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Lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology

A technology of synchronous saccharification and fermentation and surfactants, applied in recycling technology, fermentation, plastic recycling, etc., can solve the problems of cellulase and Saccharomyces cerevisiae that cannot work normally, and achieve the effect of improving atom utilization and wide application prospects

Active Publication Date: 2017-03-22
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These compounds have obvious inhibitory effects on cellulase activity, yeast growth and fermentation during the enzymatic hydrolysis process, resulting in the failure of cellulase and Saccharomyces cerevisiae to work normally, becoming the main obstacle to the production of cellulosic ethanol

Method used

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  • Lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology
  • Lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Mix 1.5g of pretreated fast-growing poplar powder as a substrate, 0.2g / ml PEG-1000, 12ml of buffer, cellulase 20FPU / g raw material, pre-enzymolyze at 50°C for 24 hours, and then lower the temperature to 33°C , and then add Saccharomyces cerevisiae to keep the cell concentration at 0.8*10 8 / mL for 72 hours of SSF fermentation. Carry out solid-liquid separation after fermentation, distill and extract the supernatant, and the remaining liquid after distillation is surfactant and buffer, which can be directly used in the next fermentation process (such as figure 1 ). PEG-1000 cycled 4 times the result is as follows figure 2 shown. It can be seen from the figure that PEG-1000 can be recycled and reused, and the concentration of ethanol is basically constant.

Embodiment 2

[0031] The experimental procedure is the same as that of Example 1, except that PEG-200 and ethanol concentration are shown in Table 1. It can be seen from the table that PEG-200 can be recycled and reused.

[0032] Table 1 Ethanol concentration data

[0033] PEG-200 Fresh cycle 1 cycle 2 cycle 3 Ethanol concentration [g / l] 17.0 18.25 16.20 15.08

Embodiment 3

[0035] The experimental procedure is the same as that of Example 1, except that 0.2 g / ml PEG-400 is added, and the concentration of ethanol is shown in Table 2. It can be seen from the table that PEG-400 can be recycled and reused.

[0036] Table 2 Ethanol concentration data

[0037] PEG-400 Fresh cycle 1 cycle 2 cycle 3 Ethanol concentration [g / l] 21.20 20.35 18.51 19.68

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Abstract

The present invention provides a lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology, and belongs to the field of fuel ethanol production through biomass degradation fermentation. According to the technology, lignocellulose being subjected to acidolysis and steam blasting pretreatment is directly added with a surfactant and a buffer liquid without detoxification, pre-enzymolysis is performed, Saccharomyces cerevisiae is inoculated, ethanol fermentation is performed, the enrichment of the surfactant, the fermentation inhibitor and the buffer agent is achieved while the ethanol is distilled after the fermentation, and by extracting the fermentation inhibitor, the coupling separation recovery of the surfactant, the buffer agent and the fermentation inhibitor is achieved. According to the present invention, the recovered surfactant and the recovered fermentation inhibitor can be re-used so as to reduce the pollution and effectively reduce the cost; the recovered toxic inhibitor can be prepared into the high value chemical product through the chemical conversion so as to improve the atom utilization rate during the biomass conversion process; and with the lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology, the industrialization of the cellulose ethanol is further promoted.

Description

technical field [0001] The invention relates to the field of producing fuel ethanol through a fermentation process through the degradation of biomass raw materials, in particular to a surfactant recovery technology in the synchronous saccharification and fermentation process of lignocellulose. Background technique [0002] Lignocellulose is the most abundant biomass resource in the world. The production of fuel ethanol from lignocellulose can not only effectively alleviate the energy crisis and reduce environmental pollution, but also meet the sustainable development goal of large-scale replacement of petroleum by fuel ethanol in the future. economic value and social significance. At present, lignocellulose simultaneous saccharification and fermentation (SSCF) process is widely used in the production of cellulosic ethanol. However, the production of fuel ethanol from lignocellulose must be pretreated. The conditions of high temperature and high pressure in the pretreatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C08J11/04C12R1/865
CPCY02E50/10Y02P20/143Y02W30/62
Inventor 张宗超刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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