Preparation method and application of shewanella spp-nanometer ferrous sulfide capsules

A technology of Shewanella and ferrous sulfide is applied in the field of preparation of Shewanella-nano ferrous sulfide capsules, which can solve the problems of difficult multi-cycle treatment, strong microbial toxicity and high operating cost, and achieves efficient multi-cycle treatment, Improve processing efficiency and increase the effect of reducing sites
CN109735527AActive Publication Date: 2019-05-10JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2019-05-10

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Abstract

The invention belongs to the technical field of biology, and relates to a preparation method and application of shewanella spp-nanometer ferrous sulfide capsules. The method comprises the steps that an LB culturing medium and an M9 culturing medium are prepared, shewanella spp is inoculated to the LB culturing medium for culturing, and bacterial liquid is obtained; the LB culturing medium and theM9 culturing medium are mixed, sodium lactate, calcium chloride and magnesium sulfate are added, and reaction buffering liquid is obtained; then, the bacterial liquid is centrifuged, bacterial sludgeis added into the reaction buffering liquid until the OD 600 value is 0.05-5; then, a ferric chloride solution and a sodium thiosulfate solution are added into the reaction buffering liquid after inoculation of the bacterial sludge, and after culturing, centrifugation, washing and resuspending, the shewanella spp-nanometer ferrous sulfide capsules are obtained; in the process of synthesizing the capsules, the activity of the biological metabolism mechanism is fully utilized, the raw materials are simple and cheap, the application cost is low, and quantization production can be achieved; multi-period efficient removal for hexavalent chromium is efficiently achieved, and a new method is provided for efficiently processing heavy metal pollution.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of Shewanella-nano ferrous sulfide capsules and application thereof. Background technique

[0002] In recent years, with the acceleration of industrialization and urbanization, as important raw materials in metallurgy, electroplating, chemical industry, dyestuff and other industries, chromium and its compounds have been widely used. A large amount of chromium-containing wastewater, chromium-containing slag, and chromium-containing dust enters the water body, causing serious environmental pollution. As a heavy metal element, chromium element mainly exists in the form of divalent, trivalent and hexavalent in the environment, mainly in the form of trivalent chromium and hexavalent chromium in water, and trivalent chromium exists in the form of hydrated ions, with low toxicity , Low solubility in water and easy precipitation; Hexavalent chromium is easil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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