Paracoccus denitrificans and method for disposing single-cell protein through high ammonia-nitrogen wastewater

A technology for denitrifying Paracoccus and single-cell protein is applied in the field of wastewater deodorization, which can solve the problems of waste of resources and secondary pollution, and achieve the effects of reducing the volatilization of ammonia, reducing the concentration of ammonia nitrogen, and realizing the effects of COD and ammonia nitrogen.

Active Publication Date: 2018-03-06
广州清源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a plant that can remove COD and ammonia nitrogen in high ammonia nitrogen wa...

Method used

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  • Paracoccus denitrificans and method for disposing single-cell protein through high ammonia-nitrogen wastewater
  • Paracoccus denitrificans and method for disposing single-cell protein through high ammonia-nitrogen wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the screening of hydrogen oxidizing bacteria

[0034] Take 10mL of water from a fish pond in Longquanyi, Chengdu, into an anaerobic bottle containing 150mL of inorganic salt medium, and fill with H 2 , O 2 and CO 2 The mixed gas (the molar ratio is 7:2:1, the text is the molar ratio), the pH is 7, 150r / min shaker culture under the condition of 30 ℃. Re-inflate every 24 hours, replace the medium every 48 hours, and transfer with a 2% inoculation amount after the gas utilization tends to be stable. Repeated transfer in this way for 40 days, after the gas utilization was stable, the bacteria solution was taken for gradient dilution (10 -2 -10 -7) were inoculated in the roller tube inorganic salt medium, and cultured on a shaker at 30°C for 48 hours after aeration. Use an elbow capillary to pick colonies and culture them in liquid inorganic medium, and then use the plate streak method to separate a single colony for more than three times until the colonie...

Embodiment 2

[0035] Embodiment 2: the identification of hydrogen oxidizing bacteria

[0036] The isolated and purified bacterial strain was observed under a scanning electron microscope (SEM), and the results were as follows: figure 1 Shown: the bacterium is short rod-shaped, 0.5-1.1 μm long, without flagella; Gram staining identified the bacterium as negative.

[0037] The entire genome of the pure strain was extracted using a bacterial whole genome rapid extraction kit, PCR amplification was performed by selecting bacterial 16SrRNA universal primers 27F and 1492R, and then sequenced and analyzed. The sequencing results were compared with BLAST in the NCBI database, and the strain was identified as Paracoccus denitrificans, named Paracoccus denitrificans Y5, which is the preserved strain Paracoccus denitrificans Y5CGMCC No.14118 of the present invention.

[0038] Physiological and biochemical identification of Paracoccus denitrificans Y5, using BiologGenⅢMicroPlate to carry out 94 kinds...

Embodiment 3

[0040] Example 3: Optimizing test on autotrophic growth conditions of Paracoccus denitrificans Y5

[0041] Method steps: first prepare inorganic salt culture medium and divide it into 250mL anaerobic bottles, seal with butyl rubber stopper, sterilize at 121°C for 20min, and use displacement exhaust method to distribute gas: use latex tube to connect H 2 Gas cylinder, and connect the needle at the other end of the latex tube, insert this needle into the anaerobic bottle through the rubber plug, and then insert the other needle into the anaerobic bottle through the rubber plug to exhaust, and then press the mixture ratio (H 2 :O 2 :CO 2 =7:2:1) Inject the corresponding amount of O with a syringe 2 and CO 2 . Then inoculate the seed inoculum of the bacterial strain of the present invention into an inflated anaerobic bottle, monitor the gas utilization with a barometer, and supplement the gas intermittently. Single factor test was used to investigate the effects of different ...

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Abstract

The invention belongs to the technical field of biology and environment protection, and aims at providing paracoccus denitrificans and a method for disposing single-cell protein through high ammonia-nitrogen wastewater. The adopted technical scheme is as follows: the invention provides hydrogen-oxidizing bacteria, namely paracoccus denitrificans Y5, which is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms on May 12, 2017 (address: Institute of Microbiology of Chinese Academy of Sciences, No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, 100101), with preservation number CGMCC No. 14118. The paracoccus denitrificans Y5 provided by the invention can remove COD and ammonia nitrogen in high ammonia-nitrogen wastewater, fix production of single-cell protein (SCP) by CO2, and can remove stink of wastewater.

Description

technical field [0001] The invention belongs to the technical field of biology and environmental protection, and in particular relates to a newly screened denitrifying paracoccus and the production of single-cell protein by using high-ammonia-nitrogen waste water by the strain, and also relates to a method for deodorizing waste water. Background technique [0002] Fertilizer, coking, petrochemical, pharmaceutical, food, waste landfill and other industries will produce a large amount of high-ammonia-nitrogen wastewater, and the discharge of a large amount of ammonia-nitrogen wastewater into the water body will not only cause eutrophication of the water body, cause the water body to be black and smelly, and increase the difficulty and cost of water treatment. It even has toxic effects on people and organisms. At present, the treatment process of ammonia nitrogen wastewater mainly includes various treatment processes such as biological method and physical and chemical method. Ho...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F103/20C02F103/32C02F101/16
CPCC02F3/02C02F3/34C02F2101/16C02F2103/20C02F2103/32C12N1/20C12N1/205C12R2001/01Y02W10/10
Inventor 张小松
Owner 广州清源生物科技有限公司
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