13 results about "Biomass degradation" patented technology

The utility model discloses a system for preparing renewable natural gas by utilizing biomass, which comprises a biomass degradation system, a biomass combustion system, a biomass gasification system and a wind and light energy storage electrolysis system, the biomass degradation system comprises an anaerobic fermentation device, a purification device and a biogas storage tank I which are communicated in sequence, the biomass combustion system comprises a biomass boiler, a carbon dioxide trapping device, a methanation device I and a synthetic natural gas storage tank which are sequentially communicated, and the biomass gasification system comprises a gasification furnace, a methanation device II and a biogas storage tank II which are sequentially communicated. The wind and light energy storage electrolysis system comprises a wind power and photovoltaic power generation system and an electrolytic cell which are sequentially communicated. The biomass recycling system has the advantages that recycling treatment of different types of biomass is realized, and meanwhile, efficient development and utilization of biomass energy are also realized.

This invention relates to the field of biomass degradation technology, specifically to a ruthenium-based catalyst Ru@RuO2 supported by zirconium phosphate, its preparation method, and its application in catalyzing the hydrogenolysis of lignin. This invention is based on the in-situ reduction of elemental ruthenium to needle-like ruthenium oxide supported by amorphous zirconium phosphate, constructing a RuO2 / zirconium phosphate composite nanomaterial. This material can efficiently catalyze the hydrogenolysis of alkali-degraded lignin and model compounds such as α-O-4 and 4-O-5. Furthermore, the ruthenium nanoparticles generated from the in-situ reduction on ruthenium oxide exhibit high stability and can be reused multiple times.

This invention discloses a method for extracting lignin with high phenolic hydroxyl content, belonging to the fields of biomass refining and green chemistry. The method involves pretreating plant fiber raw materials with bio-enzymes to obtain a solid residue; then, the solid residue is extracted and purified using a catalytic eutectic solvent to obtain the lignin with high phenolic hydroxyl content. A two-step strategy of "enzyme pretreatment exposure + catalytic DES directional bond breaking" is employed to extract lignin with high phenolic hydroxyl content from plant fibers. Enzyme pretreatment effectively breaks down the biomass degradation barrier, removing most of the carbohydrates and fully exposing the lignin. This not only results in a lignin yield of over 90% for subsequent DES extraction, but more importantly, it creates an optimal interaction interface for the DES catalyst to selectively break the β-O-4 ether bonds of lignin molecules, thereby generating a large number of phenolic hydroxyl groups in situ, significantly increasing the lignin content to far exceed that of lignin extracted by traditional methods and conventional DES, and producing a product with excellent reactivity.

The application discloses a xylanase mutant with simultaneously improved thermal stability and pH tolerance and application thereof, and belongs to the field of genetic engineering and protein engineering. The xylanase AbXyL10A from Agaricus bisporus var burnettii is used as a parent to obtain two xylanase mutants T234N and R268L after mutation of two amino acid sites Thr234 and Arg268. Compared with the wild-type xylanase, the thermal stability and pH tolerance of the two xylanase mutants are obviously improved. In terms of specific activity, when beech xylan is used as a substrate, the specific activity of the two xylanase mutants is obviously improved compared with the wild-type xylanase. The application provides a theoretical reference for improvement of xylanase, and the obtained xylanase mutant with simultaneously improved thermal stability and pH tolerance has great application prospect in animal feed and biomass degradation sugar.

The invention discloses difunctional xylanase as well as a preparation method and application thereof. According to the bifunctional xylanase, a C-terminal Dockeren structural domain is knocked out on the basis of wild type xylanase Xyn1G3 as shown in an amino acid sequence SEQ ID NO. 1, and the amino acid sequence of the bifunctional xylanase comprises a sequence as shown in SEQ ID NO. 3. A novel bifunctional enzyme with a simplified structure and optimized functions is successfully constructed by directional knockout of a Dockeren structural domain through a genetic engineering means, the bifunctional xylanase not only retains the original activity of xylanase, but also shows remarkable activity of cellulose hydrolase, and the application potential of the bifunctional xylanase in biomass degradation is expanded.

This invention belongs to the field of genetic engineering enzyme preparation technology, and particularly relates to a high-expression xylanase, gene editing, recombinant engineered bacteria, and their applications. This invention involves targeted amino acid site mutations in the wild-type xylanase Xyn-WT to obtain five mutant xylanases. Compared to the wild-type xylanase, the enzyme activities of all five mutants are significantly increased. Among them, the Xyn-4 mutant exhibits the best enzyme activity, reaching 245.17 U / mL, an increase of 90.60% compared to the wild type. Codon optimization further increased the expression level by 20.74%, demonstrating both high expression and excellent catalytic activity. The xylanase of this invention has high yield and high enzyme activity, making it suitable for industrial production scenarios such as feed, food, pulp and paper making, biomass degradation, and textiles, and possessing extremely high industrial application value.

The present invention discloses a method and application for directly utilizing lignocellulose to generate electricity, and relates to the field of microbial fuel cells. The present invention can solve the problem of ineffective utilization of waste biomass, especially the problem that lignocellulose cannot be directly utilized in MFCs. The specific method of the present invention comprises pretreating a lignocellulose raw material to obtain a pretreated substrate; activating Acetovibrio thermocellum to obtain a seed liquid; injecting the seed liquid into a dual-chamber microbial fuel cell using the pretreated substrate as an electron donor, and outputting electrical energy from the electrodes. The present invention achieves the coupling of waste biomass degradation and electricity production, has low production costs, high electricity generation efficiency, avoids secondary environmental pollution, and has high economic value and broad application prospects in the energy conversion of lignocellulose.

The present invention discloses a thermostable xylanase mutant and its preparation method and application, and relates to the field of genetic engineering and genetic engineering technology. Hortaea werneckii Using the 10th Family xylanase HwXyl10A as the parent, point mutagenesis was used to screen for mutants N318W and D372L. Both mutants exhibited significantly improved thermostability and catalytic activity. The present invention identifies two key amino acid residues that influence the thermostability of Family 10 xylanases, providing important guidance for improving the thermostability of Family 10 xylanases. It also provides a basis for the industrialized production of xylanases and their application in feed preparation and biomass degradation of xylan to produce sugars.

The invention belongs to the technical field of microbial metabolism engineering, and particularly relates to a lignin degrading bacterium, a separation method, a microbial agent and application of the lignin degrading bacterium in promoting biomass degradation. The strain is named as Trabulsiella odontobacteria L1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35692. The invention further discloses a preparation method of the strain. The invention provides a lignin degrading bacterium L1 which can degrade lignin under both an aerobic condition and an anaerobic condition, so that the degradation and conversion of biomass are promoted, and the yield is increased. Therefore, compared with the prior art, the lignin degrading bacterium L1 disclosed by the invention is unique in function, low in oxygen demand and beneficial to lignin degradation demands in different scenes.

The xylanase mutant and its application are obtained by mutation of Ser21 and Thr324 sites of the parent HwXyl10A. The xylanase mutant provided by the present invention has greatly improved thermal stability. The half-life of mutants S21Y and T324V at 75°C is ( t 1 / 2 ) were 88 min and 34 min, which were 14.7 times and 5.7 times that of the wild type (6 min), respectively; the mutants S21Y and T324V T 50 The optimal pH and temperature were 80.0°C and 76.5°C, respectively, 12.0°C and 8.5°C higher than the wild-type (68.0°C). The optimal pH and temperature were essentially the same as those of the wild-type, fully meeting the requirements for feed production and biomass degradation. This high-specific-activity thermostable xylanase mutant has broad application prospects in feed additives and biomass degradation for sugar production.

The application discloses a high-specific-activity endo-beta-1,4-glucanase EGH1, a preparation method and application thereof. The amino acid sequence of the EGH1 is shown as SEQ ID NO. 4. The specific enzyme activity of the enzyme is as high as 101.54 U / mg under the condition of pH=5.5 and 50 DEG C, and is significantly higher than that of commercial cellulase. Meanwhile, the enzyme activity of the EGH1 can be activated by Fe 2+ , and the enzyme activity is increased by about 60%. The enzyme can efficiently degrade natural lignocellulose without pretreatment, and the glucose release amount of the enzyme to the untreated bagasse is superior to that of the commercial enzyme under the same enzyme activity load, thus breaking the technical bottleneck that the existing cellulase seriously depends on a pretreatment process. In addition, the enzyme has the characteristics of good thermal stability, wide pH adaptation range and excellent metal ion tolerance, and can be widely applied to the biomass degradation process in the industries of biofuel, feed, textile and papermaking, and effectively reduces the production cost.

The application belongs to the field of environment and material technology, and discloses a method for optimizing pore channels of magnesium oxide modified biochar, which increases micropore volume in biochar through biomass degradation pretreatment, increases macropore volume in biochar through magnesium nitrate microwave foaming, increases mesopore volume in biochar through magnesium nitrate in-situ etching pyrolysis, realizes micropore, mesopore and macropore structure regulation in porous magnesium modified biochar through the synergistic effect of biomass degradation, in-situ etching and microwave foaming, and realizes hierarchical pore channel optimization. The application realizes the construction of porous structure magnesium modified biochar through the combined action of alkali solution, magnesium nitrate and magnesium acetate, wherein the magnesium nitrate microwave foaming and pyrolysis etching can respectively improve the macropore and mesopore structure of the material, and play a key role in the improvement of the pore structure and adsorption performance of the material. The method has the advantages of simplicity, easy industrialization, high control precision, excellent performance and the like.

The application discloses a kind of super double-tube spraying method and its application in biomass degradation, fungus growth, wrap deposition and the like.The super double-tube spraying method includes using high-pressure spray gun, and spraying mist drop after super double-tube, namely super double-tube spraying, wherein the super double-tube is PMMA polymer tube with super double-tube coating on inner wall, and the raw material for preparing the super double-tube coating includes TiO2 nanoparticle and hydrophobic epoxy resin.The application introduces super double-tube spraying technology, and by combining contact charging (CE) and induced charging (IE) effect, a kind of super double-tube spraying (SAP T-spraying) method is developed, which not only simplifies and optimizes the design of spraying, but also significantly improves the charge mass ratio (CMR) of mist drop, to realize efficient deposition on super-hydrophobic blade surface.