A dry-sheet type serum creatinine detection reagent strip and a preparing method thereof

Abstract

A dry-sheet type serum creatinine detection reagent strip and a preparing method thereof are disclosed and belong to the field of in-vitro diagnosis. A creatinine reacting membrane, a filtering membrane and a diffusion layer in the reagent strip are fixed to a plastic plate in an appropriately overlapping manner. The reagent strip is characterized in that the reacting membrane includes a diluting liquid and a reacting reagent, the diluting liquid includes a buffer liquid, a surfactant, a stabilizer, an adhesive, a coenzyme factor and an aseptic, and the reacting reagent includes creatinine amidohydrolase, creatinase, sarcosine oxidase, peroxidase, 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline. The Trinder reagent is utilized as an indicator probe, an analyte finally generates a colored quinone compound through various enzyme functions, and the creatinine content of a sample is indirectly detected through the coloration degree of the quinone compound. According to the reagent strip and the method, a liquid phase reaction is transferred to a solid carrier, objectives of simple operation and rapid and convenient detection of the creatinine content of the sample are achieved, and the reagent strip and the method are high in sensitivity, good in accuracy and convenient in popularization and application.

Description

technical field [0001] The invention relates to a detection reagent strip for clinical detection of creatinine content in serum, belonging to the technical field of clinical in vitro diagnosis. Cooperating with the reflected light analyzer matched with the dry chemical reagent strip, the accuracy, precision and analysis speed of creatinine detection are improved. Background technique [0002] 1. Creatinine is a product of muscle metabolism. In muscle, creatinine is slowly formed into creatinine through an irreversible non-enzymatic dehydration reaction, and then released into the blood. When it passes through the kidney, it will be filtered out by normal glomeruli. Almost all are excreted with urine. Creatinine includes blood creatinine and urine creatinine. Elevated serum creatinine can better reflect substantial glomerular function damage and is an important indicator for evaluating glomerular filtration function. It is highly valued in clinical practice and is a necessar...

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Problems solved by technology

Although this method is low in cost, it is easily affected by a variety of interfering substances, and the specificity and accuracy of measuring serum creatinine are poor; the other method is sarcosine oxidase, which overcomes the influence of many interfering substances and has strong specificity , the accuracy is high, but there is a defect that it takes a long time, and it is inconvenient to use clinically

[0004] At present, there is a test paper for measuring creatinine content. Its principle is similar to the determination of creatinine by the picric acid method. There is a problem that non-creatinine substances interfere with the measurement, and the reaction needs to be carried out under strong alkali conditions. Very unfavorable for long-term stable storage

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