Application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide detection

A technology for the concentration of peroxide and hydrogen peroxide, which is applied in the field of nano-biochemical detection, can solve the problems of volatile inactivation of natural enzymes, high storage and use costs, and difficult purification, and can meet the requirements of production and application, and has high stability. , the effect of strong operability

Inactive Publication Date: 2015-11-11
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Enzymes are efficient catalysts. However, natural enzymes have disadvantages such as variability and inactivation, high price, difficult purification, high storage and use costs, making the study of mimic enzymes an urgent task.

Method used

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  • Application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide detection
  • Application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide detection
  • Application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide detection

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Synthesis of nano-manganese oxide

[0033] Add 20mL of 1mol / L manganese nitrate salt solution dropwise into 1mol / L sodium carbonate (Na 2 CO 3 ) solution (Na 2 CO 3 The amount is just enough to make the manganese nitrate precipitate completely), react at 50°C for 1h, wash with deionized water until neutral, dry at 100°C, and roast at 300°C for 3h to obtain nano-manganese oxide particles, manganese oxide is Mn 2 o 3 and a small amount of MnO 2 of mixed oxides.

[0034] figure 1 It is the XRD pattern of the nano-manganese oxide prepared by the present invention.

Embodiment 2

[0036] Nano-manganese oxide was used as a peroxide mimetic enzyme to determine the content of hydrogen peroxide.

[0037] Add 50ml, 1mg / ml manganese oxide solution, 20ml (5mg / ml) TMB solution to the solution, and then add different amounts of H 2 o 2 , such that H 2 o 2 The final concentration is 0, 10 -9 , 5×10 -9 , 10 -8 , 5×10 -8 , 10 -7 , 5×10 -7 , 10 -6 , 5×10 -6 , 10 -5 , 5×10 -5 , 10 -4 , 5×10 -4 , 10 -3 , 5×10 -3 , 10 -2 (mol / L). Add sodium acetate buffer solution (pH=7.0), so that the final volume of the solution to be tested is 200ml. After 30 min at room temperature, the absorbance of the solution was measured using a microplate reader.

[0038] figure 2 In order to use TMB as chromogen, nano-manganese oxide was used as peroxide mimetic enzyme to measure hydrogen peroxide content.

Embodiment 3

[0040] Nano-manganese oxide was used as a peroxide mimetic enzyme to determine the content of hydrogen peroxide.

[0041] Add 50ml, 1mg / ml manganese oxide solution, 20ml (5mg / ml) ABTS solution to the solution, and then add different amounts of H 2 o 2 , such that H 2 o 2 The final concentration is 0, 10 -9 , 5×10 -9 , 10-8 , 5×10 -8 , 10 -7 , 5×10 -7 , 10 -6 , 5×10 -6 , 10 -5 , 5×10 -5 , 10 -4 , 5×10 -4 , 10 -3 , 5×10 -3 , 10 -2 (mol / L). Sodium acetate buffer solution (pH=4.0) was added so that the final volume of the solution to be tested was 200ml. After 30 min at room temperature, the absorbance of the solution was measured using a microplate reader.

[0042] image 3 The catalytic activity diagram of nano-manganese oxide catalyzing hydrogen peroxide at room temperature using ABTS as color reagent.

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Abstract

The invention relates to an application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide concentration measurement. The application has the advantages that the nanometer MnOx and CrOx are discovered to have a peroxide mimic enzyme function for the first time, and can be used for detecting hydrogen peroxide content; the prepared nanometer MnOx and CrOx serving as catalysts have high stability, reusability and efficient peroxide mimic enzyme catalytic activity in comparison to HRP; the condition that without the existence of hydrogen peroxide, the nanometer MnOx and CrOx also can catalyze a color developing agent, and are used as mimic enzymes is discovered; a preparation method is simple in process, high in operability and capable of further meeting production and application needs.

Description

[0001] The invention relates to the application of nano manganese oxide and chromium oxide (MnOx, CrOx) as a peroxide mimetic enzyme to measure hydrogen peroxide. The invention belongs to the field of nanometer biochemical detection. Background technique [0002] Enzymes are efficient catalysts. However, natural enzymes have disadvantages such as variability and inactivation, high price, difficult purification, high storage and use costs, making the study of mimic enzymes an urgent task. [0003] Since the first discovery of magnetic ferric oxide nanoparticles (Fe 3 o 4 NPs) have peroxidase-like catalytic activity, that is, Fe 3 o 4 NPs can catalyze H 2 o 2 React with horseradish peroxidase substrates 3,3',5.,5'-tetramethylbenzidine (TMB), diazoaminobenzene (DAB), etc. to generate the same reaction product, showing the same reaction as HRP has a similar catalytic effect. Since then, various nanoparticles with peroxide-like enzyme-like properties have been synthesized an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 何丹农王萍夏智伟刘婷赵昆峰金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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