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33 results about "Creatinase" patented technology

In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction creatine + H₂O ⇌ sarcosine + urea Thus, the two substrates of this enzyme are creatine and H₂O, whereas its two products are sarcosine and urea. The native enzyme was shown to be made up of two subunit monomers via SDS-polyacrylamide gel electrophoresis. The molecular weights of these subunits was estimated to be 47,000 g/mol.

Kit and method for assaying creatinine

The invention relates to a kit and a method for assaying creatinine. The kit includes a first reagent group and a second reagent group. The first reagent group includes creatinase, sarcosine oxidase, chromogen and catalase; the second reagent group includes creatininase, 4-amino antipyrine and peroxidase. The chromogen is selected from N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline or a sodium salt thereof, and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and the method can reduce interference during the detection of the creatinine due to the calcium dobesilate and / or etamsylate that exist in a sample.
Owner:PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1

Creatinine detection reagent

The invention discloses a creatinine detection reagent which is characterized by comprising a diluent and a reaction reagent, wherein the diluent is composed of buffer, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of buffer, creatinase, sarcosine oxidase, peroxidase, N-ethyl-N-(3-propylsulfo)-3-methylaniline, creatininase, 4-aminoantipyrine, preservative and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD

Detection kit for determining content of creatinine in serum by enzymic method

The invention discloses a detection kit for determining the content of creatinine in serum by an enzymic method. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains 3-10g / L of buffering solution with the pH value being 7.5-8.0, 0.2-0.5g / L of EDTA, 0.5-2g / L of N-ethyl-N-(2-hydroxyl-3-sulfopropyl) m-toluidine sodium salt, 1 per mill-5 per mill of a surface active agent, 0.2-1g / L of sarcosine oxidase, 1-5KU / L of ascorbate oxidase, 2-5KU / L of creatinase and 0.5-1g / L of a stabilizing agent; the reagent R2 contains 3-10g / L of buffering solution with the pH value being 7.5-8.0, 0.1-0.5g / L of 4-aminoantipyrine, 0.1-0.4g / L of potassium ferricyanide, 2-8g / L of creatininase amidohydrolase, 1-6KU / L of peroxidase and 0.5-2g / L of a preservative. The detection kit disclosed by the invention is excellent in stability, strong in interference resistance and high in clinical application value.
Owner:上海睿康生物科技有限公司

Detection reagent and test strip for creatinine

The invention discloses a detection reagent and a test strip for creatinine. Each liter of the detection reagent contains 12-18 KU of creatininase, 12-20 KU of creatinase, 9-12 KU of sarcosine oxidase, 15-20 KU of horseradish peroxidase, 5-8 KU of ascorbic acid oxidase, 0.20-0.35 g of 4-aminoantipyrine and 0.20-0.30 g of color developing matter. The detection reagent contains various specific enzymes for specific proportioning, and can achieve the purposes of rapid detection and more accurate detection. The test strip comprises a reaction base layer, a reaction layer, a blood filter layer and a hydrophilic layer which are stacked to form a siphon system, thereby further guaranteeing rapid detection and more accurate detection.
Owner:复星诊断科技(长沙)有限公司

Stable creatinine detection kit and use method thereof

The invention discloses a stable creatinine detection kit and a use method thereof, relates to the field of biochemical detection and aims to provide a creatinine detection kit which is low in toxicity, high in stability and sensitive and accurate in detection and a use method of the creatinine detection kit. The creatinine detection kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from an HEPES buffer, EDTA (ethylenediaminetetraacetic acid), creatinase, sarcosine oxidase, erythromycin, PEG8000, tween 20, TOOS, a compound stabilizer and a proclin preservative; the reagent 2 is prepared from the HEPES buffer, catalase, creatininase, 4-aminoantipyrine, NPEO, potassium ferricyanide, sodium chloride, ethylene glycol and other substances. The kit has good compatibility, better stability and low toxicity and can resist interference of bilirubin and metal ions.
Owner:WHITMAN BIOTECH NANJING

Creatinine assay kit and application thereof

The invention relates to a creatinine assay kit and application thereof, and belongs to the technical field of biological detection. The creatinine assay kit is composed of a reagent R1 and a reagentR2; the reagent R1 is prepared from creatinase, catalase, sarcosine oxidase, 4-amino-antipyrine, an inhibitor and a buffer solution; and the reagent R2 is prepared from creatinase, catalase, a color developing agent, ascorbic acid oxidase, an inhibitor and a buffer solution. According to the creatinine assay kit, assaying is conducted through a sarcosine oxidase method, and the creatinine assay kit has the advantages of wide linear range, good stability and the like.
Owner:浙江夸克生物科技有限公司

Creatininase method detection kit

The invention discloses a creatininase method detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution, creatinase, sarcosine oxidase,ascorbic acid oxidase, bilirubin oxidase, TOOS, peroxidase, a stabilizer, and a surfactant; the reagent R2 is prepared from a buffer solution, 4-aminoantipyrine, creatininase, a stabilizer, a surfactant, and 0.1 to 10g / L anti-bacterial preservative. The creatininase method detection kit has the advantages of lowering the toxicity effectively, lowering the environment pollution, preventing the drugtolerance of bacteria, preventing super bacteria from generating and lowering the human health risk, along with high stability, and can be applied to creatinine detection of chronic disease management.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD

Creatinine content detecting reagent and kit, and manufacturing and using methods of kit

The invention relates to a creatinine content detecting reagent and kit and manufacturing and using methods of the kit. The creatinine content detecting reagent comprises creatininase, creatinase, creatine oxidase and horseradish peroxidase; and a fluorescent dye, i.e., 5(6)-carboxyl-2,7-dichlordihydro fluorescein ester dipivalate comprises a dye bottom layer, a protease fixing layer and a top layer protecting layer. The using method has high selectivity; a protease has high specificity to reactants; the creatininase can be only used for catalyzing the hydrolysis reaction of creatinine, and does not have catalysis activity on other reaction substrates; three enzymes with high specificity are related to the detection process, so that the selectivity of the method is increased exponentially; the response fed by the kit is fluorescent response; and compared with certain methods for detecting color variations, the invention has the advantages of high sensitivity and interference resistance under the action of the inherent characteristics of fluorescent response.
Owner:TIANJIN HEOWNS BIOCHEM TECH

Creatinine detection kit and use method thereof

The invention provides a creatinine detection kit and a use method thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises creatinase, sarcosine oxidase, peroxidase,ascorbic acid oxidase, 2, 4, 6-tribromo-3-hydroxybenzoic acid and 3, 5-dichloro-2-hydroxybenzenesulfonic acid; and the reagent R2 comprises creatinase, peroxidase, potassium ferrocyanide and 4-aminoantipyrine. The creatinine detection kit provided by the invention can resist interference of etamsylate with the concentration of 300mg / L (the highest blood concentration) or below in a to-be-detectedsample, has a wider linear range (0-6000 millimoles / L), is applicable to detection of samples with high creatinine content and can correctly evaluate renal functions of surgical patients.
Owner:LANZHOU BAIYUAN GENE TECH

Creatinase mutant with improved heat stability

The invention discloses a creatinase mutant with improved heat stability, belonging to the field of enzyme engineering. A nucleotide sequence for coding the creatinase mutant is as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4 or SEQ ID No. 5. Compared with the half-life period of wild type creatinase, the half-life period of the creatinase mutant is respectively increased by 6.9, 3.7, 3.4, 1.6 and 1.2 times, so that the creatinase mutant is more suitable for industrial application.
Owner:JIANGNAN UNIV

Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof

The invention relates to a creatinine kit capable of eliminating negative deviation interference in a creatinine determination caused by calcium dobesilate and etamsylate drugs in serum and a preparation method thereof. Key points of a technical scheme of the invention are that the creatinine kit includes a reagent R1 and a reagent R2, wherein the reagent R1 includes buffer, creatinase, sarcosineoxidase, ascorbic acid oxidase, peroxidase or catalase, serum albumin, nonionic surfactant, preservative, Trinder's reagent A, laccase or metalic acid salt; and the reagent R2 includes buffer, serum albumin, creatinine enzyme, preservative and Trinder's reagent B. The invention overcomes a defect of inaccurate creatinine determination due to the presence of the negative deviation caused by calciumdobesilate and etamsylat in serum samples when the clinical tests of creatinine determination are being carried out in various hospitals, and the invention is suitable for the detection of serum creatinine by an automatic biochemical analyzer.
Owner:ZHONGSHAN BGH BIOTECH CO LTD

Reagent combination, reagent or kit for measuring creatinine content

The invention relates to the technical field of biology, and particularly relates to a reagent combination, a reagent or a kit for measuring creatinine content. The reagent combination comprises a reagent group 1 and a reagent group 2; the reagent group 1 comprises creatinase, sarcosine oxidase, ascorbic acid oxidase, chromogen and catalase; the reagent group 2 comprises creatinase, 4-aminoantipyrine and peroxidase; and the chromogen is selected from N-(2-hydroxy-3-sulfopropyl)-3, 5-dimethoxyaniline or sodium salt thereof and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-5-dimethoxyaniline. An inhibitor is added into the measured reagent, so that generation of neocreatine and creatinine in the process of measuring is blocked, and interference existing in a sample in creatinine detection is lowered.
Owner:柏定生物工程(北京)有限公司

Kit and method for measuring creatinine

The invention discloses a kit and method for measuring creatinine. The kit comprises a first reagent, and the first reagent is prepared from monopotassium phosphate, dipotassium phosphate, ethylenediamine tetraacetic acid, Brij 35, Tween 80, ascorbic acid oxidase, creatinase, sarcosine oxidase, TOOS, peroxidase, potassium ferrocyanide, gentamicin sulphate and Tween 30. The method comprises the following steps: (1) the first reagent and a second reagent are dissolved according to the raw material components and content; (2) the dissolved first reagent is added into a to-be-measured sample; (3)the dissolved second reagent is added; and (4) the absorptivity of a solution under the wavelength of 546 nm is measured. According to the kit and method for measuring the creatinine, the precision and accuracy are high, and stability is achieved.
Owner:SHANGHAI HIGH TRACK MEDICAL EQUIP CO LTD

Detection kit for measuring creatinine through enzyme method and using method thereof

The invention relates to the field of biochemical detection, in particular to a detection kit for measuring creatinine through an enzyme method and a using method thereof. The kit comprises various reagents of sarcosine oxidase, creatinase, catalase, a buffer solution and the like prepared by using a genetically engineered bacterium GLYH18 prepared by a LYH18 bacterial strain with the preservationnumber of CGMCC No.17306, the sample is mixed with the reagents, and then, the final reactant is placed under a biochemical analyzer to detect the change condition of absorbance so as to measure thecontent of creatinine. The preparation method of the sarcosine oxidase in the kit is simple and low in cost, the content of creatinine in the sample can be quickly and accurately measured by the kit with the enzyme added, the kit can be kept stable at room temperature, which facilitates practical application and better facilitates industrial production.
Owner:DALIAN UNIV

Method for improving heat stability of creatinase

The invention discloses a method for improving the heat stability of creatinase, and belongs to the fields of genetic engineering and enzyme engineering. According to the method, a mutant is obtained by carrying out amino-acid substitution and mutation on the basis of the creatinase from a Pseudomonas putida source. By comparing the mutant H125C / L130C provided by the invention with wild-type creatinase, the Tm value is increased by 8.2 DEG C, and the heat stability is obviously improved. Through the method provided by the invention, the heat stability of the creatinase is effectively improved.
Owner:JIANGNAN UNIV

Serum creatinine detection reagent ball and serum creatinine detection chip

The invention relates to the technical field of serum creatinine determination, and in particular relates to a serum creatinine detection reagent ball and a serum creatinine detection chip. According to the serum creatinine detection reagent ball and the serum creatinine detection chip containing the serum creatinine detection reagent ball, interference of vitamin C in a detection sample can be eliminated through ascorbic acid oxidase, and interference of bilirubin can be eliminated by taking a bilirubin interference removing agent as an oxidizing agent; interference of endogenous creatine, sarcosine and peroxide existing in a detection sample is eliminated through creatinase, sarcosine oxidase and peroxidase respectively. The serum creatinine detection reagent ball and the serum creatinine detection chip provided by the invention have relatively strong anti-interference capability on original interferents in serum and drug interferents taken by patients, and the precision of a detection result is high.
Owner:GENRUI BIOTECH INC

Creatinine detection reagent capable of resisting interference of medicines such as calcium dobesilate

The invention discloses a creatinine detection reagent capable of resisting interference of medicines such as calcium dobesilate. The creatinine detection reagent comprises a first reagent and a second reagent, wherein the first reagent comprises 3-(N-morpholinyl)propanesulfonic acid, TOOS, gentamicin, sarcosine oxidase, creatinase, ascorbic acid oxidase and catalase, and the second reagent comprises 3-(N-morpholinyl)propanesulfonic acid, 4-AAP, creatininase amidohydrolase, peroxidase, preservative and potassium ferrocyanide; the first reagent and / or the second reagent further comprise / comprises piperidine oxygen radicals. According to the creatinine detection reagent provided by the invention, the interference effect of medicines such as calcium dobesilate can be reduced, the accuracy ofcreatinine detection in clinical detection is ensured, and a creatinine detection method with a wide linear range, strong medicine interference resistance and high accuracy is realized.
Owner:WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD

Creatinine content determination method and creatinine diagnosis kit

The invention relates to a method for determining the activity of creatinine, and also the reagent kit for creatinine diagnosis. The reagent kit comprises cushioning solution, coenzyme, creatinine enzyme, creatinase, creatine oxidase, peroxydase, aldehyde dehydrogenase, deacidized chromogen combination, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the creatinine content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.
Owner:王尔中

Electrode and test paper for detecting creatinine by electrochemical method and preparation method of electrode and test paper

The invention provides an electrode for detecting creatinine by an electrochemical method. The electrode comprises an electrode material base layer, the outer surface of the electrode material base layer is covered with an electron mediator enhancement layer; an enzyme composition capable of reacting with creatinine to generate hydrogen peroxide is fixed on the outer surface of the electron mediator enhancement layer; the electrode material base layer is composed of carbon paste or graphite containing Prussian blue; the enzyme composition is prepared from creatininase, creatinase and sarcosine oxidase according to the ratio of (3.75-6.25) to (1.25-2) to (0.75-1.25). The invention also provides test paper and a biosensor based on the electrode. The invention also provides a preparation method for preparing the electrode and the test paper, and a method for detecting the creatinine level by using the biosensor. The test paper provided by the invention can be used in various portable and rapid small electrochemical detection devices, and can realize rapid and convenient clinical detection or home monitoring of blood and urine creatinine.
Owner:PEKING UNIV FIRST HOSPITAL +1

Creatinine content determination method and creatinine diagnosis kit

The invention relates to a method for determining the activity of creatinine, and also the reagent kit for creatinine diagnosis. The reagent kit comprises cushioning solution, pyroracemic acid, phosphoenolpyruvate phosphatase, creatinine enzyme, creatinase, urease, phosphoenolpyruvate carboxylase, pyruvic oxidase, peroxydase, stabilizer, and deacidized chromogen combination. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the creatinine content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.
Owner:王尔中

Creatinine content detecting reagent and kit, and manufacturing and using methods of kit

The invention relates to a creatinine content detecting reagent and kit and manufacturing and using methods of the kit. The creatinine content detecting reagent comprises creatininase, creatinase, creatine oxidase and horseradish peroxidase; and a fluorescent dye, i.e., 5(6)-carboxyl-2,7-dichlordihydro fluorescein ester dipivalate comprises a dye bottom layer, a protease fixing layer and a top layer protecting layer. The using method has high selectivity; a protease has high specificity to reactants; the creatininase can be only used for catalyzing the hydrolysis reaction of creatinine, and does not have catalysis activity on other reaction substrates; three enzymes with high specificity are related to the detection process, so that the selectivity of the method is increased exponentially; the response fed by the kit is fluorescent response; and compared with certain methods for detecting color variations, the invention has the advantages of high sensitivity and interference resistance under the action of the inherent characteristics of fluorescent response.
Owner:TIANJIN HEOWNS BIOCHEM TECH

A creatine hydrolase mutant with improved thermostability

The invention discloses a creatinase mutant with increased thermal stability, and belongs to the field of enzyme engineering. The mutant is obtained by mutating a 368th amino acid of a creatinase amino acid sequence. Creatinase roots in escherichia coli. The mutant is a mutant V368M obtained by mutating a 368th valine into methionine. An amino acid in creatinase protein rooting in the escherichia coli is subjected to site-specific mutagenesis, so that thermal stability of the creatinase is improved. Compared with the existing mutant V368M, the creatinase mutant with increased thermal stability has the advantages that half-life is prolonged for 5 times, and Tm value is increased by 3 DEG C.
Owner:JIANGNAN UNIV

A kind of improved highly active thermostable creatine hydrolase and its application

The invention relates to a mutant of creatinase. The specific activity and the heat stability of the creatinase are improved. Compared with wild-type creatinase, the mutant is more suitable for being applied to industrialization and has excellent market application prospects and economic values.
Owner:湖南艾科瑞生物工程有限公司

Method for improving pH stability of creatinase

The invention discloses a method for improving pH stability of creatinase, and belongs to the field of enzyme engineering. According to the method, creatinase of which the pH stability is improved and the kcat / Km is only reduced by 24 percent is obtained by modifying free creatinase homodimer through polylysine. The method is mild in conditions and easy to operate, and is more suitable for industrial application; convenience is provided for the application of the creatinase to the fields, such as medical diagnosis. Moreover, the method provided by the invention also provides a feasible way for stability transformation of other multi-subunit enzymes.
Owner:JIANGNAN UNIV

creatinine test strips

The invention discloses a detection reagent and a test strip for creatinine. Each liter of the detection reagent contains 12-18 KU of creatininase, 12-20 KU of creatinase, 9-12 KU of sarcosine oxidase, 15-20 KU of horseradish peroxidase, 5-8 KU of ascorbic acid oxidase, 0.20-0.35 g of 4-aminoantipyrine and 0.20-0.30 g of color developing matter. The detection reagent contains various specific enzymes for specific proportioning, and can achieve the purposes of rapid detection and more accurate detection. The test strip comprises a reaction base layer, a reaction layer, a blood filter layer and a hydrophilic layer which are stacked to form a siphon system, thereby further guaranteeing rapid detection and more accurate detection.
Owner:复星诊断科技(长沙)有限公司

Modified creatinase

The invention relates to a mutant polypeptide having creatine amidinohydrolase activity, where the polypeptide both retains thermostable activity in the presence of reagents that modify thiol groups. The invention further relates to methods for producing the mutant polypeptide; and to a sensor comprising the mutant polypeptide for use in the measurement of creatinine in samples of physiological fluids. Additionally, the invention teaches how to use the mutant polypeptide to enhance the life-time of a creatinine sensor, and a method for producing the sensor.
Owner:RADIOMETER AS
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