47 results about "Paracoccus" patented technology

The present invention relates to a method for treating a bacterial infection by using Paracoccus wg1 T The invention relates to a method for treating wastewater containing high concentration of inorganic nitrogen, which belongs to the technical field of waste resource utilization and water environment treatment. T The seed liquid after culture is used to treat nitrogen-containing wastewater, and the Paracoccus wg1 of the present invention T , has a very high tolerance to high concentrations of inorganic nitrogen, and has excellent denitrification capabilities under conditions of high concentrations of inorganic nitrogen. Specifically, sodium nitrate is used as the nitrogen source, sodium propionate is used as the carbon source, NO3 ‑ When the ‑N concentration is 6258.85 mg / L‑N, 97.68 mg / L‑N of NO3 can be degraded in 48 hours ‑ -N; ammonium sulfate as nitrogen source, sodium propionate as carbon source, NH4 + When the ‑N concentration is 9326.28 mg / L‑N, 92.28 mg / L‑N of NH4 can be degraded in 48 hours + ‑N. paracoccus wg1 T Biological denitrification treatment of high-concentration inorganic nitrogen wastewater has great application prospects.

The invention discloses a culture medium for increasing the yield of paracoccus astaxanthin and application of the culture medium. The culture medium comprises the following components according to concentration: 4-20g / L of yeast extract; 8-20 g / L of beef extract; 4 to 20 g / L of bacteriological peptone; 10 to 30 g / L of glucose; the concentration of KH2PO4 is 0.1 to 0.2 g / L; the concentration of Na2HPO4. 12H2O is 0.1 to 0.5 g / L; 0.1 to 1 g / L of MgSO4. 7H2O; 0.1 to 1 g / L of FeSO4. 7H2O; the concentration of CaCl2. 2H2O is 0.02 to 0.1 g / L; the pH is 7.2 + / -0.2. The yeast extract, the beef extract and the bacteriological peptone are selected and mixed to serve as a nitrogen source, accumulation of paracoccus biomass and astaxanthin can be promoted synergistically, and meanwhile the problem of precipitation after the culture medium is sterilized is solved.

The application discloses a formic acid dehydrogenase mutant and application thereof in catalyzing carbon dioxide reduction. Paracoccus The formic acid dehydrogenase from sp.MKU1 is mutated by a site-directed mutation technology Ps The arginine (Arg) at the 223th position of the FDH48 amino acid sequence is mutated into proline (Pro) or the proline (Pro) at the 242th position is mutated into phenylalanine (Phe), and the obtained mutants R223P and P242F have higher catalytic activity, can more effectively catalyze CO2 to generate formic acid in vitro, and have potential application values in efficient activation of CO2 and further conversion into other one-carbon compounds through cascade reactions.

The invention provides a respiratory system fungal infection detection system, a kit and a preparation method thereof, and belongs to the technical field of biological detection. The primer probe composition used by the respiratory system fungal infection detection system comprises upstream and downstream primers and a fluorescent probe sequence designed aiming at paracoccus braziliensis, upstream and downstream primers and a fluorescent probe sequence designed aiming at histoplasma capsulatus, and upstream and downstream primers and a fluorescent probe sequence designed aiming at troptosporidium. The kit comprises upstream and downstream primers and fluorescent probe sequences designed for budgettes dermatitis, upstream and downstream primers and fluorescent probe sequences designed for Sirpolyspora sessilifolia, upstream and downstream primers and fluorescent probe sequences designed for talaromyces marneffei, upstream and downstream primers and fluorescent probe sequences designed for internal reference designed for ribonuclease P, upstream and downstream primers and fluorescent probe sequences designed for ribonuclease P, upstream and downstream primers and fluorescent probe sequences designed for ribonuclease P, and upstream and downstream primers and fluorescent probe sequences designed for ribonuclease P, the kit can be used for effectively detecting the fungi of the respiratory system, and ensures high accuracy, specificity and sensitivity.

Gas bio-trickling filter (g-BTF) processes are provided for polluted air purification mainly focusing on the treatment of VOCs and odor using low-cost filter media (agricultural waste i.e., wood char coal and other waste i.e., sludge, compost etc.) that is cheap and readily available. Apparatus for purifying volatile organic compounds and odor control enriched air stream uses a consortium of the microorganisms including Rhodococus sp., Paracocus sp, Microbacterium sp., Mutant Paracocus denitrificans sp. The consortium approach ensures a broader range of VOC degradation capabilities, allowing for efficient removal of multiple compounds simultaneously. This versatility makes biotrickling filters highly adaptable to different industrial settings, such as paint manufacturing plants or automotive facilities. Compared to other process (thermal and chemical process), the developed BTF process are associated with the lowest operating and maintenance costs. Overall VOC and odor removal efficiency of the g-BTF unit is 99.9%.

The invention discloses construction of an artificial multicellular system and application of the artificial multicellular system in nitrogen reduction, and belongs to the technical field of fermentative degradation. According to the artificial multi-cell system constructed by the method disclosed by the invention, a direct ammoxidation gene dnfABC is over-expressed by adopting Shinella zoogloeoides ATCC19623, and a urea degradation gene cluster ureDABCJEFG is over-expressed by adopting Paracoccus denrificans DYTN-1; the constructed recombinant bacteria are independently or jointly used for nitrogen reduction. According to the method, the synergistic effect of microorganisms in the nitrogen degradation process is utilized, the artificial multicellular system meeting the nitrogen pollution wastewater treatment requirement is developed, the degradation effect and the process stability are remarkably improved, and rapid degradation of nitrogen is achieved.

The invention relates to the technical field of paper cups, and discloses a compound bacterium for degrading nitrite in an aquaculture water body, the compound bacterium is composed of nitrifying bacteria, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist, the nitrifying bacteria are mixed flora of autotrophic ammonia oxidizing bacteria and heterotrophic nitrifying bacteria, and the penetrant is a mixture of bacillus coagulans, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist. The denitrifying bacteria comprise a synergistic effect system of the Pseudomonas (Pseudomonas) and the Paracoccus (Paracoccus), and the Pseudomonas (Pseudomonas) and the Paracoccus (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas)) are The dual-path directional conversion of nitrite is realized through the synergistic effect of constructed nitrification-denitrification synergistic flora and a functional aid (polyethylene glycol-SDS-L-glutamine system). Parameter control shows that in a water body (NO2 <-> is equal to 2.5 mg / L) in the later culture period of the penaeus vannamei boone, the degradation rate reaches 81.4% within 48 hours, and the nitrate increment is controlled within 12%. The technology breaks through the bottleneck of failure of a traditional microbial agent in a high-salinity (35 per mill) and high-density culture environment, and is suitable for sea and fresh water whole-variety aquaculture.

The invention relates to a biomarker for individual recognition and application thereof, and relates to the technical field of biology. The biomarker comprises at least one of the following microorganisms: propionibacterium acnes, staphylococcus epidermidis, marine paracoccus, streptococcus, rhodococcus fence celebrating, staphylococcus, pseudomonas, streptococcus sanguineus, achromobacter xylosoxidans, acinetobacter calcoaceticus, acinetobacter johnsonii, staphylococcus lentus, pseudomonas toralaris and brevundimonas vesicularis. Or enterococcus faecium. The biomarker can provide analysis with higher resolution in the aspect of differential strain information, has a better distinguishing effect, higher accuracy and sensitivity on individual recognition, and can be used as a new technical means for supplementing forensic individual recognition.

The application discloses Paracoccus communis C4-4 and C5-3, the preservation number of which is CGMCC NO:31971 and the classification name of which is Paracoccus communis, and the preservation number of which is CGMCC NO:31972 and the classification name of which is Paracoccus communis. Researches show that C4-4 and C5-3 have strong ecological adaptability and good growth promotion ability, and can improve the internal environment of soil in combination with organic materials. A synthetic microbial population constructed by Paracoccus communis comprises C4-4 and C5-3. Tests prove that the synthetic microbial population comprising C4-4 and C5-3 has the advantages of the synthetic microbial population, the microbial population has high alkali production and growth promotion ability, has good ecological adaptability, the pH adaptation range is 4-8, the manganese stress tolerance can reach 5000 μmol / L, can significantly improve the pH of a culture medium, and has obvious alkalization effect. The synthetic microbial population is used as a microbial agent and is compounded with organic materials to prepare a soil conditioner capable of improving acid soil and promoting plant growth. The conditioner is applied to acid and lean soil, can significantly improve the soil pH, improve the content of soil organic matter, nitrogen, phosphorus and potassium, and promote root growth.

The application provides a high-salt and high-nitrate nitrogen wastewater denitrification method, which comprises the following steps: S1, adjusting the pH of the high-salt and high-nitrate nitrogen wastewater to the range of 5.5-7.5, and adopting a primary full-mixed denitrification tank for denitrification, wherein a composite microbial inoculum is put into the primary full-mixed denitrification tank at one time, and the composite microbial inoculum comprises Marinobacter, Azoarcus, Paracoccus, Bacillus and Pseudomonas; S2, adopting a secondary full-mixed denitrification tank for denitrification of the effluent of step S1; during the operation of the secondary full-mixed denitrification tank, a mud-water mixture is backflowed to the primary full-mixed denitrification tank; S3, the effluent of step S2 is introduced into a degassing tank for degassing; S4, the effluent of step S3 is precipitated in a precipitation tank, and then the effluent is discharged. The composite microbial inoculum is used for treating the high-salt and high-nitrate nitrogen wastewater, and the problems of large treatment facility area and high cost caused by concentrated evaporation or dilution of wastewater and then biochemical treatment in traditional treatment are avoided.

The invention discloses paracoccus versutus and an application thereof, and particularly discloses paracoccus versutus and an application thereof, the paracoccus versutus is paracoccus versutus TYWJ-2, the preservation number is CGMCC No.31068, and the paracoccus versutus is preserved in the China General Microbiological Culture Collection Center on July 2, 2024, the preservation number is CGMCC No.31068, and the preservation number is CGMCC No.31068. The address is located in Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the postcode is 100101. The Paracoccus verrucosus TYWJ-2 provided by the invention has stronger desulfurization capacity and higher sulfur conversion rate, the 12h sulfide degradation rate reaches 98.1%, the sulfur generation rate reaches 76.8%, and the Paracoccus verrucosus TYWJ-2 has good biological desulfurization application potential.

The present invention discloses a method for strengthening pyridine degradation and synchronous short-term denitrification by combining functional bacteria with MABR process. According to the ecological niche characteristics of biofilm in MABR process, the present invention sequentially forms biofilms with rhizobium NJUST18 inoculum of pyridine degradation functional strain, nitrifying sludge and denitrifying functional strain Paracoccus denitrifying NJUST53 inoculum. The rhizobium in the aerobic layer of the biofilm formed after biofilm formation uses pyridine as the only carbon source and nitrogen source for growth, and the nitrifying bacteria use the ammonia nitrogen produced by pyridine degradation to carry out the nitrification process. The denitrifying Paracoccus in the anoxic layer uses the nitrite nitrogen / nitrate nitrogen and pyridine produced in the nitrification process to carry out denitrification. In addition, the activity of nitrite oxidizing bacteria is suppressed by controlling the aeration pressure and the pH of the influent, thereby achieving the strengthening of pyridine degradation and synchronous short-term denitrification.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The invention relates to the technical field of microbial denitrification, and discloses paracoccus denitrificans and application thereof. The paracoccus denitrificans is named as 392A9 and is preserved in the China General Microbiological Culture Collection Center on October 20, 2023, the preservation number of the paracoccus denitrificans is CGMCC No.28691, and the classification of the microorganisms of the paracoccus denitrificans is named as paracoccus denitrificans. The paracoccus denitrificans 392A9 provided by the invention can survive and realize efficient denitrification under aerobic and anaerobic conditions and higher nitrate concentration, and can adapt to a wider pH (Potential of Hydrogen) range, so that the paracoccus denitrificans 392A9 has a better application prospect in denitrification treatment of nitrate wastewater.

The invention belongs to the technical field of biology, and provides a paracoccus Wheatstone strain and application thereof, the paracoccus Wheatstone strain can produce pyrroloquinoline quinine at high yield, the strain is named as YJY25-02, and the preservation number is CGMCC NO.34258. The strain is subjected to shake-flask aerobic culture for 83h, and the titer of the pyrroloquinoline quinone in fermentation liquor is 176.7 mg / L; the titer of pyrroloquinoline quinine in fermentation liquor during aerobic culture in a fermentation tank is 1796-1863 mg / L, the methanol conversion rate is 16.19-18.12 mg / g, the method has the advantages of short fermentation time, high titer and high methanol conversion rate, and the problem of large-scale production can be solved; pure pyrroloquinoline quinine with the purity larger than or equal to 98% is prepared through separation and purification of fermentation liquor, the yield ranges from 80% to 85%, the high yield and high purity of the pure pyrroloquinoline quinine are achieved, the separation and purification process is simple, the cost is low, organic solvents difficult to remove are not introduced, and the safety of the product is guaranteed.

The invention belongs to the technical field of biological medicine, and particularly relates to a respiratory tract allergic inflammation relieving agent based on environmental microorganism extracts and application of the respiratory tract allergic inflammation relieving agent. According to the invention, the application of the paracoccus iili in preparation of the product for preventing and treating the chronic respiratory disease is found for the first time, and the paracoccus iili as a purely natural and pollution-free environmental beneficial bacterium does not destroy the balance of microorganisms in an indoor environment, can generate beneficial metabolites, and is beneficial to reducing the risk of the respiratory disease. Cell and animal experiments find that the paracoccus Iili can reduce asthma-related inflammatory factors, directly acts on respiratory mucosa through inhalation exposure, can play a role in preventing and protecting respiratory diseases, and has a good development value. The asthma prevention and treatment product based on paracoccus Israei can be combined with balance maintenance of microbial communities, and a comprehensive and sustainable respiratory disease risk prevention effect is provided for the indoor environment.

The invention discloses a method for producing polyhydroxyalkanoate through microbial fermentation and application of the polyhydroxyalkanoate. The method for producing the polyhydroxyalkanoate through microbial fermentation comprises the following steps: optionally adding water into organic acid feed liquid for dilution, and then adding a complex microbial inoculant containing flavobacterium for aerobic fermentation to obtain PHA-rich fermentation thalli; the complex microbial inoculant comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by carrying out acid production fermentation on a pretreated material derived from lignocellulose. According to the method, the lignocellulose is used as a raw material to replace a grain raw material to produce the organic acid under the condition of no enzymolysis. The microbial agent disclosed by the invention contains neosilurus meridionalis, dearomatization bacteria, flavobacterium, flavobacterium and paracoccus, the strains act together, the organic acid can be effectively utilized for further fermentation to generate polyhydroxyalkanoate, and the microbial agent has important economic and environmental benefits.

The present invention relates to the technical field of nitrate nitrogen wastewater treatment, and discloses a microbial treatment method for high nitrate nitrogen wastewater, comprising the following steps: adding a microbial carrier to a wastewater treatment device, and then inoculating denitrifying Paracoccus with a preservation number of CGMCC No.28691 ( Paracoccus denitrificans )392A9, and biofilm formation; the nitrate nitrogen content in the wastewater is controlled to be no higher than 11000 mg / L, the pH is controlled to be 5.0-8.0, and the wastewater is passed into the wastewater treatment device for microbial denitrification. The method of the present invention can effectively reduce the nitrate nitrogen concentration in high nitrate nitrogen wastewater, simplify the wastewater pretreatment process, reduce the amount of dilution water, and can achieve effective treatment of high nitrate nitrogen wastewater under both anaerobic and aerobic conditions.

The invention discloses paracoccum C4-4 and C5-3, the preservation number of the paracoccum C4-4 and C5-3 is CGMCC (China General Microbiological Culture Collection Center) NO: 31971, the paracoccum C5-4 and C5-3 are classified and named as Paracoccus communis, and the preservation number of the paracoccum C4-4 and C5-3 is CGMCC NO: 31972, and the paracoccum C5-3 is classified and named as Paracoccus communis. Researches show that C4-4 and C5-3 both have strong ecological adaptability and good growth promoting ability, and can improve the environment in the soil by combining with organic materials. The synthetic flora constructed by using the paracoccus comprises the C4-4 and the C5-3. Tests prove that the synthetic flora has the advantages of C4-4 and C5-3, and the flora has higher alkali production and growth promotion capabilities; good ecological adaptability is achieved, the pH adaptation range is 4-8, and manganese stress resistance can reach 5000 micromoles per liter; the pH of the culture medium can be obviously improved, and the alkalization effect is obvious. The soil conditioner capable of improving acid soil and promoting plant growth is prepared by taking the synthetic flora as a microbial inoculum and compounding the microbial inoculum with an organic material. When the modifier is applied to acid and lean soil, the soil pH can be remarkably increased, the content of organic matters and nitrogen phosphorus and potassium in the soil is increased, and root growth is promoted.

This invention relates to a phthalate hydrolase mutant and its application in phthalate degradation, belonging to the field of microbial enzyme technology. The amino acid sequence of the phthalate hydrolase mutant HylD1 is shown in SEQ ID NO.1, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.2. This enzyme can catalyze the reaction to generate MMP using DMP as a substrate; or catalyze the reaction to generate MEP and EMP using DEP as a substrate. In this invention, the encoding gene of the phthalate hydrolase HylD1 from *Paracoccus koningii* BJQ0001 was artificially modified by site-directed mutagenesis at amino acid position 265, resulting in a mutant HylD1 (R265A) with higher activity. Compared with the wild-type phthalate hydrolase HylD1, the absolute enzyme activity of this phthalate hydrolase mutant HylD1 (R265A) in degrading DMP and DEP is increased by 2-fold and 3.6-fold, respectively, and a special product EMP is also produced during the degradation of DEP.

The application discloses a synthetic bacterial flora capable of improving salt tolerance of alfalfa, which is composed of Pseudomonas strain EC5, Agrobacterium strain EC17, Streptomyces strain EC15, Paracoccus strain RH39, Pseudomonas strain RH6, Pseudomonas strain RH37, Sporosarcina strain RH34 and Bacillus strain RH32. The synthetic bacterial flora has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing siderophore, producing biofilm, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, plumule length and fresh weight of the group seedling embryo of alfalfa. Under the stress of 200 mmol / L NaCl, seed soaking with the synthetic bacterial flora can significantly improve the plant height, stem diameter, leaf area and fresh and dry weight of the above-ground part of alfalfa.

The invention discloses a paracoccus denitrifying nitrogen removal device which comprises a box body, the box body is connected with a water inlet channel for sewage to enter and a water outlet channel for purified water to be discharged, a rotary drum capable of rotating relative to the box body is arranged in the box body, the cylindrical surface of the rotary drum is hollow, and a biochemical filling block is placed in the cylindrical surface of the rotary drum; an aeration pipeline is arranged in the box body, the box body is at least provided with an air outlet structure communicated with the aeration pipeline on one side of the rotating axis of the rotating drum, and the air outlet structure is positioned below the rotating drum, so that the air outlet structure can push the rotating drum to rotate while spraying bubbles to supply oxygen to sewage. The invention provides a paracoccus denitrifying nitrogen removal device, so as to provide an environment suitable for living of paracoccus, improve the activity of paracoccus and improve the nitrogen removal efficiency.

The invention provides a method for strengthening azo dye decolorization based on co-culture of shewanella and paracoccus denitrificans, and relates to the technical field of microorganism co-culture and bioremediation. The shewanella can reduce various pollutants through extracellular electron transfer, but cannot effectively utilize acetate as a carbon source. Paracoccus denitrificans can efficiently oxidize acetate, but lacks the ability to deliver electrons to extracellular electron acceptors (such as azo dyes). According to the method, the S. oneidensis MR-1 in a stable phase and the P. denitrifans in a logarithmic growth middle phase are co-cultured according to a certain biomass ratio, so that the anaerobic decolorization efficiency on various azo dye pollutants can be remarkably enhanced. By constructing a symbiotic system based on functional complementation, a new strategy with potential is provided for enhanced removal of azo dye pollution.

The present invention provides a strain of Paracoccus mutans capable of degrading benzo[a]pyrene. The provided Paracoccus mutans strain LYCW-1-6 has a deposit number of CGMCC No. 24841. The Paracoccus mutans provided by the present invention can be used to degrade benzo[a]pyrene. When the benzo[a]pyrene-degrading bacteria is inoculated at a ratio of 2% into a liquid culture medium containing 100 mg / L of benzo[a]pyrene, after 7 days of culture, the benzo[a]pyrene in the culture medium can be degraded by up to 70%. Furthermore, compared to other reported benzo[a]pyrene-degrading bacteria, the strain of the present invention is capable of treating soil or water contaminated with high concentrations of benzo[a]pyrene.

The invention discloses a construction and analysis method of a paracoccus denitrificans genome scale metabolic network model, and belongs to the technical field of system biology. The method comprises the following steps: annotating a whole genome; acquiring global metabolic reaction data of paracoccus denitrificans; the method comprises the following steps: automatically retrieving genome information and constructing a model 1 on the basis of species codes and genome annotation results of paracoccus denitrificans in a KEGG database; on the basis of a pre-trained hidden Markov model, homology search is carried out on protein of a target organism, homologous protein in a paracoccus denitrificans genome is identified, additional metabolic reaction is added, and a model 2 is constructed; and integrating the model 1 and the model 2. According to the method, denitrification engineering can be efficiently designed and modified, accurate regulation and control of the nitrogen degradation process are achieved, compared with an existing metabolic engineering modification method, the workload of an exploration experiment can be effectively reduced, and the method has a great promotion effect on deepening the nitrogen degradation characteristic of paracoccus denitrificans.

The invention relates to the technical field of aquatic bait culture, in particular to an indoor large-scale amplification culture method for moina mongolica. Comprising the steps that an oxygen pipe, a temperature control pipe and an air stone are arranged in a culture pond, a double-oxygen oxygenation system is placed in the middle, and an incandescent lamp is arranged above; disinfecting and scrubbing facilities in the pool and pool walls with bleaching water; sequentially carrying out rough filtration, fine filtration and ultraviolet disinfection on external seawater, mixing the external seawater with tap water, adjusting the salinity to 15-20, and adding a sodium hypochlorite solution for disinfection; performing detoxification with sodium thiosulfate; screening and expanding germplasm of the moina mongolica by using a 50-60-mesh bolting-silk net; inoculating moina mongolica in the culture water body according to the density of 5500-6500 moina mongolica per liter; environment and water quality parameters are manually regulated and controlled after inoculation; the concentrated nannochloropsis oculata and the yeast are mixed for feeding, and when the daphnia magna density reaches 18000-23000 daphnia magna / L, all the daphnia magna is harvested at a water outlet. The method has the advantages that the daphnia body amplification density is high, and the amplification period is shortened; and pathogenic microorganisms are few.

The invention relates to a complex microbial inoculant for alkali-minimization wastewater treatment as well as a preparation process and application thereof. The complex microbial inoculant is prepared from the following active components in parts by weight: 10 to 12 parts of paracoccus, 9 to 10 parts of hyphomicrobium, 7 to 8 parts of polynematode tarda, 7 to 8 parts of Fabry-Perot, 5 to 6 parts of rope warming bacteria and 2 to 3 parts of spirillum rot. According to the application, a plurality of new strains are formed in the complex microbial inoculant which is cultured by mixing the basic microbial inoculant, the anaerobic sludge, the glucose and the phosphate fertilizer, and the newly generated strains cooperate to enable the complex microbial inoculant to accidentally obtain the performance of efficiently degrading alkali-minimization wastewater pollutants in a high-alkali environment.

The invention discloses a method for preparing PHA (polyhydroxyalkanoate) by using a complex microbial inoculant and application of the PHA. The method for preparing the PHA by using the complex microbial inoculant comprises the following steps: adding the complex microbial inoculant containing flavobacterium into a to-be-fermented liquid, and carrying out aerobic fermentation to obtain a PHA-rich fermented thallus; the liquid to be fermented contains organic acid feed liquid and optional water; the complex microbial inoculant disclosed by the invention comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by performing acid production fermentation on raw materials of enzymatic hydrolysate containing pentose and hexose. According to the present invention, the composite bacterial agent with the high organic acid utilization rate is prepared, and the multiple organic acid fermentation liquids obtained from lignocellulose can be effectively utilized by using the composite bacterial agent so as to perform PHA synthesis, such that the large-scale production of PHA and the resource utilization of lignocellulose are easily achieved.

The application discloses a culture medium for improving production of paracoccus astaxanthin and application thereof. The culture medium comprises the following components according to concentration calculation: yeast extract 4-20 g / L; beef extract 8-20 g / L; bacteriological peptone 4-20 g / L; glucose 10-30 g / L; KH2PO4 0.1-0.2 g / L; Na2HPO4.12H2O 0.1-0.5 g / L; MgSO4.7H2O 0.1-1 g / L; FeSO4.7H2O 0.1-1 g / L; CaCl2.2H2O 0.02-0.1 g / L; and pH 7.2+ / -0.2. In the application, the yeast extract, beef extract and bacteriological peptone are mixed as the nitrogen source, which can promote the accumulation of paracoccus biomass and astaxanthin, and meanwhile, the problem of precipitation after sterilization of the culture medium is solved.

The application provides a composite microbial agent special for sewage denitrification and a carbon source preparation method thereof, and belongs to the technical field of sewage treatment. The composite microbial agent comprises a mixed bacterial solution composed of iron-oxidizing autotrophic denitrifying bacteria, heterotrophic nitrification-aerobic denitrification bacteria and iron mineral mineralization associated bacteria; the iron-oxidizing autotrophic denitrifying bacteria are Paracoccus ferrooxidans, the heterotrophic nitrification-aerobic denitrification bacteria are Pseudomonas stutzeri, and the iron mineral mineralization associated bacteria are Shewanella. The triple coupling system of autotrophic denitrification, heterotrophic denitrification and iron mineral mineralization is constructed, and the industry pain points of insufficient carbon source of low-carbon sewage, poor low-temperature denitrification, easy loss of bacterial strains and high cost of chemical carbon source are solved.