84 results about "Bacterium nitrobacter" patented technology

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses a filler reinforced ecological floating bed for purifying aquaculture tail water and application thereof, and belongs to the technical field of water treatment. The filler reinforced ecological floating bed comprises canna cultivated in a floating plate and straw or straw biochar suspended below the floating plate; the straw biochar is obtained by taking straw as a raw material and pyrolyzing the straw at 500 DEG C or 700 DEG C. The ecological floating bed capable of purifying the aquaculture tail water is prepared by utilizing the emergent aquatic plant canna which has high purification efficiency on nitrogen and phosphorus and the straw or straw biochar which has a large surface area and is beneficial to the growth of denitrifying bacteria, and the ecological floating bed can effectively reduce the content of nitrogen and phosphorus in the aquaculture tail water, accelerate the denitrification and dephosphorization rate and improve the quality of the aquaculture tail water. The tail water purification method can be used for reducing nitrate accumulation, realizing tail water purification, providing scientific basis and technical support for tail water treatment work in aquaculture, and being beneficial to promoting green and sustainable development of aquaculture.

The invention provides a compound microbial agent and application thereof in regulating aquaculture water quality, and the compound microbial agent is prepared by the following steps: firstly, embedding a bacterial liquid composed of alcaligenes faecalis, pseudomonas, ochrobactrum and sphingosine box bacteria through self-assembly by adopting gallic acyl quinic acid, and then modifying lignin by using ferrocene for composite loading. And immersing into a bacterial liquid consisting of photosynthetic bacteria, bacillus and nitrifying bacteria, and drying. According to the invention, ferrocene modified lignin and galloylquinic acid construct a multi-layer load structure to fix microorganisms in a layered manner, so that the microorganisms can stably survive in a complex water body environment, and meanwhile, a plurality of florae act synergistically to degrade organic matters, nitrogen, phosphorus, antibiotics and the like in aquaculture water, so that the degradation efficiency of water pollutants can be remarkably improved; effective adjustment of aquaculture water quality is facilitated, and good data support and theoretical basis are provided for water body improvement in the field of aquatic products.

The invention discloses a bottom mud ecological restoration material as well as a preparation method and application thereof. The material is composed of a degradable carrier, a treatment flora and a multifunctional filler. The carrier is porous particles die-cast by corn straws and the like; the treatment flora comprises photosynthetic bacteria, bacillus and nitrifying bacteria; the filler is prepared from non-nitrate type hydrotalcite-like compounds, calcium nitrate, nitrate type hydrotalcite-like compounds and a flocculating agent according to a specific mass ratio. The preparation method comprises the following steps: carrying out pressure casting on the carrier, adsorbing the flora, and rolling the filler to form balls. The restoration method comprises the steps that a river channel is cut off in the dry season to form a closed area, materials are put according to the area ratio of 10: 1 for preliminary restoration, and after standing is conducted for 1-5 days, the materials are put along with water flow according to the area ratio of 2: 1 for deep restoration. Through the physical, chemical and biological synergistic effect, the sediment redox environment can be effectively improved, pollutant release and degradation are promoted, operation is easy and convenient, and the risk of secondary pollution is low.

The present invention relates to a salt-tolerant heterotrophic nitrifying and denitrifying bacterial strain, its cultivation method, and application. The strain addresses the technical problem of existing nitrifying and denitrifying bacteria inhibiting nitrification in the presence of a carbon source. The strain is named Pseudomonas stutzeri YXH-102 and is deposited with the General Microbiology Center of the China National Center for Microbiological Culture Collection under the accession number CGMCC No. 27688. The present invention can be used in the field of sewage denitrification treatment.

The application discloses an ecological purification and restoration agent for eutrophication landscape water body and a restoration method, and belongs to the technical field of water, waste water, sewage or sludge treatment. The ecological purification and restoration agent for eutrophication landscape water body comprises the following components in parts by weight: 2.4-5.5 parts of a composite microbial agent, 3.5-6.0 parts of a porous functional polymer material, 12-16 parts of bentonite, and 18-30 parts of clinoptilolite. The porous functional polymer material is adopted to reduce the content of nitrogen and phosphorus in a short time through chemical adsorption, and the composite microbial agent prepared according to a specific proportion of bacillus subtilis, bacillus amyloliquefaciens, pseudomonas denitrificans and nitrobacter winogradskyi is used to comprehensively restore the water body, so that the eutrophication of the water body is effectively relieved, and the cost is low and the purification is complete.

The invention relates to the technical field of microbial fermentation, and particularly discloses a high-density expanding culture method of autotrophic nitrifying bacteria. The method comprises the following steps: screening low-temperature aquatic plants, and carrying out stepped pressurization domestication to obtain low-temperature-resistant and high-salt-resistant autotrophic nitrobacteria strains; a three-stage stepped expanding culture process is adopted, and a carrier filler with a high specific surface area is added in second-stage fermentation and third-stage fermentation; in the fermentation process, continuous collaborative fed-batch feeding, pH linkage regulation and control and dissolved oxygen precise linkage control of an inorganic carbon and nitrogen source are implemented. Through stress-resistant strain screening, carrier adding and precise process control, the density of the thalli in the fermentation liquor can be increased, the stress resistance of the thalli is improved, and the method is suitable for a more complex water body environment.

[Problem] To provide a denitrification filter material in which the form thereof is maintained without collapsing and which is digested only when fed on by denitrifying bacteria. [Solution] A denitrification filter material according to the present invention is configured from a biodegradable melt-kneaded resin composition containing (A) a water-insoluble and / or non-seawater-soluble biodegradable resin component and (B) a biomass polysaccharide polymer component. The component (A) is preferably an ester-bonded polymer, and is more preferably one or more selected from polybutylene succinate adipate, polyhydroxyalkanoate, and polycaprolactone. The component (B) preferably contains a biomass polysaccharide polymer component having a helical molecular structure. A water purification device comprises: a denitrification tank to which water being treated is supplied; the aforementioned denitrification filter material, which is accommodated in the denitrification tank and fixes aerobic denitrifying bacteria for reducing nitrate nitrogen in the water being treated; and an air supply mechanism that supplies air to the water being treated.

The utility model discloses a blowdown pipeline of a saturation tower. The blowdown pipeline comprises a main pipeline, and a front-end filtering heat exchanger, a plate heat exchanger and a sewage treatment tank which are sequentially arranged from upstream to downstream, the front-end filtering heat exchanger comprises a shell and a plurality of heat absorption layers, each heat absorption layer comprises a plurality of inverted-V-shaped hollow plate bodies which are evenly distributed in the horizontal direction, and the hollow plate bodies of every two adjacent heat absorption layers are distributed in a staggered mode. The shell is also provided with a sewage inlet, a sewage outlet, an impurity outlet, a water replenishing port, a water dividing tank and a water collecting tank, the impurity outlet is provided with a discharge pipe, and the downstream end of the discharge pipe is connected with the sewage treatment tank; two ends of the hollow plate body are respectively communicated with the water distribution tank and the water collection tank; the temperature of sewage entering the plate heat exchanger is reduced in advance through the front-end filtering heat exchanger, so that abnormal temperature rise of the subsequent sewage treatment tank is avoided, and nitrobacteria culture of the sewage treatment tank is facilitated; in addition, the front-end filtering heat exchanger also realizes coarse filtration and precipitation of sewage, so that the blockage problem of the plate heat exchanger is effectively reduced.

The invention relates to a water regulating microbial preparation for breeding largemouth bass seedlings in a land-based round pond and application, and belongs to the technical field of aquaculture. The preparation aims to solve the problem of combined pollution caused by synchronous exceeding of ammonia nitrogen, nitrite and hydrogen sulfide in a water body due to accumulation of residual feed and excrement in a land-based round pool high-density breeding environment. According to the scheme, the microbial agent is prepared by physically mixing bacillus standard bacterial powder, photosynthetic bacteria standard bacterial powder, compound nitrifying bacteria standard bacterial powder and thiobacillus standard bacterial powder according to specific parts by weight, wherein the bacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis, and the thiobacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis. The composite nitrifying bacteria standard bacterial powder is formed by mixing nitrite bacteria and nitrifying bacteria, and the thiobacillus thiooxidans standard bacterial powder is formed by mixing thiobacillus thiooxidans and thiobacillus thioparus. The preparation is used for synchronously degrading ammonia nitrogen, nitrite and hydrogen sulfide in aquaculture water, so that normal development of gonads of largemouth bass parents is guaranteed, and the hatching rate of fertilized eggs and the survival rate of early-stage seedlings are increased.

The application discloses a closed fishery circulating aquaculture system, which comprises an aquaculture device, a water circulation pipeline, a biochemical device, an oxygen supply device and a gas supply device. The aquaculture device comprises an aquaculture tank and a feeding assembly. The aquaculture tank is provided with an aquaculture cavity. The aquaculture tank is provided with a first water inlet pipe and a first water outlet pipe. The feeding assembly is arranged on the aquaculture tank. The water circulation pipeline is arranged between the first water inlet pipe and the first water outlet pipe. The biochemical device comprises a biochemical tank. The biochemical tank is provided with a biochemical cavity. The biochemical cavity is communicated with the water circulation pipeline. The biochemical cavity is used for accommodating nitrifying bacteria. The oxygen supply device is connected with the aquaculture tank and the biochemical tank. The gas supply device is provided with a gas conveying pipe between the aquaculture tank. The closed fishery circulating aquaculture system can not only meet the pressure requirement of aquatic products growing in different environmental pressures, but also make the water dissolving oxygen efficiency in the aquaculture cavity higher and be more beneficial to the growth of the aquatic products. In addition, the biochemical device is more beneficial to the growth of the photophobic and aerobic nitrifying bacteria.

The application discloses a preparation method of a porous sulfur mineral autotrophic denitrification material and application of the material in black and odorous water purification. The porous sulfur mineral functional material is prepared by using coral, iron-manganese oxide minerals and sulfur minerals, so that waste is reused, resources are saved, and the preparation method is simple; on the other hand, the porous sulfur mineral functional material has a large specific surface area and high activity. The high-activity porous sulfur mineral functional material can be used as an electron donor of sulfur autotrophic denitrification bacteria and denitrifying sulfur bacteria in a black and odorous water body with a low carbon-nitrogen ratio. Meanwhile, the coral, iron-manganese oxide minerals, nano zero-valent calcium and nano zero-valent iron-manganese formed by hydrogen reduction roasting, and the hydrated silicate formed by the sulfur mineral and cement improve the alkalinity in the reaction system, can neutralize the sulfur autotrophic denitrification acid production process, and realize deep purification of the black and odorous water body.

A strain of antibiotic-resistant marine heterotrophic ammonia assimilation-aerobic denitrifying bacteria and its application, belongs to the field of water treatment of aquaculture. The seawater antibiotic-resistant high-efficiency nitrogen removal Marinobacter nitratireducens LAN01 can use polyhydroxyalkanoate as the sole carbon source, and can realize heterotrophic ammonia assimilation and aerobic denitrification function under the stress of different concentrations of sulfamethoxazole antibiotics, and simultaneously transform phosphate. The strain has multiple resistances such as high salinity, high alkalinity and antibiotic, and can be used for seawater aquaculture effluent treatment, seawater recirculating aquaculture water treatment, high-salinity wastewater treatment and saline-alkali soil bioremediation, etc. To realize the simultaneous recovery of nitrogen, phosphorus and other nutrients in the seawater aquaculture environment.

The utility model discloses a sinking type landscape fish pond water circulation system which comprises a sinking type water pond, a sewage bin, an oxygenation pipe and a sewage pump, and the edge of the sinking type water pond is fixedly connected with a steel concrete pond wall. The corridor and the waterside rest tea pavilion are built beside the sunken pool, and the rockery, the water well and the high-low step flower bed are built on the periphery of the sunken pool, so that rich natural landscapes are achieved, and aesthetic fatigue is prevented; the clean water pump inputs clean water into the sunken water pool, and water in the pool is discharged; impurities in the pool are conveyed to the sedimentation bin, the rattan cotton bin, the biochemical bin and the biochemical sterilization bin through the sedimentation water pipe to be filtered and sterilized, sewage in all the bins is conveyed to the sewage bin through the sewage discharge pipe to be discharged uniformly, circulation and filtration of pool water are completed, flowing water can prevent inorganic salt from being deposited, and then the purpose of purifying water is achieved. Nitrobacteria are fed into the biochemical sterilization bin, so that breeding of pathogenic bacteria in water is effectively inhibited, the problem of pond water deterioration is relieved, and the water is kept clear.

The present application belongs to the technical field of water pollution treatment, and relates to microbial treatment, in particular to an aged iron filings promoted aerobic iron-reducing fungi for denitrification and application thereof. Aspergillus The aerobic iron-reducing fungi is classified and named as sp. R4, and is preserved in China Center for Type Culture Collection on February 13, 2025, with a preservation number of CCTCC NO: M 2025217. Aquabacter cavernae 1-5 are products obtained by iron filings oxidation in the process of denitrification. The present application provides a new insight for the repair of low carbon-nitrogen ratio water body.

The present application relates to a kind of secondary biochemical effluent ferrous iron synergistic mixed culture denitrification method, to the secondary biochemical effluent of urban sewage or industrial wastewater treatment plant, then enter the baffling denitrification reaction tank of iron shavings-pyrite composite filling and activated sludge floc, occur heterotrophic denitrification process, autotrophic denitrification process under the action of denitrifying bacteria;After treatment, effluent enters the center pipe of vertical flow sedimentation tank, is discharged after precipitation, and part of sludge is discharged as excess sludge.The present application utilizes the heterotrophic denitrification, Fe 0 And Fe 2+ Autotrophic denitrification and sulfur autotrophic denitrification maintain water acid-base balance, improve denitrifying bacteria abundance, promote sulfur, iron autotrophic denitrification bacteria and heterotrophic denitrification bacteria synergistic denitrification effect, so as to improve denitrification efficiency.The method of the present application is easy to operate and manage, and has low operating cost, which can effectively reduce sludge production.

The invention discloses a metal ion chelate for inhibiting urease and / or nitration, the general molecular formula of the structure is MDPA, M is a metal ion, and DPA is pyridine-2, 6-dicarboxylic acid; the invention also discloses a preparation method of the metal ion chelate for inhibiting urease and / or nitration. According to the invention, pyridine-2, 6-dicarboxylic acid is adopted as a ligand, and a polydentate coordination method is adopted for preparation, so that the compound is excellent in structural stability, simple in preparation process, outstanding in repeatability and operability, remarkable and efficient in urease inhibition performance, and capable of slowing down the degradation speed of urea and reducing the loss amount of nitrogen in soil; the compound fertilizer has the advantages that the nitrogen utilization rate is increased, favorable conditions are created for crop growth, the compound fertilizer shows an inhibition effect on nitrobacteria, the conversion process from ammonium nitrogen to nitrate nitrogen in soil can be effectively reduced, the nitrogen emission in the fertilizer release process is reduced, the compound fertilizer is environment-friendly, and the absorption efficiency of fertilizer nutrients can be improved.

The utility model provides a golden crucian carp culture device with water quality monitoring and adjusting functions, which comprises a culture box for golden crucian carp culture, a support frame arranged at the bottom of the culture box, a water quality adjusting component arranged on the support frame and used for water quality adjustment, and an adjusting box fixedly connected to the top of the support frame, water in the breeding box flows into the water draining plate through the water draining pipe and drips into the physical intercepting cavity through the rain holes, so that the oxygen content of the water is increased, fish feces are intercepted through filter cotton in the physical intercepting cavity, nitrobacteria are cultured through the bacteria room, harmful substances in the water are decomposed, and harm to the fish is reduced; water in the adjusting box is pumped out through the water pump and sprayed out through the water outlet holes, circulation of water flow is achieved, water is circularly purified, and the stability of water quality is guaranteed. And water is sprayed out from the water outlet holes and flows out from the water outlet, so that the water in the culture box flows, and fishes at the bottom of the culture box are cleaned.

The application relates to the field of environmental microorganism technology and discloses a salt-tolerant aerobic denitrification bacterium and a method for simultaneously realizing denitrification and carbonate precipitation and carbon fixation. Zobellella denitrificans A36, which is preserved in the Guangdong Microbial Culture Collection Center and has a preservation number of GDMCC No: 66789 and a preservation date of August 7, 2025. The Zobellella denitrificans A36 has complete denitrification key functional genes and carbonic anhydrase related gene clusters; can induce the formation of carbonate precipitation in a salt-containing heterotrophic denitrification system, fixes CO2 generated by the system into inorganic carbonate, and effectively reduces CO2 emission. Under preferred conditions, the removal rate of nitrate nitrogen can reach 93.8%, and stable carbonate carbon fixation effect is realized.

The present invention discloses a method and application of using polylactic acid plastic to cooperate with rhamnolipid to improve sewage treatment efficiency, and belongs to the field of water treatment and environmental protection technology. The present invention purifies and cultures the extracted microorganisms, and then conducts colonization experiments of microorganisms, density determination of suspended microorganisms, concentration determination of microorganisms on the surface of polylactic acid, and analysis of microbial community structure. According to the experimental results, the optimization effect of using polylactic acid plastic to cooperate with rhamnolipid to improve sewage treatment efficiency is evaluated. The method uses polylactic acid plastic pretreated with rhamnolipid as a filler for microorganisms. Through the synergistic effect of polylactic acid plastic and rhamnolipid, the colonization amount and activity of microorganisms on the surface of polylactic acid plastic are improved, the density of suspended microorganisms is increased, and the characteristic that polylactic acid particles are easily colonized by microorganisms after rhamnolipid is attached is brought into play, so as to adjust the proportion of microorganisms such as degrading complex organic matter and nitrifying bacteria, and optimize the application of sewage biochemical treatment process.

The invention discloses an intelligent fish tank system based on multi-water-quality-parameter fusion evaluation and full-automatic ecological regulation and control, and belongs to the field of intelligent aquarium breeding equipment. The system comprises a main control unit, a multi-class water quality sensor, an electromagnetic valve group, a pH adjusting and nitrifying bacteria adding module and a built-in multi-culture-variety exclusive parameter library. And the main control unit comprehensively evaluates the temperature, pH, TDS and turbidity through a fusion algorithm, and can pre-judge the fish disease risk and calculate the water quality health score. The system can automatically complete water change and pollution discharge, pH closed-loop regulation and control, strain supplementation and water temperature adjustment, realizes unattended operation in the whole process, greatly improves the water quality stability, reduces the fish disease probability, and is suitable for family breeding scenes.

The application discloses an ecological cultivation method of clownfish fry, which comprises the following steps: S1, constructing an ecological cultivation tank: a circulation system cultivation tank comprising a main tank and a bottom tank is arranged, the bottom tank comprises a lower water compartment, an algae tank compartment and an upper water compartment which are sequentially communicated, and the lower water compartment and the upper water compartment are communicated with the main tank to form a circulation; S2, cleaning and disinfecting the cultivation tank, and then injecting clean seawater into the cultivation tank; S3, water treatment: culturing nitrifying bacteria in the main tank to construct a perfect circulating water ecological system; S4, feeding fish and algae: feeding clownfish fry into the main tank and feeding large algae into the algae tank compartment; S5, daily management: feeding special feed for clownfish fry into the main tank at regular time, regularly illuminating the cultivation tank, and regularly detecting water quality and water replacement of the cultivation tank; and S6, harvesting: when the clownfish grows to 3-5 cm of a commercial specification, the cultivation is ended, and the clownfish and the large algae are harvested.

The invention discloses a pseudomonas strain, the pseudomonas strain is a heterotrophic nitrification and aerobic denitrification bacterium DHZK147, the pseudomonas strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO: 34640. The strain obtained through screening and separation has the efficient denitrification capacity and can achieve synchronous nitrification and denitrification. Compared with the traditional anaerobic denitrifying bacteria, the method has the advantages that the treatment time and cost of the process are saved in the biological denitrification process, and the effective removal of ammonia nitrogen is realized.

The application discloses a heterotrophic nitrifying bacterium D31, which is classified as Geobacillus proteiniphilus and has a strain preservation number of CGMCC NO.27788. The strain D31 can perform heterotrophic nitrification on a culture medium containing ammonia nitrogen, can grow on a simple carbon and nitrogen source culture medium and oxidize ammonia. The strain D31 can utilize simple nutrients to rapidly proliferate, is inoculated into compost after aerobic fermentation, can efficiently and rapidly oxidize ammonia nitrogen in livestock and poultry manure into nitrate nitrogen in a high-temperature period, reduces the volatilization of nitrogen elements in the form of ammonia gas in the compost, can not only reduce odor emission and protect the environment, but also can maintain nitrogen nutritional elements in the compost, improves compost quality, and has a wide application prospect. The application further provides application of the heterotrophic nitrifying bacterium D31 in livestock and poultry manure composting.

The application discloses a heterotrophic nitrification-aerobic denitrification bacterium and application thereof in sewage denitrification treatment, and belongs to the technical field of microorganisms. Enterobacter asburiae The heterotrophic nitrification-aerobic denitrification bacterium is Enterobacter asburiae WZCH (WZCH), which was preserved in the China Center for Type Culture Collection on June 16, 2025, and the preservation number is CCTCC NO: M 2025272. The strain has high reduction capacity, can convert ammonia nitrogen, nitrate nitrogen and the like in sewage into gaseous nitrogen, has no nitro nitrogen accumulation, is green and environment-friendly, and has high practical application value.

The invention relates to the technical field of microbial engineering and sewage treatment, and discloses a cold-resistant nitrobacterium strain, which is named as Nitrosomonas europaea SZG-AOB-002, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M20232716, is preserved in Wuhan University, Wuhan, China on December 29, 2023, and has the preservation number of CCTCC NO: M20232716. The invention aims to provide an autotrophic pure strain which is clear in source, stable in heredity and capable of realizing efficient ammoxidation in an environment of 8-15 DEG C, and a low-temperature high-density fermentation method adaptive to an industrial fermentation platform is established in a matched manner, so that engineering application of the autotrophic pure strain in low-temperature sewage treatment is realized.

The invention discloses a water curtain type net-shaped hydrophobic aeration membrane water body purification device, and relates to the technical field of wastewater treatment.The water curtain type net-shaped hydrophobic aeration membrane water body purification device comprises a water flow purification mechanism, the water flow purification mechanism comprises two fixing plates, each fixing plate is of a square plate-shaped structure, a rotating shaft is fixedly connected between the two fixing plates, and the rotating shaft is fixedly connected with the water curtain type net-shaped hydrophobic aeration membrane; a water flow purification mechanism is arranged, a hydrophobic membrane filter element is wrapped with a connecting frame through a hinge plate, sunlight can be isolated, nitrobacteria culture is achieved, water flows out naturally through a gap between a second water passing groove and a first water passing groove when flowing, circulation of the water flow is guaranteed, the water flow can evenly pass through the hydrophobic membrane filter element, and the nitrobacteria culture efficiency is improved. The sunlight can be isolated in a semi-closed manner while oxygen introduction is maintained, so that the problem that the nitrobacteria in the hydrophobic membrane filter element are killed by ultraviolet rays in the sunlight in a large quantity, and the water quality purification efficiency of the nitrobacteria is reduced is solved.

The invention relates to the technical field of water pollution control and treatment, in particular to a nitrifying bacteria consortium, a preparation method thereof and application of the nitrifying bacteria consortium in wastewater treatment. The nitrobacteria consortia comprises nitrosomonas sp. And nitrobacter winogradsky, and a compact and spherical cenobium structure is formed; the nitrification rate of the continuous passage of the nitrifying bacterium consortium is stabilized between 200-300mg NH4 < + >-N / L / h, so that the long-term stability of the nitrifying bacterium community is ensured; meanwhile, ammonia nitrogen can still be efficiently degraded under the extreme environmental conditions of high salt, high alkalinity, high aniline content and the like.

The invention discloses nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01, the preservation number of the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01 is GDMCC No: 65729, the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01 has been preserved in Guangdong Microbial Culture Collection Center on March 7, 2025, is called GDMCC for short, has the preservation address of Nitrobacter sp., and is classified and named as Nitrobacter sp., and the preservation name of the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp. TKz-YC01 is Nitrobacter sp. The nitrobacter can effectively degrade nitrite, cyanate and / or ammonium salt in water, and the nitrobacter can still effectively remove nitrogen under the impact of 30 mM ammonium salt (ammonium chloride) and 50 mM nitrite (sodium nitrite).

The present invention discloses a multifunctional combined module for a floating wetland of a stagnant water body, and relates to the technical field of wetland ecological restoration. The present invention provides an inner basket and an outer basket, and loads a planting matrix, a denitrification enhancer slow-release tank or a dephosphorization enhancer slow-release tank in the inner basket, so that the entire combined module is divided into a plurality of units with different functions. Through the provision of a card plate, on the one hand, the root system of the plant is pruned by the sharp edge of the card plate to maintain a certain length of the plant root system; on the other hand, the opening of the outer basket loaded with the planting matrix module unit is periodically opened and closed, so that the denitrification enhancer and the dephosphorization enhancer are maintained at a high concentration in the outer basket, thereby preventing the enhancer from being directly diluted by the stagnant water body, and purifying the stagnant water body in combination with beneficial bacteria such as nitrifying bacteria, denitrifying bacteria, sulfate-reducing bacteria, etc. produced by plants planted in the planting matrix.