165 results about "Paracoccus" patented technology

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.

The invention relates to aerobic denitrifying paracoccus denitrificans and application thereof. A bacterial strain provided in the invention is paracoccus denitrificans DN-3CGMCC No. 3658; the Paracoccus denitrificans can carry out aerobic denitrification by using nitrate nitrogen under both aerobic and oxygen-limited conditions and can also carry out heterotrophic nitrification-aerobic denitrification by using ammonia-N, and a total nitrogen removal rate on above mentioned occasions is greater than 90%. The paracoccus denitrificans provided in the invention has stable hereditary features, isapplicable to treatment of a variety of nitrogen-containing waste water and produces a good nitrogen removal effect.

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.

The invention discloses a pyridine-degrading bacterium as well as a composite bacterial agent, a preparation method and uses thereof. The newly-separated Rhodococcus pyridinovora KT-J002 CGMCC No.2789 and Cellulomonas sp KT-J007 CGMCC No.2788 not only can effectually degrade the high concentration pyridine, but also has tolerance or degradation ability for toxic substances such as benzene, phenol, dimethylbenzene, quinoline, cyanide and the like; the composite bacterial agent composed of Rhodococcus pyridinovora KT-J002 CGMCC No.2789, Cellulomonas sp KT-J007 CGMCC No.2788, Paracoccus d enitrificans W12 CGMCC No.1673, Micrococcus luteus ATCC 49442 and Arthrobacter crystallopoietes ATCC 15481 is used for environmental improvement, can effectually degrade the pyridine and is particularly suitable for the biological treatment of coking waste water.

The invention relates to a biological agent for processing industrial sewage, a preparation method and application. The biological agent is prepared by the following raw materials in percentage by weight; 25% of rhodococcus, 20% of bacillus subtilis, 15% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 10% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the trains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by conventional activated sludge process.

The invention belongs to the technical field of environmental organisms, and relates to paracoccus denitrifican with heterotrophic denitrification, autotrophic denitrification and iron reduction functions and a culturing method and an application thereof. The bacterium is paracoccus denitrifican ZGL1 with the collection number CCTCC M2012158, and can be used for completing heterotrophic denitrification and autotrophic denitrification processes (including sulfur autotrophic denitrification and iron autotrophic denitrification) and effectively reducing ferric iron. During waste gas treatment, reduction of Fe(II)EDTA-NO and regeneration of Fe(II)EDTA are realized simultaneously for the same strain in a BioDeNOX denitration process, the aim of continuously denitrating is fulfilled, and low energy consumption, small investment and low running cost are realized. During waste water treatment, the strain can be used for removing nitrate and nitrite from waste water in a heterotrophic denitrification process, can be applied to waste water denitrification through sulfur autotrophic denitrification and iron autotrophic denitrification processes, is low in cost, and has a simple process.

The invention discloses a denitrogenation paracoccus strain and the cultivating method and the application. The strain is Paracoccus denitrificans W12 CGMCC No.1673. It has the strong degradation ability to organic pollutant, and could be used to take the biology treatment to the polluted water containing benzene, xylene, quinoline and cyanide and to the polluted soil. Especially, it has high degradation activity to benzene.

The invention relates to a microorganism bacterium agent for treating industrial wastewater pollution. The microorganism bacterium agent is prepared from pseudomonas putida, candida utilis, bacillus amyloliquefaciens, clostridium papyrosolvens, nitrosomonas europaea, paracoccus denitrificans and auxiliary materials. The microorganism bacterium agent has the advantages that various bacteria in mutualistic symbiosis are compatible reasonably without antagonism and are effective in degradation of phenyl amines degradation-resistant compounds when being thrown into a wastewater treatment system, thereby being suitable for industrial wastewater treatment and being capable of increasing treatment water quantity, improving treatment water quality, reducing operational cost and promoting up-to-standard discharge.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention relates to a cultural method for paracoccus denitrificans and application of the same to purifying aquaculture water, and belongs to the technical field of aquaculture. Various low-value materials such as molasses and waste liquid of monosodium glutamate and production waste which are harmless for aquaculture organisms and the paracoccus denitrificans are used as raw materials, the raw materials are reasonably compounded into liquid culture media, paracoccus denitrifican liquid with high activity and high concentration is produced, the liquid is directly fed into the aquaculture water, an ecological environment of the aquaculture water is repaired, and an excellent water environment beneficial to survival and growth is created for the aquaculture organisms. The culture method and the application have the advantages that the paracoccus denitrificans become effective fast after being used, an inorganic nitrogen removal form and the removal speed can be selected according to an oxygen dissolving condition of the water, contents of harmful substances such as ammonia nitrogen, nitrite and nitrate in the water are remarkably reduced, the quality of the water environment and a survival condition for the aquaculture organisms are improved, the yield of the aquaculture organisms is increased, and the quality of the aquaculture organisms is improved.

The invention belongs to the field of wastewater treatment and discloses a treatment process applicable to industrial wastewater. The treatment process applicable to the industrial wastewater comprises the following steps: 1) preparing complex microbial inoculant; 2) precipitating wastewater; 3) purifying the wastewater; 4) preparing bacterial manure. The prepared complex microbial inoculant comprises 5-6 parts of micrococcus luteus, 4-5 parts of bacillus mucilaginosus, 3-4 parts of paracoccus denitrificans, 2-3 parts of rhodococcus, 2-3 parts of acidithiobacillus ferrooxidans, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus cereus. The invention also discloses bacterial manure prepared by utilizing the treatment process applicable to the industrial wastewater. The treatment process applicable to the industrial wastewater is simple and feasible, the industrial wastewater can be effectively treated, and the bacterial manure is prepared by utilizing the treatment process applicable to the industrial wastewater.

The invention discloses an industrial sewage treatment agent, comprising polyacrylamide, silicon dioxide, peroxysulphate, sodium carbonate and compound microorganisms, wherein the compound microorganisms comprise bacillus licheniformis, pseudomonas aeruginosa, bacillus cereus, candida lipolytica, nitrifying bacteria and paracoccus denitrificans. By adopting the industrial sewage treatment agent disclosed by the invention, the removal rate of sewage suspension solids can be up to 99.8%, the biochemical oxygen demand (BOD) can be up to 98.7%, the chemical oxygen demand (COD) removal rate can be up to 98%, the industrial sewage treatment agent is less in sludge amount, free of secondary pollution, stable in treatment effect, environment-friendly, and non-toxic.

The invention discloses a Paracoccus aminovorans LH-N40 CGMCC No.6971. The invention also discloses a heterotrophic nitrification-aerobic denitrification microbial inoculum which contains the Paracoccus aminovorans LH-N40. The invention also discloses a preparation method of the microbial inoculum. The microbial inoculum disclosed by the invention can solve the problem of difficulty in removing total nitrogen in the traditional reactor, can effectively remove ammonia nitrogen and total nitrogen in the water body in one reactor, has tolerance or degradation capability for phenols, amines, heterocycles, cyanides, polycyclic aromatic hydrocarbons and other toxic substances in wastewater, is especially applicable to nitrogenous chemical wastewater, has the advantages of simple treatment process, stable effect and environmental toxicant impact resistance, and has very wide application prospects in various actual nitrogenous chemical wastewater treatment processes.

The invention relates to the technical field of biology and particularly relates to paracoccus denitrificans and an application thereof in treatment of livestock farm wastewater. The paracoccus denitrificans TDFQ-1 is collected at the China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO:M2017238. The bacterial strain has the advantages of being high in growth speed, high in environmental tolerance and good in growth in an organic culture medium. The paracoccus denitrificans has a heterotrophic nitrification capability and an aerobic denitrification capability, and is capable of reducing harmful substances, such as ammonia nitrogen and nitrate in water, especially removing the ammonia nitrogen in the livestock farm wastewater with relatively high organic matter content and improving the breeding environment. The bacterial strain is capable of efficiently degrading fluoroquinolone antibiotics when treating the wastewater, reducing the pollution of the antibiotics to soil and water sources and protecting the human health.

The invention relates to the technical field of microbes, and especially discloses a composite microbial preparation used for river treatment. The composite microbial preparation used for river treatment comprises a compound microorganism, and the compound microorganism is composed of the following raw materials in percent by weight: 1-2% of nitrosococcus, 5-8% of thiobacillus thiooxidans, 8-15% of paracoccus denitrificans, 20-30% of bacillus spp, 8-18% of rhodospira, 6-15% of lactobacillus spp, 10-15% of saccharomyces cerevisiae, and 20-30% of bacillus subtilis. After being poured into polluted water, the composite microbial preparation used for river treatment is capable of obviously improving the water body blackening smelliness problem, reducing water-quality ammonium nitrogen and total phosphorus content, decomposing hydrogen sulfide and other harmful substances and improving water body environment. The composite microbial preparation used for river treatment is capable of improving the absorption utilization efficiency of water plants on nutrition elements and obviously improving the water body transparency, and possesses the advantages of stabile microbial preparation, strong purification capability, simple preparation, low cost and applicability to river treatment.

Belonging to the field of microbial environmental protection, the invention provides a microbial agent for treatment of sewage and sludge. The microbial agent mainly includes Rhodospirillum rubrum, rhodopseudomonas palustris, bacillus subtilis, Streptomyces microflavus, streptococcus thermophilus, candida utilis, Streptomyces viridis, pseudomonas aeruginosa, virgibacillus halodenitrificans, and Paracoccus denitrificans 10 bacteria. Each bacterium is subjected to conventional slant culture, shaking culture and seed tank cultivation respectively, the bacteria in the seed tank are mixed and then put into sewage directly, symbiosis of flora is utilized to reach the purpose of treating sewage and reducing sludge generation. The microbial agent provided by the invention can be applied to large-scale production and direct adding, is low in cost, and can adapt to changeful sewage components. And the flora has good impact resistance to sewage.

The invention belongs to the technical field of biology and environment protection, and aims at providing paracoccus denitrificans and a method for disposing single-cell protein through high ammonia-nitrogen wastewater. The adopted technical scheme is as follows: the invention provides hydrogen-oxidizing bacteria, namely paracoccus denitrificans Y5, which is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms on May 12, 2017 (address: Institute of Microbiology of Chinese Academy of Sciences, No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, 100101), with preservation number CGMCC No. 14118. The paracoccus denitrificans Y5 provided by the invention can remove COD and ammonia nitrogen in high ammonia-nitrogen wastewater, fix production of single-cell protein (SCP) by CO2, and can remove stink of wastewater.

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

The invention relates to a treatment method for dephosphorizing and denitrifying sewage. The method is characterized by carrying out treatment by combining a physical method and a biological agent, wherein the biological agent comprises the following raw materials in parts by weight: 5-7 parts of paracoccus denitrificans, 5-7 parts of saccharomyces cerevisiae, 4-5 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 3-4 parts of clostridium papyrosolvens, 3-4 parts of penicillium ochrochloron, 2-3 parts of pseudomonas and 1-2 parts of bacillus cereus. The biological agent contains various microorganisms with excellent capacity of degrading refractory pollutants, is reasonable in compatibility of the strains, has good degrading effects and has a broad application prospect.

The invention discloses a compound microbiological preparation. The compound microbiological preparation comprises microorganisms and bio-enzyme, wherein the microorganisms contain bacillus subtilis,bacillus licheniformis, nitrosomonas, nitrosospira brieusis, P.Aeruginosa, paracoccus denitrificans, lactobacillus plantarum, lactobacillus casei, candida utilis, saccharomyces cerevisiae, rhodopseudomonas palustris, rhodospirillum, aspergillus niger and enterococcus faecalis; and the bio-enzyme contains microbial enzyme and an aerobic microbial agent. The compound microbiological preparation disclosed by the invention does not contain chemical substances, and secondary pollution to an environment is avoided, and therefore, the compound microbiological preparation is an environmental-friendlymicrobial agent. The preparation process of the compound microbiological preparation has the advantages that the preparation process is simple, the production cost is low, and the compound microbiological preparation has wide prospects on restoration of eutrophicated water bodies.

The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell 5 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methyl-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase. In some embodiments, the host cell is selected from a group of taxonimcal classes consisting of 20 Escherichia, Enterobacter, Azotobacter, Erwinia, Bacillus, Pseudomonas, Klebsiella, Proteus, Salmonella, Serratia, Shigella, Rhizobia, Vitreoscilla, Synechococcus, Synechocystis, and Paracoccus taxonomical classes. In some embodiments, the host cell is an Escherichia coli cell. In some embodiments, the host cell is a fungal cell, such as a yeast cell. In some embodiments, the yeast cell is a Saccharomyces sp. cell. In some embodiments, the host cell is an algal, insect or mammalian cell line. In some embodiments, the phosphatase is nudB from E. coli. In some embodiments, the IPP isomerase is encoded by an idi gene from E. coli or idil gene from Saccharomyces cerevisiae.

The invention provides a degradation strain for eliminating residual bromoxynil, belonging to the field of biological high technology. The adopted bacterial strain is a gram staining reaction negative strain MXX-04 which is identified to be paracoccussp. The main biological properties are G-, short rod shape, no spores, no motility and positive oxidase and catalase. The strain provided by the invention can grow by taking bromoxynil as a unique nitrogen source and mineralize the bromoxynil. Under the condition of a shake flask in a laboratory, the strain can degrade 95.3% of 100mg/L bromoxynil within 24 hours, thus the problem that bromoxynil is hard to degrade biologically in the environment is solved.

The invention belongs to the technical field of environmental protection and microorganisms, and finds and provides a set of screened and optimized rapid sewage organic matter degradation composite bacterial according to growth, functions and safety of microorganisms during biodegradation based on imperfection of the existing sewage treatment technology, and continuous amplification and applications of the corresponding effective bacterial so as to provide organic matter degradation efficiency in sewage treatment. The composite bacterial comprises bacillus cereus, bacillus subtilis, paracoccus denitrificans, bacillus badius, acinetobacter calcoaceticus, and bnfillus licheniformis. The present invention provides a rapid sewage organic matter degradation method, wherein sewage filled with a carrier is added with the composite bacteria, organic waste in the sewage can be synergistically degraded by protease, lipase and other decomposition enzymes produced by the composite bacterial, and a reduction rate of the organic matters can be about 80%. The invention further provides a method capable of amplifying effective bacterial in the existing organic sewage or the organic sewage to be treated so as to achieve high sewage treatment efficiency.

The invention discloses a method for treating nitrogen-containing wastewater by using aheterotrophic nitrification-aerobic denitrification paracoccus strain, wherein the nitrogen-containing wastewatercontains organic acid sodium, and the C/N ratio is 8-150. The method comprises: inoculating a heterotrophic nitrification-aerobic denitrification paracoccus seed liquid into ammonia nitrogen wastewater, and carrying out an oscillation reaction at a temperature of 25-40 DEG C at a rotating speed of 90-180rpmso as to finish the treatment of the ammonia nitrogen wastewater, wherein the strain is paracoccus LJ2, is preserved in the Guangdong culture collection center, has the preservation number ofGDMCC NO:60338, has extremely high capacity of high-concentration organic matter tolerance, and hasexcellent capacity of ammonia nitrogen removal, nitrate nitrogen removal and nitrite nitrogen removal under high organic load, so that the biological denitrification treatment of high-concentration organic matter nitrogen-containing wastewater by using paracoccus LJ2 has huge application prospect.

The invention relates to a composite microbial inoculum for harmless processing, and its application. The composite microbial inoculum for harmless processing comprises Paracoccus denitrificans, Bacillus megaterium, Bacillus subtilis, Bacillus badius, Bacillus thuringiensis, Bacillus licheniformis, Acinetobacter calcoaceticus, Bacillus laterosporus and Bacillus cereus. The composite microbial inoculum can be used for processing sewage, domestic organic wastes, sewage plant sludge, or human and animal excreta.

The invention provides a degradation bacillus which can dispel the long-lasting phosphorus pesticide residue and its producing bacillus. The strain is Gram's stain reaction hysteroptosis m-1 in the field of Paracoccus.sp.; the main biology characteristic is G-; the thaliana is the short rod type which likes oxygen and dislikes sport; the scavenger enzyme and the oxidative enzyme is positive; it can not use citrate and nitrate to reduction the positive; the denitrifying reaction generates the gas.

The invention discloses a combination of high-efficiency bacteria for sewage treatment, which comprises the following five kinds of microorganisms: Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Acinetobacter calcium acetate and paracoccus denitrification. The following process: sampling in the target environment, culture in the culture medium, isolation of the target strains, index analysis of the isolated strains, species identification, compatibility and optimization. The functional bacteria of the invention proliferate rapidly in the process of decomposing organic matter, and have obvious phosphorus removal effect on sewage, so that the treatment effect of sewage can reach the national discharge standard, and can greatly reduce sludge discharge.