24 results about "Biomass degradation" patented technology

The invention discloses bacillus subtilis capable of producing cellulase at high yield and application of the bacillus subtilis, and belongs to the technical field of biology, the bacillus subtilis is classified and named as bacillus subtilis KC7-2, the bacillus subtilis KC7-2 is preserved in China Center for Type Culture Collection on July 15, 2024, and the preservation number is CCTCC NO: M20241570. The bacillus subtilis provided by the invention shows excellent performance in the aspect of secretion of cellulase and hemicellulase, especially reaches an enzymatic activity peak in the early growth stage (24h) of a strain, and can maintain a certain level of enzymatic activity for a long time later. Due to the characteristics, the strain has important application value in the fields of forage feed improvement, biomass degradation, environmental protection and the like.

The invention discloses a xylanase mutant with synchronously improved thermal stability and pH tolerance and application of the xylanase mutant, and belongs to the field of gene engineering and protein engineering. According to the invention, xylanase AbXyL10A from Agaricus bisporus var burnettii is taken as a female parent, and two amino acid sites of Thr234 and Arg268 are subjected to mutation, so that two types of xylanase mutants T234N and R268L are obtained. Compared with wild type xylanase, the thermal stability and pH tolerance of the two xylanase mutants are obviously improved. In the aspect of specific activity, when the beech xylan is used as a substrate, the specific activity of the two xylanase mutants is obviously improved compared with that of wild type xylanase. The invention provides a theoretical reference for the improvement of xylanase, and the obtained xylanase mutant with synchronously improved thermal stability and pH tolerance has a huge application prospect in the aspects of animal feed and biomass degradation sugar production.

The invention belongs to the technical field of environment and materials, and discloses a method for optimizing pore channels of magnesium oxide modified biochar, which comprises the following steps: increasing the volume of micropores in biochar through biomass degradation pretreatment; the volume of macropores in the biochar is increased through a magnesium nitrate microwave foaming part; the mesoporous volume of the biochar is increased through in-situ etching pyrolysis of magnesium nitrate; through the synergistic effect of biomass degradation, in-situ etching and microwave foaming, pore structure regulation and control of micropores, mesopores and macropores in the porous magnesium modified biochar can be achieved, and graded pore channel optimization is achieved. According to the preparation method, the construction of the magnesium modified charcoal with the porous structure is realized through the combined action of the aqueous alkali, magnesium nitrate and magnesium acetate, the macroporous structure and the mesoporous structure of the material can be respectively improved through microwave foaming and pyrolysis etching of magnesium nitrate, and a key role is played in improving the pore structure and the adsorption performance of the material. The method has the advantages of being simple, easy to industrialize, high in control precision, excellent in performance and the like.

The invention provides a high-activity pectin methylesterase mutant as well as preparation and application thereof. The amino acid sequence of the pectin methylesterase mutant PME-DS8 is as shown in SEQ ID NO: 2. According to the invention, a pectin methylesterase sequence is redesigned by using a large language model ESM-1v, and a mutant PME-DS8 with significantly improved enzymatic activity is screened in combination with multi-sequence alignment (MSA) analysis. Compared with wild pectin methylesterase, the enzymatic activity of the PME-DS8 is improved by about 3.2 times, and the PME-DS8 has higher industrial application value. The PME-DS8 mutant has a wide application prospect in the fields of food processing, spinning, papermaking and the like, can participate in biomass degradation, improves the conversion efficiency of raw materials, and promotes the production of biological energy sources.

The invention discloses a method for preparing xylobiose and xylotriose from agricultural and forestry waste bamboo sawdust, and belongs to the technical field of agricultural and forestry biomass degradation and conversion. The method comprises the following steps: firstly, adjusting the pH value of a lactic acid solution, then mixing the raw material bamboo sawdust with the lactic acid solution, hydrolyzing, and filtering to obtain a treatment solution containing xylooligosaccharide and filter residues; and then mixing the treating fluid with xylanase to carry out enzymolysis reaction, and further decomposing xylan and xylooligosaccharide with high polymerization degree in the aqueous solution into xylobiose and xylotriose. The xylobiose and the xylotriose are obtained by adjusting the pH value of the lactic acid solution in combination with an endo-xylanase hydrolysis method, so that the xylooligosaccharide with higher biological activity is prepared, and the xylooligosaccharide can be used as a functional feed additive and can also be used as a functional food additive through subsequent treatment.

The utility model discloses a system for preparing renewable natural gas by utilizing biomass, which comprises a biomass degradation system, a biomass combustion system, a biomass gasification system and a wind and light energy storage electrolysis system, the biomass degradation system comprises an anaerobic fermentation device, a purification device and a biogas storage tank I which are communicated in sequence, the biomass combustion system comprises a biomass boiler, a carbon dioxide trapping device, a methanation device I and a synthetic natural gas storage tank which are sequentially communicated, and the biomass gasification system comprises a gasification furnace, a methanation device II and a biogas storage tank II which are sequentially communicated. The wind and light energy storage electrolysis system comprises a wind power and photovoltaic power generation system and an electrolytic cell which are sequentially communicated. The biomass recycling system has the advantages that recycling treatment of different types of biomass is realized, and meanwhile, efficient development and utilization of biomass energy are also realized.

This invention relates to the field of biomass degradation technology, specifically to a ruthenium-based catalyst Ru@RuO2 supported by zirconium phosphate, its preparation method, and its application in catalyzing the hydrogenolysis of lignin. This invention is based on the in-situ reduction of elemental ruthenium to needle-like ruthenium oxide supported by amorphous zirconium phosphate, constructing a RuO2 / zirconium phosphate composite nanomaterial. This material can efficiently catalyze the hydrogenolysis of alkali-degraded lignin and model compounds such as α-O-4 and 4-O-5. Furthermore, the ruthenium nanoparticles generated from the in-situ reduction on ruthenium oxide exhibit high stability and can be reused multiple times.

The present invention discloses a salt-tolerant β-glucosidase and application, and relates to the field of biotechnology. The amino acid sequence of the β-glucosidase is shown in SEQ ID NO: 1. The present invention clones a novel β-glucosidase functional gene b0-bg40 belonging to the GH1 family from Karamay saline-alkali land using metagenomic technology, and realizes heterologous expression in Escherichia coli. The enzymatic properties of b0-bg40 are then characterized, and it is found that b0-bg40 is an alkali-resistant, thermally stable, and pH-wide β-glucosidase. In addition, b0-bg40 is tolerant to glucose and has a high affinity for cellobiose. These findings indicate that it has potential utilization value in the degradation of cellulose biomass and production in strong alkaline environments.

This invention discloses a method for extracting lignin with high phenolic hydroxyl content, belonging to the fields of biomass refining and green chemistry. The method involves pretreating plant fiber raw materials with bio-enzymes to obtain a solid residue; then, the solid residue is extracted and purified using a catalytic eutectic solvent to obtain the lignin with high phenolic hydroxyl content. A two-step strategy of "enzyme pretreatment exposure + catalytic DES directional bond breaking" is employed to extract lignin with high phenolic hydroxyl content from plant fibers. Enzyme pretreatment effectively breaks down the biomass degradation barrier, removing most of the carbohydrates and fully exposing the lignin. This not only results in a lignin yield of over 90% for subsequent DES extraction, but more importantly, it creates an optimal interaction interface for the DES catalyst to selectively break the β-O-4 ether bonds of lignin molecules, thereby generating a large number of phenolic hydroxyl groups in situ, significantly increasing the lignin content to far exceed that of lignin extracted by traditional methods and conventional DES, and producing a product with excellent reactivity.

The application discloses a xylanase mutant with simultaneously improved thermal stability and pH tolerance and application thereof, and belongs to the field of genetic engineering and protein engineering. The xylanase AbXyL10A from Agaricus bisporus var burnettii is used as a parent to obtain two xylanase mutants T234N and R268L after mutation of two amino acid sites Thr234 and Arg268. Compared with the wild-type xylanase, the thermal stability and pH tolerance of the two xylanase mutants are obviously improved. In terms of specific activity, when beech xylan is used as a substrate, the specific activity of the two xylanase mutants is obviously improved compared with the wild-type xylanase. The application provides a theoretical reference for improvement of xylanase, and the obtained xylanase mutant with simultaneously improved thermal stability and pH tolerance has great application prospect in animal feed and biomass degradation sugar.

The invention discloses difunctional xylanase as well as a preparation method and application thereof. According to the bifunctional xylanase, a C-terminal Dockeren structural domain is knocked out on the basis of wild type xylanase Xyn1G3 as shown in an amino acid sequence SEQ ID NO. 1, and the amino acid sequence of the bifunctional xylanase comprises a sequence as shown in SEQ ID NO. 3. A novel bifunctional enzyme with a simplified structure and optimized functions is successfully constructed by directional knockout of a Dockeren structural domain through a genetic engineering means, the bifunctional xylanase not only retains the original activity of xylanase, but also shows remarkable activity of cellulose hydrolase, and the application potential of the bifunctional xylanase in biomass degradation is expanded.

This invention belongs to the field of genetic engineering enzyme preparation technology, and particularly relates to a high-expression xylanase, gene editing, recombinant engineered bacteria, and their applications. This invention involves targeted amino acid site mutations in the wild-type xylanase Xyn-WT to obtain five mutant xylanases. Compared to the wild-type xylanase, the enzyme activities of all five mutants are significantly increased. Among them, the Xyn-4 mutant exhibits the best enzyme activity, reaching 245.17 U / mL, an increase of 90.60% compared to the wild type. Codon optimization further increased the expression level by 20.74%, demonstrating both high expression and excellent catalytic activity. The xylanase of this invention has high yield and high enzyme activity, making it suitable for industrial production scenarios such as feed, food, pulp and paper making, biomass degradation, and textiles, and possessing extremely high industrial application value.

The utility model relates to the technical field of biomass degradation hydrogen production, in particular to a high-efficiency hydrogen production double-chamber electrochemical membrane bioreactor which comprises a reactor main body, a cathode chamber and an anode chamber are arranged in the reactor main body, a proton exchange membrane is arranged between the cathode chamber and the anode chamber, and the anode chamber is communicated with the cathode chamber. A cathode and an anode are respectively arranged in the cathode chamber and the anode chamber, the cathode and the anode are connected with a weak current strengthening power supply, and a gas collecting port is formed in the top of the cathode chamber and is connected with a gas collecting device. The double-chamber electrochemical membrane bioreactor is formed by externally connecting a power supply and arranging the proton exchange membrane in the reactor main body, and the proton exchange membrane can effectively transfer protons and prevent the flow of electrons, reduce the waste of energy and unnecessary chemical reaction, improve the electrolytic efficiency, ensure that only hydrogen is generated at the cathode, and improve the electrochemical efficiency. The hydrogen purity and the hydrogen production efficiency are improved.

The present invention discloses a method and application for directly utilizing lignocellulose to generate electricity, and relates to the field of microbial fuel cells. The present invention can solve the problem of ineffective utilization of waste biomass, especially the problem that lignocellulose cannot be directly utilized in MFCs. The specific method of the present invention comprises pretreating a lignocellulose raw material to obtain a pretreated substrate; activating Acetovibrio thermocellum to obtain a seed liquid; injecting the seed liquid into a dual-chamber microbial fuel cell using the pretreated substrate as an electron donor, and outputting electrical energy from the electrodes. The present invention achieves the coupling of waste biomass degradation and electricity production, has low production costs, high electricity generation efficiency, avoids secondary environmental pollution, and has high economic value and broad application prospects in the energy conversion of lignocellulose.

The invention provides a reagent for oxidative degradation of lignin and a method for efficiently removing lignin, and belongs to the technical field of agricultural and forestry biomass degradation and conversion. The invention provides a reagent for oxidative degradation of lignin. The reagent is prepared from the following raw materials: a hydrogen peroxide solution, alkyl acetate and a hydrolysis catalyst, the concentration of the hydrogen peroxide solution is 8-15 wt%; the volume ratio of the hydrogen peroxide solution to the alkyl acetate is (4-6): (6-4); the volume of the hydrolysis catalyst accounts for 1.0-1.5% of the total volume of the hydrogen peroxide solution and the alkyl acetate. According to the method, a hydrogen peroxide-alkyl acetate system is established with the hydrogen peroxide solution with the concentration of 8-15 wt%, peracetic acid can be generated in situ, the reaction system is free of pressure and potential safety hazards during oxidation, and wood lignin can be removed with high selectivity under the condition that the temperature is larger than or equal to 60 DEG C.

The present invention discloses a thermostable xylanase mutant and its preparation method and application, and relates to the field of genetic engineering and genetic engineering technology. Hortaea werneckii Using the 10th Family xylanase HwXyl10A as the parent, point mutagenesis was used to screen for mutants N318W and D372L. Both mutants exhibited significantly improved thermostability and catalytic activity. The present invention identifies two key amino acid residues that influence the thermostability of Family 10 xylanases, providing important guidance for improving the thermostability of Family 10 xylanases. It also provides a basis for the industrialized production of xylanases and their application in feed preparation and biomass degradation of xylan to produce sugars.

The invention provides a high-activity pectin methylesterase mutant designed based on a large language model as well as preparation and application of the high-activity pectin methylesterase mutant. The amino acid sequence of the pectin methylesterase mutant (PME-DS7) is shown as SEQ ID NO: 2. According to the invention, a pectin methylesterase sequence is redesigned by using a large language model ESM-1v, and a mutant PME-DS7 with significantly improved enzymatic activity is screened in combination with multi-sequence alignment (MSA) analysis. Compared with wild pectin methylesterase, the enzymatic activity of the PME-DS7 is improved by about 2.8 times, and the PME-DS7 has higher industrial application value. The mutant has wide application prospects in the fields of food processing, spinning, papermaking, biomass degradation, improvement of raw material conversion efficiency and the like.

The invention provides neutral heat-resistant xyloglucanase MtXG and an application of the neutral heat-resistant xyloglucanase MtXG. Belongs to the technical field of gene engineering. According to the invention, the neutral heat-resistant xyloglucanase MtXG and the gene of the neutral heat-resistant xyloglucanase MtXG are successfully cloned from a thermophilic fungus Mycothermophilus CGMCC (China General Microbiological Culture Collection Center) 3.4393, and the neutral heat-resistant xyloglucanase MtXG and the gene are subjected to heterologous expression in pichia pastoris. Research results show that the MtXG not only shows the optimal enzymatic activity at the neutral pH of 7.0 and the high temperature of 75 DEG C, but also keeps good stability under wide pH and temperature conditions, and has high specificity and good heavy metal ion tolerance to xyloglucan at the same time. The MtXG is integrated into a commercial cellulase system, so that the yield of reducing sugar in tobacco leaves is greatly increased, the capability of improving the efficiency of a biomass degradation process is shown, and the MtXG has a good industrial application prospect.

The invention belongs to the technical field of microbial metabolism engineering, and particularly relates to a lignin degrading bacterium, a separation method, a microbial agent and application of the lignin degrading bacterium in promoting biomass degradation. The strain is named as Trabulsiella odontobacteria L1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35692. The invention further discloses a preparation method of the strain. The invention provides a lignin degrading bacterium L1 which can degrade lignin under both an aerobic condition and an anaerobic condition, so that the degradation and conversion of biomass are promoted, and the yield is increased. Therefore, compared with the prior art, the lignin degrading bacterium L1 disclosed by the invention is unique in function, low in oxygen demand and beneficial to lignin degradation demands in different scenes.

The invention discloses a biomass degradation foaming film bag and a preparation method thereof. The biomass degradable foaming film bag comprises the following components in parts by weight: 100 parts of a degradable polymer, 10-30 parts of a biomass foaming agent, 1-10 parts of an auxiliary foaming agent and 1-5 parts of a surface modifier, wherein the raw materials of the biomass foaming agent comprise biomass straws and sodium silicate. According to the invention, a self-developed biomass foaming agent is added into a degradable polymer matrix, and a simple double-screw melt extrusion processing mode is matched, so that the physical foaming process of water molecule volatilization can be completed, the quantity of generated bubbles is sufficient, and bubble holes are not easy to collapse; further, on the premise that the mechanical property of the biomass degradable foaming film bag is guaranteed, the product density is reduced, the resource utilization rate is increased, and compared with a traditional foaming agent, the used biomass foaming agent better meets the requirements for environmental protection and energy conservation and has important significance for replacing the traditional foaming agent.

The xylanase mutant and its application are obtained by mutation of Ser21 and Thr324 sites of the parent HwXyl10A. The xylanase mutant provided by the present invention has greatly improved thermal stability. The half-life of mutants S21Y and T324V at 75°C is ( t 1 / 2 ) were 88 min and 34 min, which were 14.7 times and 5.7 times that of the wild type (6 min), respectively; the mutants S21Y and T324V T 50 The optimal pH and temperature were 80.0°C and 76.5°C, respectively, 12.0°C and 8.5°C higher than the wild-type (68.0°C). The optimal pH and temperature were essentially the same as those of the wild-type, fully meeting the requirements for feed production and biomass degradation. This high-specific-activity thermostable xylanase mutant has broad application prospects in feed additives and biomass degradation for sugar production.

The application discloses a high-specific-activity endo-beta-1,4-glucanase EGH1, a preparation method and application thereof. The amino acid sequence of the EGH1 is shown as SEQ ID NO. 4. The specific enzyme activity of the enzyme is as high as 101.54 U / mg under the condition of pH=5.5 and 50 DEG C, and is significantly higher than that of commercial cellulase. Meanwhile, the enzyme activity of the EGH1 can be activated by Fe 2+ , and the enzyme activity is increased by about 60%. The enzyme can efficiently degrade natural lignocellulose without pretreatment, and the glucose release amount of the enzyme to the untreated bagasse is superior to that of the commercial enzyme under the same enzyme activity load, thus breaking the technical bottleneck that the existing cellulase seriously depends on a pretreatment process. In addition, the enzyme has the characteristics of good thermal stability, wide pH adaptation range and excellent metal ion tolerance, and can be widely applied to the biomass degradation process in the industries of biofuel, feed, textile and papermaking, and effectively reduces the production cost.

The application belongs to the field of environment and material technology, and discloses a method for optimizing pore channels of magnesium oxide modified biochar, which increases micropore volume in biochar through biomass degradation pretreatment, increases macropore volume in biochar through magnesium nitrate microwave foaming, increases mesopore volume in biochar through magnesium nitrate in-situ etching pyrolysis, realizes micropore, mesopore and macropore structure regulation in porous magnesium modified biochar through the synergistic effect of biomass degradation, in-situ etching and microwave foaming, and realizes hierarchical pore channel optimization. The application realizes the construction of porous structure magnesium modified biochar through the combined action of alkali solution, magnesium nitrate and magnesium acetate, wherein the magnesium nitrate microwave foaming and pyrolysis etching can respectively improve the macropore and mesopore structure of the material, and play a key role in the improvement of the pore structure and adsorption performance of the material. The method has the advantages of simplicity, easy industrialization, high control precision, excellent performance and the like.

The application discloses a kind of super double-tube spraying method and its application in biomass degradation, fungus growth, wrap deposition and the like.The super double-tube spraying method includes using high-pressure spray gun, and spraying mist drop after super double-tube, namely super double-tube spraying, wherein the super double-tube is PMMA polymer tube with super double-tube coating on inner wall, and the raw material for preparing the super double-tube coating includes TiO2 nanoparticle and hydrophobic epoxy resin.The application introduces super double-tube spraying technology, and by combining contact charging (CE) and induced charging (IE) effect, a kind of super double-tube spraying (SAP T-spraying) method is developed, which not only simplifies and optimizes the design of spraying, but also significantly improves the charge mass ratio (CMR) of mist drop, to realize efficient deposition on super-hydrophobic blade surface.