Strain of paracoccus versutus and method for treating byproduct single cell protein in high ammonia nitrogen wastewater treatment

A single-cell protein, paracoccus technology, applied in animal husbandry wastewater treatment, food industry wastewater treatment, microorganism-based methods, etc., can solve the problems of resource waste, secondary pollution, etc., to reduce the volatilization of ammonia, and reduce the concentration of ammonia nitrogen. , the effect of alleviating the greenhouse effect

Active Publication Date: 2018-02-02
广州清源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a plant that can remove COD and ammonia nitrogen in high ammonia nitrogen waste

Method used

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  • Strain of paracoccus versutus and method for treating byproduct single cell protein in high ammonia nitrogen wastewater treatment
  • Strain of paracoccus versutus and method for treating byproduct single cell protein in high ammonia nitrogen wastewater treatment
  • Strain of paracoccus versutus and method for treating byproduct single cell protein in high ammonia nitrogen wastewater treatment

Examples

Experimental program
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Effect test

Example Embodiment

[0033] Example 1: Screening of hydrogen oxidizing bacteria

[0034] Take 5 g of a soybean rhizosphere soil in Longquanyi, Chengdu, and put it into an anaerobic bottle containing 150 mL of inorganic salt medium, fill it with H 2 , O 2 and CO 2 The mixed gas (molar ratio is 7:2:1, the whole text is the molar ratio), the pH is 7, 30 ℃ under the condition of 150r / min shaker culture. The gas was re-inflated every 24 hours, the medium was replaced at 48 hours, and the inoculation volume of 2% was transferred after the gas utilization became stable. So repeatedly cultivated for 40 days, after the gas utilization is stable, take the bacterial liquid gradient dilution (10 -2 ~10 -7) were inoculated in a rolling tube of inorganic salt medium, and incubated at 30°C for 48h on a shaker after aeration. Pick a single colony with an elbow capillary and cultivate it in a liquid inorganic medium, and then separate a single colony by streaking on a plate for more than three times until the...

Example Embodiment

[0035] Example 2: Identification of hydrogen oxidizing bacteria

[0036] The isolated and purified strains were observed under a scanning electron microscope (SEM). figure 1 Shown: the bacteria is short rod-shaped, 0.6-1.1 μm long, without flagella; Gram staining identified the bacteria as negative.

[0037] Bacterial whole genome rapid extraction kit was used to extract the whole genome of pure strains, and PCR amplification was carried out by selecting bacterial 16SrRNA universal primers 27F and 1492R, followed by sequencing analysis. The sequencing results were compared by BLAST in the NCBI database, and it was identified that the strain had 99% homology with Paracoccus versutus, which was Paracoccus versutus and was named Paracoccus versutus D6, namely It is the preserved strain of the present invention Paracoccus versicolor D6CGMCC No.14119.

[0038] Physiological and biochemical identification of Paracoccus versicolor D6 was carried out, and 94 biochemical experiments ...

Example Embodiment

[0040] Example 3: Optimization test of autotrophic growth conditions of Paracoccus versicolor D6

[0041] Method steps: First, prepare the inorganic salt medium and distribute it in 250mL anaerobic bottles, seal with butyl rubber stoppers, sterilize at 121°C for 20min, and use the displacement exhaust method for gas distribution: connect H with a latex tube 2 Connect the needle to the other end of the latex tube, insert the needle into the anaerobic bottle through the rubber stopper, and then insert the other needle into the anaerobic bottle through the rubber stopper for exhausting, and then press the mixed gas ratio (H 2 :O 2 : CO 2 =7:2:1) Use a syringe to inject the corresponding amount of O 2 and CO 2 . Then, the seed inoculum of the strain of the present invention is inoculated into an inflated anaerobic bottle, the gas utilization is monitored by a barometer, and the gas is supplemented intermittently. A single factor test was used to investigate the effects of dif...

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Abstract

The invention relates to the technical field of biology and environmental protection, and aims to provide a strain of paracoccus versutus and a method for treating a byproduct single-cell protein in high ammonia nitrogen waste water treatment. The technical scheme is that the invention provides a strain of hydrogenated bacterium-Paracoccus versutus D6, preserved in the China General Microbiological Culture Collection Center on May 12, 2017 (Address: No.3, first courtyard Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip code:100101), preservation number: CGMCC No.14119. The invention aims to solve problems of resources waste and secondary pollution in existing high ammonia nitrogen waste water treatment technologies and paracoccus versutus D6 provided by the invention can remove COD and ammonia nitrogen from the high ammonia nitrogen waste water and fix CO2 single cell protein (SCP), and at the same time remove odor of the wastewater.

Description

technical field [0001] The invention belongs to the technical field of biology and environmental protection, and specifically relates to a newly screened strain of Paracoccus fibrilis, and the production of single-cell protein by using high-ammonia-nitrogen waste water by the strain, and also relates to a method for deodorizing waste water. Background technique [0002] Fertilizer, coking, petrochemical, pharmaceutical, food, waste landfill and other industries will produce a large amount of high-ammonia-nitrogen wastewater, and the discharge of a large amount of ammonia-nitrogen wastewater into the water body will not only cause eutrophication of the water body, cause the water body to be black and smelly, and increase the difficulty and cost of water treatment. It even has toxic effects on people and organisms. At present, the treatment processes of ammonia nitrogen wastewater mainly include various treatment processes such as biological method and physical and chemical met...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F103/20C02F103/34C02F103/32
CPCC02F3/34C02F2101/16C02F2103/20C02F2103/32C02F2103/343C12N1/20C12N1/205C12R2001/01
Inventor 张小松
Owner 广州清源生物科技有限公司
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