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Aerobic denitrifying Paracoccus denitrificans and application thereof

A denitrification paracoccus, nitrification denitrification technology, applied in the direction of aerobic and anaerobic process treatment, bacteria, etc., can solve the problem that the total nitrogen removal rate is less than 50%, the performance has not been reported, and further improve it to overcome the treatment Efficiency and economical applicability, efficient and economical denitrification, and stable biological activity

Active Publication Date: 2012-05-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bacteria can degrade nitrate nitrogen from 282.0mg / L to 149.2mg / L within 5 days, and the removal rate of total nitrogen is less than 50%.
Zhu Yueqi (Study on the classification and characteristics of an aerobic denitrifying bacterium, Bulletin of Microbiology, 2009, 04) isolated an aerobic denitrifying bacterium CY1, which was initially identified as Paracoccus pantotrophus. Both have denitrification ability under anaerobic and aerobic conditions, the initial concentration of nitrate nitrogen is 137.25mg / L, and the removal rate of nitrate nitrogen within 30h is 99.98% (anaerobic) and 60.16% (aerobic), but the bacteria The performance of heterotrophic nitrification to ammonia nitrogen has not been reported, and it is limited in the treatment of ammonia nitrogen-containing sewage. In addition, the denitrification effect under aerobic conditions needs to be further improved.

Method used

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  • Aerobic denitrifying Paracoccus denitrificans and application thereof
  • Aerobic denitrifying Paracoccus denitrificans and application thereof
  • Aerobic denitrifying Paracoccus denitrificans and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Examination of the aerobic denitrification performance of Paracoccus denitrificans DN-3.

[0033] Preparation simulated waste water I (sodium succinate 4.3g, KNO 3 2.0g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH7.0~7.5), the initial concentration of nitrate nitrogen is 277mg / L, and the mass ratio of carbon to nitrogen is 2.8. Treat for 26 hours under the conditions of dissolved oxygen (DO) 0.5mg / L, temperature 22°C, and pH 7.0. The removal rate of nitrate nitrogen and total nitrogen is greater than 95%, and the removal rate of COD is greater than 90%.

[0034] Prepare simulated wastewater II (methanol 5.0mL, KNO 3 2.0g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH7.0~7.5), the initial concentration of nitrate nitrogen is 277mg / L, and the mass ratio of carbon to nitrogen is 5.3. Treat for 29 hours under the conditions of DO 1.5mg / L, temperature 30°C, and pH 8.0. Nitra...

Embodiment 2

[0036] Heterotrophic nitrification-aerobic denitrification performance of Paracoccus denitrificans DN-3.

[0037] Preparation of simulated wastewater III (sodium succinate 4.3g, ammonium sulfate 1.25g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, water 1.0L, pH7.0~7.5), the initial concentration of ammonia nitrogen is 265mg / L, and the mass ratio of carbon to nitrogen is 2.9. Treat for 34 hours under the conditions of DO 0.5mg / L, temperature 25°C, and pH 7.5. The removal rate of ammonia nitrogen and total nitrogen is greater than 95%, and the removal rate of COD is greater than 90%.

[0038] Prepare simulated wastewater IV (methanol 5.0mL, ammonium sulfate 1.25g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, water 1.0L, pH7.0~7.5), the initial concentration of ammonia nitrogen is 265mg / L, and the mass ratio of carbon to nitrogen is 5.6. Cultivate for 36 hours under the conditions of DO 2.0 mg / L, temperature 35°C,...

Embodiment 3

[0040] Denitrification Paracoccus DN-3 is used in MSG wastewater with high concentration of COD.

[0041] MSG wastewater quality: ammonia nitrogen concentration 300mg / L-450mg / L, COD 700mg / L-850mg / L. First, use the nitrifying bacteria cultivated in this laboratory to convert ammonia nitrogen into nitrate nitrogen, and use the nitrification effluent as denitrification influent, then use DN-3 to carry out aerobic denitrification, and add methanol when the carbon source is insufficient, so that the mass ratio of carbon to nitrogen is 3.0. The initial nitrate nitrogen concentration was 280mg / L, and the COD was 720mg / L. After treatment for 32 hours under the conditions of DO 0.5mg / L, temperature 30°C, and pH 7.0-8.5, the removal rate of nitrate nitrogen and total nitrogen was greater than 90%, and the removal rate of COD was greater than 80%.

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Abstract

The invention relates to aerobic denitrifying paracoccus denitrificans and application thereof. A bacterial strain provided in the invention is paracoccus denitrificans DN-3CGMCC No. 3658; the Paracoccus denitrificans can carry out aerobic denitrification by using nitrate nitrogen under both aerobic and oxygen-limited conditions and can also carry out heterotrophic nitrification-aerobic denitrification by using ammonia-N, and a total nitrogen removal rate on above mentioned occasions is greater than 90%. The paracoccus denitrificans provided in the invention has stable hereditary features, isapplicable to treatment of a variety of nitrogen-containing waste water and produces a good nitrogen removal effect.

Description

technical field [0001] The invention relates to the field of environmental microorganisms. In particular, it relates to a paracoccus denitrificans used for biological denitrification and its application. The denitrifying paracoccus of the present invention has strong denitrification performance under aerobic conditions, and is especially suitable for denitrification in the synchronous nitrification and denitrification process of ammonia-containing wastewater. Background technique [0002] With the increasing nitrogen pollution in water bodies, denitrification has become the focus and hotspot in water treatment research. At present, it is generally believed that biological denitrification is one of the more economical and effective methods to remove nitrogen pollution from wastewater. Traditional biological denitrification includes two processes: aerobic nitrification and anaerobic denitrification. Nitrification refers to the oxidation of ammonia nitrogen to nitrite nitroge...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/30
Inventor 李志瑞高会杰黎元生许谦刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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