High-sensitivity lactic acid determination method and lactic acid detection kit

A lactic acid detection and kit technology, applied in the field of lactic acid bacteria fermented food determination and medical inspection, can solve the problems of poor accuracy, complicated chemical method operation, long time and so on.

Pending Publication Date: 2019-10-18
浙江曼薇尼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method is complicated to operate, takes a long time, and has poor accuracy; the gas chromatography method has good accuracy and less sample consumption, but requires a gas chromatograph and cannot be used in a conventional laboratory; the electrochemical method has high sensitivity, wide linea

Method used

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  • High-sensitivity lactic acid determination method and lactic acid detection kit
  • High-sensitivity lactic acid determination method and lactic acid detection kit
  • High-sensitivity lactic acid determination method and lactic acid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Reagent one:

[0041]

[0042] Reagent 2:

[0043]

[0044] TOOS: N-ethyl-N-(2-hydroxy-3-propanesulfo)-m-toluidine

[0045] Reagent 1: Reagent 2 is 2:1, two-point endpoint method, positive reaction, wavelength: 546nm, tested sample: Reagent 1: Reagent 2 is 3.0: 180: 90, temperature 37°C, serum reacts with Reagent 1 for 5 minutes, Add reagent two, react for 5 minutes, and measure the absorbance.

Embodiment 2

[0047] Reagent one:

[0048]

[0049] Reagent 2:

[0050]

[0051] Reagent 1: Reagent 2 is 3:1, two-point endpoint method, positive reaction, the wavelength is 520nm, the sample to be tested: Reagent 1: Reagent 2 is 4.0: 270: 90, temperature 37°C, serum and reagent 1 react for 5 minutes, Add reagent two, react for 5 minutes, and measure the absorbance.

Embodiment 3

[0053] Reagent one:

[0054]

[0055]

[0056] Reagent 2:

[0057]

[0058] ADPS: Sodium N-ethyl-N-(3-sulfopropyl)-3-methoxyanilide

[0059] Reagent 1: Reagent 2 is 4:1, two-point endpoint method, positive reaction, the wavelength is 550nm, the sample to be tested: Reagent 1: Reagent 2 is 2.5: 200: 50, the temperature is 37°C, serum and reagent 1 react for 5 minutes, Add reagent two, react for 5 minutes, and measure the absorbance.

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Abstract

The present invention relates to a method for measuring lactic acid with high sensitivity, also relates to a lactic acid detection kit, and belongs to the technical fields of medical inspection and lactic acid bacterium fermented food measurement. The lactic acid detection kit is an important detection index for high altitude reaction, athlete training, severe diabetes and body acidosis caused byother reasons, and also an index for detecting lactic acid fermented food. The method uses an original lactate oxidase method, then pyruvate oxidase is introduced, one molecule of lactic acid reacts with the lactate oxidase and pyruvate oxidase to generate two molecules of hydrogen peroxide, then a Trinder reaction is conducted to generate red color, absorbance at a wavelength of 500-600 nm is detected, and content of the lactic acid is calculated. The method has advantages of high sensitivity, good repeatability, wide linearity and good stability, and is very practical for routine laboratoryapplication.

Description

technical field [0001] The invention relates to a method for measuring lactic acid, and at the same time, the invention also relates to a lactic acid detection kit, which belongs to the technical field of medical inspection and the determination of lactic acid bacteria fermented food. Background technique [0002] Lactic acid is the anaerobic glycolysis of glucose to produce pyruvate, which is then reduced. It is an intermediate product of glucose metabolism, mainly derived from skeletal muscle, brain, skin, renal medulla and red blood cells. The concentration of lactic acid in the blood is related to the rate of lactic acid produced by these tissues and the rate of metabolism of lactic acid by the liver. About 65% of lactic acid is metabolized by the liver. Determination of lactic acid concentration in plasma has important diagnostic significance for lactic acidosis. [0003] When the plateau is hypoxic, the body’s blood sugar undergoes anaerobic glycolysis to produce a la...

Claims

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Application Information

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IPC IPC(8): C12Q1/28C12Q1/26
CPCC12Q1/28C12Q1/26C12Q2326/96
Inventor 李丰盛倪建芬黄丽珍
Owner 浙江曼薇尼生物科技有限公司
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