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Preparation method of lactic acid biosensor

A biosensor and lactic acid technology, applied in the field of preparation of lactic acid biosensors, can solve the problems of weak binding force, low stability, difficult electron transmission, etc., and achieve the effects of wide linear range, high repeatability and simple production.

Active Publication Date: 2021-01-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prussian blue and its analogues have been widely used in electrochemical sensing. However, the poor conductivity of Prussian blue greatly limits the transfer of electrons in the field of electrochemical sensing.
Although there have been many cases of combining PB with other nanomaterials to increase its conductivity, most of them are chemically synthesized. The bonding force between the two materials is not strong, the stability is not high, and electron transport is difficult.

Method used

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  • Preparation method of lactic acid biosensor
  • Preparation method of lactic acid biosensor
  • Preparation method of lactic acid biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Polish the gold electrode with polishing powder on metallographic polishing flannelette until the surface is smooth without scratches, ultrasonically treat in deionized water and ethanol respectively, and finally rinse with deionized water. Take 6 μL of copper nanowire solution drop-coated on the polished gold electrode, rinse the electrode surface with deionized water after drying at room temperature. The preparation contains 50mM KCl, 10mM K 3 Fe(CN) 6 , 0.1M hydrochloric acid electrodeposition solution, three electrodes composed of modified electrode, silver / silver chloride reference electrode and platinum counter electrode are immersed in the electrodeposition solution for electrodeposition, and chronoamperometry electrodeposition is performed on the electrochemical workstation. A potential of 0.1V was selected for electrodeposition for 1000 seconds, and the modified electrode after electrodeposition was taken out and rinsed with deionized water. Formulated with 5...

Embodiment 2

[0027] This example is the same as the treatment of copper nanowires and gold electrodes in Example 1, except that 8 μL of copper nanowire solution is drip-coated on the polished gold electrode, and after drying at room temperature, rinse the electrode with deionized water surface. Prepared with 50mM KCl , 50mM K 4 Fe(CN) 6 , 0.1M sulfuric acid electrodeposition solution, chronoamperometry electrodeposition was performed on the electrochemical workstation, and a potential of 0.2V was selected for electrodeposition for 800 seconds, and the modified electrode after electrodeposition was taken out and rinsed with deionized water. Formulated with 8% Nafion ® and 4% BSA lactate oxidase solution, the final enzyme concentration is 150U / mL, take 5 μL enzyme solution and drop it on the surface of the modified electrode, so that the enzyme solution spreads evenly on the electrode surface, and place it in the refrigerator to dry at 4 °C to obtain the lactic acid biosensor.

[0028] Co...

Embodiment 3

[0030] This example is the same as the treatment of copper nanowires and gold electrodes in Example 1, except that 10 μL of copper nanowire solution is drip-coated on the polished gold electrode, and after drying at room temperature, rinse the electrode with deionized water surface. Prepared with 50mM KCl , 100mM K 3 Fe(CN) 6 , 0.1M nitric acid electrodeposition solution, chronoamperometry electrodeposition was performed on the electrochemical workstation, and a potential of 0.3V was selected for electrodeposition for 500 seconds, and the modified electrode after electrodeposition was taken out and rinsed with deionized water. Formulated with 10% Nafion ® and 6% BSA lactic acid oxidase solution, the final enzyme concentration is 250U / mL, take 5 μL enzyme solution and drop it on the surface of the modified electrode, so that the enzyme solution spreads evenly on the electrode surface, and place it in the refrigerator to dry at 4 °C to obtain the lactic acid biosensor.

[003...

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Abstract

The invention relates to a preparation method of a lactic acid biosensor, and belongs to the technical field of lactic acid or lactate detection. The method comprises the following specific steps: preparing a copper nanowire modified electrode, electrodepositing the copper nanowire modified electrode to grow copper-iron prussian blue in situ, and immobilizing lactate oxidase. On the basis of an electrochemical deposition method, Cu / FePBA grows in situ on a certain amount of copper nanowires through electro-deposition, and growth of the nano material is regulated and controlled by adjusting theelectro-deposition method and electro-deposition conditions, so that the catalytic activity and conductivity of the material are regulated and controlled. According to the invention, the biosensing electrode with high catalytic activity and high conductivity is prepared by utilizing an electro-deposition technology, and the high-performance lactic acid biosensor is prepared by combining lactic acid oxidase. The method disclosed by the invention is simple in preparation and high in repeatability, and the prepared lactic acid biosensor has relatively high sensitivity and relatively wide linearrange on lactic acid and lactate.

Description

technical field [0001] The invention relates to a preparation method of a lactic acid biosensor, belonging to the technical field of lactic acid or lactate detection. Background technique [0002] Lactic acid and its lactate widely exist in substrates and products in the fermentation industry, and its concentration affects the quality of fermentation products. Therefore, real-time monitoring of lactic acid concentration is required in the fermentation industry. Lactic acid is a very important physiological indicator for the human body. The level of blood lactic acid can indicate whether the human body is in a healthy state. For example, the blood lactic acid content of people infected with new coronary pneumonia will increase significantly. Regarding lactic acid detection technology, many companies still use traditional chromatography and spectroscopy methods, which are time-consuming, expensive equipment, require professional training for employees, and detection has a cert...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 储震宇冯惊雨金万勤张思健
Owner NANJING UNIV OF TECH
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