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Preparation method and application of shewanella spp-nanometer ferrous sulfide capsules

A technology of Shewanella and ferrous sulfide is applied in the field of preparation of Shewanella-nano ferrous sulfide capsules, which can solve the problems of difficult multi-cycle treatment, strong microbial toxicity and high operating cost, and achieves efficient multi-cycle treatment, Improve processing efficiency and increase the effect of reducing sites

Active Publication Date: 2019-05-10
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical reduction method is mainly to add a reducing agent. Although this method reacts quickly, it consumes a lot and is prone to produce a large amount of toxic substances, causing secondary pollution of the water body. In contrast, although physical chemical methods and electrochemical methods do not produce A large number of by-products, but high operating costs, which limit the application of traditional methods
In the emerging field of nanomaterials, nano-ferrous sulfide is widely used in the treatment of chromium-containing wastewater due to its high catalytic efficiency and strong reducibility, but the main problems are the large consumption and high cost of nanomaterials.
However, the main problem at present is that the efficiency of microorganisms to treat hexavalent chromium is relatively low, and hexavalent chromium is highly toxic to microorganisms, so it is difficult to carry out multi-cycle treatment

Method used

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  • Preparation method and application of shewanella spp-nanometer ferrous sulfide capsules
  • Preparation method and application of shewanella spp-nanometer ferrous sulfide capsules
  • Preparation method and application of shewanella spp-nanometer ferrous sulfide capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Configure LB medium, including tryptone 10g / L, yeast extract 5g / L, sodium chloride 5g / L, pH=7; configure M9 medium, including Na 2 HPO 4 ·12H 2 O 17.8g / L, KH 2 PO 4 3g / L, NaCl 0.5g / L, NH 4 Cl 10.5g / L, 10mM sodium lactate, 0.1mM CaCl 2 and 1mM MgSO 4 Then Shewanella (purchased from ATCC American Type Culture Collection, bacterial classification number ATCC700550) is inoculated in the LB substratum and cultivated, and the inoculum amount and the LB substratum of the Shewanella inoculated in the LB substratum The volume ratio is 0.2:100; then placed at a temperature of 30 °C and a shaking speed of 200 rpm for 12 hours to obtain bacterial liquid;

[0038] (2) Preparation of the reaction buffer for the treatment of hexavalent chromium by Shewanella-nano ferrous sulfide capsules: fully mix the LB liquid medium and the M9 medium according to the volume ratio of 1:15 to obtain a mixed solution, and then take 30 mL of the mixed solution solution, and then add sodium ...

Embodiment 2

[0043] It is basically the same as Example 1, but has the following changes: do not add ferric chloride and sodium thiosulfate, measure the reduction situation of Shewanella to hexavalent chromium under the same conditions, and draw a degradation curve as a control (such as Image 6 a).

Embodiment 3

[0045] Basically the same as Example 1, but with the following changes: without adding ferric chloride and sodium thiosulfate, while inactivating Shewanella, single determination of the reduction of hexavalent chromium by dead Shewanella under the same conditions situation, and draw a degradation curve as a control (such as Image 6 a).

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Abstract

The invention belongs to the technical field of biology, and relates to a preparation method and application of shewanella spp-nanometer ferrous sulfide capsules. The method comprises the steps that an LB culturing medium and an M9 culturing medium are prepared, shewanella spp is inoculated to the LB culturing medium for culturing, and bacterial liquid is obtained; the LB culturing medium and theM9 culturing medium are mixed, sodium lactate, calcium chloride and magnesium sulfate are added, and reaction buffering liquid is obtained; then, the bacterial liquid is centrifuged, bacterial sludgeis added into the reaction buffering liquid until the OD 600 value is 0.05-5; then, a ferric chloride solution and a sodium thiosulfate solution are added into the reaction buffering liquid after inoculation of the bacterial sludge, and after culturing, centrifugation, washing and resuspending, the shewanella spp-nanometer ferrous sulfide capsules are obtained; in the process of synthesizing the capsules, the activity of the biological metabolism mechanism is fully utilized, the raw materials are simple and cheap, the application cost is low, and quantization production can be achieved; multi-period efficient removal for hexavalent chromium is efficiently achieved, and a new method is provided for efficiently processing heavy metal pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of Shewanella-nano ferrous sulfide capsules and application thereof. Background technique [0002] In recent years, with the acceleration of industrialization and urbanization, as important raw materials in metallurgy, electroplating, chemical industry, dyestuff and other industries, chromium and its compounds have been widely used. A large amount of chromium-containing wastewater, chromium-containing slag, and chromium-containing dust enters the water body, causing serious environmental pollution. As a heavy metal element, chromium element mainly exists in the form of divalent, trivalent and hexavalent in the environment, mainly in the form of trivalent chromium and hexavalent chromium in water, and trivalent chromium exists in the form of hydrated ions, with low toxicity , Low solubility in water and easy precipitation; Hexavalent chromium is easil...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34C12R1/01C02F101/22
CPCY02A50/30
Inventor 雍阳春程千文俞洋洋
Owner JIANGSU UNIV
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