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Paracoccus denitrifican with denitrification and iron reduction functions and culturing method and application thereof

A technology for denitrifying paracoccus and its cultivation method, which is applied in the field of denitrifying paracoccus ZGL1 and its cultivation, which can solve the problems of increased operating costs and achieve the effects of less investment and operating costs, strong practical value, and simple processes

Inactive Publication Date: 2012-09-19
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the treatment of low C / N sewage, a large amount of additional organic matter needs to be added to meet the process requirements, which will lead to an increase in operating costs

Method used

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  • Paracoccus denitrifican with denitrification and iron reduction functions and culturing method and application thereof
  • Paracoccus denitrifican with denitrification and iron reduction functions and culturing method and application thereof
  • Paracoccus denitrifican with denitrification and iron reduction functions and culturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Denitrification of heterotrophic denitrification wastewater by strains of the present invention under organic carbon source conditions

[0022] Organic carbon source denitrification medium: MgSO 4 ·7H 2 O 5 mM, CaCl 2 2H 2 O 5 mM, NH 4 Cl 0.28 g / L, KH 2 PO 4 0.25 g / L, NaHCO 3 5.4 g / L, KNO 3 10 mM, glucose 5 mM, adjust the pH to 7.2~7.4, and the culture medium is sterilized.

[0023] Using an organic carbon source denitrification medium, inoculate the strain ZGL1 with a dry weight of 0.6 g / L under anaerobic conditions and cultivate it at 30°C. Samples were taken at regular intervals to determine the change of nitrate concentration. The results are shown in Table 1. It can be seen from Table 1 that in the presence of organic carbon sources, the denitrification speed is fast and the efficiency is high. After 24 hours of cultivation, 94.9% of the nitrate in the system was removed.

[0024] Table 1 Effect of strain ZGL1 on heterotrophic denitrificat...

Embodiment 2

[0026] Example 2: Simultaneous heterotrophic denitrification waste gas denitrification and iron reduction by the strain of the present invention under the condition of organic carbon source

[0027] Organic carbon source denitrification iron reduction medium: MgSO 4 ·7H 2 O 5 mM, CaCl 2 2H 2 O 5 mM, NH 4 Cl 0.28 g / L, KH 2 PO 4 0.25 g / L, NaHCO 3 5.4 g / L, glucose 5 mM, Fe II EDTA-NO 4 mM, Fe III EDTA 10mM, adjust the pH to 7.2~7.4, and the culture medium is sterilized.

[0028] Use the organic carbon source denitrification iron reduction medium, inoculate the strain ZGL1 with a dry weight of 0.6g / L under anaerobic conditions, cultivate it at 30°C, and take samples at regular intervals to measure Fe II EDTA-NO and Fe III The changes of EDTA concentration, the results are shown in Table 2. It can be seen from Table 2 that after 12 h of cultivation, Fe II EDTA-NO and Fe III EDTA was well reduced, Fe II EDTA-NO reduction rate is 80.3%, Fe III EDTA is 55.8%. Strain ...

Embodiment 3

[0031] Embodiment 3: Denitrification of sulfur autotrophic denitrification wastewater by bacterial strains of the present invention

[0032] Sulfur autotrophic denitrification medium: MgSO 4 ·7H 2 O 5 mM, CaCl 2 2H 2 O 5 mM, NH 4 Cl 0.28 g / L, KH 2 PO 4 0.25 g / L, NaHCO 3 5.4 g / L, KNO 3 10 mM, Na 2 S 2 o 3 ·5H 2 O 5 mM, adjust the pH to 7.2~7.4, and the culture medium is sterilized.

[0033] Use the organic carbon source denitrification iron reduction medium, inoculate the strain ZGL1 with a dry weight of 0.6g / L under anaerobic conditions, cultivate it at 30°C, and take samples at regular intervals to measure NO 3 - and S 2 o 3 2- The changes in concentration are shown in Table 3. It can be seen from Table 3 that strain ZGL1 can not only grow under heterotrophic conditions, but also grow under autotrophic conditions. The initial concentration was 10 mM NO 3 - and 5 mM S 2 o 3 2- , strain ZGL1 can carry out sulfur autotrophic denitrification process, aft...

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Abstract

The invention belongs to the technical field of environmental organisms, and relates to paracoccus denitrifican with heterotrophic denitrification, autotrophic denitrification and iron reduction functions and a culturing method and an application thereof. The bacterium is paracoccus denitrifican ZGL1 with the collection number CCTCC M2012158, and can be used for completing heterotrophic denitrification and autotrophic denitrification processes (including sulfur autotrophic denitrification and iron autotrophic denitrification) and effectively reducing ferric iron. During waste gas treatment, reduction of Fe(II)EDTA-NO and regeneration of Fe(II)EDTA are realized simultaneously for the same strain in a BioDeNOX denitration process, the aim of continuously denitrating is fulfilled, and low energy consumption, small investment and low running cost are realized. During waste water treatment, the strain can be used for removing nitrate and nitrite from waste water in a heterotrophic denitrification process, can be applied to waste water denitrification through sulfur autotrophic denitrification and iron autotrophic denitrification processes, is low in cost, and has a simple process.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and specifically relates to a denitrifying paracoccus ZGL1 with functions of heterotrophic denitrification, autotrophic denitrification (iron autotrophic denitrification and sulfur autotrophic denitrification) and iron reduction and its cultivation method and application. Background technique [0002] In recent years, my country has made remarkable achievements in air pollution control, the emission of total particulate matter has been basically controlled, SO 2 emissions have declined, but nitrogen oxides (NO X ) emissions remain high, and with the continuous development of my country's economy and the growth of energy consumption, there is an upward trend. During the "Eleventh Five-Year Plan" period, with NO X The continuous aggravation of pollution has seriously affected my country's economic development and people's lives. Therefore, during the "Twelfth Five-Year Plan" period, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/56C02F3/34C12R1/01C02F101/16
CPCY02A50/20
Inventor 张玉李宁董西洋陈明翔周集体
Owner DALIAN UNIV OF TECH
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