control and systematic management of the microorganism level in the cubilose peptide production process are achieved.

The invention discloses a method for identifying and analyzing microorganisms and bacteriophages participating in perchlorate reduction in perchlorate polluted wastewater. Microbial system development information capable of driving the ClO4 <-> reduction process in a culture system is identified by using a DNA-SIP technology, then the metabolic potential of ClO4 <-> reduction microorganisms is evaluated by using a box-separating assembly technology, and the species information of the bacteriophage and the auxiliary contribution of the bacteriophage to the ClO4 <-> reduction function microorganisms are mainly analyzed by combining the deep analysis of the bacteriophage. The method disclosed by the invention has great significance for cleaning the ClO4 <-> reduction function microbial group of sludge and driving the ClO4 <-> reduction process by the synergistic bacteriophage of the microbial group, and understanding the ClO4 <-> reduction function bacteria and key functional genes for driving the ClO4 <-> reduction by the synergistic bacteriophage of the ClO4 <-> reduction function bacteria.

A device for the supply or discharge of medium into or out of a culture vessel comprises a housing on which a first aperture and a plurality of second apertures are arranged. The first aperture and the second apertures have fluid-conducting connection to one another in a manner to supply a medium from the first aperture via the second apertures into the interior of the culture vessel or to discharge same in reversed direction therefrom, when the device is attached on the culture vessel. The plurality of second apertures are configured to generate a plurality of medium sub-streams arranged in parallel to one another of the medium that is to be supplied or that is to be discharged. Further disclosed are a culture vessel and a method of cultivating microbiological systems.

This invention discloses a biocontrol compound microbial system YK and its application, relating to the field of microbial technology. The biocontrol compound microbial system YK consists of *Trichoderma harzianum* YP2 and *Bacillus subtilis* K14. *Trichoderma harzianum* YP2 was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and is classified as follows: Trichoderma afroharzianum YP2, with accession number CGMCC NO. 41947; the described Bacillus subtilis K14 was deposited at the China General Microbiological Culture Collection Center on May 22, 2025, and classified as... Bacillus subtilis K14, with accession number CGMCC NO. 34540. The biocontrol compound microbial system YK described herein is used to control maize stalk rot. The biocontrol compound microbial system YK of this invention exhibits stable efficacy and strong antibacterial ability, opening up new avenues for the biological control of maize stalk rot in my country.

The invention relates to a microorganism self-repairing concrete mix proportion design method and a design system, which are characterized in that a set of quantitative design model taking microorganism repairing potential maximization as a target is established, and collaborative optimization with concrete conventional mix proportion parameters is realized. Firstly, the required calcium carbonate generation amount is determined based on the target crack size; then, calculating the optimal mixing amount of microorganisms and nutrients through the established microbial metabolism kinetic model; the method is characterized in that the microbial system is used as a key factor influencing the water cement ratio and the fluidity, a correlation model of the microbial system and the dosage of the admixture is established, and integrated calculation of the water cement ratio, the aggregate system and the admixture is carried out. According to the method, the self-repairing concrete mix proportion spanning from empirical exploration to quantitative, precise and integrated design is realized.

This invention discloses a floating island root purification device induced by hydrodynamic response and biochemical zoning, comprising a double-cylinder structure connected to the floating island for purifying the roots of floating island plants; the double-cylinder structure includes an inner cylinder, an outer cylinder, and an annular reaction chamber fitted over the roots of the floating island plants; the annular reaction chamber is equipped with a microbial system for denitrifying the roots of the floating island plants and providing a carbon source; the outer cylinder is provided with buffer spikes for mitigating water flow impact and facilitating the transport of substances by the microbial system; this invention, by organically combining structural zoning and microbial colonization, systematically solves the problems of easy loss, difficult colonization, and low efficiency of traditional floating island inoculants.

The application provides a culture method of a highland adaptive denitrification composite microbial agent and belongs to the technical field of water treatment and resource utilization. In view of the existing problem that the metabolism of sewage denitrification functional bacteria is limited under the combined stress of low temperature and low oxygen in the highlands, a specific composite microbial system with low-temperature and low-oxygen adaptability is constructed, the strain source is obtained from the in-situ environment of the highlands, the high-throughput sorting technology is used to efficiently and accurately obtain the functional single bacteria in the simulated highland environment, the best strain matching and inoculation ratio are determined through the control experiment, the synergistic denitrification of the composite strains is realized, the method can be used as a biological strengthening agent to enhance the denitrification effect of the highland sewage treatment unit, and has a wide application prospect.