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A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial

A technology of organic sewage and complex flora, applied in the direction of bacteria, biological water/sewage treatment, and methods based on microorganisms, can solve the problems of large differences in sewage biodegradation effects, repeated phenomena of biodegradable sewage effects, and complex components. Achieve the effects of improving sewage treatment efficiency, expanding treatment and scope, and enhancing the adaptability of bacterial flora

Inactive Publication Date: 2013-08-21
复环生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the treatment of organic sewage mostly adopts biodegradation-related technologies, that is, the sludge produced in the corresponding sewage treatment process is used as the original source of bacteria, and it is put into anaerobic and aerobic pools. After 3 to 5 months (temperature difference impact cycle) into normal operation, due to the different sources of sludge, sewage conditions, and corresponding processes, the effect of sewage biodegradation varies greatly, and sludge is the original source of biodegradation bacteria, and its composition is extremely complex, which is difficult Grasp, so that sometimes there is a repeated phenomenon of biodegradable sewage effect

Method used

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  • A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial
  • A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial
  • A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0053] The preparation of implementation case 1 composite bacterial agent

[0054] 1) Fermentation and cultivation of various bacteria

[0055] Medium: LB

[0056] Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Bacillus sphaericus, Acinetobacter calcolacetate, and Paracoccus denitrifica were respectively sterilized under the condition of no bacteria (ultra-clean workbench) and slanted shake flask strains. Bacteria were cultured separately in LB liquid medium.

[0057] Culture conditions: the temperature of the inoculation bed is controlled at 37°C, and the concentration of the shake flask culture solution is more than 1-100 billion / ml.

[0058] According to the evaluation of water quality, the bacteria were mixed according to the weight ratio to prepare a composite bacterial agent, and the prepared composite bacterial agent was stored at 4 degrees Celsius. For long-distance transportation or long-term storage, the suitable bacterial agent and auxiliary material...

Embodiment example 2

[0059] Implementation Case 2 Preparation and use of strain expansion device

[0060] Processing steps:

[0061] 1) Take a large tank, attach it to the surface for heating, and have an aeration box inside the tank, which can control the water in and out, adjust the aeration, and the temperature control operation box;

[0062] 2) Extract on-site organic sewage and fill it into tanks with a volume of 85% V, add industrial-grade medium (LB or potato medium), adjust the temperature at 37°C, and aerate.

[0063] 3) After cultivating for about 70 hours (observed by turbidity or 721-type spectrophotometer), when the peak is higher, divide the 85% solution of internal volume into the aerobic pool in the sewage treatment system.

[0064] 4) Pump in sewage again to fill the volume of 85% V, add culture medium, adjust temperature, aerate (37°C), repeat, so that the sewage treatment effect meets the design requirements.

Embodiment example 3

[0065] Implementation Case 3 Functional Test of Compound Bacteria and Amplification Device Enrichment Technology to Degrade Sewage

[0066]

[0067] Indicators and detection methods:

[0068] COD, SS, TN, TP, NH3-N are detected by national or industry standards:

[0069] 1. Water Quality - Determination of Chemical Oxygen Demand (Dichromate Method) GBT11914-89

[0070] 2. Water Quality - Determination of Suspended Solids (Gravimetric Method) GBT11901-89

[0071] 3. Water quality - Determination of total nitrogen GBT1189489

[0072] 4. Water quality - Determination of total phosphorus GBT11893-89

[0073] 5. Water Quality - Determination of Ammonia Nitrogen HJT195-2005.

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Abstract

The invention belongs to the technical field of environmental protection and microorganisms, and finds and provides a set of screened and optimized rapid sewage organic matter degradation composite bacterial according to growth, functions and safety of microorganisms during biodegradation based on imperfection of the existing sewage treatment technology, and continuous amplification and applications of the corresponding effective bacterial so as to provide organic matter degradation efficiency in sewage treatment. The composite bacterial comprises bacillus cereus, bacillus subtilis, paracoccus denitrificans, bacillus badius, acinetobacter calcoaceticus, and bnfillus licheniformis. The present invention provides a rapid sewage organic matter degradation method, wherein sewage filled with a carrier is added with the composite bacteria, organic waste in the sewage can be synergistically degraded by protease, lipase and other decomposition enzymes produced by the composite bacterial, and a reduction rate of the organic matters can be about 80%. The invention further provides a method capable of amplifying effective bacterial in the existing organic sewage or the organic sewage to be treated so as to achieve high sewage treatment efficiency.

Description

technical field [0001] The invention belongs to the field of environmental protection and microbiological technology, and in particular relates to microbial degradation of organic waste water microbial flora, and corresponding continuous amplification and application of effective bacteria in organic waste water. technical background [0002] At present, the treatment of organic sewage mostly adopts biodegradation-related technologies, that is, the sludge produced in the corresponding sewage treatment process is used as the original source of bacteria, and it is put into anaerobic and aerobic pools. After 3 to 5 months (temperature difference impact cycle) into normal operation, due to the different sources of sludge, sewage conditions, and corresponding processes, the effect of sewage biodegradation varies greatly, and sludge is the original source of biodegradation bacteria, and its composition is extremely complex, which is difficult Grasp, so that sometimes the biodegrada...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/125C12R1/085C12R1/10C12R1/07C12R1/01
Inventor 常莎孙栋林向勇
Owner 复环生物科技(上海)有限公司
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