A strain of Paracoccus mutans and method for treating by-produced single-cell protein in high ammonia nitrogen wastewater
A paracoccus, high ammonia nitrogen technology, applied in animal husbandry wastewater treatment, food industry wastewater treatment, microorganism-based methods and other directions, can solve the problems of secondary pollution, resource waste, etc., to reduce the volatilization of ammonia, reduce the concentration of ammonia nitrogen, Mitigating the effect of the greenhouse effect
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Embodiment 1
[0033] Embodiment 1: the screening of hydrogen oxidizing bacteria
[0034] Take 5g of a soybean rhizosphere soil in Longquanyi, Chengdu, into an anaerobic bottle containing 150mL of inorganic salt medium, and fill with H 2 , O 2 and CO 2 The mixed gas (the molar ratio is 7:2:1, the text is the molar ratio), the pH is 7, 150r / min shaker culture under the condition of 30 ℃. Re-inflate every 24 hours, replace the medium every 48 hours, and transfer with 2% inoculation amount after the gas utilization tends to be stable. Repeatedly cultivated in this way for 40 days, and after the gas utilization was stabilized, the bacterial solution was taken for gradient dilution (10 -2 ~10 -7) were inoculated in the roller tube inorganic salt medium, and cultured on a shaker at 30°C for 48 hours after aeration. Use an elbow capillary to pick a single colony and culture it in a liquid inorganic medium, and then use the plate streak method to separate a single colony for more than three tim...
Embodiment 2
[0035] Embodiment 2: the identification of hydrogen oxidizing bacteria
[0036] The isolated and purified bacterial strains were observed under a scanning electron microscope (SEM), and the results are shown in the attached figure 1 Shown: the bacterium is short rod-shaped, 0.6-1.1 μm long, without flagella; the bacterium is negative by Gram staining.
[0037] The entire genome of the pure strain was extracted using a bacterial whole genome rapid extraction kit, PCR amplification was performed by selecting bacterial 16SrRNA universal primers 27F and 1492R, and then sequenced and analyzed. The sequencing results were compared with BLAST in the NCBI database, and it was identified that the strain was 99% homologous to Paracoccus versutus, and it was named Paracoccus versutus D6, namely The preserved bacterial strain of the present invention is Paracoccus varitans D6CGMCC No.14119.
[0038] Physiological and biochemical identification of Paracoccus variabilis D6, using BiologGe...
Embodiment 3
[0040] Embodiment 3: Optimizing test of autotrophic growth conditions of Paracoccus fibridis D6
[0041] Method steps: first prepare inorganic salt culture medium and divide it into 250mL anaerobic bottles, seal with butyl rubber stopper, sterilize at 121°C for 20min, and use displacement exhaust method to distribute gas: use latex tube to connect H 2 Gas cylinder, and connect the needle at the other end of the latex tube, insert this needle into the anaerobic bottle through the rubber plug, and then insert the other needle into the anaerobic bottle through the rubber plug to exhaust, and then press the mixture ratio (H 2 :O 2 :CO 2 =7:2:1) Inject the corresponding amount of O with a syringe 2 and CO 2 . Then inoculate the seed inoculum of the bacterial strain of the present invention into an inflated anaerobic bottle, monitor the gas utilization with a barometer, and supplement the gas intermittently. Single factor test was used to investigate the effects of different pH...
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