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Treatment method and application of antibiotic mushroom dregs

A technology of antibiotic residues and treatment methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve problems such as incomplete degradation and inability to degrade drug-resistant antibiotics, so as to improve degradation activity and promote expansion reproductive effect

Active Publication Date: 2020-04-10
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a treatment method and application of antibiotic residues to solve the technical problems in the prior art that the antibiotic residues are resistant to antibiotics due to antibiotic residues and cannot be degraded or degraded incompletely

Method used

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  • Treatment method and application of antibiotic mushroom dregs

Examples

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Effect test

Embodiment 1

[0032] (1) 90g of tetracycline scum samples are placed in the reaction tank (moisture content 85%, dry basis organic components contain 90%, inorganic components 10%, cephalosporin C content 7050mg / kg dry scum, pH5.5), Under the conditions of room temperature 20°C and microwave power 600W, the microwave heating process takes about 200 seconds. After reaching the target temperature of 100°C, continue processing for 30 minutes in constant power mode;

[0033] (2) Inoculate the slant preservation of Phanerochaete chrysosporium (purchased with Beijing Kezhan Biotechnology Co., Ltd.) into potato dextrose solid medium, and place it at 35° C. to cultivate for 3-5 days until a large number of spores are formed; Add a certain amount of Tween80-NaCl solution to the solid medium, gently scrape off the spores with a triangular scraper, mix them into water, absorb the aqueous solution containing spores, filter to remove mycelia, count the filtrate on a hemocytometer and dilute to a spore co...

Embodiment 2

[0038] Same as Example 1, the difference is that the antibiotic residue is oxytetracycline residue.

Embodiment 3

[0040] The same as in Example 1, the difference is that the antibiotic residue prepared in step (1) is directly mixed with pig manure, and the Phanerochaete chrysosporium culture solution of the same quality is added, and after uniform mixing, aerobic composting fermentation is carried out.

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Abstract

The invention discloses a treatment method and application of antibiotic mushroom dregs. The method comprises the following steps: putting fresh antibiotic mushroom dregs into a reaction tank; carrying out uniform mixing under stirring, and then putting the formed mixture into a microwave reactor for microwave regulation and control; then uniformly mixing the mixture with a phanerochaete chrysosporium culture solution; carrying out filtering after standing for 1-2 days, collecting a solid part, and uniformly mixing the solid part with organic waste; and putting the formed mixture into a fermentation tank, and carrying out groove type aerobic composting fermentation. The treated antibiotic mushroom dregs can be used as a fertilizer due to high nitrogen content, can improve soil and does notpollute the environment. The method has good application prospects and can be applied to gardening, fruits and vegetables, farmlands, forests, urban landscaping lands and the like.

Description

technical field [0001] The invention belongs to the technical field of reutilization of antibiotic scum resources, and in particular relates to a treatment method and application of antibiotic scum. Background technique [0002] Antibiotic scum refers to the mycelium waste residue produced during the antibiotic fermentation production process, which belongs to industrial waste. The main components are mycelium, metabolites produced during the fermentation process, degradation products of the medium, residual organic solvents, and residual antibiotics. The antibiotic residues contained in the fungus residue will be enriched in the animal body, and enter the milk and eggs through lactation and egg production, and then cause toxic and side effects on humans, making pathogenic bacteria in the human body resistant to antibiotics. Therefore, how to effectively deal with antibiotic residues is not only the need to improve the economic benefits of enterprises, but also the need to s...

Claims

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Application Information

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IPC IPC(8): C05F15/00C12N1/14C12R1/02
CPCC05F3/00C12N1/14C05F5/008C05F7/00C05F9/00C05F11/00C05F11/08
Inventor 蔡辰戴晓虎刘惠玲华煜
Owner TONGJI UNIV
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