46 results about "Microbial colonization" patented technology

The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.

The invention relates to a special fertilizer suitable for saline-alkali soil and a preparation method thereof. The fertilizer is granular and has a multi-layer structure which is sequentially arranged from inside to outside: a nutrition core layer, a controlled release film layer, a soil improvement fast exchange layer and a microorganism attaching layer. The nutrition core layer contains nitrogen, phosphorus, potassium and medium trace elements, and meets the basic nutrient requirements of crops; the controlled release film layer is self-assembled layer by layer by adopting alginate / chitosan, a humic acid-ferrous complex and a breakable cross-linking point are inserted into the film, and starch is sprayed on the outer layer, so that an ionic strength / alkalinity dual-response controlled release system is formed; the soil improvement quick exchange layer contains microcrystalline gypsum and ferrous sulfate; the microbial epiphytic layer fixes salt-tolerant phosphate solubilizing bacteria, potassium bacteria and exopolysaccharide-producing bacillus by using a charcoal-zeolite carrier, and the bacteria are distributed on the surfaces of the particles in a dotted manner. The fertilizer realizes organic combination of soil improvement, fertilizer supply and microbial regulation and control, can significantly reduce the soil salinity and alkalinity in a saline-alkali soil environment, and improves the fertilizer utilization rate and the crop growth performance.

The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.

Systems and methods for infection prevention and treatment in biological conduits using electroporation or pulsed field ablation (PFA). The system includes an elongate member, such as a dialysis catheter, vascular access device, or peritoneal dialysis conduit, with at least one electrode positioned along, within, or around the member. A control unit applies pulsed electric fields to inhibit microbial colonization, disrupt bacterial biofilms, and alter permeability of bacterial or endothelial cells to enhance sterility or fluid exchange. The system may use real-time impedance monitoring, microbial detection, or fluid composition analysis to adjust electroporation parameters. Electroporation may synchronize with dialysis cycles or antimicrobial lock solutions. The method includes automated, clinician-controlled, or sensor-triggered treatments to reduce infections and improve device longevity. The system provides an alternative or augment to antibiotics, reducing the risk of drug-resistant infections while improving the longevity and safety of medical implants.

This invention relates to the field of electrochemical technology, and particularly to a single-atom nickel-modified electrode material, its preparation method, and a reactor. An embodiment of this invention provides a method for preparing a single-atom nickel-modified electrode material, comprising: S1, subjecting a carbon felt to a surface hydrophilic treatment to obtain a hydrophilic carbon felt; S2, immersing the hydrophilic carbon felt in a first solution containing Ni ions, and adding a second solution containing organic matter, allowing for a complete reaction, and then growing a nickel-containing metal-organic framework material in situ on the hydrophilic carbon felt; S3, calcining the hydrophilic carbon felt containing the nickel-containing metal-organic framework material to obtain the single-atom nickel-modified electrode material. This invention provides a single-atom nickel-modified electrode material, its preparation method, and a reactor, which can provide a cathode material that combines efficient microbial colonization with interfacial stability.

The invention provides an air purification material and a preparation method thereof, a purification filter element, an air conditioning system and a vehicle, and belongs to the technical field of air purification, and the air purification material comprises a coating layer and a microorganism colonization inner core. The coating layer is loaded with a microcapsule, the microcapsule comprises a wall material and a nutritional core material coated in the wall material, the microorganism colonization inner core is coated in the coating layer, and the microorganism colonization inner core comprises a porous base material and gaseous pollutant degrading bacteria loaded on the porous base material. Wherein the nutrition core material provides nutrition for the gaseous pollutant degrading bacteria. The air purification material provided by the embodiment of the invention can maintain long-term and stable gaseous pollutant degradation performance.

The invention discloses a microbial agent for preventing and treating cotton verticillium wilt and a preparation method and application thereof, the microbial agent comprises a bacterial strain of bacillus velezensis and / or a fermentation broth thereof, and an agriculturally acceptable solid carrier for loading the bacterial strain, and the preservation number of the bacterial strain is CCTCC NO: M 2022954. According to the microbial agent, bacillus velezensis BAC81712 serves as an antagonistic bacterium and is loaded on a solid carrier, and the microbial agent has the advantages of being convenient to use, low in microorganism colonization difficulty, high in bacteriostasis rate, stable in prevention and treatment effect and the like, can be widely used for preventing and treating cotton verticillium wilt in potted plants and fields, can remarkably reduce the morbidity of the cotton verticillium wilt and has a good application prospect. The cotton plant morphology can be obviously improved, the use value is high, and the application prospect is good.

Disclosed are adhesive medical films incorporating one or more cationic steroidal antimicrobial (CSA) compounds. The adhesive medical film is in the form of a tape, dressing, or sheet, configured for contact against dermal tissue to provide securement of medical devices, wound care, wound closure, and / or scar prevention. The adhesive medical film includes (i) a polymer adhesive (e.g., pressure-sensitive adhesive) in which one or more CSA compounds are distributed, and (ii) optionally, a backing substrate upon which the polymer adhesive is disposed. Examples of polymer adhesives include polyacrylate and silicone adhesives. The adhesive medical films, when used, reduce microbial colonization and / or growth, both on the films during storage and at a dermal site, as compared to the same adhesive medical film without incorporated CSA compounds.

The present application relates to the technical field of landscaping engineering, and relates to a multi-stage ecological self-repairing type saline-alkali land greening method, which comprises the following steps: spreading composite soil microcapsules containing a salt absorption agent, slow-release nutrient elements and an organic substrate on a soil surface layer and a 5-10 cm middle layer, laying a guest soil layer on the composite microcapsule layer, covering a layer of degradable biological membrane, and fixing the biological membrane with nails or stones; selecting a plurality of salt-tolerant plants to form a tree layer, a shrub layer and a ground cover layer, configuring a microbial suspension, uniformly spraying the microbial suspension on a plant rhizosphere area by using a spraying device, subsequently irrigating to help the microbial colonization, replacing plant species with different depth root systems every 2-3 years, and alternately planting shallow-rooted plants and deep-rooted plants to break the accumulation of salt in a specific area of soil. The present application realizes the gradual improvement of saline-alkali land through a multi-stage ecological repair system, can dynamically adapt to the change of soil salinity at different stages, and effectively alleviates the salt condensation problem.

The invention provides a biochar-based microbial composition and application thereof in soda saline-alkali soil, and belongs to the technical field of soil improvement. The invention provides a biochar-based microbial composition. Microorganisms for producing exopolysaccharides and the exopolysaccharides are fixed in a graded pore structure of biochar. According to the method, biochar with a hierarchical pore structure is taken as a carrier, microorganisms with exopolysaccharide (EPS) secretion capacity are colonized in the deep positions of mesopores and macropores in the biochar in a vacuum impregnation manner, and strains are subjected to in-situ synthesis in advance in the pores of the biochar and secrete a large amount of EPS. According to the biochar-based microbial composition, through dual protection of a biochar physical barrier and an EPS chemical barrier, the survival rate of strains is remarkably increased, and the soil improvement effect is achieved.

The invention discloses a microorganism colonization membrane assembly for river regulation, and relates to the technical field of river regulation, the microorganism colonization membrane assembly comprises a mounting box with an opening at the upper end, two partition plates are fixedly mounted in the mounting box at intervals, and the two partition plates sequentially divide the mounting box into a microorganism enrichment area, a metabolism diffusion area and a fluid exchange area from front to back; the three regions are respectively filled with a microorganism colonization membrane, a fluid flow guide membrane and a honeycomb membrane, and the side wall of one side, far away from the partition plates, of the microorganism enrichment region and the two partition plates are respectively provided with water permeable holes through which water flows to pass; the surface of the microbial colonization membrane is provided with nano-scale villus-shaped secondary ripples. According to the microorganism colonization membrane assembly for river regulation, through cooperation of the biological anchor points and the chemotactic factor coating, the microorganism attachment efficiency (the survival rate is increased by 40% or above) and the impact load resistance are remarkably improved, and the problems that in traditional biological remediation, microorganism colonization is difficult, and activity is low are solved.

The present invention discloses a method and application of using polylactic acid plastic to cooperate with rhamnolipid to improve sewage treatment efficiency, and belongs to the field of water treatment and environmental protection technology. The present invention purifies and cultures the extracted microorganisms, and then conducts colonization experiments of microorganisms, density determination of suspended microorganisms, concentration determination of microorganisms on the surface of polylactic acid, and analysis of microbial community structure. According to the experimental results, the optimization effect of using polylactic acid plastic to cooperate with rhamnolipid to improve sewage treatment efficiency is evaluated. The method uses polylactic acid plastic pretreated with rhamnolipid as a filler for microorganisms. Through the synergistic effect of polylactic acid plastic and rhamnolipid, the colonization amount and activity of microorganisms on the surface of polylactic acid plastic are improved, the density of suspended microorganisms is increased, and the characteristic that polylactic acid particles are easily colonized by microorganisms after rhamnolipid is attached is brought into play, so as to adjust the proportion of microorganisms such as degrading complex organic matter and nitrifying bacteria, and optimize the application of sewage biochemical treatment process.

The invention discloses a bio-based fertilizer and application thereof. The preparation method comprises the following steps: S1, crushing the mango pomace and the bagasse, sieving the crushed mango pomace and bagasse with a 40-60-mesh sieve, adding diluted hydrochloric acid and ferrous sulfate, mixing the crushed mango pomace and bagasse, carrying out a hydrolysis reaction, and filtering a hydrolysis product to obtain solid residues; s2, seed liquid of bacillus coagulans, bacillus pseudomycoides, trichoderma viride and saccharomyces cerevisiae is inoculated into the mixture of the solid residues and the cattle and sheep manure for fermentation, and a fermentation product is obtained; s3, mixing the fermentation product, charcoal and polyglutamic acid to prepare the fertilizer. The mango pomace, solid bagasse and cattle and sheep manure mixture are cooperatively fermented through compound bacteria, the porosity of the fertilizer is increased, the water and fertilizer retention capacity is improved, the fermentation product, biochar and polyglutamic acid are mixed and granulated to prepare the fertilizer, the disintegration rate can be regulated and controlled, nutrients are prevented from being instantly released, and root growth and microorganism colonization are promoted.

The invention discloses a PHA (polyhydroxyalkanoate)-based barrier porous collaborative potted seedling culture cup suitable for natural compost treatment as well as a preparation method and application thereof, and belongs to the technical fields of biodegradable polymer materials, gardening container structure engineering and natural compost treatment. Aiming at the industrial pain point that an existing biodegradable container is difficult to consider both wet durability and compost degradation speed, an asymmetric gradient structure which is sparse outside and hydrophilic inside is innovatively constructed in the scheme. A high-efficiency bio-based barrier technology is adopted on the outer side, compact protection can be formed only by an extremely low additive amount, and excellent water control performance and structural strength comparable to those of petrochemical plastics are achieved; a hydrophilic porous system is specially arranged on the inner side, and actively absorbs water in a composting environment to initiate micropore disintegration, so that the microbial colonization efficiency is greatly improved, root penetration is guided, and the technical barrier that a high-barrier material is difficult to degrade is overcome. The product is fully biodegradable, fluoride-free and safe, perfectly realizes the unification of firmness and reliability in the use period and rapid and natural returning after being discarded, and has a wide application prospect.

The application belongs to the technical field of agricultural microbiology and plant protection, and discloses application of an immunosuppressive active substance, and aims to solve the problem that there is no immunosuppressive substance for effectively suppressing plant immunity, improving the colonization efficiency of microorganisms on crops, and improving the biological prevention and control effect in the prior art. The immunosuppressive substance comprises the following applications: application in inhibiting plant immunity, application in promoting plant microbial colonization, application in plant disease biological prevention and control, and application in preparation of agricultural microbial agents, biological prevention and control agents and / or growth promoting agents. The application is suitable for crop health and sustainable agricultural production, and can achieve the effect of plant disease biological prevention and control.

The invention relates to application of exopolysaccharide in preparation of a product for promoting microbial colonization, and belongs to the technical field of microorganisms. The invention provides an application of exopolysaccharide in preparation of a product for promoting microbial colonization. The invention further provides application of the exopolysaccharide in preparation of products for preventing and treating bacterial wilt. Experiments prove that the exopolysaccharide separated from the paenibacillus mucilaginosus G78 has the effects of promoting colonization of the paenibacillus mucilaginosus in plant root systems and preventing and treating bacterial wilt, and meanwhile, the biomass of plants can be increased; the compound can be used as a plant prebiotic to be matched with beneficial bacteria to form a biopesticide to promote plant growth and prevent bacterial wilt, and can also be independently used as a biopesticide to be applied.

The invention discloses a method for repairing coal gangue soil based on CUE synthesized microbial communities, and belongs to the technical field of microorganisms. Charcoal or glucose is added into the coal gangue contaminated soil, and synthetic flora is inoculated for remediation, wherein the addition amount of the charcoal or the glucose added into the coal gangue contaminated soil is that 0.5-1.5% (m / m) charcoal or 15-25 ppm glucose is added into the contaminated soil containing 85-95% (m / m) coal gangue; the inoculum size of the synthetic flora is that 40-60 mL of bacterial liquid is applied to each kilogram of contaminated soil, and the content of the bacterial liquid is 1 * 10 < 7 >-3 * 10 < 7 > CFU / mL. According to the method, flora is synthesized based on CUE, a modifier (glucose / biochar) is utilized to the maximum extent, rapid planting and metabolism co-cultivation of the flora are achieved, it is ensured that strains can survive in a carbon-limited environment, downstream ecological functions such as nutrient circulation and community stress resistance are driven by means of the modifier, integration of microorganisms and physical and chemical means is achieved, and the method is suitable for large-scale popularization and application. The microbial colonization capability and the nutrient circulation efficiency are remarkably improved, the remediation efficiency is superior to that of a single modifier, and the bottleneck of carbon deficiency of the coal gangue tailings is solved.

The invention discloses a method and a system for synchronously detecting intracellular microbial colonization and host response at a single cell level, and belongs to the technical field of single cell epimics and microbiomics. Microorganism colonization information and host chromatin openness information of each single cell are synchronously extracted from single cell chromatin accessibility sequencing data, and correlation analysis is performed on the microorganism colonization information and the host chromatin openness information at the single cell level, so that a cell specificity mechanism of host-microorganism interaction is analyzed. At present, a systematic method capable of synchronously realizing microbiological detection and host chromatin accessibility analysis under single cell resolution is not reported yet, and the method fills the technical blank. The method can solve the problem that the existing sequencing technology based on 16S rRNA, 18S rRNA or scRNA-seq is difficult to analyze microorganism colonization and host chromatin accessibility synchronously and highly sensitively at the single cell level, and provides an innovative tool for revealing a host-microorganism interaction mechanism of cell specificity in complex tissues.

This invention discloses a novel carbon-based microbial organic soil conditioner, belonging to the field of soil improvement technology. It aims to solve the technical problems of existing conditioners, such as single-function effects, low microbial survival rates, poor long-term soil remediation effects, and insufficient nutrient utilization. The conditioner is composed of a modified carbon-based carrier, a composite functional microbial community, small-molecule organic carbon components, and synergistic ingredients in a specific ratio. This invention achieves a triple effect of improving soil physical structure, activating chemical nutrients, and regulating the biological microecology. It can improve microbial colonization and survival rates and nutrient utilization, passivate heavy metals and organic pollutants in the soil, enhance soil water and fertilizer retention capacity, realize the resource recycling of agricultural waste, and possess multiple values ​​of increasing yield and quality, soil remediation, and carbon sequestration. It is suitable for various farmland improvement and contaminated soil remediation scenarios.

This invention discloses a PHA-based barrier porous synergistic potted seedling cup suitable for natural composting, its preparation method, and its applications, belonging to the fields of biodegradable polymer materials, horticultural container structural engineering, and natural composting technology. Addressing the industry pain point that existing biodegradable containers struggle to balance wet-state durability with composting degradation speed, this solution innovatively constructs an asymmetric gradient structure with a loose outer layer and a hydrophilic inner layer. The outer layer employs highly efficient bio-based barrier technology, requiring only a very low addition amount to form a dense protective layer, achieving superior water control performance and structural strength comparable to petrochemical plastics. The inner layer features a specially designed hydrophilic porous system that actively absorbs water in the composting environment, triggering microporous disintegration, significantly improving microbial colonization efficiency and guiding root penetration, overcoming the technical barrier of difficult degradation of high-barrier materials. This product is fully biodegradable and fluorine-free and safe, perfectly achieving a balance between robust reliability during use and rapid return to nature after disposal, possessing broad application prospects.

The invention provides a microbial soil conditioner for in-situ rejuvenation of border trees. The soil conditioner comprises a chemical loosening conditioner (composed of modified sodium carboxymethyl cellulose and citric acid-malic acid composite organic acid), a biological stimulant (composed of bacillus subtilis exopolysaccharide and a seaweed extract) and a physical loosening auxiliary agent (composed of expanded perlite and crushed garden waste). Functional strains (bacillus subtilis and bacillus mucilaginosus) and a protective agent (food-grade sucrose fatty acid ester). According to the invention, a triple synergistic system of chemical loosening-physical supporting, biological stimulation-microbial colonization and nutrient activation-stress tolerance adaptation is constructed, the chemical conditioner rapidly disperses clay particles, the physical auxiliary agent constructs a stable pore skeleton, the biological components promote aggregate formation and microbial colonization, the soil porosity can be increased from less than 15% to 20% or more within 7 days, and the soil quality is improved. The conductivity is reduced to 1.8 mS / cm or below within 30 days, and the pH is adjusted to 6.5-7.5.

The invention discloses staphylococcus epidermidis NCU-07, a probiotic metagen and application, and relates to the technical field of biological medicine. The staphylococcus epidermidis NCU-07 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 18, 2023, and the preservation number is CGMCC NO.26493. The staphylococcus epidermidis NCU-07 is preserved in the China General Microbiological Culture Collection The bacterial strain is prepared into the probiotic metagen, and the application of the probiotic metagen, sulfadiazine silver cream and a compound coptis chinensis oil liniment in a medicine composition for treating skin burn wounds is provided in a combined mode of the probiotic metagen, the sulfadiazine silver cream and the compound coptis chinensis oil liniment. The probiotic metagen disclosed by the invention is beneficial to promoting the healing of the II-degree burn wound of a patient, can enhance the treatment effect when being combined with the sulfadiazine silver cream and the compound coptis oil liniment, relieves the itching symptom of the II-degree burn wound of the patient, has no obvious influence on the pain symptom of the wound, increases the diversity of skin florae, and can be used for treating the II-degree burn wound of the patient. Colonization and proliferation of pathogenic microorganisms can be inhibited, and proliferation of skin symbiotic bacteria is promoted.

The invention is directed to an aqueous extract of Eruca sativa (arugula) leaves that has antimicrobial activity on Gram-positive bacteria and mycoplasmas. The extract may be purified away from solid or insoluble components, standardized based on the weight of its non-aqueous or solid content, assayed for antimicrobial activity, and provided in an aseptic or sterile form for pharmaceutical use. It may be used to kill or inhibit the growth of microorganisms such as Gram positive bacteria, promote wound healing, or as prophylaxis against host colonization or infection by a microorganism.

The invention relates to the technical field of deep processing of tea leaves, and discloses a preparation process of medicinal and edible fermented tea, which sequentially comprises the steps of tea base prefabrication, medicine source enzymatic hydrolysate preparation, dietary fiber auxiliary material recovery, inoculated fermentation and gradient drying. The method is characterized in that a physical structure beneficial to microbial colonization is constructed by controlling the cell breakage rate of tea bases; a compound enzyme system containing cellulase, pectinase and beta-glucosidase is adopted for carrying out directional hydrolysis on raw materials such as rhizoma polygonati and radix puerariae, bitter glycoside is converted into sweet aglycone, and an ultramicro dietary fiber auxiliary material is prepared to serve as a fermentation carrier; after the eurotium cristatum is inoculated, the uniform growth of the golden flowers and the formation of flavor substances are promoted through two-stage temperature-control and humidity-control fermentation; and finally performing gradient drying to obtain a finished product. The process effectively solves the problems of few eurotium cristatum, heavy medicine flavor and rough taste of the traditional medicinal and edible fermented tea, realizes deep fusion of fungus fragrance and medicine fragrance tea fragrance, and has the advantages of flourishing eurotium cristatum, smooth soup feeling and stable quality.

The application relates to the technical field of environmental microbial remediation, and particularly discloses a composite microbial agent and application thereof in heavy metal pollution remediation. The composite microbial agent comprises a composite microbial bacteria and a composite carrier for immobilizing the composite microbial bacteria; the composite carrier is porous ceramic granules loaded with adsorption slurry, the adsorption slurry is prepared by mixing plant straw powder subjected to alkali dewaxing and a gelling agent solution; and the composite microbial bacteria are composed of Bacillus cereus and Pseudomonas fluorescens. The composite microbial agent of the application is prepared by mixing the straw subjected to alkali dewaxing and porous ceramic granules, so that the composite carrier capable of loading the microbial bacteria is obtained, and the composite microbial agent has microbial planting capacity and heavy metal remediation effect far exceeding those of a traditional simple mixed carrier.

The present invention discloses a microbial agent for preventing and treating tomato root rot, and belongs to the technical field of microbial materials. The present invention screens and obtains three biocontrol strains, Bacillus licheniformis, Bacillus subtilis and Monascus lunulatus, which have an inhibitory effect on a variety of pathogenic bacteria, especially a strong inhibitory effect on Fusarium oxysporum. The present invention also adds a small amount of composite nutrient solution composed of glucose, bean cake powder, etc. to provide sufficient nutrition supply for microbial activity. A microbial activity promoter composed of brown algae oligosaccharide and 5-aminolevulinic acid is added to improve the efficiency of microbial colonization. In actual tomato planting, it can effectively prevent and treat tomato root rot, promote the growth of tomato plants, improve tomato quality, and is green, safe and pollution-free, does not cause environmental pollution, can reduce the amount of other corresponding chemical pesticides, save expenses, reduce costs, has potential economic and environmental benefits, and is worthy of application and promotion.

Disclosed are adhesive medical films incorporating one or more cationic steroidal antimicrobial (CSA) compounds. The adhesive medical film is in the form of a tape, dressing, or sheet, configured for contact against dermal tissue to provide securement of medical devices, wound care, wound closure, and / or scar prevention. The adhesive medical film includes (i) a polymer adhesive (e.g., pressure-sensitive adhesive) in which one or more CSA compounds are distributed, and (ii) optionally, a backing substrate upon which the polymer adhesive is disposed. Examples of polymer adhesives include polyacrylate and silicone adhesives. The adhesive medical films, when used, reduce microbial colonization and / or growth, both on the films during storage and at a dermal site, as compared to the same adhesive medical film without incorporated CSA compounds.

This invention belongs to the field of biomedical technology, specifically relating to a set of microbial biomarkers for identifying adenoid hypertrophy and their applications. Based on the relationship between different microbial colonization patterns and the severity of adenoid hypertrophy, this invention utilizes abundance data from combinations of oral microbial biomarkers from different sample types to construct a model that can predict the severity of adenoid hypertrophy in patients. The predictive model constructed in this invention has an AUC value of 0.752, demonstrating good predictive performance. Combining model parameters and microbial characteristic information shows that the invented model is consistent with current clinical understanding, proving the reliability of the model.

The present application relates to a kind of based on piezoelectric effect reinforced permeability good oxygen biological reaction wall, piezoelectric material is filled as filler to be filled in permeable biological reaction wall body, the pulse hydraulic pressure produced by the flow field potential energy formed by groundwater extraction is used, the deformation of the surface of piezoelectric material occurs and generates electric charge and forms local electric potential field, stimulate microbial activity, to improve microbial colonization and pollutant degradation capacity.Permeable biological reaction wall is provided with the baffle of hollow fiber membrane assembly, and through super micro bubble aeration, the in-situ aerobic metabolic efficiency of microorganism is increased, the release of volatile and semi-volatile organic pollutants in groundwater is avoided to escape and scatter and influence surrounding environment.The present application realizes long-term efficient groundwater composite organic pollutants green low-carbon sustainable biological process control, significantly improves groundwater composite organic pollution repair efficiency, avoids secondary pollution and repair tailing rebound phenomenon, and realizes long-term groundwater pollution risk management and control.

The invention belongs to the technical field of strawberry planting, and relates to a high-performance composite soil conditioner for rapidly improving continuous cropping obstacles of strawberries and a preparation method of the high-performance composite soil conditioner. The conditioner comprises a humic acid-earthworm mixture, coated modified biochar, a modified mineral material, seaweed residues, oyster shell powder, a calcium magnesium phosphate fertilizer and a natural binder. The humic acid is activated through the earthworm extracting solution, so that the biological activity is improved; beneficial bacteria are deeply loaded in pores of the biochar by adopting a vacuum decompression technology, so that the colonization capability of microorganisms is enhanced; mineral materials are activated by citric acid, so that the adsorption capacity on pathogenic bacteria and heavy metals is improved; and finally, the lasting release of the active ingredients is realized through a coating slow-release technology. The conditioner can effectively improve soil micro-ecology, effectively inhibit soil-borne diseases, regulate soil pH and enhance nutrient supply, has the advantages of high efficiency, safety and durability, and is suitable for rapid treatment of strawberry continuous cropping obstacles and soil health restoration.