280 results about "Bacterium nitrobacter" patented technology

The invention discloses a large-scaled culture method of high-concentration nitrosobacteria and application thereof. The method comprises the following steps of: (1) adding an enrichment culture solution with initial ammonia nitrogen mass concentration of 30-500mg/L in a reaction device with functions of stirring, heating and aeration; preparing activated sludge of a waste water treatment plant in activated sludge of 0.5-20% for primary inoculation to conduct the enrichment culture of the nitrosobacteria by 2-6d as a culture cycle, wherein extra ammonium salts and a growth promoter are added during the enrichment culture of the nitrosobacteria; (2) ending the culture when the concentration of NO2-N in the solution is accumulated to reach 70-95% of the concentration of total nitrogen; adding a flocculating agent for flocculation and sedimentation; and allowing to stand still to remove supernate, and then adding the enrichment culture solution to start the culture of the second cycle; and (3) continuously conducting 3-8 cycles of the enrichment culture to obtain the high-concentration nitrosobacteria. By adopting the nitrosobacteria to strengthen the bi-nitrification treatment of ammonia nitrogen contained waste water, the method has the advantages of high stress resistance, low cost and high efficiency and is suitable for the complicated treatment of ammonia nitrogen contained industrial waste water.

The invention discloses a enrichment method of nitrifying organism. The method adapts discontinuous activated sludge process by gradually improving ammonia nitrogen ph indicator in nutrient fluid to enrich. The main component of the enrichment nutrient fluid is inorganic salt comprising microelement Fe, Mg, Na, K and amortization liquor, in which inceptive concentration of NH(4)(+-N) is 100mg/L to 200mg/L, final concentration is 500mg/L to 1200mg/L and COD is less than or equal to 200mg/L. The method can restrain evidence the growth of sundry bacterium such as carbonizing bacterium, is propitious for nitrifying organism to become ascendency bacterium and resist more and more high ammonia nitrogen concentration, and finally disposing concentration of the ammonia nitrogen wasted water is up to 1200mg/L and high concentration ammonia nitrogen in wasted water can be reduce to less 10mg/L.

The invention relates to a compound microecological preparation used to purify aquaculture water in the field of aquiculture, a preparation form and a preparation process thereof; the preparation is composed of photosynthetic bacterium, bacillus subtilis, denitrifying bacterium, coagulant aid and auxiliary components; the preparation form is composed of an inner layer and an outer layer, wherein, the outer layer comprises bacillus subtilis, denitrifying bacterium and coagulant aid, and the inner layer comprises solid photosynthetic bacterium particles. The invention breaks through the prior theory that people keeps using nitrite bacteria and nitrobacteria to solve the problem of the accumulation of ammonia nitrogen and nitrite in the aquaculture water in the control of aquaculture water, introduces an advanced SHARON theory in wastewater treatment, integrates the characteristics of the aquaculture water quality, purifies the aquaculture water with denitrifying bacteria strains as the main bacteria strains, can quickly and effectively reduce the content of NH3 and NO2- in the aquaculture water, is not inhibited by the concentration of the NO2-, and effectively reduces the oxygen consumption in the aquaculture water.

The invention describes application of pure culture microbial strains under denitrifying conditions for growth on crude oil thereby modifying crude oil properties to enhance its recovery.

The invention relates to a microbial agent for purifying river wastewater and a preparation method of the microbial agent. The microbial agent comprises the following components in parts by weight: 20-40 parts of bacillus natto, 10-30 parts of nitrobacteria, 5-15 parts of white-rot fungi, 5-15 parts of pseudomonas and 10-40 parts of photosynthetic bacteria. The microbial agent is well-adapted and has the capabilities of fast forming dominant bacterial communities and replenishing indigenous rhizobia to the water body; and the microbial agent can be used for reducing the COD (Chemical Oxygen Demand), NH3-H, total nitrogen, TP (Total Phosphorus) and the like, removing odor and color and producing high-activity bio-enzyme to suppress the growth of harmful bacteria and acceleratively decompose residual baits as well as animal and plant residues, and a remarkable water quality purifying effect is achieved.

The invention discloses a microorganism concrete brick and a preparation method thereof. The microorganism concrete brick is formed by mixing cement, gravel, sawdust (or straw scraps, scraps of paper, and the like), mixing mixed microorganism with water according to a certain proportion, forming by a mold and then naturally hardening, wherein the mixed microorganism comprises six floras including bacillus, nitrobacteria, photosynthetic bacteria, saccharomycetes, lactobacillus and actinomycetes. The microorganism concrete brick has excellent water quality purifying function, can continuously play roles for a long time, and is widely applicable to purify various water qualities such as rivers, lakes, canal water, swimming pools, fishponds and the like.

The invention provides a microbe-enzyme composite preparation used for restoring water in an urban and rural polluted river, and its preparation method. The urban and rural polluted river restoration microbe-enzyme composite preparation is characterized in that the microbe mainly comprises 30-60% of Bacillus licheniformis, 25-35% of a nitrobacterium, and 15-35% of pseudomonas putida; and the composite enzyme mainly comprises 40-60% of a protease, 15-25% of an amylase and 25-35% of a cellulase. The optimized microbe is compounded with the composite enzyme to prepare the microbe-enzyme composite preparation against water of different pollution degrees, the addition amount of the microbe is 1-5g/m<3>, and the addition amount of the composite enzyme is 0.01-0.05g/m<3>. The microbe-enzyme composite preparation is added to the urban and rural polluted river to carry out microbial reinforced restoration with aeration as an auxiliary measure, can effectively improve defects of low microbe metabolism activity, low environmental tolerance, short retention time in the polluted water and the like existing in the prior art, and has a substantial water purifying effect.

The invention provides a microbial carrier. The microbial carrier is in a porous solid form and contains a biodegradation material with the weight content of 50% or more, the specific surface area is 100-900 m<2>/g, the average pore size is 20 nm-0.4 mm, the microbial carrier is at least of a two-layer structure, and the average pore size of the surface layer is 50 nm-0.4 mm. The microbial carrier can provide larger living environment for microorganisms, provide a carbon source for growth and propagation of microorganisms particularly denitrifying bacteria, improve the concentration of microorganisms in unit volume and improve the microbiological treatment efficiency.

The invention belongs to the applied biology technical field, and relates to a water environmental microbiology reparation agent and a preparation method thereof. The reparation agent has the mass percent of the components in the microbiology total weight as follows: bacillus subtilis is 30-40 percent, bacillus licheniformis is 30-40 percent, ray fungi is 10-20 percent, denitrifying bacterium is 10-20 percent, and photosynthetic bacterium is 10-20 percent. The microbiology preparation of the invention has strong adaptability, can compensate the water body original bacterium when being put in water, quickly form the preponderant flora in the water body, produce the high-activity biological enzymes for quickening the decomposition of the left diet and the animal wastes, and produce the antibiotic substance to restrain the growing of harmful bacterium and reduce the occurrence of the illness as well as ensure the production of green marine products.

The invention relates to a fertilizer synergist and application thereof, belongs to the field of a fertilizer, and aims at solving the technical problem, namely providing an efficient, safe and inexpensive fertilizer synergist. The fertilizer synergist disclosed by the invention comprises the following active components: 1-10 parts of polymerization amino acid and 0.5-2 parts of nitrification inhibitor, wherein the nitrification inhibitor is a compound with inhibiting effects on nitrobacteria and denitrifying enzyme in soil. Furthermore, the invention also discloses a preparation method and application of the fertilizer synergist. The polymerization amino acid in the active components of the fertilizer synergist disclosed by the invention is a polymer biopolymer of amino acid. Thus, the fertilizer synergist is easily biodegraded, and safe to the soil and environment, and has the advantages of being efficient, safe and inexpensive.

The present invention relates to one kind of functional biological carbon fiber and its preparation process and application as biomembrane carrier. The biomembrane carrier is prepared with polyacrylonitrile carbon fiber, and through electrochemical surface treatment in 5-20 % concentration ammonium bicarbonate electrolyte to increase the surface acid oxygen containing functional groups and raise wettability, and gluing with water soluble epoxy resin in the amount 0.5-2 %. The biomembrane carrier can fix nitrobacteria, sulfate reducing bacteria and anaerobic methanogen. It may be used in treating high concentration acrylon waste water, and can eliminate sulfate, COD and ammonia nitrogen effectively. In addition, the biomembrane carrier is easy to regenerate and has no secondary pollution.

The invention relates to a method for preparing a composite heterotrophic nitrobacteria water quality modifying solid powder or granule, and belongs to the technical field of aquiculture technology and ecological environmental protection. The water quality modifying solid powder and granule is prepared by the following steps: mixing Bacillus foecalis alkaligenes CGMCC NO.1.1786 and arthrobacterium CGMCC NO.1282 into mixed bacteria; performing fermentation on the mixed bacteria for 24 to 36 hours in a culture medium in a fermenter at the fermentation temperature of between 28 and 32 DEG C; processing the obtained product into a solid powder or granule after fermentation culture; and preparing the composite heterotrophic nitrobacteria water quality modifying solid powder or granule with the functions of reducing ammonia nitrogen and nitrite nitrogen in a water body. The preparation is convenient to use, is slightly influenced by environmental conditions, has small dosage, can obviously reduce the content of the ammonia nitrogen and the nitrite nitrogen in the pool water body after use, maintains the effect for 10 to 15 days, and can effectively prevent diseases and paroxysmal death of aquaculture organisms and improve the yield and the quality of the aquaculture organisms when simultaneously improving the environmental quality of the pool water quality and the survival conditions of the aquaculture organisms.

The invention discloses a method for denitrifying wastewater generated in the process of producing an FCC (fluid catalytic cracking) catalyst. The method comprises the step of adding nitrobacteria and nitrous acid denitrifiers to sewage, wherein the ammonia nitrogen containing sewage treatment temperature is 18-40 DEG C; the dissolved oxygen is 0.2-3mg/L; and the pH value is 7.5-8.5. The method adopts autotrophic bacteria and heterotrophic bacteria which are subjected to combination of superiority to serve as reinforced microorganisms for treating wastewater, adopts different addition modes, can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, truly achieves short-cut nitrification and denitrification or simultaneous nitrification and denitrification of the ammonia-containing wastewater generated by producing the catalyst by being combined with high-efficiency devices, and achieves up-to-standard discharge of pollutants such as ammonia nitrogen and the like in the wastewater.

The invention provides a method for treating waste water with high pH value, high sulfide content, high ammonia and nitrogen content and high COD content. The method comprises the following steps: firstly, pretreating the waste water by adopting acidification-malysite flocculation sedimentation to reduce the concentration of S2- in the waste water, eliminate the restraining function of the S2- to biological treatment, remove COD caused by the S2- and decompose substances which are easily acidized and decomposed in the waste water; secondly, carrying out biological treatment, wherein the biological treatment consists of processes of hydrolytic acidification and autotrophy SBR, organisms which are difficultly decomposed are decomposed in the hydrolytic acidification process to provide a carbon source for denitrification, oxygen is provided by aeration for the autotrophy SBR, the COD in the waste water is removed by using aerobic microorganisms, NaHCO3 is additionally added to adjust the pH value to ensure an optimal growth environment for autotrophy nitrobacteria, and ammonia and nitrogen are oxidized and removed by using oxygen and HCO32-; and finally, carrying out deep treatment by adopting flocculation sedimentation and -ClO2 oxidation to remove the color of the waste water and ensure that all indexes achieve the discharge standard.

The invention provides a microorganism dredging method. The microorganism dredging method is characterized in that a microbial preparation is implanted in sludge at the bottom of a river channel, the implanting position is 10-15cm far away from the upper layer of the sludge, and the microbial preparation is dredging bacteria which takes porous zeolite particles as carriers, is added with peroxide and contains live bacteria including bacillus subtilis, desulfurizing microorganisms H1-H4, phosphorus accumulation organisms, nitrifying bacteria and denitrifying bacteria which are in proportion. A dredging device comprises a fixed support, a rack and a microorganism implantation groove, wherein the bottom of the rack is provided with a bottom flat plate with a hole, a feed port is formed in the upper part of the microorganism implantation groove, a discharge port is formed in the lower part of the microorganism implantation groove, and an implantation mechanism is arranged in the microorganism implantation groove. The dredging device is used for implanting the microbial preparation in the sludge at the bottom of the river channel, so that microorganisms realize aerobiotic and anaerobic metabolism in anaerobic and facultative aerobic environments and transform matters such as sulfide, nitrite and organic matters in the sludge through microorganism cell metabolism, thus a biological dredging effect is achieved.

The invention discloses a biological deodorant. The biological deodorant is characterized by comprising the following raw materials in percentage by mass: 20%-30% of a complex microbial agent, 40%-50% of organic acid and 30%-40% of calcium superphosphate, wherein the complex microbial agent comprises saccharomyces cerevisiae, bacillus subtilis, germany lactic acid bacteria, nitrobacteria, thiobacillus thiooxidans, acetobacter oxydans and an adsorption material; and the organic acid is selected from one or more of oleic acid, linoleic acid, linolenic acid, arachidonic acid, polybasic carboxylic acid, oxalic acid, malic acid, amino acid, sulfoacid and sulfinic acid. According to the biological deodorant, the original foul gas in garbage can be quickly and effectively adsorbed, removed and covered, the work environment of a worker in a composting plant is improved, composting and stabilizing of organic garbage are accelerated, and novel foul gases such as ammonia and hydrogen sulfide generated and released in the composting process are prevented from being reduced.

The invention discloses a biological denitrification process of organic nitrogen waste water, and belongs to the technical field of waste water denitrification. The biological denitrification process comprises the following steps: (1) pre-treating the organic matter waste water, and then sending the pretreated organic matter waste water into an anaerobic fluidized bed at a given speed; (2) facilitating the waste water to flow by the anaerobic fluidized bed having heterotrophic bacteria, converting organic nitrogen to be ammonia nitrogen, and collecting the rest organic matters by utilizing a membrane; (3) enabling the waste water to enter a two-layer micro-organism membrane reaction tank, carrying out nitrous-acid nitration reaction by virtue of a first layer of microorganism membrane with nitrous-acid nitration bacteria, to generate nitrous acid; and carrying out the denitrification by virtue of a second layer of microorganism membrane with anaerobic ammoxidation bacteria. The entire process flow is reasonable in design, the operation condition is easy to implement, the cost is low, the denitrification rate is extremely high, and the biological denitrification process is suitable for continuous mass treatment on the organic matter waste water.

The invention relates to enrichment of a low temperature-resistant heterotrophic nitrification-aerobic/anoxic denitrification microbial agent and application thereof in wastewater treatment under an anoxic condition. The microbial agent is capable of realizing simultaneous nitrification and denitrification in an aerobic or anoxic environment, in the presence of both ammonia nitrogen and nitrate, with sodium acetate as a unique organic carbon source under a 10 DEG C low-temperature condition; therefore, inorganic nitrogen in wastewater is transformed into gaseous nitrogen and removal of total nitrogen is realized. The low temperature-resistant microbial agent thoroughly realizes maintenance and stabilization of activity of aerobic nitrification and heterotrophic denitrification bacteria under the low-temperature condition, and also has a very high total nitrogen removal rate under an anoxic condition. Besides, the heterotrophic microbial agent grows quickly and lays a good foundation for quick amplification and actual application. When applied to practical town sewage treatment, the microbial agent is capable of efficiently removing total nitrogen in the low-temperature environment under the single anoxic condition; and therefore, the problem that a traditional treating plant hardly reaches the national class A emission standard of total nitrogen in winter is solved and the utilization potentiality of the microbial agent is huge.

The invention discloses a composite micro-ecological preparation for treating black stink water in rivers, pits and ponds and a preparation method thereof. The composite microbial preparation comprises indigenous microorganism, functional strain and carrier matrix which are separated from a black stink water body to be treated and have a repairing function. The indigenous microorganism is at leastone of nitrifying bacteria, denitrifying bacteria and bacillus subtilis, the functional strain is at least one of photosynthetic bacteria, candida utilis, bacillus megaterium, lactic acid bacteria and actinomycetes, and the carrier matrix is at least one of crocalite, green zeolite, ceramsite, quartz sand, vesuvianite and vermiculite. The black stink water body includes black stink surface waterof black stink rivers, black stink lakes or polluted ground. According to the invention, the composite micro-ecological preparation is formed by immobilizing the indigenous microorganism, the functional strain and the carrier matrix, has remarkable effects of degrading the pollutants such as COD, NH3-N, TP and the like in the black stink water. The preparation method disclosed by the invention haswide applicability to black stink surface water of various black stink rivers, black stink lakes or polluted ground.

The invention belongs to the technical field of biological engineering and environmental engineering and discloses a low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03. The strain is suitable for growing in a neutral culture medium at the pH (potential of hydrogen) of 7.2 and at the temperature of 10 DEG C, and is characterized in that the strain is atrichous, aerobic and capable of achieving denitrification, has yellowish-white cells and can grow with nitrate nitrogen serving as energy, and both catalase and oxidase are positive. The strain obtained by screening solves the problems of low production efficiency, low denitrification efficiency in winter and the like during sewage treatment of northern cold cities, removal rate of TN (total nitrogen) in low-temperature domestic sewage reaches 74%, and on the premise of guaranteeing stable denitrification efficiency, denitrification rate of urban sewage treatment systems in winter is increased, production efficiency is improved, production energy consumption is reduced, and the stain is of great significance to realization of short-range synchronous nitrification and denitrification.

The invention discloses a starting method for rapid and efficient short-cut nitrification. The method comprises the following steps: guiding inoculated sludge which is rich in nitrobacteria into an SBR (Sequencing Batch Reactor) reactor; guiding sewage into the SBR reactor in order that the concentration of the sludge in the reactor becomes 2,500-3,500mg/L, and controlling the temperature of the sewage at 30+/-1 DEG C and the pH of the sewage at 7.9-8.2; starting an aeration facility in the reactor in order that the concentration of dissolved oxygen becomes 0.8-1.2mg/L; performing aeration for 1.5-2.5 hours; stopping aeration, and starting to stir for 0.5-1.5 hours to uniformly mix the sludge and the sewage; repeatedly aerating and stirring operation for 1-5 times; standing the mixture for separating a solid from a liquid; and draining supernatant. By performing the short-cut nitrification in an intermittent aeration way, the growth of AOB (Ammonia Oxidizing Bacteria) can be better facilitated, a nitrification reaction is restrained, the energy consumption of a reaction device can be lowered effectively, and the starting of the short-cut nitrification of domestic sewage with low ammonia nitrogen content can be realized in comparison to the conventional continuous aeration.

The invention provides a novel EM fungicide for composting dung. The novel EM fungicide is obtained by mixing effective microorganisms, an organic matter biodegradation acceleration factor and a heavy metal binding material at a mass ratio of (1-2):(3-5):(4-6); the effective microorganisms are obtained by mixing photosynthetic bacteria, Lactobacillus, Bacillus, Saccharomyces, white rot fungi, actinomycetes, nitrogen-fixing bacteria, trichoderma and nitrobacteria at a mass ratio; the organic matter biodegradation acceleration factor is composed of inorganic salt, cellulose enzyme enharpin, water-soluble sugars and organic acid; the heavy metal binding material is a bentonite molecular sieve or coal ash molecular sieve. The novel EM fungicide can be used for simultaneously solving the problems of bad smell pollution, heavy metal pollution and auxiliary material shortage in a dung composting process.

The invention discloses a biomimetric eco-pond system for the deep treatment of wastewater from breeding. A treatment method comprises a step that after being subjected to anaerobic digestion treatment, wastewater from breeding first enters a water distribution tank for accumulation and water distribution, then enters a sank filter tank from a perforation on the bottom of the water distribution tank and finally enters a pond body after passing through a water distribution aeration slope for short-range purification, wherein the bottom of the pond body is provided with artificial float grass. A treatment system comprises the water distribution tank, the sand filter tank, the water distribution aeration slope, the pond body, an artificial float grass belt, water plants and a floating body and mud collecting well, wherein the water plants on the surface of the water in a pond are planted on floating bodies, so that in the growth process, the water plants can fully absorb carbon, nitrogen and phosphor in the wastewater and conveys oxygen from the leaves of the plants to the roots of the plants through the respiration of the plants to form a micro aerobic environment at a root part for further simulating the decomposition of organic substances and the growth of nitrobacteria, thereby removing nutrients such as nitrogen and phosphor in the wastewater and improving the overall aesthetic value of the pond body.

The invention provides a microorganism bacterium agent for subtracting organic sludge. The microorganism bacterium agent is characterized in that: the microorganism bacterium agent per milliliter contains more than 2.7*10<9> of live bacteria, and the pH value is 3-4; the microorganism bacterium agent comprises photosynthetic bacteria, bacillus mucilaginosus, lactic acid bacteria, fusarium, anaerobic clostridium, denitrifying bacteria, sphaerotilus and zymogenous filamentous fungus. The invention also provides a corresponding production method of the microorganism bacterium agent and a complex culture medium. According to the invention, a mode of combining a plurality of floras is adopted to reduce and degrade the organic sludge and organic pollutants in water in a river (lake) or a municipal sewage treatment plant, and a limit that the effect is reduced due to single strain variation easily caused by environment change is avoided.

The invention discloses a nitrobacterium continuous production method and production equipment thereof. The production equipment comprises a culture tank, a nutrient solution conveying system, an oxygen conveying system, a water inlet pipe, an alkalinity adjusting alkali supplementing tank, a concentrated supplementing tank without activated carbon, a make-up pump, a drainage pump, a bacterium liquid drainage pump and a membrane assembly in the culture tank. The nutrient solution conveying system comprises a nutrient solution storage tank, a nutrient solution pump and a nutrient solution conveying pipe, and the nutrient solution storage tank is connected with the nutrient solution pump through the nutrient solution conveying pipe. The oxygen conveying system comprises a fan and an inverted-umbrella-shaped aeration head, an output pipe of the fan is introduced from the lower portion of the culture tank to be connected with the inverted-umbrella-shaped aeration head, and the output end of the nutrient solution conveying pipe, an output pipe of the concentrated supplementing tank without the activated carbon, the water inlet pipe and a drainage pipe of the alkalinity adjusting alkali supplementing tank are mounted above the culture tank. The activity of the obtained product has quantitative indexes, and finally the product reaches 500 mgN/Lbacterium liquid/h. Thalli and a nutrient solution can be separated more efficiently.