9 results about "Nano biosensor" patented technology

The invention relates to the technical field of nano biosensors, and discloses a bimodal nano probe for uric acid detection as well as a preparation method and application of the bimodal nano probe. The bimodal nano-probe is a fluorescent-photo-thermal bimodal nano-probe, is a core-shell structure nano-particle, and sequentially comprises the following components from the inner layer to the outer layer: a) a fluorescent core which is composed of a carbon dot and a silicon dioxide layer coating the carbon dot; b) a manganese dioxide layer coated outside the fluorescent inner core; and c) a molecularly imprinted polymer layer coated outside the manganese dioxide layer. According to the bimodal nanoprobe disclosed by the invention, through ingenious structural design, a fluorescence signal source (carbon dots), a signal modulation and photo-thermal unit and a specific recognition layer (uric acid molecularly imprinted polymer) are integrated, so that bimodal sensing of'specific recognition-> MnO2 etching-> fluorescence recovery / photo-thermal weakening 'on UA is realized; the problems of poor selectivity, complicated operation and high cost of a traditional method are effectively solved.

The invention discloses an in-vitro prediction method for cell DNA damage caused by simulated radiation. According to the invention, by introducing the bionic oxidative stress regulation and control module, the simulation precision of the in-vitro model on the in-vivo physiological microenvironment is improved. The dynamic microenvironment intervention system can respond to oxidative stress changes in the radiation process in real time, the redox state of cells is closer to an in-vivo real radiation exposure scene by precisely regulating and controlling the perfusion rate of the active oxygen scavenger, experimental deviation caused by out-of-control oxidative stress level in traditional static culture is avoided, and the experimental accuracy is improved. A more reliable physiological basis is provided for subsequent damage signal detection, a dynamic regulation and control mechanism of the micro-fluidic chip can effectively correct baseline drift caused by oxidative stress fluctuation, and it is ensured that damage site space distribution data captured by the nano biosensor array has a higher signal-to-noise ratio; the influence of external environment interference on experimental data is reduced, and high-quality original data support is provided for follow-up repair trajectory tracking and neural network prediction.

The invention is notably directed to a computer-implemented method of optimizing an optical sensor (1) comprising a single-walled carbon nanotube (2), or SWCNT for short, which is wrapped in single-stranded sequence (3) of nucleotides (ssSN). The method revolves around two or more optimization cycles. Each optimization cycle includes clustering ssSNs of an input set of ssSNs to obtain clusters and selecting representative ssSNs of the clusters obtained. Next, one accesses measurements of optical responses of optical sensors to a target molecule, where the optical sensors include SWCNTs wrapped in actual ssSNs, which are synthetized in accordance with the representative ssSNs selected. After that, a top ssSN is identified among the representative ssSNs. The top ssSN is one that led to the highest optical response. Importantly, the top ssSN identified at each optimization cycle (but the last cycle) is mutated to obtain mutants and form a superset of sequences including the top ssSN and its mutants, prior to performing the next optimization cycle, using this superset as a new input set of ssSNs. This way, a local optimum is identified in the initial input space of the ssSNs. Additional optimization can be achieved through supervised learning and pattern recognition. The invention is further directed to related engineering methods (to synthetize ssSNs and wrap SWCNTs) and computer programs (to perform the optimizations).

The invention belongs to the technical field of microbiological detection, and discloses a nanometer biosensor based on PfAgo and SERS aggregation-induced enhancement (AiEn) signal amplification, a salmonella typhimurium detection method and application, and the nanometer biosensor comprises an LAMP amplification system and a PfAgo reaction system. The detection method established by the invention is high in specificity, and by taking salmonella typhimurium detection as an example, no obvious fluorescence signal exists on microorganisms such as escherichia coli, saccharomycetes and agrobacterium tumefaciens, so that the salmonella typhimurium detection primer has good selectivity on salmonella typhimurium and is not influenced by other microorganisms.

The invention discloses a nano biosensor based on functionalized graphdiyne, construction of the nano biosensor and application of the nano biosensor in bacterial extracorporeal vesicle detection, and belongs to the technical field of nano biosensors. The invention relates to a nano biosensor based on functionalized graphdiyne (GDY). The nano biosensor comprises GDY-metal oxide nano enzyme, graphdiyne is used as a substrate material, and metal oxide nanoparticles are loaded on the surface of the substrate material to form the heterogeneous composite material with a stable structure. According to the sensor, through rational design of a GDY-based nano sensing interface, efficient capture of bacterial extracellular vesicles is realized under the condition of not depending on ultracentrifugation, and eukaryotic extracellular vesicles and bacterial-derived extracellular vesicles are effectively distinguished. In order to reduce the detection cost, various metal nanoparticles are adopted for functional modification, and the capture process can be completed without using expensive biological recognition molecules.

The application belongs to the field of nano-biosensors, and discloses an aptamer sensor for chronoamperometry detection, a preparation method and application thereof. Gold nanoparticles are loaded on conductive glass by using in-situ reduction method, and then DNA aptamer is modified. The DNA aptamer can specifically adsorb target bacteria in a test solution on the surface of the material and generate a current response linearly related to the concentration of the bacteria solution. A PBS buffer solution containing MgCl2 is used as an electrolyte, and the chronoamperometry method can be used to measure the current response of different concentrations of Staphylococcus aureus. The new nano-biosensor provides a practical new method for on-site rapid detection of bacteria, and can be widely used in the fields of food detection and environmental detection.

Disclosed is a device including a transistor that includes a graphene-based conducting channel, a functional layer modified with an aptamer, and attached to the graphene-based conducting channel via a polymer, such that the aptamer binds with a target biomarker. A method for manufacturing an aptamer-based biosensor, and a method for detecting a target biomarker using the aptamer-based biosensor, are also provided.

The online biological aerosol monitoring device comprises an instrument body and an air inlet pipe, the air inlet pipe is arranged on the instrument body, a cover ring is arranged at the other end of the air inlet pipe, an impurity filter screen is arranged between the cover ring and the air inlet pipe, and the air inlet pipe is arranged on the instrument body. A connecting assembly used for moving and positioning the cover ring is arranged on the side face of the air inlet pipe, and the cover ring is arranged on the connecting assembly. According to the biological aerosol detection device, the cover ring and the impurity filter screen are arranged, and the impurity filter screen is mounted at the air inlet of the air inlet pipe through the cover ring, so that impurities in the air can be pre-filtered by the impurity filter screen when air enters the air inlet pipe, and the impurities influencing biological aerosol detection are left outside; the related analysis operation of the instrument body on the biological aerosol is not easily influenced, and the problem that the accuracy of the analysis result is influenced by dust and impurities in the air due to the fact that the existing online biological aerosol monitoring device of the nano-biosensor directly sucks the air is solved.

The invention relates to the technical field of biomedicine, and discloses an HPV (human papillomavirus) infected inflammation removal and detoxification method which comprises the following steps: constructing a nano biosensor which comprises a gold nanoparticle substrate material, and the surface of the nano biosensor is modified with a biological probe capable of specifically recognizing HPV specific protein and inflammation markers; the nano biosensor is introduced into an infected part in a patient body, so that the nano biosensor can monitor virus and inflammation indexes of the infected part in real time and transmit monitoring information to external equipment; and screening and applying an immunomodulatory drug, and dynamically adjusting the dosage of the immunomodulatory drug according to the immune cell activity index monitored by the nano biosensor to activate the body immune system. The virus load and the inflammatory marker level of an infected part are monitored in real time through the nano biosensor, and the synergistic effect of virus removal, inflammation repair and immune regulation is achieved in combination with precise delivery of the immune regulation medicine and the nano particle medicine carrier.