Electrochemical biosensor and preparation method thereof

A biosensor and electrochemical technology, applied in the field of analysis, can solve problems such as poor curing effect and complicated fixing methods, and achieve the effects of excellent enzyme immobilization time, good cross-linking effect, and high enzyme activity

Active Publication Date: 2021-07-20
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the current fixing method is very complicated, and the curing effect is not good

Method used

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  • Electrochemical biosensor and preparation method thereof
  • Electrochemical biosensor and preparation method thereof
  • Electrochemical biosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0075] 1. Preparation of Enzyme Layer

[0076] Preparation of enzyme solution containing glucose oxidase / lactate oxidase: dissolve glucose oxidase / lactate oxidase in water at a concentration of 0.5-2wt%, and the enzyme solution contains 1wt% calcium chloride.

[0077] Print, dispense glue, or apply enzyme on the working electrode, and coat it with an enzyme solution containing glucose oxidase / lactate oxidase; make a layer of enzyme film on the working electrode, and then bake it in a 65-degree oven for 10 minutes.

[0078] 2. Preparation of carrier layer:

[0079] Carboxymethyl chitosan was placed in water and soaked for 24 hours, stirred in a mechanical stirrer for 30-60min to obtain carboxymethyl chitosan aqueous solution;

[0080] Silica is dispersed in water for 30 minutes, homogenized by a high-speed homogenizer at 3000 r / min; the concentration of silica is 0.1 wt%.

[0081] After soaking the agarose gel in water for 1-3 days, stir it with a mechanical stirrer for 30-60...

Embodiment 1

[0093] The material composition and concentration of enzyme layer, carrier layer and cross-linked layer are respectively:

[0094] 1 enzyme layer: 1wt% glucose oxidase;

[0095] 2. Carrier layer: 1wt% carboxymethyl chitosan;

[0096] 3. Cross-linked layer: 1 wt% glutaraldehyde.

[0097] Test results: The number of days for immobilizing enzymes that meets the requirements is 13 days. It shows that adding a certain concentration of high polymer has a certain effect on the effect of immobilizing enzymes.

Embodiment 2

[0099] The material composition and concentration of enzyme layer, carrier layer and cross-linked layer are respectively:

[0100] 1 enzyme layer: 1wt% glucose oxidase;

[0101] 2. Carrier layer: silicon dioxide with a concentration of 0.1wt%;

[0102] 3. Cross-linked layer: 1 wt% glutaraldehyde.

[0103] Test results: The number of days for immobilizing enzymes that meets the requirements is 12 days. It shows that adding a certain concentration of high polymer has a certain effect on the effect of immobilizing enzymes.

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Abstract

The invention relates to an electrochemical biosensor and a preparation method thereof. The electrochemical biosensor comprises a working electrode, and an enzyme layer, a carrier layer and a cross-linking layer are sequentially formed on the working electrode; the enzyme layer comprises glucose oxidase or lactate oxidase; the carrier layer is prepared from raw materials including at least one of chitosan, silicon dioxide, agarose and alginic acid or salts thereof; an the cross-linking layer is prepared from a raw material comprising a cross-linking agent. The electrochemical biosensor disclosed by the invention does not fall off or degrade after being monitored in vivo for a long time, and can keep the current stable for a long time.

Description

technical field [0001] The invention relates to the field of analysis, in particular to an electrochemical biosensor and a preparation method thereof. Background technique [0002] Biosensor is an analysis system that uses electrochemical principles to use enzymes, antibodies, antigens, microorganisms, nucleic acids and other biologically active substances as sensitive elements, and converts their concentrations into electrical signals to quickly, accurately and conveniently measure the content of biological substances. The constituent materials Must have specific properties in order to function in vivo and provide the appropriate signal. Electrochemical biosensors have been widely used in clinical diagnosis, food and drug analysis, and environmental control. [0003] In recent years, glucose sensors, especially implantable glucose sensors, have received extensive attention. Such sensors are of great significance in clinical applications, especially in monitoring diabetes....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/31
CPCG01N27/3272G01N27/31
Inventor 吴岸峰景振辉赖远强
Owner GUANGZHOU WONDFO BIOTECH
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