Efficient microorganism complex microbial inoculant

A technology of compound bacterial agent and streptomyces, which is applied in the direction of microorganisms, microorganism-based methods, and bio-organic part treatment, etc., can solve the problems of composting properties, high cellulose content, and long composting cycle, and achieve short fermentation cycle and faster fermentation. Compost is mature and easy to use

Active Publication Date: 2019-10-11
GUIZHOU NORMAL UNIVERSITY
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, these commercial bacteria agents still have problems such as long composting cycle (generally 2-6 months), low maturity and high cellulose content when added to compost
[0005] In addition, there are so many microorganisms that degrade compost, it is not easy to screen out practical bacterial agents. In particular, it is very challenging to obtain new bacterial agents that are more advantageous than commercial microbial agents. and may be affected by the nature of the compost
Therefore, there are many unpredictable situations in mixed bacterial agents, and it takes hard work and sometimes luck to obtain a truly practical microbial agent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient microorganism complex microbial inoculant
  • Efficient microorganism complex microbial inoculant
  • Efficient microorganism complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]The screening of embodiment 1 cellulose degrading bacteria

[0032] 1) Preliminary screening: select a total of 21 strains of commercial cellulose-degrading bacteria in the prior art. After these strains are activated, they are inoculated on the identification medium by spotting method. Each strain of bacteria is repeated three times, respectively at normal temperature (35-37 ℃) and high temperature (53-55 ℃) inverted culture for 3d-8d. After staining with 1mg / mL Congo red for 15min, decolorize with 1mol / L NaCl solution, based on the Dc value of the transparent circle (Dc=diameter of hydrolysis circle (D,cm) / diameter of colony (d,cm)), preliminarily judge the cellulose Cellulose-degrading ability of degrading strains.

[0033] 2) re-screening: 21 commercial bacterial strains for testing are activated respectively, and then some bacterial colonies are picked and inoculated into beef peptone liquid medium (beef extract 0.5g, peptone 1g, sodium chloride 0.5g, agar 2g, dist...

Embodiment 2

[0043] Antagonism test between bacteria in embodiment 2

[0044] The bacterial strains after activation in Table 2 were streaked on the PDA medium plate respectively, such as image 3 shown. Incubate at a constant temperature of 37°C for 7 days, and observe the growth status of the strains in pairs at the crossed lines. If the growth of the two strains at the intersection point is relatively weak or does not grow, it means that there is antagonism between the two strains; if the bacteria at the intersection point grow well, it means that there is no antagonism between the two strains and can be used for mixed culture. The test found that most of the strains had no obvious antagonistic effect, and they could be mixed cultured, see image 3 .

[0045] Some strains have certain antagonistic effects. For example, Rhizobium cellulosilyticum CGMCC 1.15995 and Streptomyces psammoticus CGMCC 4.1465 have high enzyme activity, but they have partial inhibitory effect on Bacillus, and ...

Embodiment 3

[0054] Embodiment 3 composite bacterial agent combination and optimization

[0055] In order to further select the optimal combination of bacterial agents, the application further selects and optimizes the combination of bacterial agents. The combination of compound bacterial agents is shown in the table below, and the corresponding bacterial strains are respectively inoculated in beef peptone medium, potato medium and Gaoshi No. I In the culture medium, the bacterial suspension was obtained by shaking culture. The bacterial strains were mixed in equal volumes to prepare composite bacterial agents, and the total volume of each combined bacterial agent was guaranteed to be consistent. The results of the enzyme activity verification were as follows.

[0056] Table 3 FPA and CMC of different combinations of bacterial agents

[0057]

[0058] The test shows that the enzyme activity of the above-mentioned compound bacterial agents is significantly higher than that of a single b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an efficient complex microbial inoculant for composting. The complex microbial inoculant is mainly prepared by mixing Hunan paenibacillus, bacillus subtilis, penicillium oxalicum, Penicillium rugulosum, Streptomyces erythrochromogenes and Streptomyces thermocoprophilus proportionally. According to the complex microbial inoculant, the strains are compatible reasonably, withno antagonism, harmony and co-existence are achieved, after the inoculant is mixed with compost raw materials, stack retting is performed for manufacturing fertilizer, composting can be remarkably accelerated, the fermentation period is short, the composting efficiency is improved, use is convenient, and the inoculant is suitable for industrial production.

Description

technical field [0001] The present application relates to the field of compost fermentation, in particular to a microbial agent for efficiently degrading compost. [0002] technical background [0003] With the rapid development of the livestock and poultry breeding industry, while producing huge economic and social benefits, a large amount of livestock and poultry manure is also produced, resulting in a sharp increase in environmental pressure. How to properly and effectively treat and use manure has become an important problem that needs to be solved urgently . High-temperature aerobic composting is a more economical and effective treatment method for livestock and poultry manure. It can not only solve the problem of livestock and poultry manure pollution, but also convert livestock and poultry manure composting into bio-organic fertilizer, which is one of the most effective ways for its resource utilization , is also in line with the best treatment method of our country's...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F11/08C12R1/125C12R1/80C12R1/465C12R1/01
CPCC12N1/20C12N1/14C05F11/08C05F17/20Y02W30/40
Inventor 翁庆北周桂雄吴庆珊
Owner GUIZHOU NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap