49 results about "Biomass degradation" patented technology

The invention discloses an in situ bioremediation method for strengthening the petroleum contaminated soil by biomass which belongs to the environment restoration technique. The method utilizes the biomass added with biomass degradation fungi to promote the conversion of biomass in polluted soil. The intermediate product transformed by biomass in soil is utilized to serve as the preferential carbon source of the petroleum hydrocarbon degradation complex bacteria for promoting the biological accumulation of the petroleum hydrocarbon degradation complex bacteria; meanwhile, the imposed petroleum hydrocarbon degradation complex bacteria contains the fungus that can produce biological surface active agent efficiently, the fungus produces abundant biological surface active agent during growth to activate the petroleum hydrocarbon pollutants in polluted soil for increasing the biological utilize degree, thus promoting the biotransformation efficiency of the petroleum hydrocarbon degradation complex bacteria to pollutants. Humus of the transformation product of the biomass in the soil can also improve the granular structure of the soil, and increase the fertility of the soil, so the polluted soil can be restored. The method is simple and is easy to operate and promotion.

The invention discloses an in situ biological repairing method of biomass strengthening petroleum -salt-mixed contaminated soil, which is to add biomass degradation agent into biomass, and then the biomass degradation agent is plowed and landfilled under plough horizon, water logging and salt washing are utilized to reduce soil salt content, and then petroleum hydrocarbon degradation bacteria agent is added to degrade petroleum hydrocarbon, The invention utilizes the biomass to interdict capillary salt uprise phenomenon possibly generated; carbohydrate substance generated by biomass degradation can be taken as a good quality carbon source of the petroleum hydrocarbon degradation bacteria agent, so as to accelerate the growth of the petroleum hydrocarbon degradation bacteria agent; lignin generated by the biological degradation can absorb petroleum hydrocarbon pollutant, to prevent being diffused in the water logging and salt washing, bio surfactant generated by microorganism in the petroleum hydrocarbon degradation bacteria agent can accelerate the digestion of the petroleum hydrocarbon pollutant and further be converted into carbon dioxide and water by the petroleum hydrocarbon degradation bacteria agent biology. The efficiency of the degradative petroleum hydrocarbon pollutant of the invention method is high, and the speed is quick.

The invention relates to a method for preparing a polyurethane foam material from biomass waste and papermaking black liquor extract serving as main raw materials. The low-cost and readily available biomass waste is taken as the raw material, the liquefaction product of the biomass waste is used for completely replacing polymer polyol, and waste produced in agricultural production is fully utilized; in addition, lignosulfonate is extracted from papermaking black liquor and added into biomass degradation liquor, a method for properly treating the papermaking black liquor is found while raw material cost is reduced, and the prepared polyurethane foam material has high degradation property due to the addition of a biomass component, and cannot pollute environment after being used. The waste in the agricultural production and the papermaking industry is effectively utilized, the raw material cost is greatly reduced, a new way is found for increasing the value of the waste produced in the agricultural production and the papermaking industry, inexhaustible plant resources can be fully utilized, and a road is opened up for recycling plants.

The invention discloses a microbial electrolysis two-section type sludge anaerobic digestion device and a method for producing methane by using the microbial electrolysis two-section type sludge anaerobic digestion device, relating to an improved two-section type anaerobic digestion device and a method for producing methanol from sludge. The purpose of the microbial electrolysis two-section type sludge anaerobic digestion device is to solve the technical problems of long fermentation cycle, low methanol yield and difficult biomass degradation and application in an existing residual sludge anaerobic digestion process. The microbial electrolysis two-section type sludge anaerobic digestion device consists of a continuous stirring type acidogenic reactor and a microbial electrocatalysis assisted upflow methane-producing reactor. The method comprises the following steps: starting a microbial catalytic electrolysis unit; producing acid by fermenting the residual sludge; and producing methanol. According to the microbial electrolysis two-section type sludge anaerobic digestion device and method disclosed by the invention, an acid production stage in a treatment process of the residual sludge only needs 5 to 8 days, a methanol production stage adopts continuous flow operation, the hydraulic retention time can be reduced to less than 1 day, and the operation cycle of sludge anaerobic digestion can be greatly shortened by adopting the device and method disclosed by the invention. The device and method disclosed by the invention belong to the field of sludge anaerobic digestion.

The invention relates to a system for preparing viscose by pretreating corn straw through microbial co-culture. Corn straw peel obtained after corn straw stalks and leaves and peel are separated is used as a raw material, microbial co-culture of biomass degradation rumen microorganisms and a rumen microorganism degradation product or metabolite is achieved through fermentation of special equipment to degrade hemicellulose and unstable cellulose in the straw peel, the fermentation product can be separated in situ through regulation to achieve biological pretreatment on the corn straw peel, remaining high-crystallized lignocellulose is continuously cooked under the anaerobic environment to remove lignin and is subjected to cellulose dyeing or bleaching, viscose dissolution spinning is directly carried out on centrifuged cellulose pulp under the condition of certain water content, or melt spinning is carried out under the dry condition to prepare viscose. Usage of agentia for following cooking delignification is reduced through biological pretreatment, and then pollution is reduced; oxidization and blackening of pulp are reduced through anaerobic cooking, and then pollution is reduced; meanwhile, characteristics of fiber are kept to the maximum.

The present invention provides a lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology, and belongs to the field of fuel ethanol production through biomass degradation fermentation. According to the technology, lignocellulose being subjected to acidolysis and steam blasting pretreatment is directly added with a surfactant and a buffer liquid without detoxification, pre-enzymolysis is performed, Saccharomyces cerevisiae is inoculated, ethanol fermentation is performed, the enrichment of the surfactant, the fermentation inhibitor and the buffer agent is achieved while the ethanol is distilled after the fermentation, and by extracting the fermentation inhibitor, the coupling separation recovery of the surfactant, the buffer agent and the fermentation inhibitor is achieved. According to the present invention, the recovered surfactant and the recovered fermentation inhibitor can be re-used so as to reduce the pollution and effectively reduce the cost; the recovered toxic inhibitor can be prepared into the high value chemical product through the chemical conversion so as to improve the atom utilization rate during the biomass conversion process; and with the lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology, the industrialization of the cellulose ethanol is further promoted.

Belonging to the technical field of biomass degradation, the invention relates to a method for degrading oxidation lignin into a small molecular aromatic compound, and in particular relates to a method of breaking C-C bond by nontransition metal catalysis to degrade oxidation lignin into a small molecular aromatic compound. The method includes: firstly oxidizing oxidation lignin in an organic solvent with an oxidizing agent, then subjecting the obtained intermediate product to alcoholysis or hydrolysis under the action of a catalyst so as to obtain a small molecular aromatic compound, like methoxy substituted benzoic acid, methoxy substituted phenol, methoxy substituted benzoate and chain alcohol, etc. The method provided by the invention has the advantages of simple steps, mild reaction conditions, high conversion rate (up to 100%), no need for transition metals and the like, has effect on multiple connection modes of oxidation lignin, can reach the purpose of high selectivity and efficient degradation of lignin, and is conducive to large-scale industrial application.

The invention discloses a super-high-temperature-resistant and alkali-resistant beta-mannase gene. A base sequence of the beta-mannase gene is SEQ ID NO.1. The invention further discloses a nucleotide sequence SEQ ID NO.2 of super-high-temperature-resistant and alkali-resistant beta-mannase, and further discloses application of the super-high-temperature-resistant and alkali-resistant beta-mannase gene in preparation of the super-high-temperature-resistant and alkali-resistant beta-mannase. The obtained expression enzyme can widely endure alkaline (pH7-10) and high-temperature (80-100 DEG C) environments; and the specific activity of the purified expression enzyme can be up to 10400U/mg. The beta-mannase has super-high-temperature-resistant and alkali-resistant characteristics, and is suitable for application in the chemical industries such as biomass degradation, foods, feeds, papermaking and spinning.

The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

The invention provides a detoxification and fermentation method of large red algae biomass degradation liquid. The detoxification and fermentation method comprises the following steps: degrading the large red algae biomass to obtain the degradation liquid, adding an appropriate amount of molecular-sieve or non-molecular-sieve adsorbent having adsorption property as a detoxifying agent into the degradation liquid to carry out double detoxification or multiple detoxification on the degradation liquid so as to remove substances of preventing the activities of microorganisms in the degradation liquid, finally removing the detoxifying agent and producing bio-fuels or chemical intermediates by fermentation of the corresponding fermentation microorganisms. By virtue of experimental verification, compared with the existing fermentation method of algae, the detoxification and fermentation method disclosed by the invention has the advantages that the treatment process of the degradation liquid is simple and consumes less time; the detoxifying agent is low in cost, wide in resources and can be recycled; the fermentation of the microorganisms has the advantages of high starting speed, short time, high yield and less byproducts and the fermented products are easily separated and purified.

The invention discloses a method for degrading lignin and model compound thereof through photocatalytic oxidation in a binary ionic liquid system, under a mild condition and by a one-pot method. In the method, binary ionic liquid comprises disubstituted imidazole ionic liquid and sulfo functionalized imidazole ionic liquid. The method is characterized in that the lignin model compound can be oxidatively degraded under the condition of low temperature (below 100 DEG C), atmospheric air and natural visible light irradiation, the highest conversion rate is 98.3%, products are benzoic acid and phenol, and the highest yields are 91.7% and 76.8% respectively; the natural lignin is oxidatively degraded to obtain acidic, phenolic and aldehydic aromatic products such as 4-ethylbenzoic acid, phloroglucinol and 4-methylphenylacetaldehyde. The method is environment-friendly and high-efficiency, the used ionic liquid can be recycled, the reaction operation condition is mild, the energy consumptionis low, and a technology is environment-friendly and safe; by the method, the problems of high energy consumption, high temperature, high pressure and a strict reaction condition in the conventional lignin oxidation method and the like are solved, and a novel method support is provided for industrialization of biomass degradation.

The invention discloses a method for preparing furoic acid and 5-hydroxymethyl furoic acid by biomasses. The method includes the steps: (1) taking oxygen-containing gas as an oxidizing agent, and converting the biomasses into degradation liquid containing gluconic acid and xylonic acid under hydrothermal reaction conditions through combined actions of a first homogeneous acid catalyst and a supported metal catalyst; (2) placing the degradation liquid containing the gluconic acid and the xylonic acid into an inert atmosphere, sequentially adding an organic solvent and a second homogeneous acidcatalyst to obtain mixed liquid, performing reaction on the mixed liquid to obtain the furoic acid and the 5-hydroxymethyl furoic acid. According to the method, a small amount of solid residue is generated in the whole reaction process, and the method avoids the problems of low target products, carbon deposition on device wall surface, pipeline blockage and the like caused by a lot of solid residue generated in traditional biomass degradation process.

The invention provides a preparation method for biomass degradation material, which comprises the following steps: (1) preparing raw materials: weighing the following compositions of raw materials by weight percentage: 40 to 60 of major ingredient poly( butylene succinate), 40 to 60 of modified material polylactic acid and 0.1 to 20 of filler calcium carbonate master batch; (2) mixing the compositions to form modified poly( butylene succinate) mixture; and (3) statically placing the modified mixture at low temperature for standby application. The invention also discloses a method for manufacturing a magnetic powder catheter by adopting the raw materials, which comprises the following steps: (4) preparing a magnetic powder catheter extrusion main machine and a round die head matched with the main machine for use; (5) feeding the modified poly( butylene succinate) mixture into the extrusion main machine so as to manufacture a continuous round hollow magnetic powder catheter; and (7) cutting the continuous round hollow magnetic powder catheter into magnetic powder catheter sections. The invention also provides the magnetic powder catheter prepared by the method. The materials provided by the invention can completely degrade. The method is scientific and reasonable; the production efficiency is high; the product performance is good; and the cost is low.

The invention provides a surfactant recovery technology for a lignocellulose hydrolysate fermentation process, and belongs to the field of biomass degradation fermentation production of fuel ethanol. According to the technology, detoxification treatment is not required to be performed on a lignocellulose hydrolysate subjected to thermochemical pretreatment such as acid hydrolysis or steam explosion, the pH value is directly regulated, a surfactant is added, brewer yeasts are added for ethanol fermentation, and then yeast cells are directly separated and recovered; enrichment of the surfactant, a fermentation inhibitor and a buffering agent is implemented at the same time of ethanol distillation; the fermentation inhibitor is extracted to implement coupling separation and recovery of the surfactant, the buffering agent and the fermentation inhibitor. The technology has the advantages that not only is the cyclic utilization efficiency of the yeasts improved, but also coupling recovery of the surfactant, the buffering agent and the fermentation inhibitor is implemented; relative to an ethanol distillation process, no energy consumption is added by surfactant recovery, a recovery process is simple, pollution is effectively reduced, the cost is lowered, and the industrialization process of cellulosic ethanol is further promoted.

The invention discloses a method for preparing organic acid by oxidative degradation of biomass and a prepared organic acid, which relate to the field of biomass reuse and solve the problems of high energy consumption, high cost, harsh reaction conditions, large residue amount and environment pollution existing in conventional biomass degradation and utilization methods. The method comprises the following steps: biomass and acetic anhydride are uniformly mixed according to a solid-liquid ratio of 1g:(5ml to 30ml) under magnetic stirring, and 30 percent of hydrogen peroxide is dripped by a constant-pressure drop funnel; the mixture is oxidized under the condition of 35 DEG C to 85 DEG C for 6 to 48 hours; filtration is performed to obtain an oxidized solution; the oxidized solution is driedby evaporation in water bath, so as to obtain residue; and the residue is separated by column chromatography, so that the organic acid is obtained. By oxidatively degrading an organic matter in the biomass under mild conditions, the chemical with high added value-the organic acid can be obtained, establishing a novel method for the application of biomass. The method has the advantages of low energy consumption, low cost, less residue and no environment pollution.

The invention relates to a BdREF2 gene for regulating asafetide anabolism and application of the BdREF2 gene, and belongs to the field of gene engineering. According to the BdREF2 gene disclosed by the invention, a coniferous aldehyde dehydrogenase gene named BdREF2 is separated from cDNA library of brachypodium distachyon; the BdREF2 gene in a brachypodium distachyon genome is knocked out by adopting a CRISPER/Cas9 gene editing system; it is found that the mutation of the BdREF2 can decrease the content of ferulic acid ester in plant cell walls while the degradation efficiency of biomass is obviously improved; the result indicates that the BdREF2 gene can be applied to biomass biodegradable genetic improvement of gramineous plants. It is firstly and directly proved that an REF homologousgene involved in regulating the biosynthesis of ferulic acid can be applied to improving the genetic improvement on biomass degradation and transformation efficiency of the gramineous plants, and hasimportant value for resource efficient utilization of biomass such as gramineae energy grass, forages and crop straws.

The invention discloses garbage treatment equipment for biodegradation, and relates to the technical field of biomass degradation and conversion equipment. The garbage treatment equipment for the biodegradation comprises a recycling barrel, a relieving mechanism is arranged on the inner surface of the recycling barrel, a collecting barrel is slidably connected to the inner surface of the recycling barrel, a through groove is arranged on the inner surface of the collecting barrel, a screening window is slidably connected to the inner surface of the through groove, and a one-way mechanism is arranged on the inner surface of the screening window. The collecting barrel capable of moving up and down to separate solid from liquid is additionally arranged in a barrel for containing garbage in a centralized mode, solid-liquid separation is carried out on the garbage through vertical movement of the collecting barrel and centrifugal force generated by operation of internal components, force capable of relieving ascending and descending is added at the lifting position, the safety of equipment in the using process is further improved, the functionality of the equipment is also improved, and after the garbage is collected, solid-liquid separation can be directly carried out, and the garbage can be compacted, so that the internal space of the equipment can be fully utilized during collection.

The present invention relates to recombinant bacteria and the uses thereof, particularly for the production of ethanol. The invention also relates to methods for the production of such bacteria, as well as to nucleic acid constructs suitable for such production. The invention specifically relates to bacteria having a reconstructed biomass degradation unit.

The invention discloses a production technology of cello-oligosaccharide. The technology comprises the following basic steps: (1) crushing a biomass raw material (2) uniformly mixing the crushed sample and a reaction solution; (3) letting the reaction system react at room temperature for a certain period of time; (4) adding water to dilute a reaction liquid; (5) hydrolyzing the reaction system at high temperature for a period of time; (6) carrying out solid-liquid separation, and neutralizing a liquid part by the use of alkali; and (7) carrying out sugar-salt separation to obtain cello-oligosaccharide. The operation method is simple, and the problem that molecular weight cannot be manually controlled at a stage by pure biomass degradation is solved. The production technology makes it possible to realize industrial large-scale production of cellulose-enriched biomass and obtain high-purity cello-oligosaccharide.

The invention provides GH10 family high-temperature-resistant xylanase mutants and application thereof. The mutants are HwXyl10A-G363R, HwXyl10A-T324V, HwXyl10A-N318W and HwXyl10A-N318W/G363R/T324V mutants; and the half-life periods of the four mutants at 75 DEG C are prolonged by 12min, 20min, 24min and 38min respectively compared with those of wild mutants. The xylanase mutants have high catalytic activity and excellent thermal stability at animal body temperature and have great application potential in feed addition and biomass degradation.

The invention provides a method for removing poplar lignin by cyclic utilization of acetic acid-hydrogen peroxide pretreatment liquid, and belongs to the technical field of forestry biomass degradation and conversion. The method comprises the following steps: mixing an acetic acid-hydrogen peroxide solution with poplar and sulfuric acid to obtain a mixture; treating the mixture at 60-80 DEG C for2 hours, and carrying out solid-liquid separation to obtain a liquid, namely a pretreatment liquid; and mixing the pretreatment solution with the poplar, and repeating for 3-4 times to realize cyclicutilization of the pretreatment solution. According to the method, the poplar is treated with the acetic acid-hydrogen peroxide pretreatment liquid, consumption of acetic acid-hydrogen peroxide is reduced, and the pretreatment cost is reduced; acetic acid and hydrogen peroxide are added into the pretreatment liquid to prolong the use frequency of the pretreatment liquid, and the supplemented reagents are non-toxic and harmless; the problems of complex recovery process, high cost and serious pollution of the pretreatment liquid are solved; 57% of poplar lignin can still be removed after the pretreatment liquid is repeatedly used for three times, and the yield of enzyme hydrolysis glucose of the pretreated poplar can reach 90.0% or above.

The invention belongs to the technical field of bioengineering, and particularly relates to a bacterial-derived lytic polysaccharide monooxygenase and application thereof. The amino acid sequence of the PlLPMOCB3 is as shown in SEQ ID NO. 1 in a sequence table. The PlLPMOCB3 not only has the capability of degrading biomass polysaccharides such as cellulose, xylan, chitin and the like, but also has a synergistic effect with commercial cellulase, xylanase or chitinase to improve the degradation efficiency of cellulose, lignocellulose, xylan and chitin substrates. Wherein when PlLPMOCB3 and commercial cellulase act together, the synergy degree of cellulose and corncob substrates can reach 1.43 and 1.22 respectively; when the xylanase acts together, the synergy degree reaches 4.57; and the synergism degree is up to 1.70 when the bacillus subtilis and chitinase act together. And a new way is provided for enriching a lysable polysaccharide monooxygenase library and artificially compounding a biomass degradation efficient compound enzyme system.