149 results about "Paracoccus denitrificans" patented technology

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.

The invention relates to aerobic denitrifying paracoccus denitrificans and application thereof. A bacterial strain provided in the invention is paracoccus denitrificans DN-3CGMCC No. 3658; the Paracoccus denitrificans can carry out aerobic denitrification by using nitrate nitrogen under both aerobic and oxygen-limited conditions and can also carry out heterotrophic nitrification-aerobic denitrification by using ammonia-N, and a total nitrogen removal rate on above mentioned occasions is greater than 90%. The paracoccus denitrificans provided in the invention has stable hereditary features, isapplicable to treatment of a variety of nitrogen-containing waste water and produces a good nitrogen removal effect.

The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg/L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.

The invention relates to a biological agent for processing industrial sewage, a preparation method and application. The biological agent is prepared by the following raw materials in percentage by weight; 25% of rhodococcus, 20% of bacillus subtilis, 15% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 10% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the trains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by conventional activated sludge process.

The invention belongs to the technical field of environmental organisms, and relates to paracoccus denitrifican with heterotrophic denitrification, autotrophic denitrification and iron reduction functions and a culturing method and an application thereof. The bacterium is paracoccus denitrifican ZGL1 with the collection number CCTCC M2012158, and can be used for completing heterotrophic denitrification and autotrophic denitrification processes (including sulfur autotrophic denitrification and iron autotrophic denitrification) and effectively reducing ferric iron. During waste gas treatment, reduction of Fe(II)EDTA-NO and regeneration of Fe(II)EDTA are realized simultaneously for the same strain in a BioDeNOX denitration process, the aim of continuously denitrating is fulfilled, and low energy consumption, small investment and low running cost are realized. During waste water treatment, the strain can be used for removing nitrate and nitrite from waste water in a heterotrophic denitrification process, can be applied to waste water denitrification through sulfur autotrophic denitrification and iron autotrophic denitrification processes, is low in cost, and has a simple process.

The invention discloses a denitrogenation paracoccus strain and the cultivating method and the application. The strain is Paracoccus denitrificans W12 CGMCC No.1673. It has the strong degradation ability to organic pollutant, and could be used to take the biology treatment to the polluted water containing benzene, xylene, quinoline and cyanide and to the polluted soil. Especially, it has high degradation activity to benzene.

The invention relates to a microorganism bacterium agent for treating industrial wastewater pollution. The microorganism bacterium agent is prepared from pseudomonas putida, candida utilis, bacillus amyloliquefaciens, clostridium papyrosolvens, nitrosomonas europaea, paracoccus denitrificans and auxiliary materials. The microorganism bacterium agent has the advantages that various bacteria in mutualistic symbiosis are compatible reasonably without antagonism and are effective in degradation of phenyl amines degradation-resistant compounds when being thrown into a wastewater treatment system, thereby being suitable for industrial wastewater treatment and being capable of increasing treatment water quantity, improving treatment water quality, reducing operational cost and promoting up-to-standard discharge.

The invention discloses paracoccus and the application for degrading azotic heterocyclic compound. The paracoccus is paracoccus sp. BW001 CGMCC No. 2225. The paracoccus sp. BW001 CGMCC No. 2225 of the invention, on one hand, can grow into pyridine as the only carbon source and nitrogen source, which has a strong degradation activity (the degradation rate is 100%) for pyridine, is extremely suitable for biologic control of organic wastewater (such as coking wastewater) which contains pollutant of pyridine category, and can be used as biological hardening agent in the biological control technology of organic persistent wastewater, and corresponding environmental biological agent is exploited, thereby having relatively high value of study, application and marketing.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention relates to a cultural method for paracoccus denitrificans and application of the same to purifying aquaculture water, and belongs to the technical field of aquaculture. Various low-value materials such as molasses and waste liquid of monosodium glutamate and production waste which are harmless for aquaculture organisms and the paracoccus denitrificans are used as raw materials, the raw materials are reasonably compounded into liquid culture media, paracoccus denitrifican liquid with high activity and high concentration is produced, the liquid is directly fed into the aquaculture water, an ecological environment of the aquaculture water is repaired, and an excellent water environment beneficial to survival and growth is created for the aquaculture organisms. The culture method and the application have the advantages that the paracoccus denitrificans become effective fast after being used, an inorganic nitrogen removal form and the removal speed can be selected according to an oxygen dissolving condition of the water, contents of harmful substances such as ammonia nitrogen, nitrite and nitrate in the water are remarkably reduced, the quality of the water environment and a survival condition for the aquaculture organisms are improved, the yield of the aquaculture organisms is increased, and the quality of the aquaculture organisms is improved.

The invention belongs to the field of wastewater treatment and discloses a treatment process applicable to industrial wastewater. The treatment process applicable to the industrial wastewater comprises the following steps: 1) preparing complex microbial inoculant; 2) precipitating wastewater; 3) purifying the wastewater; 4) preparing bacterial manure. The prepared complex microbial inoculant comprises 5-6 parts of micrococcus luteus, 4-5 parts of bacillus mucilaginosus, 3-4 parts of paracoccus denitrificans, 2-3 parts of rhodococcus, 2-3 parts of acidithiobacillus ferrooxidans, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus cereus. The invention also discloses bacterial manure prepared by utilizing the treatment process applicable to the industrial wastewater. The treatment process applicable to the industrial wastewater is simple and feasible, the industrial wastewater can be effectively treated, and the bacterial manure is prepared by utilizing the treatment process applicable to the industrial wastewater.

The invention discloses a domestic sewage treatment microbial inoculant which comprises polyacrylamide, polyethyleneimine, silicon dioxide and compound microorganisms, wherein the compound microorganisms include Bacillus subtilis, Pseudomonas aeruginosa, Bacillus cereus, Candida lipolytica, nitrobacteria and Paracoccus denitrificans. The treatment agent can effectively treat domestic sewage, is low in cost and favorable in effect, and reduces pollution of the domestic sewage to the ecological environment; the sewage suspended substance removal rate is up to 99.8%, the BOD (biochemical oxygen demand) removal rate is 98%, the COD (chemical oxygen demand) removal rate is 98.6%, the chroma is 5, and the sludge quantity is so low that no sludge treatment system needs to be arranged; and the domestic sewage treatment microbial inoculant causes no secondary pollution, and is stable in treatment effect, environment-friendly and non-toxic.

The invention discloses an industrial sewage treatment agent, comprising polyacrylamide, silicon dioxide, peroxysulphate, sodium carbonate and compound microorganisms, wherein the compound microorganisms comprise bacillus licheniformis, pseudomonas aeruginosa, bacillus cereus, candida lipolytica, nitrifying bacteria and paracoccus denitrificans. By adopting the industrial sewage treatment agent disclosed by the invention, the removal rate of sewage suspension solids can be up to 99.8%, the biochemical oxygen demand (BOD) can be up to 98.7%, the chemical oxygen demand (COD) removal rate can be up to 98%, the industrial sewage treatment agent is less in sludge amount, free of secondary pollution, stable in treatment effect, environment-friendly, and non-toxic.

The invention relates to the technical field of biology and particularly relates to paracoccus denitrificans and an application thereof in treatment of livestock farm wastewater. The paracoccus denitrificans TDFQ-1 is collected at the China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO:M2017238. The bacterial strain has the advantages of being high in growth speed, high in environmental tolerance and good in growth in an organic culture medium. The paracoccus denitrificans has a heterotrophic nitrification capability and an aerobic denitrification capability, and is capable of reducing harmful substances, such as ammonia nitrogen and nitrate in water, especially removing the ammonia nitrogen in the livestock farm wastewater with relatively high organic matter content and improving the breeding environment. The bacterial strain is capable of efficiently degrading fluoroquinolone antibiotics when treating the wastewater, reducing the pollution of the antibiotics to soil and water sources and protecting the human health.

The invention relates to the technical field of microbes, and especially discloses a composite microbial preparation used for river treatment. The composite microbial preparation used for river treatment comprises a compound microorganism, and the compound microorganism is composed of the following raw materials in percent by weight: 1-2% of nitrosococcus, 5-8% of thiobacillus thiooxidans, 8-15% of paracoccus denitrificans, 20-30% of bacillus spp, 8-18% of rhodospira, 6-15% of lactobacillus spp, 10-15% of saccharomyces cerevisiae, and 20-30% of bacillus subtilis. After being poured into polluted water, the composite microbial preparation used for river treatment is capable of obviously improving the water body blackening smelliness problem, reducing water-quality ammonium nitrogen and total phosphorus content, decomposing hydrogen sulfide and other harmful substances and improving water body environment. The composite microbial preparation used for river treatment is capable of improving the absorption utilization efficiency of water plants on nutrition elements and obviously improving the water body transparency, and possesses the advantages of stabile microbial preparation, strong purification capability, simple preparation, low cost and applicability to river treatment.

Belonging to the field of microbial environmental protection, the invention provides a microbial agent for treatment of sewage and sludge. The microbial agent mainly includes Rhodospirillum rubrum, rhodopseudomonas palustris, bacillus subtilis, Streptomyces microflavus, streptococcus thermophilus, candida utilis, Streptomyces viridis, pseudomonas aeruginosa, virgibacillus halodenitrificans, and Paracoccus denitrificans 10 bacteria. Each bacterium is subjected to conventional slant culture, shaking culture and seed tank cultivation respectively, the bacteria in the seed tank are mixed and then put into sewage directly, symbiosis of flora is utilized to reach the purpose of treating sewage and reducing sludge generation. The microbial agent provided by the invention can be applied to large-scale production and direct adding, is low in cost, and can adapt to changeful sewage components. And the flora has good impact resistance to sewage.

The invention belongs to the technical field of biology and environment protection, and aims at providing paracoccus denitrificans and a method for disposing single-cell protein through high ammonia-nitrogen wastewater. The adopted technical scheme is as follows: the invention provides hydrogen-oxidizing bacteria, namely paracoccus denitrificans Y5, which is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms on May 12, 2017 (address: Institute of Microbiology of Chinese Academy of Sciences, No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, 100101), with preservation number CGMCC No. 14118. The paracoccus denitrificans Y5 provided by the invention can remove COD and ammonia nitrogen in high ammonia-nitrogen wastewater, fix production of single-cell protein (SCP) by CO2, and can remove stink of wastewater.

The invention discloses a livestock and poultry biodegradable pad. The livestock and poultry biodegradable pad is made from a carrier and a mixed bacteria agent in a mixing and smashing mode. The mixed bacteria agent is made from bacillus pumilus, bacillus subtilis, bacillus licheniformis, acinetobacter calcoaceticus, paracoccus denitrificans and dung ball alkali-producing bacteria in a mixing, fermenting and drying mode. The livestock and poultry biodegradable pad has the advantages that livestock and poultry dung and urine are made to achieve zero emission and be free of pollution and free of stink, meanwhile, the livestock and poultry biodegradable pad contains natural antibiotics, improves the immunity of livestock and poultry, and can keep constant-temperature and stable fermentation; the livestock and poultry biodegradable pad can save land resources, the livestock and poultry breeding construction cost, manpower and resources.

The invention relates to a biological agent for treatment of industrial polluted water. The biological agent comprises a composite microbial agent and a carrier, wherein the composite microbial agent is prepared from pseudomonas putida, candida utilis, bacillus amyloliquefaciens, clostridium papyrosolvens, nitrosomonas europaea, paracoccus denitrificans, nitrobacter sp. and paracoccus pantotrophus. The strains are reasonably matched, symbiotic and harmonious and is not mutually antagonistic, the biological agent has a good degradation effect on aniline compounds difficult to degrade after being fed into a wastewater treatment system and is suitable for treatment of the industrial polluted water, the water treatment capacity is increased, the quality of treated water is improved, operation expenses are reduced, and up-to-standard emission is realized.

The invention relates to a treatment method for dephosphorizing and denitrifying sewage. The method is characterized by carrying out treatment by combining a physical method and a biological agent, wherein the biological agent comprises the following raw materials in parts by weight: 5-7 parts of paracoccus denitrificans, 5-7 parts of saccharomyces cerevisiae, 4-5 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 3-4 parts of clostridium papyrosolvens, 3-4 parts of penicillium ochrochloron, 2-3 parts of pseudomonas and 1-2 parts of bacillus cereus. The biological agent contains various microorganisms with excellent capacity of degrading refractory pollutants, is reasonable in compatibility of the strains, has good degrading effects and has a broad application prospect.

The invention belongs to the technical field of environmental protection, and discloses a sewage treatment method for garden planning. The sewage treatment method comprises the following steps: performing treatment through a preliminary sedimentation tank; performing filtration; performing secondary precipitation; performing treatment by a biological reaction tank. Mixed bacterium liquid being prepared from actinomycetes, bacillus alcaligenes, bacillus pumilus, candida albicans, bacillus amyloliquefaciens, papyrosolvens and paracoccus denitrificans is added into the biological reaction tank, sewage treatment is performed, 10 grams of a microbiological preparation is added into each cubic meter of liquid each time, adding is performed once every day and continually performed for one week, and finally standing is performed for 3 days, so that liquid is drained for garden irrigation. The method disclosed by the invention is suitable for sewage treatment of gardens, and treated water can be used for greening of communities and gardens, sprinkling to roads and the like, so that circular economic utilization of water is realized.

The present invention relates to a vancomycin production wastewater treatment method, which comprises: (1) carrying out coagulation precipitation treatment on vancomycin production wastewater, and filtering to remove precipitate; (2) carrying out anaerobic biochemical treatment on the effluent obtained in the step (1), and pouring Pseudomonas stutzeri FSTB-5; and (3) carrying out aerobic biochemical treatment on the effluent obtained in the step (2), and pouring a COD removing denitrogenation bacterial agent, wherein the COD removing denitrogenation bacterial agent comprises at least one selected from Pseudomonas stutzeri FSTB-5, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3, at least one selected from Arthrobacter FDN-1 and Flavobacterium aquatile FDN-2, and at least one selected from Kocuria palustris FSDN-A and Staphylococcus cohnii FSDN-C. According to the present invention, the coagulation precipitation-anaerobic biochemical treatment-aerobic biochemical treatment combination treatment process is used, the specific COD removing bacterial agent is poured into the anaerobic unit, and the specific COD removing denitrogenation bacterial agent is poured into the aerobic unit, such that the efficient removal of the COD and the total nitrogen from wastewater is achieved, and the treatment method has characteristics of simple process, high treatment efficiency, low treatment cost, and the like.

The invention discloses a compound microbiological preparation. The compound microbiological preparation comprises microorganisms and bio-enzyme, wherein the microorganisms contain bacillus subtilis,bacillus licheniformis, nitrosomonas, nitrosospira brieusis, P.Aeruginosa, paracoccus denitrificans, lactobacillus plantarum, lactobacillus casei, candida utilis, saccharomyces cerevisiae, rhodopseudomonas palustris, rhodospirillum, aspergillus niger and enterococcus faecalis; and the bio-enzyme contains microbial enzyme and an aerobic microbial agent. The compound microbiological preparation disclosed by the invention does not contain chemical substances, and secondary pollution to an environment is avoided, and therefore, the compound microbiological preparation is an environmental-friendlymicrobial agent. The preparation process of the compound microbiological preparation has the advantages that the preparation process is simple, the production cost is low, and the compound microbiological preparation has wide prospects on restoration of eutrophicated water bodies.

The invention belongs to the technical field of environmental protection and microorganisms, and finds and provides a set of screened and optimized rapid sewage organic matter degradation composite bacterial according to growth, functions and safety of microorganisms during biodegradation based on imperfection of the existing sewage treatment technology, and continuous amplification and applications of the corresponding effective bacterial so as to provide organic matter degradation efficiency in sewage treatment. The composite bacterial comprises bacillus cereus, bacillus subtilis, paracoccus denitrificans, bacillus badius, acinetobacter calcoaceticus, and bnfillus licheniformis. The present invention provides a rapid sewage organic matter degradation method, wherein sewage filled with a carrier is added with the composite bacteria, organic waste in the sewage can be synergistically degraded by protease, lipase and other decomposition enzymes produced by the composite bacterial, and a reduction rate of the organic matters can be about 80%. The invention further provides a method capable of amplifying effective bacterial in the existing organic sewage or the organic sewage to be treated so as to achieve high sewage treatment efficiency.