Cultural method for paracoccus denitrificans and application of same to purifying aquaculture water

A technology for denitrifying Paracoccus and a culturing method, which is applied in the field of aquaculture, can solve the problems of effect of environmental conditions, single action object, and cure the symptoms but not the root cause, and achieves the effect of reducing the density of algae, using a small amount, and reducing pollution.

Inactive Publication Date: 2013-02-06
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical improvers act quickly, have intuitive effects, and use small amounts, but they act on a single object, last for a short time, and are often ecologically unsafe, treating the symptoms but not the root cause; Mainly, safe and effective, but the onset time is slow, the dosage is large, and the effect is unstable due to environmental conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 The cultivation of a denitrifying paracoccus of the present invention and its application method in the purification of aquaculture water quality adopt the following process steps:

[0019] (1) Cultivation of denitrifying paracoccus:

[0020] Take a ring of denitrifying paracoccus from the slant of the test tube ( Paracoccus denitrificans , ACCC10489, provided by the China Agricultural Microorganism Culture Collection Management Center) bacterial lawn, inoculated into 50mL sterilized LB liquid medium, at 30 ℃, 100 r min -1 Incubate on a shaker for 24 hours. Then it was inserted into 5L of sterilized LB liquid medium, at 30 °C, 100 r min -1 Cultivate on a shaker for 24 hours, and prepare the strain seed solution for later use. OD of the bacterial solution at this time 600 The value is approximately equal to 1.0, and the number of viable bacteria is ≥10 9 CFU·mL -1 .

[0021]Add a total volume of 350 L of liquid fermentation medium into a 0.5-ton stain...

Embodiment 2

[0033] Embodiment 2: the cultivation of a kind of denitrification paracoccus of the present invention and the application method in the purification of aquaculture water quality adopt the following process steps:

[0034] (1) Cultivation of denitrifying paracoccus:

[0035] Take a ring of denitrifying paracoccus from the slant of the test tube ( Paracoccus denitrificans , ACCC10489, provided by the China Agricultural Microorganism Culture Collection Management Center) bacteria lawn, inoculated into 100mL sterilized LB liquid medium, at 35 ℃, 150 r min -1 Incubate on a shaker for 30 h. Then put it into 50L of sterilized LB liquid medium, at 35 ℃, 150 r min -1 Cultivate on a shaker for 30 hours, and prepare the strain seed solution for later use. OD of the bacterial solution at this time 600 The value is approximately equal to 1.0, and the number of viable bacteria is ≥10 9 CFU·mL -1 .

[0036] Add a liquid fermentation medium with a total volume of 350 L into a 0.5-ton s...

Embodiment 3

[0043] Embodiment 3: the cultivation of a kind of denitrifying paracoccus of the present invention and the application method in the purification of aquaculture water quality adopt the following process steps:

[0044] (1) Cultivation of denitrifying paracoccus:

[0045] Take a ring of denitrifying paracoccus from the slant of the test tube ( Paracoccus denitrificans , ACCC10489, provided by the China Agricultural Microorganism Culture Collection Management Center) bacterial lawn, inoculated into 50mL sterilized LB liquid medium, at 30 ℃, 100 r min -1 Incubate on a shaker for 24 hours. Then it was inserted into 5L of sterilized LB liquid medium, at 30 °C, 100 r min -1 Cultivate on a shaker for 24 hours, and prepare the strain seed solution for later use. OD of the bacterial solution at this time 600 The value is approximately equal to 1.0, and the number of viable bacteria is ≥10 9 CFU·mL -1 .

[0046] Add a total volume of 700L liquid fermentation medium into a 1.0-ton...

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Abstract

The invention relates to a cultural method for paracoccus denitrificans and application of the same to purifying aquaculture water, and belongs to the technical field of aquaculture. Various low-value materials such as molasses and waste liquid of monosodium glutamate and production waste which are harmless for aquaculture organisms and the paracoccus denitrificans are used as raw materials, the raw materials are reasonably compounded into liquid culture media, paracoccus denitrifican liquid with high activity and high concentration is produced, the liquid is directly fed into the aquaculture water, an ecological environment of the aquaculture water is repaired, and an excellent water environment beneficial to survival and growth is created for the aquaculture organisms. The culture method and the application have the advantages that the paracoccus denitrificans become effective fast after being used, an inorganic nitrogen removal form and the removal speed can be selected according to an oxygen dissolving condition of the water, contents of harmful substances such as ammonia nitrogen, nitrite and nitrate in the water are remarkably reduced, the quality of the water environment and a survival condition for the aquaculture organisms are improved, the yield of the aquaculture organisms is increased, and the quality of the aquaculture organisms is improved.

Description

technical field [0001] The invention relates to a method for cultivating denitrifying paracoccus and its application in the purification of aquaculture water, belonging to the technical field of aquaculture. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly, and the scale of aquaculture has continued to expand. In order to pursue high yield and high efficiency, people often put a large amount of bait and fertilizer rich in nitrogen and other nutrients into the aquaculture water, resulting in inorganic nitrogen (NH 4 + -N, NO 2 - -N, NO 3 - -N) excessive accumulation will affect the quality of water ecological environment. Nitrite nitrogen in inorganic nitrogen is a carcinogen, which can cause cancer of cells and tissues, and can oxidize ferrous hemoglobin (Fe 2+ ) to generate methemoglobin (Fe 3+ ), making it lose the ability to carry oxygen molecules, thereby affecting the transport of oxygen in aquatic animals, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 吴伟刘道玉王琼瞿建宏
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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