This invention discloses a centrifugal
microfluidic chip and a
nucleic acid extraction method, specifically relating to the field of
nucleic acid extraction technology. The method includes a pretreatment
filtration module and a
nucleic acid extraction and purification module. The sample solution undergoes primary and secondary
filtration in the pretreatment
filtration module to remove particulate impurities and
protein precipitates, obtaining a clear sample supernatant. Nucleic acids in the supernatant are captured by a
silica membrane in the nucleic acid extraction and purification module. Subsequently, a first washing solution and a second washing
solution flow sequentially through the
silica membrane to wash the adsorbed nucleic acids, removing residual impurities and reaction inhibitors. Finally, the eluent flows through the
silica membrane, carrying the nucleic acids into the nucleic acid collection chamber, thereby obtaining the purified nucleic acid sample. This invention features a simple structure and reliable control, enabling
sequential control of liquids in multiple chambers, effectively removing impurities and inhibitors from the sample, improving nucleic acid extraction efficiency and purity, and achieving a high degree of integration and
automation in the nucleic acid extraction process for soil samples.