Application of nano-nickel oxide as mimetic peroxidase for detecting hydrogen peroxide

A nanometer nickel oxide and peroxide technology, which is applied in the field of nanobiochemical detection, can solve the problems of volatile inactivation of natural enzymes, high storage and use costs, difficult purification and other problems, and can meet the requirements of production and application, high stability, Actionable effect

Inactive Publication Date: 2015-05-06
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Enzymes are efficient catalysts. However, natural enzymes have disadvantages such as variability and inactivation, high price, difficult purification, high storage and use costs, making the study of mimic enzymes an urgent task.

Method used

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  • Application of nano-nickel oxide as mimetic peroxidase for detecting hydrogen peroxide
  • Application of nano-nickel oxide as mimetic peroxidase for detecting hydrogen peroxide
  • Application of nano-nickel oxide as mimetic peroxidase for detecting hydrogen peroxide

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Synthesis of nano-nickel oxide:

[0030] Add 20mL of 1mol / L nickel nitrate salt solution dropwise into 1mol / L sodium carbonate (Na 2 CO 3 ) solution (Na 2 CO 3 The amount is just enough to completely precipitate nickel nitrate), react at 50°C for 1h, wash with deionized water until neutral, dry at 100°C, and roast at 300°C for 3h to obtain nano-nickel oxide particles with a particle diameter of about 4nm.

[0031] figure 1 It is the XRD pattern of the nano-nickel oxide prepared by the present invention.

Embodiment 2

[0033] Nano-nickel oxide was used as a peroxide mimetic enzyme to determine the content of hydrogen peroxide.

[0034] Add 50ml, 1mg / ml nickel oxide solution, 20ml (5mg / ml) TMB solution to the solution, and then add different amounts of H 2 o 2 , such that H 2 o 2 The final concentration is 0, 10 -9 , 5×10 -9 , 10 -8 , 5×10 -8 , 10 -7 , 5×10 -7 , 10 -6 , 5×10 -6 , 10 -5 , 5×10 -5 , 10 -4 , 5×10 -4 , 10 -3 , 5×10 -3 , 10 -2 (mol / L). Add sodium acetate buffer solution (pH=7.0), so that the final volume of the solution to be tested is 200ml. After 30 min at room temperature, the absorbance of the solution was measured using a microplate reader.

[0035] figure 2 In order to use TMB as the chromogen, nano-nickel oxide as the peroxide mimetic enzyme to determine the content of hydrogen peroxide.

Embodiment 3

[0037] Nano-nickel oxide was used as a peroxide mimetic enzyme to determine the content of hydrogen peroxide.

[0038] Add 50ml, 1mg / ml nickel oxide solution, 20ml (5mg / ml) ABTS solution to the solution, and then add different amounts of H 2 o 2 , such that H 2 o 2 The final concentration is 0, 10 -9 , 5×10 -9 , 10 -8 , 5×10 -8 , 10 -7 , 5×10 -7 , 10 -6 , 5×10 -6 , 10 -5 , 5×10 -5 , 10 -4 , 5×10 -4 , 10 -3 , 5×10-3 , 10 -2 (mol / L). Sodium acetate buffer solution (pH=4.0) was added so that the final volume of the solution to be tested was 200ml. After 30 min at room temperature, the absorbance of the solution was measured using a microplate reader.

[0039] image 3 Catalytic activity diagram of hydrogen peroxide catalyzed by nano-nickel oxide at room temperature using ABTS as color reagent.

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Abstract

The invention relates to the application of nano-nickel oxide as a mimetic peroxidase for detecting hydrogen peroxide. Nano-nickel oxide serves as the mimetic peroxidase and has the activity of the mimetic peroxidase; the particle size of nano-nickel oxide is not limited to 4nm, and nano-nickel oxide further contains nano-nickel oxide particles of other sizes. For the first time, nano-nickel oxide is found to have the function of the mimetic peroxidase and can be applied to detecting the content of hydrogen peroxide; compared with HRP, prepared nano-nickel oxide, serving as a catalyst, is high in stability and reusable, and has efficient mimetic peroxidase catalytic activity; in the absence of hydrogen peroxide, nano-nickel oxide is also capable of catalyzing a color developing agent, and can be used as a mimic enzyme; the preparation method of nano-nickel oxide is simple in process, high in operability, and capable of further satisfying production and application.

Description

technical field [0001] The invention relates to the application of nano-nickel oxide (NiO) as a peroxide mimetic enzyme in the determination of hydrogen peroxide. The invention belongs to the field of nanometer biochemical detection. Background technique [0002] Enzymes are efficient catalysts. However, natural enzymes have disadvantages such as variability and inactivation, high price, difficult purification, high storage and use costs, making the study of mimic enzymes an urgent task. [0003] Since the first discovery of magnetic ferric oxide nanoparticles (Fe 3 o 4 NPs) have peroxidase-like catalytic activity, that is, Fe 3 o 4 NPs can catalyze H 2 o 2 React with horseradish peroxidase substrates 3,3',5.,5'-tetramethylbenzidine (TMB), diazoaminobenzene (DAB), etc. to generate the same reaction product, showing the same reaction as HRP has a similar catalytic effect. Since then, various nanoparticles with peroxide-like enzyme-like properties have been synthesiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31C01G53/04B82Y30/00
Inventor 何丹农王萍赵昆峰夏智伟
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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