113 results about "Enzyme function" patented technology

The invention discloses a method for preparing soil amendment for southern dry and acid soil. It comprises following steps: mixing calcinated shell product of 40- 64% (proportion by weight), calcium magnesium silicate of 15- 30% and ammoniomagnesium phosphate of 10- 28%, getting mixed material; disintegrating mixed material, sifting, and getting soil amendment. The product can improve cluster structure for soil, reduce water loss and fertilizer loss problem in southern dry and acid soil, prevent soil consolidation; it can moderate the pH value for southern dry and scid soil, neutralizing free acid, inactivating the activity of active aluminium, improve microenvironment for root soil and increase microbe diversity in southern sry and acid soil, increase the enzyme functions, improve nutrition supply of magnesium and calcium and other microelement, and provides good basis for high crop productivity.

Disclosed herein are water soluble compositions, such as films, including a mixture of a first water-soluble resin, an enzyme, and an enzyme stabilizer which comprises a functional substrate for the enzyme, methods of making such compositions, and methods of using such compositions, e.g. to make packets containing functional ingredients. The enzymes can include proteases and mixtures of proteases with other enzymes, and the compositions provide good retention of enzyme function following film processing and storage.

An electrochemical biosensor enzyme function sensitive film containing nickel aluminum hydrotalcite nanometer plate and a preparation method belong to the technical field of electrochemical biosensor and its preparation. Wherein, the sensitive film comprises heme proteins, high molecule substances and nickel aluminum hydrotalcite nanometer plates. The preparation method of the sensitive film includes steps below: Hydrotalcite nanometer plate sols are mixed with heme proteins, dipped onto a clean glass carbon electrode surface then and submerged into the high molecule substance PVB solution for static placement after being dried at room temperature to volatilize the solvent, thus forming a layer of enzyme function sensitive film containing nickel aluminum hydrotalcite nanometer plate. The sensitive film of the present invention is suitable for determination of peroxide and has the advantages of simple production technique, lower cost and wide detecting scope, etc.

The invention provides a method for preparing litchi enzymes. According to the method, litchis with white peels are placed in orange juice to be soaked for 3-5 hours in a sealed mode, then anaerobic fermentation and filtration are conducted, and first filter liquor and first pomace are obtained; the first filter liquor is concentrated to 5-10 g/mL, and fermentation continues to be conducted; aerobic fermentation and refiltration are conducted on the first pomace, and second filter liquor and second pomace are obtained; the second pomace is placed in an ultrasonic container for vibration filtration, third filter liquor is obtained, the third filter liquor and the second filter liquor are mixed and then added to the concentrated first filter liquor, a strain IV is added to the mixture, then fermentation continues to be conducted for 5-10 days, and the litchi enzymes are obtained. According to the method for preparing the litchi enzymes, three times of fermentation is carried out, on the basis of anaerobic fermentation, the strain is inoculated for alternate aerobic fermentation, fermentation is conducted on the inoculated strain through the sugar of the inoculated strain, the culture is combined with the intergrowth advantage of beneficial bacterium for rapid fermentation, the fermentation speed is increased, infectious microbes are reduced, mildew is avoided, the activity of enzymes is improved, the number of viable organisms is increased, the enzyme function is improved, and the enzyme sugar content is also lowered.

The invention relates to an application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide concentration measurement. The application has the advantages that the nanometer MnOx and CrOx are discovered to have a peroxide mimic enzyme function for the first time, and can be used for detecting hydrogen peroxide content; the prepared nanometer MnOx and CrOx serving as catalysts have high stability, reusability and efficient peroxide mimic enzyme catalytic activity in comparison to HRP; the condition that without the existence of hydrogen peroxide, the nanometer MnOx and CrOx also can catalyze a color developing agent, and are used as mimic enzymes is discovered; a preparation method is simple in process, high in operability and capable of further meeting production and application needs.

The invention relates to a preparation method of enzyme function sensitive film comprising a methylene blue intercalation manganese dioxide supermolecular structure material and the application thereof in electrochemistry biological sensor; the methylene blue is inserted into manganese dioxide layers by the method of removal and reassembly; an ethanol dispersion liquid is dropped and smeared on the surface of a solid electrode; a homogeneous mixed liquid of a biological compatibility polymer and a biological active substance is dropped and smeared on the surface of the solid electrode after the ethanol dispersion liquid dries naturally; the solid electrode is immersed into a high polymer film material solution after being dry; a solvent on the surface of the solid electrode is volatilized in a refrigerator, and then a layer of a complex enzyme function film comprising the methylene blue intercalation manganese dioxide can be formed on the surface of the solid electrode. The enzyme function sensitive film in the invention is used in the electrochemistry biological sensor and greatly improves the performances of the sensor such as detecting potential, anti-jamming capacity and stability.

This invention describes the coding gene and applications of a nitrobenzene nitro-reducing enzyme, comprising a protein from the following amino acid residue sequences: 1) SEQ ID No. 1 in the sequence table; 2) the amino acid residue sequence in SEQ ID No. 1 is a protein substituted, deleted or added with 1-10 amino acid residues and having the nitrobenzene nitro-reducing enzyme function. The nitrobenzene nitro-reducing enzyme and its coding gene in this invention play an important role in the production of ortho-aminophenols and their derivatives.

The invention discloses a cephalosporin acylase mutant and an encoding gene and an application thereof. The protein provided by the invention is any one of the following 1)-3): 1) protein formed by an amino acid sequence shown as sequence 4 in the sequence table; 2) protein formed by an amino acid sequence shown as sequence 3 in the sequence table; 3) protein which is obtained by substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid residue sequence of sequence 3 or sequence 4 in the sequence table, has the functions of cephalosporin acylase, andis derived from 1). Experiment of the invention shows that the invention provides a wild-type CPC acylase mutant; HPLC results show that the specific activity for CPC of the wild-type CPC acylase mutant of the invention is increased by 6.5 times when compared with that of wild-type enzymes. In one-step conversion, the conversion rate is up to above 98% within 3 hours.

The invention belongs to the field of molecular biology and relates to a detection technology for separation and clone, site-specific mutagenesis, zymoprotein prokaryotic expression, zymoprotein separation and purification and enzyme functions of a branch-point key enzyme gene participating in synthesis of an isoprenoid matter in a wheat mevalonic acid metabolic pathway. The method can provide important technical storage for further performing the gene improvement and modification, constructing an eucaryon gene expression carrier of a farnesyl diphosphate synthase gene, and converting corresponding crops; secondly can be used for obtaining plants with important commercial values in virtue of secondary metabolism, and discussing a relationship of the overexpression of the farnesyl diphosphate synthase gene in a receiver plant and important agronomic traits such as secondary metabolites, crop grain sizes and crop grain weights; and improving the yield of the crops, analyzing structures of introns, exons and promoters, studying functions of the promoters, developing related molecular markers.

The invention discloses a nutrient solution containing small molecule peptide, a mask containing the same and a manufacturing method. The mask is prepared from the following ingredients in parts by weight: the nutrient solution containing the small molecule peptide, water, hydroxyethyl cellulose, xanthan gum, glycerol, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, sodium hyaluronate, erythritol, sclerotium gum, EDTA disodium, arginine, arbutin, ethyl ascorbic acid, tremella extract, radix gentianae extract, camellia flower extract, hydroxylethyl urea, hydrogenated castor oil and pelargonium graveolens flower oil. The nutrient solution contains the small molecule peptide with extremely high biological activity; as a molecular weight of the small molecule peptide is small, the small molecule peptide can directly enter cells in original shapes through membranal permeation, further participates in enzyme synthesis, stimulates enzyme activity and enhances enzyme function; meanwhile, the small molecule peptide can further effectively provide nutrients for the cells and replace and eliminate toxin in the cells. The mask has varieties of effects of removing wrinkle, whitening skin, resisting ageing and the like.

The invention provides a compound of formula I:

wherein A¹, A², A³, R¹, X, Y, and B have any of the values described herein, as well as salts of such compounds, compositions comprising such compounds, and therapeutic methods that comprise the administration of such compounds. The compounds are inhibitors of monoamine oxidase B (MAO-B) enzyme function and are useful for improving cognitive function and for treating psychiatric disorders in animals.

The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg/L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.

The invention uses two genes of RnD6C and RnD6D which have complete reading frames and are from ribes nigrum L. DNA to express the RnD6C and RnD6D in a yeast expression system; the GC-MS analysis shows that protein which is coded by the two genes in the yeast can respectively catalyze the external substrates of linoleic acid (LA) and Alpha-linolenic acid (ALA) to respectively generate Gamma-linolenic acid (GLA) and parinaric acid (SDA), and has the function of Delta<6> fatty acid dehydrogenase. The invention can be applied to that the gene engineering technology is adopted and the expression system of lower eukaryotic cells and the plant expression system are used for producing GLA and SDA.

The invention relates to a saffron, a saffron endophytic fungi GGPPS (geranylgeranyl pyrophosphate synthase) gene, a gene cloning method and application, and belongs to the technical field of gene engineering. The invention discloses two genes including the saffron and GGPPS in metabolic pathway of the saffron endophytic fungi; the two genes have nucleotide sequences shown as SEQ ID NO.4 and SEQ ID NO.3, have the identical open reading frames shown as SEQ ID NO.1 and code amino acid sequences shown as code SEQ ID NO.2. The color genetic complementary reaction verifies that the GGPPS gene coding protein has a typical GGPPS enzyme function; FPP (farnesyl pyrophosphate) and IPP (isopentenyl pyrophosphate) can be catalyzed to be synthesized into the GGPPS (C<20>).

The present invention discloses a Chinese wildrye levan hydrolase, and an encoded gene and an application thereof. The albumen is Chinese wildrye levan hydrolase, which is the protein having one of the following amino acid residue sequence: 1) the amino acid residue sequence of the sequence 1 in the sequence table; and protein that is obtained by substituting and/or deficiency and/or addition of one or more amino acid residue to the amino acid residue sequence of the sequence 1 in the sequence table and has [beta]-2,1 glycosidic bond hydrolase function. The encode gene of the low temperature proof albumen can be transferred to a plant to improve the stress-resistance of the plant. The gene has a significant theoretical and practical significance for the research of the stress-resistance molecular mechanism of plant and the breeding of stress-resistance varieties of plant. The gene provides an economical, fast and effective approach for improving the stress-resistance of crop. The gene has wide applications and market prospect in the agriculture field.

The invention relates to a gene with asparagine synthetase function, encoding protein and application thereof. The gene has one of the following nucleotide sequences: 1, a base sequence shown in SEQ ID NO1; and 2, a DNA sequence which possesses over 95 percent of homology with the nucleotide sequence defined in the sequence 1 of a sequence table and encodes the same biological functional protein. The gene has the function of synthesizing asparagine synthetase, can recover the capability of saccharomyces cerevisiae mutant in synthesizing the asparagine synthetase, and simultaneously indicate the application value of the gene in crop nitrogen high-efficiency utilization aspect.

The invention discloses a quorum-quenching enzyme OLB-26, and a coding gene and application thereof. The protein OLB-26 is a fusion protein disclosed as (a) or (b): (a) protein composed of amino acid sequence disclosed as Sequence 1 in the sequence table; or (b) protein derived from Sequence 1 in the sequence table with quorum-quenching enzyme functions, which is subjected to substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence disclosed as Sequence 1 in the sequence table. The experiment proves that the fusion protein OLB-26 disclosed by the invention has substrate specificity, can degrade long-chain and short-chain N-acylhomoserine lactones, has the specific activity of 7.59 U/mg as a quenching enzyme, is very stable within the pH range of 6.0-8.0, and can maintain more than 80% of enzyme activity; and the quorum-quenching enzyme OLB-26 has favorable heat stability, and the relative enzyme activity at 50 DEG C for 20 minutes is still 100%. The fusion protein OLB-26 disclosed by the invention can be used for preparing a novel biocontrol enzyme preparation.

An integrated system for green tea sorting and de-enzyming comprises a heat exchange unit and a de-enzyming and sorting tower. A cleaning chamber, a de-enzyming chamber, a primary heat exchange chamber, a secondary heat exchange chamber and a sewage pool are sequentially arranged in a cavity formed in a tower body of the de-enzyming and sorting tower from top to bottom, and a spraying device and high-pressure atomizing spray heads are arranged in the cleaning chamber; the de-enzyming chamber is arranged under the cleaning chamber, the side wall of the a tower body of the de-enzyming chamber is provided with three spray pipes which are evenly arranged by surrounding the axis of the tower body at the same height, each spray pipe is obliquely arranged along the interior of the tower, the primary heat exchange chamber is arranged under the de-enzyming chamber, and heat exchange boxes are arranged in the primary heat exchange chamber; the secondary heat exchange chamber is arranged under the primary heat exchange chamber, the sewage pool is arranged under the secondary heat exchange chamber, and an air blower is arranged between a hot air outlet and the heat exchange unit. Accordingly, impurities in tea leaves are fully and completely stripped, tea leaves with the clean surfaces are obtained, and the cleaning and de-enzyming functions are achieved.

The invention discloses application of silybum marianum-derived flavonoid 3 beta-hydroxylase and coenzyme thereof, and belongs to the field of gene and metabolic engineering. According to the invention, SmF3 'H and SmCPR with flavonoid 3'-hydroxylase functions are obtained and verified from silybum marianum, and have the capability of catalyzing naringenin to synthesize eriodictyol. Compared withthe common GhF3 'H and CrCPR, the SmF3' H and SmCPR used in the method have the advantage that the yield of eriodictyol generated by converting naringenin can be increased by 17.0 percent under the same culture condition. On the basis, promoters with different intensities are used for regulating SmF3 'H and SmCPR, the yield of eriodictyol is further increased, the yield of eriodictyol can reach 805.6 mg/L in a 250 mL shake flask, and the yield is increased by 302.8% compared with the reported maximum yield of 200 mg/L. The flavonoid 3 beta-hydroxylase from silybum marianum and coenzyme thereofhave good performance of converting naringenin to synthesize eriodictyol, so that the flavonoid 3 beta-hydroxylase from silybum marianum and coenzyme thereof have wide application prospect.

The invention discloses a delta12-fatty acid dehydrogenase gene separated from mortierella isabellina M6-22, the nucleotide acid sequence is shown in the SEQIDNO:1, the code is an amino acid sequence shown in the SEQIDNO: 2; by constructing a recombinant carrier and expressing in saccharomyces cerevisiae, the expression product has delta12-fatty acid dehydrogenase function and is capable of catalyzing the oleic acid to convert into linoleic acid.