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Kits and methods for measuring creatinine

A technology of creatinine and usage, applied in the field of medical testing, can solve problems such as incorrect assessment of renal function, delayed treatment, and affecting patient prognosis

Active Publication Date: 2018-08-31
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Negative interference of calcium dobesilate and acetamine on the detection of creatinine by the sarcosine oxidase method caused great inconvenience to patients who were administered these two drugs
For example, for diabetic patients, renal insufficiency is often accompanied, and the negative interference of administered calcium dobesilate to creatinine oxidase method may lead to incorrect assessment of renal function in diabetic patients, masking and delaying the condition
For patients undergoing surgery, the negative interference of the sarcosine oxidase method to detect creatinine may make it impossible to correctly evaluate their renal function, cause postoperative complications, and affect the prognosis of patients.

Method used

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  • Kits and methods for measuring creatinine
  • Kits and methods for measuring creatinine
  • Kits and methods for measuring creatinine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of creatinine assay kit

[0046] All the components shown in the following Table 1 and the following Table 2 were mixed at the concentrations shown below to prepare reagent sets 1 and 2.

[0047] Table 1: Reagent Set 1

[0048]

[0049] Table 2: Reagent Set 2

[0050]

[0051]

Embodiment 2

[0052] Embodiment 2: the preparation of creatinine assay kit

[0053] All the components shown in Table 3 below and Table 4 below were mixed at the concentrations shown below to prepare reagent sets 1 and 2.

[0054] Table 3: Reagent Set 1

[0055]

[0056] Table 4: Reagent Set 2

[0057]

Embodiment 3

[0058] Embodiment 3: creatinine detection method and repeatability test

[0059] The fresh mixed serum samples without hemolysis, fat turbidity and jaundice were collected from 3 healthy persons. The reagents were calibrated with the creatinine calibrator (concentration: 352.8 μmol / L batch number: 0715031) of Sichuan Mike Biotechnology Co., Ltd. Through a fully automatic biochemical instrument (Hitachi 7170), mix the sample and calibrator with the reagent group 1 in Example 1 and Example 2, and react at 37°C for 5 minutes to obtain mixture 1; Measure the absorbance A1 of the mixture, then add the reagent group 2 in Example 1 and Example 2 to the mixture, and react at 37° C. for 5 minutes to obtain a mixture 2, and then measure the absorbance A2 of the mixture 2 as described above; The creatinine concentration was calculated according to the following formula. The amount of sample used is 4 μl, the amount of reagent used in reagent group 1 is 180 μl, and the amount used in re...

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Abstract

The invention relates to a kit and a method for assaying creatinine. The kit includes a first reagent group and a second reagent group. The first reagent group includes creatinase, sarcosine oxidase, chromogen and catalase; the second reagent group includes creatininase, 4-amino antipyrine and peroxidase. The chromogen is selected from N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline or a sodium salt thereof, and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and the method can reduce interference during the detection of the creatinine due to the calcium dobesilate and / or etamsylate that exist in a sample.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, the present invention relates to a kit and method for detecting creatinine. Background technique [0002] Creatinine is a polar organic nitrogen compound with a relatively small molecular weight (Mw=113.1). The sources of serum creatinine are divided into exogenous and endogenous sources. Exogenous creatinine is the product of meat food metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. When the intake of meat food is stable and the body's muscle metabolism is basically unchanged, the production of creatinine is constant. Creatinine can be filtered through the glomerulus and is rarely absorbed in the renal tubules. Almost all creatinine produced in the body is excreted with urine every day, and is generally not affected by urine output. Clinically, blood creatinine concentration is used to reflect renal function such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N33/70C12Q1/68
Inventor 龙腾镶吴昌英邱玲国秀芝
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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