52 results about "Lactate oxidase" patented technology

The invention discloses a gold-silver alloy nanoprobe based on double-signal cooperative detection, a preparation method and application thereof, and belongs to the technical field of sensing. Then mixing the gold nanorod, a Raman report molecule p-methylthiobenzonitrile and a chiral stimulating factor L-cysteine, and jointly incubating at room temperature to obtain a mixed solution; sequentially adding silver nitrate, chloroauric acid tetrahydrate and ascorbic acid into the mixed solution, and magnetically stirring; and placing the mixed solution under a constant temperature condition to react for a certain time, so that the gold-silver alloy shell fully grows, and finally obtaining the spiral structure alloy nanoprobe with Raman signals and CD signals. The probe realizes the dual detection functions of Raman signals and circular dichroism signals, and can detect the lactic acid concentration with high sensitivity and high selectivity by combining the specific catalytic action of the lactate oxidase, and meanwhile, the Raman signals and CD signals of the silent zone can further expand the application range of the probe.

The invention discloses a loose macroporous iron-based nano catalytic compound for inducing tumor para-apoptosis and a preparation method and application thereof.The nano catalytic compound (TF-Fe at LC) is prepared by taking a porous Fe3O4 nano cluster composed of single crystals as a carrier, modifying the surface of the carrier with an acid-responsive tannic acid-iron (TA-Fe < 3 + >) network and co-loading lactate oxidase (LOD) and a mitochondrial uncoupling agent CCCP in the carrier. The TF-Fe-coated LC constructed by the invention does not need additional chemotherapeutic drugs, and tumor cells are induced to generate a large amount of active oxygen through catalytic cascade and mitochondrial oxidative stress, so that parapoptosis of the tumor cells is triggered; the parapoptotic cells can play a role of a tumor vaccine, induce other tumor cells to generate immunogenic death, and activate an organism immune system to recognize and kill tumors. In addition, the nano catalytic system can be combined with drugs and immunosuppressants to realize a synergistic anti-tumor effect.

The present invention provides a flexible lactate and pH dual-function sensor and its preparation method and application. The preparation method comprises the following steps: (1) coating a mixed slurry of hydroxyethyl cellulose and sodium lignin sulfonate on a polyethersulfone film, and using a CO2 laser to engrave the polyethersulfone film to obtain an ‑O‑S / PES‑LIG electrode, wherein the ‑O‑S / PES‑LIG electrode comprises a first functional region and a second functional region. (2) modifying polyaniline in the first functional region and modifying an enzyme immobilization layer composed of Prussian blue and lactate oxidase in the second functional region; (3) arranging a reference electrode in the peripheral area of ​​the first functional region and the second functional region of the ‑O‑S / PES‑LIG electrode, and drop-coating a conductive slurry. The sensor of the present invention has the characteristics of good flexibility and adaptability, controllable enzyme activity, and high structural integration, and can achieve synchronous and accurate detection of key metabolic indicators such as lactate and pH.

A method for preparing a polymer nanoenzyme for regulating the tumor immunosuppressive microenvironment belongs to the field of nanomedicine preparation. The present invention designs a polymer nanocarrier that responds to the microacidic environment of the tumor and encapsulates lactate oxidase for selectively consuming lactic acid in tumor tissues to improve the tumor immunosuppressive microenvironment and enhance the tumor immunotherapy effect. The nanodrug delivery platform greatly improves the circulation time and stability of lactate oxidase by encapsulating lactate oxidase into a polymer nanocarrier. In addition, by specifically changing the properties such as permeability in tumor tissues, the nanocarrier can significantly reduce the toxic side effects of lactate oxidase. The drug lactate oxidase of the present invention catalyzes the oxidation of lactic acid into pyruvic acid and hydrogen peroxide, thereby improving the tumor immunosuppressive microenvironment, enhancing the immunotherapy effect, etc.

The invention discloses a lactate oxidase portable mobile phone sensing method based on a composite MXene thin-layer material and an application of the lactate oxidase portable mobile phone sensing method. Firstly, a Cu0. 9Zn0. 1S (at) MXene thin-layer material is synthesized and can catalyze H2O2 into hydroxyl free radicals, the generated hydroxyl free radicals react with a colorless 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) solution to generate blue oxidation-state 3, 3 ', 5, 5'-tetramethyl benzidine (oxTMB), the oxTMB can have an obvious ultraviolet absorption peak at 652nm, and a novel visual analysis method is constructed by taking the peak as signal output; besides, lactate oxidase can catalyze lactic acid to generate pyruvic acid and H2O2, a new method is used for LA or LOX analysis by taking H2O2 as a relation, H2O2 generated by the reaction can participate in the oxidation reaction of TMB, an obvious ultraviolet absorption peak can appear at 652nm, and the inhibitor Fe (III) can inhibit the reaction. On the basis, a portable mobile phone sensing method which is rapid, simple and low in cost is constructed, and the method is successfully applied to sensitive detection of LOX in HEK293S cells and has scientific significance.

The invention discloses a lactic acid-responsive nano composite material for antitumor chemotherapy-immunotherapy. The structure of the nano composite material is a nano structure formed by compounding PEGylated viral vaccine protein, lactate oxidase and hollow manganese dioxide loaded with a hypoxia-sensitive chemotherapeutic drug; the nano composite material provided by the invention can simultaneously induce a tumor to release a large amount of antigens, promote an antigen cell presentation process and improve the tolerance of the tumor to immunotherapy, can efficiently activate natural immune response of a tumor part, realizes a tumor inhibition effect, can regulate and control natural immunity of the tumor from multiple aspects, and has a wide application prospect. And safe and efficient anti-tumor chemotherapy-immunotherapy is realized.

The present invention discloses a lactic acid detection kit and a preparation method thereof, which belongs to the technical field of detection reagents. The kit comprises 3 mL of R1 reagent, 1 mL of R2 reagent and 1 mL of standard product. The R1 reagent contains bovine serum albumin, nanosilver-trehalose complex and PEG4000. The bovine serum albumin can prevent enzyme decomposition and nonspecific adsorption, chelate a small amount of dissociated Ag, and + , preventing competition with H2O2 for the binding sites of horseradish peroxidase and ensuring the stable generation of quinoneimine; PEG4000 plays a dispersing role, assisting the nanosilver-trehalose complex to form a protective film on the surface of lactate oxidase and horseradish peroxidase, protecting the protein structure of the two enzymes, and can capture electrons from the surrounding environment, increasing the electron transfer efficiency of the active centers of the enzymes during the catalytic process of the two enzymes, thereby further increasing the activity of the two enzymes and improving the sensitivity and accuracy of the kit.

Disclosed herein is directed to a lactate oxidase variant that exhibits reduced affinity to lactate as compared to a wild-type lactate oxidase. The lactate oxidase variant has one or more amino acid substitutions occurring at positions 95, 96, and / or 175 of the wild-type amino acid sequence. Also disclosed herein is a method for detecting and quantifying lactate in various liquid samples by using the present lactate oxidase variant. The method mainly includes steps of contacting the liquid sample with said lactate oxidase variant; measuring a current generated by the reaction between the lactate oxidase variant and the lactate in the liquid sample; and determining the concentration of lactate in the liquid sample by interpolating or extrapolating the current with that of a control sample having a known concentration of lactate.

The invention relates to the technical field of nano biological medicines, and discloses a photo-thermal-enzyme catalysis nano-particle as well as a preparation method and application thereof. The photo-thermal-enzyme catalysis nano-particles are constructed by crosslinking water-soluble self-doped polyaniline nano-particles, human serum albumin, lactate oxidase and collagenase through a thioacetal linker capable of being split by active oxygen. Collagenase and lactate oxidase (LOx) are integrated into the same nanoparticle system through a thioacetal (TK) linker, the TK linker is split under the action of tumor microenvironment active oxygen (ROS) and H2O2 generated by catalysis of the LOx, the activity of the two enzymes is synchronously activated, a collagen matrix (physical barrier) is degraded, lactic acid (chemical barrier) is removed, the tumor microenvironment is efficiently remodeled, and the tumor microenvironment can be effectively inhibited. The problems that the regulation effect of a single barrier is limited, and the tumor microenvironment is difficult to improve are solved.

The application discloses an iron modified ZIF8 nanoparticle encapsulating an antitumor drug and a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method of the nanoparticle comprises the following steps: firstly, preparing a zinc hydroxide loaded antitumor drug, then adding 2-methyl imidazole to obtain a ZIF8 nanoparticle encapsulating the antitumor drug, and finally adding Fe 2+ A methanol solution of a salt is reacted to obtain the iron modified ZIF8 nanoparticle encapsulating the antitumor drug. Through process improvement, the application loads an antitumor chemotherapy drug in the ZIF8 material, so that the ZIF8 material has good acid responsive drug release capacity. Through iron element modification, the nanoparticle is endowed with peroxidase activity, so that the nanoparticle can realize acid and hypoxia responsive chemotherapy and chemical kinetics therapy. The nanoparticle and microorganisms capable of producing lactate oxidase are combined to show super strong non-external field responsive precise tumor treatment capacity.

The invention relates to a lactate oxidase modified layered double hydroxide responsive nano-drug as well as preparation and application thereof. The composite material comprises a copper ion doped LDH (layered double hydroxide) nano-material, a drug and lactate oxidase. The nano-drug has the effects of regulating lactic acid metabolism and oxidation-reduction metabolism of tumors, increasing secretion of immune factors, enhancing chemical kinetics therapy and realizing immunotherapy and chemical kinetics combined therapy, and a new thought is provided for constructing the safe, efficient and multifunctional nano-drug.

This invention provides a method for preparing and applying a nanosystem for regulating the osteogenic microenvironment. The nanosystem comprises catalase, lactate oxidase, and a strontium-containing mineralized nanolayer. Experiments demonstrate that this nanosystem can effectively treat bone aging-related diseases, and that catalase and lactate oxidase have a synergistic effect. This invention provides a novel treatment strategy for bone aging-related diseases and has broad prospects for clinical translation.

The invention discloses a sweat detection device based on a one-way drainage composite paper-based fiber membrane and a preparation method, and relates to the technical field of sweat detection. The preparation method comprises the following steps: dissolving SEBS (Styrene-Ethylene-Butylene-Styrene), adding a LiCl aqueous solution to prepare an electrospinning solution, and carrying out electrostatic spinning on the hydrophilic paper-based layer to prepare a composite paper-based fiber membrane; writing a microfluidic channel on the hydrophilic side of the composite paper-based fiber membrane; adding a bromocresol green aqueous solution into the microfluidic channel, and drying to obtain a pH detection module; adding a mixed solution of ferric chloride and potassium ferricyanide into a micro-fluidic channel, drying, adding a mixed solution of chitosan and sodium alginate, adding lactate oxidase or glucose oxidase, drying, and adding a color developing agent to respectively obtain a lactic acid detection module and a glucose detection module; the detection device is simple in structure, convenient to operate, not prone to layering and damage, capable of achieving colorimetric detection of pH, lactic acid and glucose at the same time without crosstalk, and has the advantages of being high in detection accuracy and good in color development effect.

The present invention relates to an electrochemical sensor for multiple detection, a preparation method thereof, and an application thereof, and belongs to the field of biological detection technology. The present invention provides an electrochemical sensor for multiple detection, wherein the reference electrode, the working electrode, and the counter electrode of the sensor are sequentially modified with a GOD-HRP-CS-MB layer, a LOD-HRP-FMN layer, and a COD-CEH-HRP-β-CD layer from bottom to top, wherein the components of the GOD-HRP-CS-MB layer include horseradish peroxidase, methylene blue, glucose oxidase, and chitosan, the components of the LOD-HRP-FMN layer include lactate oxidase and flavin mononucleotide, and the components of the COD-CEH-HRP-β-CD layer include β-cyclodextrin, ferrocene, cholesterol oxidase, horseradish peroxidase, and cholesterol esterase. The sensor can simultaneously measure the concentrations of glucose, lactate, and cholesterol in a sample to be tested.

The present invention combines membrane separation technology with biosensor technology to develop a blood separation sensor membrane that can simultaneously achieve blood separation and physiological indicator detection. A membrane tube with an Fe3O4 sensing layer is immersed in a sulfuric acid mixture containing aniline and ammonium persulfate for low-temperature oxidative polymerization, followed by washing and vacuum drying. A lactate oxidase solution and a glutamate oxidase solution are sequentially deposited on the inner surface and pores of the blood separation sensor membrane via vacuum filtration to obtain a blood separation sensor membrane. The separation membrane layer accurately intercepts blood cells while allowing serum to pass through without loss. The octahedral Fe3O4 sensing nanocrystals with high catalytic activity in the inner pores specifically respond to liver function indicators, generating current signals. This enables real-time screening and dynamic monitoring of the biological components of liver function indicators. The preparation process of this separation sensor membrane is simple and controllable, making it easy to implement large-scale and commercial production, thus resolving the current technical bottleneck in real-time dynamic monitoring of liver function in clinical practice.

The invention relates to the technical field of novel biological materials, and particularly discloses an active oxygen and lactic acid dual-driven chemotactic nano motor and a preparation method thereof. Comprising lactate oxidase and selenium nanoparticles, the selenium nano-particles are formed by ascorbic acid and sodium selenite through a chemical reduction method, and the surfaces of the selenium nano-particles are modified with polysaccharide for stabilizing the selenium nano-particles; the lactate oxidase is loaded on the selenium nano-particles to form the nano-motor. Lactic acid is specifically consumed by loading lactic acid oxidase on the surface of the selenium nano-motor, due to high selectivity, the nano-motor can perform directional chemotactic movement towards a lactic acid high expression site, and the excellent oxidation resistance of selenium nano-particles can remove active oxygen and avoid oxidative stress damage, so that the lactic acid and the active oxygen are consumed at the same time.

A lactate oxidase nanocapsule and a preparation method thereof belong to the field of biomedical technology. The present invention uses poly (ethylene glycol) methyl ether acrylate, 2-methacryloyloxyethyl phosphorylcholine, N-(3-aminopropyl) methacrylamide and acrylamide as monomers, N, N'-methylenebisacrylamide, dimethacrylate glyceride and triblock polylactic acid polyethylene glycol dimethacrylate as cross-linking agents, and utilizes free radical polymerization to synthesize a nanometer-thick polymer shell in situ on the surface of lactate oxidase to construct lactate oxidase nanocapsules. Lactate oxidase nanocapsules can eliminate lactic acid and release immunostimulants, eliminate immunosuppression, and can be used in combination with immune checkpoint blockers to improve the immune efficacy of immune checkpoint blockers.

This invention discloses an engineered *E. coli* strain that secretes lactate oxidase under hypoxia-induced conditions and its applications, relating to the field of biomedical technology. The engineered *E. coli* strain provided by this invention can responsively synthesize lactate oxidase in vitro and under culture medium conditions when exposed to a hypoxic environment. Lactate oxidase decomposes localized lactic acid to produce hydrogen peroxide, thereby killing cells and local bacteria. This process, while treating local symptoms, also kills the *E. coli* vector during culture, reducing the risk of exogenous infection caused by this engineered *E. coli* strain, making it a relatively safe therapeutic strain overall.

The invention discloses a preparation method and application of anti-tumor nano-particles based on lactate oxidase and Prussian blue. The nano-particles are hollow Prussian blue nano-particles (PBNPs), the surfaces of the hollow Prussian blue nano-particles (PBNPs) are modified with CXCR1 targeting polypeptide, and the surfaces of the hollow Prussian blue nano-particles (PBNPs) are internally loaded with lactate oxidase (LOD); the amino acid sequence of the CXCR1 targeting polypeptide is as follows: SAKELRCQCIKTYSKPFHPK, and the amino acid sequence of the CXCR1 targeting polypeptide is as follows: Comprising the following steps: S1, synthesizing through a polypeptide synthesizer, and grafting hydroxyl in the rhodamine fluorescent dye to amino of initial serine for tracing. By grafting the CXCR1 targeting polypeptide and loading the LOD catalytic enzyme, the nano catalysis-photo-thermal material (CXCR1-LOD at PBNPs) with targeting activity and biological enzyme activity can be successfully prepared. Through further in-vivo and in-vitro experimental studies, the anti-tumor effect and the molecular mechanism of the CXCR1-LOD coated PBNPs nano material can be fully confirmed. The material combines targeting property, catalytic property and photo-thermal property, and is a further improvement on early-stage research, so that a new strategy and a new method are provided for treatment of osteosarcoma.

To provide L-lactate oxidase with suppressed activity loss at acidic pH as well as applications therefor.SOLUTION: An enzyme electrode includes: a polypeptide that is composed of a specific amino acid sequence; or a polypeptide that is composed of an amino acid sequence having at least 80% identity with an amino acid sequence represented by any of the specific amino acid sequences and exhibits L-lactate oxidation activity.SELECTED DRAWING: None

Disclosed herein is directed to a lactate oxidase variant that exhibits reduced affinity to lactate as compared to a wild-type lactate oxidase. The lactate oxidase variant has one or more amino acid substitutions occurring at positions 95, 96, and / or 175 of the wild-type amino acid sequence. Also disclosed herein is a method for detecting and quantifying lactate in various liquid samples by using the present lactate oxidase variant. The method mainly includes steps of contacting the liquid sample with said lactate oxidase variant; measuring a current generated by the reaction between the lactate oxidase variant and the lactate in the liquid sample; and determining the concentration of lactate in the liquid sample by interpolating or extrapolating the current with that of a control sample having a known concentration of lactate.

The application relates to a preparation method and application of a copper ion dynamic coordination lactic acid oxidase loaded injectable hydrogel, and belongs to the technical field of biomedical materials and tumor treatment. A natural biological macromolecule containing a phenolic hydroxyl compound, a solution formed by a low eutectic solvent formed by a hydrogen bond donor and a hydrogen bond acceptor and water, a copper death induction core (Cu 2+ ) and a metabolic regulation and synergistic unit LOx. Cu 2+ Through the cooperation of metal coordination and hydrogen bond, a crosslinked network is formed with the prepared solution, and then LOx is loaded in the hydrogel. For the first time, lactic acid oxidase LOx and copper ions Cu 2+ are integrated in the same injectable hydrogel system to construct a cascade amplification type metabolic reprogramming path. The design not only overcomes the shortcomings of a single copper death inducer, but also fundamentally destroys the metabolic barrier and antioxidant defense of a tumor through LOx driven lactic acid metabolic remodeling, so that the synergy of metabolic intervention and cell death induction is realized.

The application relates to the technical field of biosensors, and discloses a metal nucleic acid framework nanocascade enzyme as well as a preparation method and application thereof. The preparation method of the nanocascade enzyme is as follows: nucleotide sequences shown in SEQ ID NO. 1-10 are mixed in an equimolar ratio, annealing reaction is carried out to obtain a metal template DNA nanosheet; the DNA nanosheet is reacted with a metal precursor to obtain the metal nucleic acid framework nanocascade enzyme. The metal nucleic acid framework nanocascade enzyme DNS-Fe has catalase activity, after corresponding aptamers are added, the DNS-Fe can accurately recognize target objects in various interferents, and the accuracy is high; DNS-Pt / Fe has lactic acid oxidase, glucose oxidase and sarcosine oxidase activity; DNS-Ag / Fe has uric acid oxidase activity; the combination of the DNS-Fe and the DNS-Ag / Fe can realize cascade action, replace natural catalase to catalyze hydrogen peroxide to generate free radicals, and the free radicals are reacted with chromogenic substances to generate a color reaction, and the results are easy to observe.

The invention belongs to the field of biological ceramic materials, and relates to a biological ceramic composite scaffold with a function of catalytically regulating lactic acid metabolism as well as a preparation method and application thereof. The biological ceramic composite scaffold with the function of catalytically regulating lactic acid metabolism comprises a ceramic scaffold matrix with gaps and enzyme-loaded hydrogel filled in the gaps of the ceramic scaffold matrix, and the enzyme-loaded hydrogel is hydrogel formed by loading lactate oxidase and catalase in macromolecular hydrogel. The composite scaffold can catalyze lactic acid to generate pyruvic acid, so that a lactic acid excess bone microenvironment at the initial stage of injury is adjusted, and further physiological behaviors of various cells such as macrophages and mesenchymal stem cells are adjusted, so that a bone microenvironment beneficial to bone defect repair is created, and osteogenesis is promoted.

This invention discloses a radiosensitizer with multi-enzyme activity, its preparation method, and its application in the preparation of antitumor agents. The radiosensitizer comprises rhodium-copper alloy nanoparticles, trithiol-terminated polymethacrylic acid modified on the surface of the rhodium-copper alloy nanoparticles, and lactate oxidase bound to the trithiol-terminated polymethacrylic acid. The preparation method involves mixing rhodium-copper alloy nanoparticles with trithiol-terminated polymethacrylic acid bound to their surface, 1-ethyl-(3-dimethylaminopropyl)carbodiimide, and N-hydroxysuccinimide ester, stirring, adding lactate oxidase, and continuing stirring to obtain the radiosensitizer. This invention achieves precise assembly of the nanocarrier and enzyme-active components through modular design. This invention provides an efficient and safe solution for integrated tumor diagnosis and treatment, and has significant potential for industrial production.

The invention discloses a cascaded nano-enzyme with metabolic reprogramming as well as a preparation method and application of the cascaded nano-enzyme. The method comprises the following steps: firstly, synthesizing manganese and cobalt-based Prussian blue nanoparticles by adopting a precipitation method, and then carrying out calcination treatment in an ammonia gas atmosphere to finally obtain the target nano-enzyme. The nano-enzyme has the catalytic activity of lactate oxidase (LOX) and catalase (CAT) at the same time, and continuous cascade reactions of oxygen, lactic acid, pyruvic acid and hydrogen peroxide and hydrogen peroxide, oxygen and water can be achieved. Compared with a natural LOX enzyme with the same mass concentration, the nano enzyme shows more excellent catalytic activity and temperature stability in an acid environment (pH = 5). The nano enzyme has the following advantages: 1) metabolism regulation is realized through cascade catalytic reaction, and lactic acid is effectively consumed; 2) an anti-oxidation treatment effect is achieved by removing hydrogen peroxide; and 3) the pathological microenvironment of inflammatory tissues can be obviously improved. The invention provides a novel nano-enzyme solution for metabolism regulation and anti-oxidation treatment.

This invention discloses albumin nanoparticles co-loaded with flavin and lactate oxidase, their preparation method, and applications. Using human serum albumin as a carrier, flavin and lactate oxidase are simultaneously encapsulated at a mass ratio of 10:1, with a particle size of 150-180 nm and a polydispersity index of less than 0.2. This invention exerts its anti-hepatocellular carcinoma effect through mechanisms such as reducing lactate levels in the tumor microenvironment, inducing an increase in reactive oxygen species, inhibiting cGAS protein lactation modification, and activating the cGAS-STING signaling pathway. The nanoparticles exhibit high encapsulation efficiency and good stability. The flavin loaded on these nanoparticles can significantly reduce the reduction of pyruvate to lactate by inhibiting lactate dehydrogenase; simultaneously, the lactate oxidase loaded on these nanoparticles catalyzes the dehydrogenation of lactate to pyruvate. In other words, these nanoparticles synergistically regulate lactate metabolism through a two-way regulatory strategy of "reducing source and increasing sink"—"inhibiting lactate production" and "promoting lactate clearance"—significantly enhancing in vitro and in vivo anti-tumor effects, and demonstrating high biosafety.

A composite material contains: bovine serum albumin modified with neutral red; single-walled carbon nanotube inclusions; and lactate oxidase enzyme inclusions. A device for quantitatively assessing lactate content in body fluids comprises a working electrode, the surface of which is coated with the composite material according to the claimed invention. The technical result is that of increasing the upper limit of the range of detectable concentrations of lactate in body fluids to 57 mM, as well as reducing the time taken to perform a single measurement using the claimed device.

The application discloses a method for separating and extracting sodium pyruvate from enzyme conversion liquid for producing sodium pyruvate, which uses enzyme conversion liquid containing sodium pyruvate produced by catalyzing lactic acid with lactic acid oxidase as raw material, removes bacteria bodies, concentrates, sprays, dries and extracts the enzyme conversion liquid with an organic solvent, and comprises the following steps: 1) removing bacteria bodies by filtering the enzyme conversion liquid containing sodium pyruvate through a ceramic membrane; 2) adding activated carbon to perform decolorization treatment; 3) performing concentration by vacuum distillation, so that the content of sodium pyruvate in the enzyme conversion liquid is concentrated to 500-700 g / L; 4) performing spray drying; 5) adding an organic solvent, performing heating, stirring and reflux extraction, cooling to room temperature, centrifugation and drying, and thus pure sodium pyruvate is obtained. The method is simple in operation, low in organic solvent consumption and capable of completely recovering the organic solvent, the produced sodium pyruvate is white in color, the content of the sodium pyruvate is more than 99.6%, the one-time yield is about 96%, the method is low in energy consumption, high in yield and high in product purity, and can be large-scale industrialized production.