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Application of nanometer cobaltosic oxide used as peroxide enzyme mimic for measuring concentration of hydrogen peroxide

A technology of tricobalt tetroxide and peroxide, which is applied in the field of nano-biochemical detection, can solve the problems of high storage and use costs, the variability and inactivation of natural enzymes, and high prices, and meet the requirements of production and application, high stability, and catalytic reactions. dramatic effect

Inactive Publication Date: 2015-04-29
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages of natural enzymes, such as variability and inactivation, high price, difficult purification, high storage and use costs, make the study of mimic enzymes an urgent task.

Method used

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  • Application of nanometer cobaltosic oxide used as peroxide enzyme mimic for measuring concentration of hydrogen peroxide
  • Application of nanometer cobaltosic oxide used as peroxide enzyme mimic for measuring concentration of hydrogen peroxide
  • Application of nanometer cobaltosic oxide used as peroxide enzyme mimic for measuring concentration of hydrogen peroxide

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Experimental program
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Effect test

Embodiment 1

[0029] Synthesis of nano-cobalt tetroxide and its concentration as a mimetic enzyme.

[0030] Add 20mL of 1mol / L cobalt nitrate salt solution dropwise into 1mol / L sodium carbonate (Na 2 CO 3 ) solution (Na 2 CO 3 Just enough to make the cobalt nitrate precipitate completely), react at 50°C for 1h, wash with deionized water until neutral, dry at 100°C, and roast at 300°C for 3h to obtain nano-cobalt trioxide particles with a particle diameter of about 9nm.

[0031] figure 1 It is the XRD figure of the nano cobalt trioxide prepared by the present invention.

Embodiment 2

[0033] Nano-cobalt tetroxide is used as a peroxide mimetic enzyme to detect hydrogen peroxide content.

[0034] Add 50ml, 1mg / ml cobalt tetroxide solution, 20ml (5mg / ml) TMB solution to the solution, and then add different amounts of H 2 o 2 , such that H 2 o 2 The final concentration is 0, 10 -9 , 5×10 -9 , 10 -8 , 5×10 -8 , 10 -7 , 5×10 -7 , 10 -6 , 5×10 -6 , 10 -5 , 5×10 -5 , 10 -4 , 5×10 -4 , 10 -3 , 5×10 -3 , 10 -2 (mol / L). Add sodium acetate buffer solution (pH=7.0), so that the final volume of the solution to be tested is 200ml. Measure the absorbance of the solution using a microplate reader. At the same time, according to the size of the absorbance value, the concentration of hydrogen peroxide can be inferred.

[0035] figure 2 For using TMB as chromogenic agent, nano-cobalt trioxide detects hydrogen peroxide diagram.

Embodiment 3

[0037] Nano-cobalt trioxide as a peroxide mimetic enzyme to detect hydrogen peroxide content

[0038]Add 50ml, 1mg / ml cobalt tetroxide solution, 20ml (5mg / ml) ABTS solution to the solution, and then add different amounts of H 2 o 2 , such that H 2 o 2 The final concentration is 0, 10 -9 , 5×10 -9 , 10 -8 , 5×10 -8 , 10 -7 , 5×10 -7 , 10 -6 , 5×10 -6 , 10 -5 , 5×10 -5 , 10 -4 , 5×10 -4 , 10 -3 , 5×10 -3 , 10 -2 (mol / L). Sodium acetate buffer solution (pH=4.0) was added so that the final volume of the solution to be tested was 200ml. Measure the absorbance of the solution using a microplate reader. At the same time, according to the size of the absorbance value, the concentration of hydrogen peroxide can be inferred.

[0039] image 3 For using ABTS as chromogenic agent, nano-cobalt trioxide detects hydrogen peroxide diagram.

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Abstract

The invention relates to application of nanometer cobaltosic oxide (Co3O4) used as a peroxide enzyme mimic for measuring the concentration of hydrogen peroxide; nanometer cobaltosic oxide used as the peroxide enzyme mimic has peroxidase-like activity, and further comprises various sizes of nanometer cobaltosic oxide particles; the particle diameter of nanometer cobaltosic oxide exceeds 9 nm; a cobaltosic oxide nanometer material can be used for catalyzing degradation of a phenolic substance, and has potential for being applied to printing and dyeing wastewater treatment. According to the application provided by the invention, the peroxide enzyme mimic function of nanometer cobaltosic oxide is found for the first time, so that nanometer cobaltosic oxide can be used as the peroxide enzyme mimic for measuring the content of hydrogen peroxide; compared with HRP, nanometer cobaltosic oxide used as a catalyst is higher in stability and reusability, and more efficient in peroxide enzyme mimic catalytic activity; without the presence of hydrogen peroxide, nanometer cobaltosic oxide can also be used for catalyzing color developing agents, and used as an enzyme mimic.

Description

technical field [0001] The present invention relates to a kind of nano cobalt tetroxide (Co 3 o 4 ) as a peroxide mimetic enzyme for the determination of hydrogen peroxide. The invention belongs to the field of nanometer biochemical detection. Background technique [0002] Biological enzyme is a kind of protein with catalytic function. A small amount of it can greatly speed up the reaction; sometimes it can also participate in the reaction, but there is no change before and after the reaction; it has high catalytic efficiency, specific reaction and mild reaction conditions. However, the disadvantages of natural enzymes, such as variability and inactivation, high price, difficult purification, high storage and use costs, make the study of mimic enzymes a top priority. [0003] Since the first discovery of magnetic ferric oxide nanoparticles (Fe 3 o 4 NPs) have peroxidase-like catalytic activity, that is, Fe 3 o 4 NPs can catalyze H 2 o 2 React with the substrate...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 何丹农王萍夏智伟刘婷
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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