14 results about "Biomass degradation" patented technology

The utility model discloses a system for preparing renewable natural gas by utilizing biomass, which comprises a biomass degradation system, a biomass combustion system, a biomass gasification system and a wind and light energy storage electrolysis system, the biomass degradation system comprises an anaerobic fermentation device, a purification device and a biogas storage tank I which are communicated in sequence, the biomass combustion system comprises a biomass boiler, a carbon dioxide trapping device, a methanation device I and a synthetic natural gas storage tank which are sequentially communicated, and the biomass gasification system comprises a gasification furnace, a methanation device II and a biogas storage tank II which are sequentially communicated. The wind and light energy storage electrolysis system comprises a wind power and photovoltaic power generation system and an electrolytic cell which are sequentially communicated. The biomass recycling system has the advantages that recycling treatment of different types of biomass is realized, and meanwhile, efficient development and utilization of biomass energy are also realized.

This invention relates to the field of biomass degradation technology, specifically to a ruthenium-based catalyst Ru@RuO2 supported by zirconium phosphate, its preparation method, and its application in catalyzing the hydrogenolysis of lignin. This invention is based on the in-situ reduction of elemental ruthenium to needle-like ruthenium oxide supported by amorphous zirconium phosphate, constructing a RuO2 / zirconium phosphate composite nanomaterial. This material can efficiently catalyze the hydrogenolysis of alkali-degraded lignin and model compounds such as α-O-4 and 4-O-5. Furthermore, the ruthenium nanoparticles generated from the in-situ reduction on ruthenium oxide exhibit high stability and can be reused multiple times.

This invention discloses a method for extracting lignin with high phenolic hydroxyl content, belonging to the fields of biomass refining and green chemistry. The method involves pretreating plant fiber raw materials with bio-enzymes to obtain a solid residue; then, the solid residue is extracted and purified using a catalytic eutectic solvent to obtain the lignin with high phenolic hydroxyl content. A two-step strategy of "enzyme pretreatment exposure + catalytic DES directional bond breaking" is employed to extract lignin with high phenolic hydroxyl content from plant fibers. Enzyme pretreatment effectively breaks down the biomass degradation barrier, removing most of the carbohydrates and fully exposing the lignin. This not only results in a lignin yield of over 90% for subsequent DES extraction, but more importantly, it creates an optimal interaction interface for the DES catalyst to selectively break the β-O-4 ether bonds of lignin molecules, thereby generating a large number of phenolic hydroxyl groups in situ, significantly increasing the lignin content to far exceed that of lignin extracted by traditional methods and conventional DES, and producing a product with excellent reactivity.

The application discloses a xylanase mutant with simultaneously improved thermal stability and pH tolerance and application thereof, and belongs to the field of genetic engineering and protein engineering. The xylanase AbXyL10A from Agaricus bisporus var burnettii is used as a parent to obtain two xylanase mutants T234N and R268L after mutation of two amino acid sites Thr234 and Arg268. Compared with the wild-type xylanase, the thermal stability and pH tolerance of the two xylanase mutants are obviously improved. In terms of specific activity, when beech xylan is used as a substrate, the specific activity of the two xylanase mutants is obviously improved compared with the wild-type xylanase. The application provides a theoretical reference for improvement of xylanase, and the obtained xylanase mutant with simultaneously improved thermal stability and pH tolerance has great application prospect in animal feed and biomass degradation sugar.

The invention discloses difunctional xylanase as well as a preparation method and application thereof. According to the bifunctional xylanase, a C-terminal Dockeren structural domain is knocked out on the basis of wild type xylanase Xyn1G3 as shown in an amino acid sequence SEQ ID NO. 1, and the amino acid sequence of the bifunctional xylanase comprises a sequence as shown in SEQ ID NO. 3. A novel bifunctional enzyme with a simplified structure and optimized functions is successfully constructed by directional knockout of a Dockeren structural domain through a genetic engineering means, the bifunctional xylanase not only retains the original activity of xylanase, but also shows remarkable activity of cellulose hydrolase, and the application potential of the bifunctional xylanase in biomass degradation is expanded.

This invention belongs to the field of genetic engineering enzyme preparation technology, and particularly relates to a high-expression xylanase, gene editing, recombinant engineered bacteria, and their applications. This invention involves targeted amino acid site mutations in the wild-type xylanase Xyn-WT to obtain five mutant xylanases. Compared to the wild-type xylanase, the enzyme activities of all five mutants are significantly increased. Among them, the Xyn-4 mutant exhibits the best enzyme activity, reaching 245.17 U / mL, an increase of 90.60% compared to the wild type. Codon optimization further increased the expression level by 20.74%, demonstrating both high expression and excellent catalytic activity. The xylanase of this invention has high yield and high enzyme activity, making it suitable for industrial production scenarios such as feed, food, pulp and paper making, biomass degradation, and textiles, and possessing extremely high industrial application value.

The present invention discloses a method and application for directly utilizing lignocellulose to generate electricity, and relates to the field of microbial fuel cells. The present invention can solve the problem of ineffective utilization of waste biomass, especially the problem that lignocellulose cannot be directly utilized in MFCs. The specific method of the present invention comprises pretreating a lignocellulose raw material to obtain a pretreated substrate; activating Acetovibrio thermocellum to obtain a seed liquid; injecting the seed liquid into a dual-chamber microbial fuel cell using the pretreated substrate as an electron donor, and outputting electrical energy from the electrodes. The present invention achieves the coupling of waste biomass degradation and electricity production, has low production costs, high electricity generation efficiency, avoids secondary environmental pollution, and has high economic value and broad application prospects in the energy conversion of lignocellulose.