4 results about "Biomass degradation" patented technology

This invention relates to the field of biomass degradation technology, specifically to a ruthenium-based catalyst Ru@RuO2 supported by zirconium phosphate, its preparation method, and its application in catalyzing the hydrogenolysis of lignin. This invention is based on the in-situ reduction of elemental ruthenium to needle-like ruthenium oxide supported by amorphous zirconium phosphate, constructing a RuO2 / zirconium phosphate composite nanomaterial. This material can efficiently catalyze the hydrogenolysis of alkali-degraded lignin and model compounds such as α-O-4 and 4-O-5. Furthermore, the ruthenium nanoparticles generated from the in-situ reduction on ruthenium oxide exhibit high stability and can be reused multiple times.

This invention discloses a method for extracting lignin with high phenolic hydroxyl content, belonging to the fields of biomass refining and green chemistry. The method involves pretreating plant fiber raw materials with bio-enzymes to obtain a solid residue; then, the solid residue is extracted and purified using a catalytic eutectic solvent to obtain the lignin with high phenolic hydroxyl content. A two-step strategy of "enzyme pretreatment exposure + catalytic DES directional bond breaking" is employed to extract lignin with high phenolic hydroxyl content from plant fibers. Enzyme pretreatment effectively breaks down the biomass degradation barrier, removing most of the carbohydrates and fully exposing the lignin. This not only results in a lignin yield of over 90% for subsequent DES extraction, but more importantly, it creates an optimal interaction interface for the DES catalyst to selectively break the β-O-4 ether bonds of lignin molecules, thereby generating a large number of phenolic hydroxyl groups in situ, significantly increasing the lignin content to far exceed that of lignin extracted by traditional methods and conventional DES, and producing a product with excellent reactivity.

This invention belongs to the field of genetic engineering enzyme preparation technology, and particularly relates to a high-expression xylanase, gene editing, recombinant engineered bacteria, and their applications. This invention involves targeted amino acid site mutations in the wild-type xylanase Xyn-WT to obtain five mutant xylanases. Compared to the wild-type xylanase, the enzyme activities of all five mutants are significantly increased. Among them, the Xyn-4 mutant exhibits the best enzyme activity, reaching 245.17 U / mL, an increase of 90.60% compared to the wild type. Codon optimization further increased the expression level by 20.74%, demonstrating both high expression and excellent catalytic activity. The xylanase of this invention has high yield and high enzyme activity, making it suitable for industrial production scenarios such as feed, food, pulp and paper making, biomass degradation, and textiles, and possessing extremely high industrial application value.

The application discloses a kind of super double-tube spraying method and its application in biomass degradation, fungus growth, wrap deposition and the like.The super double-tube spraying method includes using high-pressure spray gun, and spraying mist drop after super double-tube, namely super double-tube spraying, wherein the super double-tube is PMMA polymer tube with super double-tube coating on inner wall, and the raw material for preparing the super double-tube coating includes TiO2 nanoparticle and hydrophobic epoxy resin.The application introduces super double-tube spraying technology, and by combining contact charging (CE) and induced charging (IE) effect, a kind of super double-tube spraying (SAP T-spraying) method is developed, which not only simplifies and optimizes the design of spraying, but also significantly improves the charge mass ratio (CMR) of mist drop, to realize efficient deposition on super-hydrophobic blade surface.