3results about How to "Efficient catalytic activity" patented technology

This invention provides a multi-site mutant of cocaine esterase, its protein dimer, and its applications. By mutating cysteine ​​(C) at position 107 to serine (S), and cysteine ​​(C) at position 551 to serine (S), arginine (R), or alanine (A), it achieves efficient and uniform cross-linking with BMOE while maintaining cocaine catalytic activity, forming a structurally stable dimer. This modification significantly improves the enzyme's thermostability and prolongs its half-life in vivo. The protein dimer is suitable for the treatment and prevention of acute cocaine poisoning.

This invention relates to the field of enzyme engineering technology, and in particular to a total synthetic ketone reductase and its applications. The amino acid sequence of the total synthetic ketone reductase is shown in SEQ ID NO:2 or SEQ ID NO:3. This total synthetic ketone reductase can be used to prepare (S)-3-hydroxytetrahydrofuran. Specifically, recombinant engineered bacteria are cultured, lysed, and added to a reaction system containing substrate compound II, NAD+, and isopropanol. The reaction yields compound I. The specific route is as follows: the recombinant engineered bacteria are *E. coli* BL21(DE3) as the host and pET-30a(+) as the vector, expressing the above-mentioned total synthetic ketone reductase. The ketone reductase of this invention can stably express heterologous functionality, and it exhibits highly efficient catalytic activity in the preparation of the chiral compound (S)-3-hydroxytetrahydrofuran, demonstrating significant potential for industrial application.

The invention discloses a preparation method of a boron-doped titanium-based supported catalyst, and belongs to the technical field of catalytic materials and organic synthesis.The preparation method of the boron-doped titanium-based supported catalyst comprises the steps that tetrabutyl titanate and terephthalic acid serve as precursors, a titanium-containing MOF precursor is synthesized through a solvothermal method, then a boron source is introduced to achieve in-situ doping of the boron element, and the boron-doped titanium-based supported catalyst is obtained. Then loading metal on the boron-doped MOF precursor in a monatomic form through an impregnation-coordination-high-temperature pyrolysis process; according to the invention, the electron structure and pore characteristics of the carrier are regulated and controlled through boron doping, and the catalyst is endowed with excellent catalytic performance in combination with high exposure active sites of monatomic metal; the catalyst can efficiently catalyze nitro compounds to be reduced to generate corresponding amines under the conditions of no solvent or existence and mild hydrogenation, the yield of target products is larger than 90%, the conversion rate is larger than 95%, the catalyst is simple in preparation process and high in stability, pollution and safety risks caused by organic solvents can be avoided, the requirements of green chemical development are met, and the method is suitable for industrial production. The method is suitable for large-scale production in the fields of fine chemical engineering, pharmacy and the like.