5 results about "Lactate oxidase" patented technology

Disclosed herein is directed to a lactate oxidase variant that exhibits reduced affinity to lactate as compared to a wild-type lactate oxidase. The lactate oxidase variant has one or more amino acid substitutions occurring at positions 95, 96, and / or 175 of the wild-type amino acid sequence. Also disclosed herein is a method for detecting and quantifying lactate in various liquid samples by using the present lactate oxidase variant. The method mainly includes steps of contacting the liquid sample with said lactate oxidase variant; measuring a current generated by the reaction between the lactate oxidase variant and the lactate in the liquid sample; and determining the concentration of lactate in the liquid sample by interpolating or extrapolating the current with that of a control sample having a known concentration of lactate.

This invention provides a method for preparing and applying a nanosystem for regulating the osteogenic microenvironment. The nanosystem comprises catalase, lactate oxidase, and a strontium-containing mineralized nanolayer. Experiments demonstrate that this nanosystem can effectively treat bone aging-related diseases, and that catalase and lactate oxidase have a synergistic effect. This invention provides a novel treatment strategy for bone aging-related diseases and has broad prospects for clinical translation.

This invention discloses albumin nanoparticles co-loaded with flavin and lactate oxidase, their preparation method, and applications. Using human serum albumin as a carrier, flavin and lactate oxidase are simultaneously encapsulated at a mass ratio of 10:1, with a particle size of 150-180 nm and a polydispersity index of less than 0.2. This invention exerts its anti-hepatocellular carcinoma effect through mechanisms such as reducing lactate levels in the tumor microenvironment, inducing an increase in reactive oxygen species, inhibiting cGAS protein lactation modification, and activating the cGAS-STING signaling pathway. The nanoparticles exhibit high encapsulation efficiency and good stability. The flavin loaded on these nanoparticles can significantly reduce the reduction of pyruvate to lactate by inhibiting lactate dehydrogenase; simultaneously, the lactate oxidase loaded on these nanoparticles catalyzes the dehydrogenation of lactate to pyruvate. In other words, these nanoparticles synergistically regulate lactate metabolism through a two-way regulatory strategy of "reducing source and increasing sink"—"inhibiting lactate production" and "promoting lactate clearance"—significantly enhancing in vitro and in vivo anti-tumor effects, and demonstrating high biosafety.

Disclosed herein is directed to a lactate oxidase variant that exhibits reduced affinity to lactate as compared to a wild-type lactate oxidase. The lactate oxidase variant has one or more amino acid substitutions occurring at positions 95, 96, and / or 175 of the wild-type amino acid sequence. Also disclosed herein is a method for detecting and quantifying lactate in various liquid samples by using the present lactate oxidase variant. The method mainly includes steps of contacting the liquid sample with said lactate oxidase variant; measuring a current generated by the reaction between the lactate oxidase variant and the lactate in the liquid sample; and determining the concentration of lactate in the liquid sample by interpolating or extrapolating the current with that of a control sample having a known concentration of lactate.