A strain denitrogen paracoccus and its culturing method and application

A technology for denitrifying paracoccus and its cultivation method, which is applied in the field of denitrifying paracoccus and its cultivation, and can solve problems such as pollution and the difficulty of effective treatment of "three wastes"

Active Publication Date: 2006-11-29
BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The pollution situation in my country is obviously different from that in other countries. The existing commercial biological agents are difficult to effectively control the "three wastes", and the serious pollution situation needs to be solved urgently.

Method used

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  • A strain denitrogen paracoccus and its culturing method and application
  • A strain denitrogen paracoccus and its culturing method and application
  • A strain denitrogen paracoccus and its culturing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and purification of Paracoccus denitrificans W12 CGMCC No.1673

[0031] Paracoccus denitrificans W12 is a G-bacterium isolated and purified from the activated sludge cultivated by the Department of Environmental Engineering of Tsinghua University using toxic and harmful industrial wastewater for long-term domestication. The specific enrichment, separation and purification process as follows:

[0032] Under the condition of selection pressure of 2000 mg / L pyridine, that is, using pyridine as the only carbon and nitrogen source, Paracoccus denitrificans was obtained from the activated sludge domesticated for a long time through enrichment, separation, screening, purification and other steps. ) W12 CGMCC No. 1673.

[0033] The specific separation process is as follows: Inoculate the collected samples into a shaker flask containing liquid BS pyridine selection medium (add 2000 mg / L pyridine as the only carbon source and nitrogen source in BS inorganic ...

Embodiment 2

[0034] Example 2, Pollutant Degradation Spectrum and Resistance Spectrum of Paracoccus denitrificans W12 CGMCC No.1673

[0035] 1) Pollutant degradation spectrum of Paracoccus denitrificans W12 CGMCC No.1673

[0036] The BS inorganic salt medium was used as the basic medium, and the organic pollutants shown in Table 1 were used as the only added energy, carbon source, and nitrogen source. It was cultured in a solid state at 30°C for one week, and the colony formation was observed. Condition. The test results of benzene, phenol, toluene, xylene, pyridine, cyanide, quinoline, imidazole and other compounds are shown in Table 1. The results showed that Paracoccus denitrificans (Paracoccus denitrificans) W12 CGMCC No.1673 had a broad degradation spectrum and could grow by using a variety of organic pollutants, especially for pyridine.

[0037] pyridine

Xylene

benzene

phenol

Toluene

quinoline

CN -

imidazole

concentration

1.96g / L...

Embodiment 3

[0044] Example 3, Biodegradation of Pollutants by Paracoccus denitrificans W12 CGMCC No.1673

[0045] 1) Biodegradation of pyridine-contaminated wastewater by Paracoccus denitrificans W12 CGMCC No.1673

[0046] Pick 2-ring denitrificans paracoccus (Paracoccus denitrificans) W12 CGMCC No.1673 bacteria from the slope and inoculate them in a 300ml Erlenmeyer flask containing 50ml LB liquid medium, culture at 35°C with shaking at 180rpm for 36h, centrifuge at 8000rpm for 10min to collect the bacteria, and then The bacteria solution was washed with sterile physiological saline, and then centrifuged, and this was repeated twice, and the centrifugation condition was also 8000 rpm for 10 min. Finally, the obtained bacterium was resuspended with BS culture solution added with pyridine, and inserted into a 300ml Erlenmeyer flask containing 50ml of waste water with pyridine. The concentration of pyridine was about 50mg / l, 100mg / l, 200mg / l, 400mg / l l, 900mg / l, 1400mg / l; 35°C, 150rpm, for...

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Abstract

The invention discloses a denitrogenation paracoccus strain and the cultivating method and the application. The strain is Paracoccus denitrificans W12 CGMCC No.1673. It has the strong degradation ability to organic pollutant, and could be used to take the biology treatment to the polluted water containing benzene, xylene, quinoline and cyanide and to the polluted soil. Especially, it has high degradation activity to benzene.

Description

technical field [0001] The invention relates to a strain of denitrifying paracoccus and its cultivation method and application. Background technique [0002] Inspired by the "self-purification" process of the earth, biological treatment has the advantages of low cost, light secondary pollution, and good environmental compatibility. Biological treatment technology has now become one of the preferred solutions in pollution control technology, and has produced good results. The activated sludge method, oxidation ditch method, etc. that can be used for sewage / wastewater treatment. The factors that determine the success, effectiveness, and applicability of biochemical treatment processes include not only process conditions and operation management, but also the role of functional microbial populations used in the degradation and transformation of pollutants. Therefore, the isolation and screening of special microorganisms, the isolation and extraction of functional genes, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34
Inventor 李毅王军温东辉乔琳沙圣德刘江江王喜萍夏勉
Owner BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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