A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial
A technology of organic sewage and complex flora, applied in the direction of bacteria, biological water/sewage treatment, and methods based on microorganisms, can solve the problems of large differences in sewage biodegradation effects, repeated phenomena of biodegradable sewage effects, and complex components. Achieve the effects of improving sewage treatment efficiency, expanding treatment and scope, and enhancing the adaptability of bacterial flora
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Embodiment example 1
[0053] The preparation of implementation case 1 composite bacterial agent
[0054] 1) Fermentation and cultivation of various bacteria
[0055] Medium: LB
[0056] Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Bacillus sphaericus, Acinetobacter calcolacetate, and Paracoccus denitrifica were respectively sterilized under the condition of no bacteria (ultra-clean workbench) and slanted shake flask strains. Bacteria were cultured separately in LB liquid medium.
[0057] Culture conditions: the temperature of the inoculation bed is controlled at 37°C, and the concentration of the shake flask culture solution is more than 1-100 billion / ml.
[0058] According to the evaluation of water quality, the bacteria were mixed according to the weight ratio to prepare a composite bacterial agent, and the prepared composite bacterial agent was stored at 4 degrees Celsius. For long-distance transportation or long-term storage, the suitable bacterial agent and auxiliary material...
Embodiment example 2
[0059] Implementation Case 2 Preparation and use of strain expansion device
[0060] Processing steps:
[0061] 1) Take a large tank, attach it to the surface for heating, and have an aeration box inside the tank, which can control the water in and out, adjust the aeration, and the temperature control operation box;
[0062] 2) Extract on-site organic sewage and fill it into tanks with a volume of 85% V, add industrial-grade medium (LB or potato medium), adjust the temperature at 37°C, and aerate.
[0063] 3) After cultivating for about 70 hours (observed by turbidity or 721-type spectrophotometer), when the peak is higher, divide the 85% solution of internal volume into the aerobic pool in the sewage treatment system.
[0064] 4) Pump in sewage again to fill the volume of 85% V, add culture medium, adjust temperature, aerate (37°C), repeat, so that the sewage treatment effect meets the design requirements.
Embodiment example 3
[0065] Implementation Case 3 Functional Test of Compound Bacteria and Amplification Device Enrichment Technology to Degrade Sewage
[0066]
[0067] Indicators and detection methods:
[0068] COD, SS, TN, TP, NH3-N are detected by national or industry standards:
[0069] 1. Water Quality - Determination of Chemical Oxygen Demand (Dichromate Method) GBT11914-89
[0070] 2. Water Quality - Determination of Suspended Solids (Gravimetric Method) GBT11901-89
[0071] 3. Water quality - Determination of total nitrogen GBT1189489
[0072] 4. Water quality - Determination of total phosphorus GBT11893-89
[0073] 5. Water Quality - Determination of Ammonia Nitrogen HJT195-2005.
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