47 results about "Bacterium nitrobacter" patented technology

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses a filler reinforced ecological floating bed for purifying aquaculture tail water and application thereof, and belongs to the technical field of water treatment. The filler reinforced ecological floating bed comprises canna cultivated in a floating plate and straw or straw biochar suspended below the floating plate; the straw biochar is obtained by taking straw as a raw material and pyrolyzing the straw at 500 DEG C or 700 DEG C. The ecological floating bed capable of purifying the aquaculture tail water is prepared by utilizing the emergent aquatic plant canna which has high purification efficiency on nitrogen and phosphorus and the straw or straw biochar which has a large surface area and is beneficial to the growth of denitrifying bacteria, and the ecological floating bed can effectively reduce the content of nitrogen and phosphorus in the aquaculture tail water, accelerate the denitrification and dephosphorization rate and improve the quality of the aquaculture tail water. The tail water purification method can be used for reducing nitrate accumulation, realizing tail water purification, providing scientific basis and technical support for tail water treatment work in aquaculture, and being beneficial to promoting green and sustainable development of aquaculture.

The invention discloses a bottom mud ecological restoration material as well as a preparation method and application thereof. The material is composed of a degradable carrier, a treatment flora and a multifunctional filler. The carrier is porous particles die-cast by corn straws and the like; the treatment flora comprises photosynthetic bacteria, bacillus and nitrifying bacteria; the filler is prepared from non-nitrate type hydrotalcite-like compounds, calcium nitrate, nitrate type hydrotalcite-like compounds and a flocculating agent according to a specific mass ratio. The preparation method comprises the following steps: carrying out pressure casting on the carrier, adsorbing the flora, and rolling the filler to form balls. The restoration method comprises the steps that a river channel is cut off in the dry season to form a closed area, materials are put according to the area ratio of 10: 1 for preliminary restoration, and after standing is conducted for 1-5 days, the materials are put along with water flow according to the area ratio of 2: 1 for deep restoration. Through the physical, chemical and biological synergistic effect, the sediment redox environment can be effectively improved, pollutant release and degradation are promoted, operation is easy and convenient, and the risk of secondary pollution is low.

The invention relates to the technical field of microbial fermentation, and particularly discloses a high-density expanding culture method of autotrophic nitrifying bacteria. The method comprises the following steps: screening low-temperature aquatic plants, and carrying out stepped pressurization domestication to obtain low-temperature-resistant and high-salt-resistant autotrophic nitrobacteria strains; a three-stage stepped expanding culture process is adopted, and a carrier filler with a high specific surface area is added in second-stage fermentation and third-stage fermentation; in the fermentation process, continuous collaborative fed-batch feeding, pH linkage regulation and control and dissolved oxygen precise linkage control of an inorganic carbon and nitrogen source are implemented. Through stress-resistant strain screening, carrier adding and precise process control, the density of the thalli in the fermentation liquor can be increased, the stress resistance of the thalli is improved, and the method is suitable for a more complex water body environment.

The utility model discloses a blowdown pipeline of a saturation tower. The blowdown pipeline comprises a main pipeline, and a front-end filtering heat exchanger, a plate heat exchanger and a sewage treatment tank which are sequentially arranged from upstream to downstream, the front-end filtering heat exchanger comprises a shell and a plurality of heat absorption layers, each heat absorption layer comprises a plurality of inverted-V-shaped hollow plate bodies which are evenly distributed in the horizontal direction, and the hollow plate bodies of every two adjacent heat absorption layers are distributed in a staggered mode. The shell is also provided with a sewage inlet, a sewage outlet, an impurity outlet, a water replenishing port, a water dividing tank and a water collecting tank, the impurity outlet is provided with a discharge pipe, and the downstream end of the discharge pipe is connected with the sewage treatment tank; two ends of the hollow plate body are respectively communicated with the water distribution tank and the water collection tank; the temperature of sewage entering the plate heat exchanger is reduced in advance through the front-end filtering heat exchanger, so that abnormal temperature rise of the subsequent sewage treatment tank is avoided, and nitrobacteria culture of the sewage treatment tank is facilitated; in addition, the front-end filtering heat exchanger also realizes coarse filtration and precipitation of sewage, so that the blockage problem of the plate heat exchanger is effectively reduced.

The invention relates to a water regulating microbial preparation for breeding largemouth bass seedlings in a land-based round pond and application, and belongs to the technical field of aquaculture. The preparation aims to solve the problem of combined pollution caused by synchronous exceeding of ammonia nitrogen, nitrite and hydrogen sulfide in a water body due to accumulation of residual feed and excrement in a land-based round pool high-density breeding environment. According to the scheme, the microbial agent is prepared by physically mixing bacillus standard bacterial powder, photosynthetic bacteria standard bacterial powder, compound nitrifying bacteria standard bacterial powder and thiobacillus standard bacterial powder according to specific parts by weight, wherein the bacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis, and the thiobacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis. The composite nitrifying bacteria standard bacterial powder is formed by mixing nitrite bacteria and nitrifying bacteria, and the thiobacillus thiooxidans standard bacterial powder is formed by mixing thiobacillus thiooxidans and thiobacillus thioparus. The preparation is used for synchronously degrading ammonia nitrogen, nitrite and hydrogen sulfide in aquaculture water, so that normal development of gonads of largemouth bass parents is guaranteed, and the hatching rate of fertilized eggs and the survival rate of early-stage seedlings are increased.

The application discloses a closed fishery circulating aquaculture system, which comprises an aquaculture device, a water circulation pipeline, a biochemical device, an oxygen supply device and a gas supply device. The aquaculture device comprises an aquaculture tank and a feeding assembly. The aquaculture tank is provided with an aquaculture cavity. The aquaculture tank is provided with a first water inlet pipe and a first water outlet pipe. The feeding assembly is arranged on the aquaculture tank. The water circulation pipeline is arranged between the first water inlet pipe and the first water outlet pipe. The biochemical device comprises a biochemical tank. The biochemical tank is provided with a biochemical cavity. The biochemical cavity is communicated with the water circulation pipeline. The biochemical cavity is used for accommodating nitrifying bacteria. The oxygen supply device is connected with the aquaculture tank and the biochemical tank. The gas supply device is provided with a gas conveying pipe between the aquaculture tank. The closed fishery circulating aquaculture system can not only meet the pressure requirement of aquatic products growing in different environmental pressures, but also make the water dissolving oxygen efficiency in the aquaculture cavity higher and be more beneficial to the growth of the aquatic products. In addition, the biochemical device is more beneficial to the growth of the photophobic and aerobic nitrifying bacteria.

The application discloses a preparation method of a porous sulfur mineral autotrophic denitrification material and application of the material in black and odorous water purification. The porous sulfur mineral functional material is prepared by using coral, iron-manganese oxide minerals and sulfur minerals, so that waste is reused, resources are saved, and the preparation method is simple; on the other hand, the porous sulfur mineral functional material has a large specific surface area and high activity. The high-activity porous sulfur mineral functional material can be used as an electron donor of sulfur autotrophic denitrification bacteria and denitrifying sulfur bacteria in a black and odorous water body with a low carbon-nitrogen ratio. Meanwhile, the coral, iron-manganese oxide minerals, nano zero-valent calcium and nano zero-valent iron-manganese formed by hydrogen reduction roasting, and the hydrated silicate formed by the sulfur mineral and cement improve the alkalinity in the reaction system, can neutralize the sulfur autotrophic denitrification acid production process, and realize deep purification of the black and odorous water body.

A strain of antibiotic-resistant marine heterotrophic ammonia assimilation-aerobic denitrifying bacteria and its application, belongs to the field of water treatment of aquaculture. The seawater antibiotic-resistant high-efficiency nitrogen removal Marinobacter nitratireducens LAN01 can use polyhydroxyalkanoate as the sole carbon source, and can realize heterotrophic ammonia assimilation and aerobic denitrification function under the stress of different concentrations of sulfamethoxazole antibiotics, and simultaneously transform phosphate. The strain has multiple resistances such as high salinity, high alkalinity and antibiotic, and can be used for seawater aquaculture effluent treatment, seawater recirculating aquaculture water treatment, high-salinity wastewater treatment and saline-alkali soil bioremediation, etc. To realize the simultaneous recovery of nitrogen, phosphorus and other nutrients in the seawater aquaculture environment.

The present application belongs to the technical field of water pollution treatment, and relates to microbial treatment, in particular to an aged iron filings promoted aerobic iron-reducing fungi for denitrification and application thereof. Aspergillus The aerobic iron-reducing fungi is classified and named as sp. R4, and is preserved in China Center for Type Culture Collection on February 13, 2025, with a preservation number of CCTCC NO: M 2025217. Aquabacter cavernae 1-5 are products obtained by iron filings oxidation in the process of denitrification. The present application provides a new insight for the repair of low carbon-nitrogen ratio water body.

The present application relates to a kind of secondary biochemical effluent ferrous iron synergistic mixed culture denitrification method, to the secondary biochemical effluent of urban sewage or industrial wastewater treatment plant, then enter the baffling denitrification reaction tank of iron shavings-pyrite composite filling and activated sludge floc, occur heterotrophic denitrification process, autotrophic denitrification process under the action of denitrifying bacteria;After treatment, effluent enters the center pipe of vertical flow sedimentation tank, is discharged after precipitation, and part of sludge is discharged as excess sludge.The present application utilizes the heterotrophic denitrification, Fe 0 And Fe 2+ Autotrophic denitrification and sulfur autotrophic denitrification maintain water acid-base balance, improve denitrifying bacteria abundance, promote sulfur, iron autotrophic denitrification bacteria and heterotrophic denitrification bacteria synergistic denitrification effect, so as to improve denitrification efficiency.The method of the present application is easy to operate and manage, and has low operating cost, which can effectively reduce sludge production.

The invention discloses a metal ion chelate for inhibiting urease and / or nitration, the general molecular formula of the structure is MDPA, M is a metal ion, and DPA is pyridine-2, 6-dicarboxylic acid; the invention also discloses a preparation method of the metal ion chelate for inhibiting urease and / or nitration. According to the invention, pyridine-2, 6-dicarboxylic acid is adopted as a ligand, and a polydentate coordination method is adopted for preparation, so that the compound is excellent in structural stability, simple in preparation process, outstanding in repeatability and operability, remarkable and efficient in urease inhibition performance, and capable of slowing down the degradation speed of urea and reducing the loss amount of nitrogen in soil; the compound fertilizer has the advantages that the nitrogen utilization rate is increased, favorable conditions are created for crop growth, the compound fertilizer shows an inhibition effect on nitrobacteria, the conversion process from ammonium nitrogen to nitrate nitrogen in soil can be effectively reduced, the nitrogen emission in the fertilizer release process is reduced, the compound fertilizer is environment-friendly, and the absorption efficiency of fertilizer nutrients can be improved.

The application relates to the field of environmental microorganism technology and discloses a salt-tolerant aerobic denitrification bacterium and a method for simultaneously realizing denitrification and carbonate precipitation and carbon fixation. Zobellella denitrificans A36, which is preserved in the Guangdong Microbial Culture Collection Center and has a preservation number of GDMCC No: 66789 and a preservation date of August 7, 2025. The Zobellella denitrificans A36 has complete denitrification key functional genes and carbonic anhydrase related gene clusters; can induce the formation of carbonate precipitation in a salt-containing heterotrophic denitrification system, fixes CO2 generated by the system into inorganic carbonate, and effectively reduces CO2 emission. Under preferred conditions, the removal rate of nitrate nitrogen can reach 93.8%, and stable carbonate carbon fixation effect is realized.

The invention discloses an intelligent fish tank system based on multi-water-quality-parameter fusion evaluation and full-automatic ecological regulation and control, and belongs to the field of intelligent aquarium breeding equipment. The system comprises a main control unit, a multi-class water quality sensor, an electromagnetic valve group, a pH adjusting and nitrifying bacteria adding module and a built-in multi-culture-variety exclusive parameter library. And the main control unit comprehensively evaluates the temperature, pH, TDS and turbidity through a fusion algorithm, and can pre-judge the fish disease risk and calculate the water quality health score. The system can automatically complete water change and pollution discharge, pH closed-loop regulation and control, strain supplementation and water temperature adjustment, realizes unattended operation in the whole process, greatly improves the water quality stability, reduces the fish disease probability, and is suitable for family breeding scenes.

The application discloses an ecological cultivation method of clownfish fry, which comprises the following steps: S1, constructing an ecological cultivation tank: a circulation system cultivation tank comprising a main tank and a bottom tank is arranged, the bottom tank comprises a lower water compartment, an algae tank compartment and an upper water compartment which are sequentially communicated, and the lower water compartment and the upper water compartment are communicated with the main tank to form a circulation; S2, cleaning and disinfecting the cultivation tank, and then injecting clean seawater into the cultivation tank; S3, water treatment: culturing nitrifying bacteria in the main tank to construct a perfect circulating water ecological system; S4, feeding fish and algae: feeding clownfish fry into the main tank and feeding large algae into the algae tank compartment; S5, daily management: feeding special feed for clownfish fry into the main tank at regular time, regularly illuminating the cultivation tank, and regularly detecting water quality and water replacement of the cultivation tank; and S6, harvesting: when the clownfish grows to 3-5 cm of a commercial specification, the cultivation is ended, and the clownfish and the large algae are harvested.

The application discloses a heterotrophic nitrifying bacterium D31, which is classified as Geobacillus proteiniphilus and has a strain preservation number of CGMCC NO.27788. The strain D31 can perform heterotrophic nitrification on a culture medium containing ammonia nitrogen, can grow on a simple carbon and nitrogen source culture medium and oxidize ammonia. The strain D31 can utilize simple nutrients to rapidly proliferate, is inoculated into compost after aerobic fermentation, can efficiently and rapidly oxidize ammonia nitrogen in livestock and poultry manure into nitrate nitrogen in a high-temperature period, reduces the volatilization of nitrogen elements in the form of ammonia gas in the compost, can not only reduce odor emission and protect the environment, but also can maintain nitrogen nutritional elements in the compost, improves compost quality, and has a wide application prospect. The application further provides application of the heterotrophic nitrifying bacterium D31 in livestock and poultry manure composting.

The application discloses a heterotrophic nitrification-aerobic denitrification bacterium and application thereof in sewage denitrification treatment, and belongs to the technical field of microorganisms. Enterobacter asburiae The heterotrophic nitrification-aerobic denitrification bacterium is Enterobacter asburiae WZCH (WZCH), which was preserved in the China Center for Type Culture Collection on June 16, 2025, and the preservation number is CCTCC NO: M 2025272. The strain has high reduction capacity, can convert ammonia nitrogen, nitrate nitrogen and the like in sewage into gaseous nitrogen, has no nitro nitrogen accumulation, is green and environment-friendly, and has high practical application value.

The invention relates to the technical field of microbial engineering and sewage treatment, and discloses a cold-resistant nitrobacterium strain, which is named as Nitrosomonas europaea SZG-AOB-002, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M20232716, is preserved in Wuhan University, Wuhan, China on December 29, 2023, and has the preservation number of CCTCC NO: M20232716. The invention aims to provide an autotrophic pure strain which is clear in source, stable in heredity and capable of realizing efficient ammoxidation in an environment of 8-15 DEG C, and a low-temperature high-density fermentation method adaptive to an industrial fermentation platform is established in a matched manner, so that engineering application of the autotrophic pure strain in low-temperature sewage treatment is realized.

The invention relates to the technical field of water pollution control and treatment, in particular to a nitrifying bacteria consortium, a preparation method thereof and application of the nitrifying bacteria consortium in wastewater treatment. The nitrobacteria consortia comprises nitrosomonas sp. And nitrobacter winogradsky, and a compact and spherical cenobium structure is formed; the nitrification rate of the continuous passage of the nitrifying bacterium consortium is stabilized between 200-300mg NH4 < + >-N / L / h, so that the long-term stability of the nitrifying bacterium community is ensured; meanwhile, ammonia nitrogen can still be efficiently degraded under the extreme environmental conditions of high salt, high alkalinity, high aniline content and the like.

The invention discloses nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01, the preservation number of the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01 is GDMCC No: 65729, the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01 has been preserved in Guangdong Microbial Culture Collection Center on March 7, 2025, is called GDMCC for short, has the preservation address of Nitrobacter sp., and is classified and named as Nitrobacter sp., and the preservation name of the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp. TKz-YC01 is Nitrobacter sp. The nitrobacter can effectively degrade nitrite, cyanate and / or ammonium salt in water, and the nitrobacter can still effectively remove nitrogen under the impact of 30 mM ammonium salt (ammonium chloride) and 50 mM nitrite (sodium nitrite).

The invention relates to a preparation method of a nitrifying bacterium agent block, which comprises the following steps: mixing and grinding a mineral carrier to a specified particle size, and respectively preparing a PVA (Polyvinyl Alcohol) solution and a composite adhesive; mixing and kneading the pretreated mineral carrier with a PVA solution, then adding a composite adhesive, kneading, and finally adding the bacterial suspension for embedding to prepare a dough-shaped material; filling a double-layer nested mold with the dough-shaped material in batches, and respectively applying different pressures to form a gradient structure fungicide block which is dense outside and sparse inside; soaking the formed microbial agent block in a calcium chloride solution for cross-linking reaction; and sequentially carrying out normal-temperature pre-curing and low-temperature activation on the cured microbial agent block to obtain a finished product. The problems that in the prior art, the survival rate of flora is low and the slow release period is short are solved by combining a composite mineral carrier with a specific formula and a multi-component organic adhesive with a unique gradient compaction mold forming and calcium ion cross-linking curing process.

The application discloses a treatment preparation for in-situ repair of river water bodies and a preparation method and application thereof, and the treatment preparation comprises the following components in parts by weight: 5-10 parts of composite microorganisms, 10-20 parts of inulin, 60-80 parts of resistant dextrin, 20-40 parts of oligoisomaltooligosaccharide, 10-20 parts of activated carbon, 10-20 parts of attapulgite and 5-10 parts of calcium peroxide; each part of the composite microorganisms comprises 10-20 parts of Rhodopseudomonas palustris, 5-10 parts of Trichoderma harzianum, 4-8 parts of Bacillus subtilis and 4-8 parts of denitrifying bacteria; the treatment preparation can remove particulate matters, organic matters, ammonia nitrogen and phosphorus in the river water bodies and can realize rapid repair of the river water bodies through selection of raw materials and limitation of core-shell structures.

Additive mixture for fermentation liquids, containing at least the following components: (A) at least one type of activated carbon containing at least one absorbed or adsorbed or absorbed and adsorbed inorganic nitrate, (B) (b1) NADH and / or NADPH nitrate reductase obtained from plants or yeasts or (b2) at least one type of denitrifying bacteria in free form or (b3) at least one type of denitrifying bacteria carried on and / or in at least one type of activated carbon or (b4) at least one type of denitrifying bacteria in free form and supported on and / or in at least one type of activated carbon or (b5) NADH and / or NADPH nitrate reductase obtained from plants or yeasts and at least one type of denitrifying bacteria in free form or (b6) NADH and / or NADPH nitrate reductase derived from plants or yeasts and at least one type of denitrifying bacteria carried on and / or in at least one type of activated carbon or (b7) NADH and / or NADPH nitrate reductase derived from plants or yeasts, at least one type of denitrifying bacteria in free form and at least one type of denitrifying bacteria carried on and / or in at least one type of activated carbon each in a total quantity that is insufficient to replace the nitrate present in (A) according to equation (I) NO3 - +2 H + + 2 e - = NO2 - + H2O (I) to reduce completely to nitrite, and (C) the bacterium Candidatus Brocadia Anammoxidans in free form or supported on and / or in at least one type of activated carbon or in free form and supported on and / or in at least one type of activated carbon, each in a total quantity sufficient to remove the nitrite resulting according to equation (I) and the ammonia present in the fermentation liquid according to equation (II): NH4 + + NO2 - = N2 + H2O (II), to proportionate to nitrogen.

The present application relates to sewage biological treatment technical field, specifically relates to a kind of light control extracellular polymer solution and its preparation method and application.The present application provides a kind of preparation method of light control extracellular polymer solution, comprising the following steps: (1) using first nitrate wastewater acclimation activated sludge, obtain acclimation activated sludge;(2) using second nitrate wastewater activation the acclimation activated sludge, obtain activated activated sludge;(3) extract the bacteria in the activated activated sludge, then carry out light control metabolic reaction under blue light irradiation in culture solution, after reaction, extract active substance, i.e. the light control extracellular polymer solution is obtained.The present application is irradiated by blue light in light control metabolic reaction to open downstream path or signal transmission, and then open rare metabolic path to generate the light control extracellular polymer solution with biological activity, and the light control extracellular polymer solution can have catalytic effect to improve the denitrification efficiency of denitrifying bacteria.

1. The name of the design product: bacteria brick (bacteria cube mineral brick). 2. The use of the design product: for the culture of aquarium microbial nitrifying bacteria, and water filtration and purification, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention provides a novel bacteriophage of soil denitrifying bacteria and application thereof, and belongs to the technical field of microorganisms. The novel bacteriophage is a bacillus cereus bacteriophage, is named as PHBA67-J and belongs to a tailed bacteriophage, the genome of the novel bacteriophage is double-stranded linear DNA, the full length is 42 and 152 bp, and the GC content is 34.86%. An original enrichment solution of the bacteriophage PHBA67-J is diluted with an SM buffer solution to prepare a bacteriophage suspension with the activity of 1.0 * 10 < 13 > PFU.mL <-1 >, and after 10% (v / w) of active PHBA67-J bacteriophage suspension is inoculated and cultured in darkness for 14 days, the denitrification potential of soil can be remarkably reduced, the content of ammonium nitrogen and nitrate nitrogen in the soil can be remarkably increased, and the nitrogen utilization rate of farmland soil can be improved.

The invention discloses an air optimization device and optimization system based on an unmanned ecosystem, the air optimization device comprises a shell and a top plate arranged above the shell, the shell is internally provided with vertically distributed partition plates, and a nitrifying bacteria room is used for neutralizing nitrogen elements in water; and the neutralized water is pumped into the water tank through the suction pipe and the water pump connected with the suction pipe. According to the air optimization equipment based on the unmanned ecosystem and the optimization system, indoor air is disinfected in an aerosol sterilization mode through a winding and transparent pipeline, the air quality and the purification effect can be improved, the safety of a water body environment in a water-based area can be guaranteed, internal structures of the water-based area and a land-based area are redesigned, and the air quality is improved. While the air purification effect is guaranteed, excrement of aquatic animals is comprehensively utilized and treated, the cleanliness degree of the water-based environment and the growth requirements of plants in the land-based environment are guaranteed, and more energy conservation and environmental protection are achieved.

The invention belongs to the technical field of fertilizers, and particularly relates to a preparation method of an organic compound and application of the organic compound in soil. The organic compound is prepared from the following raw materials: humic acid, maltodextrin, a microbial agent, polyacrylamide, oat bran, cow dung and brown algae. The microbial agent comprises nitrifying bacteria, rhizobium radiobium, bacillus licheniformis and bacillus megatherium. According to the invention, the raw materials are compounded for use, and the raw materials have a synergistic effect, so that the efficient organic compound capable of improving the soil fertility is provided. The raw materials of the organic compound are easy to obtain, the rice ear number is remarkably increased, the rice seed number per ear is increased, the rice dry grain weight is increased, the rice yield is effectively increased, the potato yield is effectively increased, and good economic benefits and social benefits are achieved.

According to the preparation method of the light filter material for deeply removing the total nitrogen and the total phosphorus, the foaming porous filter material is prepared by taking the steel slag, the zeolite, the lime and the like as main components, and the sludge is cultivated in the starting period, so that the tolerance of microorganisms to sulfide toxicity is improved; sulfur and cultured strains of autotrophic thiobacillus denitrificans and sulfate reducing bacteria are solidified on the porous filter material through a biological embedding technical means, so that autotrophic denitrification of elemental sulfur and reduction of sulfate radicals are realized. The porous structure of the light filter material is beneficial to transmission of substances such as nitrogen and phosphorus, the surface of the filter material can provide conditions for attachment and growth of microorganisms such as phosphorus-accumulating bacteria, nitrifying bacteria, thiobacillus denitrificans and sulfate reducing bacteria, the high porosity and large specific surface area of the filter material can bring a large microorganism attachment amount, and the huge specific surface area is beneficial to growth of a biological membrane, so that the biological membrane can be effectively degraded. The filter material is subjected to combined treatment through a solid physicochemical adsorption method and a microbiological method, the pollutant removal capacity is high, and the effect is remarkable.

The application discloses a salt-tolerant heterotrophic nitrification-aerobic denitrification bacterium. Halomonas elongata 5300, and the preservation number is CGMCC No.25768. The strain has a wide salt concentration tolerance range, can grow under a salt concentration of 0% to 26%, and can efficiently remove NO2 ‑ -N and NH4 + -N in water under a salt concentration of 4% to 16%. The strain 5300 can effectively solve the problem of activity inhibition of functional microorganisms in nitrogen-containing polluted water under high salt in a previous biological denitrification process, and is suitable for denitrification treatment of nitrogen-containing polluted water under high salt and aerobic conditions. ‑ -N removal efficiency reaches 100.00%, and NH4 + -N is more than 78.51%. The strain 5300 can effectively solve the problem of activity inhibition of functional microorganisms in nitrogen-containing polluted water under high salt in a previous biological denitrification process, and is suitable for denitrification treatment of nitrogen-containing polluted water under high salt and aerobic conditions.