13 results about "Nano biosensor" patented technology

The invention discloses a control and alarm method and device for multi-parameter grain condition data and a medium, and relates to the technical field of granary management. The method comprises the following steps: acquiring grain depot data and grain pile data through a sensor, and synchronously acquiring warehouse in-out receipt information; the grain depot data and the grain pile data are compared with a historical database, a dynamic threshold value model is established, and the dynamic threshold value model adjusts a parameter deviation range based on climate data and a storage period of the grain depot in the same region; according to the dynamic threshold model, obtaining a corresponding boundary value, generating a 3D distribution diagram of the grain pile, and calculating an interlayer difference; if the grain depot data and the grain pile data reach boundary values or the interlayer difference exceeds a first preset threshold value, an alarm signal is triggered, and the stock quantity is verified by associating the in-out stock receipt information. According to the method, real-time collection of multi-source data and application of the nano biosensor are achieved, and comprehensive and accurate monitoring of internal and external environmental parameters and grain physiological indexes of the grain depot is achieved through the dynamic threshold value model.

The present invention relates to the technical field of nano-biosensors, and discloses a DNA-mediated synthesized platinum-coated gold nano-bicone colorimetric sensor, a preparation method thereof and an application thereof. The preparation method comprises the following steps: adding a DNA sequence into a gold nano-bicone solution for incubation, then adding H2PtCl6 and ascorbic acid, and incubating again to obtain platinum-coated gold nano-bicones. The obtained platinum-coated gold nano-bicones have peroxidase activity, can be used for preparing peroxidase catalysts, and can be used for preparing colorimetric sensors for detecting alkaline phosphatase. The detection process has mild conditions, does not require other reagents, realizes the convenient and rapid detection of alkaline phosphatase activity, has low detection cost, simple operation, sensitive response to alkaline phosphatase, a detection limit as low as 0.35 mU / mL, and a detectable range as wide as 0-12.5 mU / mL; the measurement results of actual samples are accurate and reliable, and the detection of alkaline phosphatase activity in human serum can be realized.

The invention relates to the technical field of nano biosensors, and discloses a bimodal nano probe for uric acid detection as well as a preparation method and application of the bimodal nano probe. The bimodal nano-probe is a fluorescent-photo-thermal bimodal nano-probe, is a core-shell structure nano-particle, and sequentially comprises the following components from the inner layer to the outer layer: a) a fluorescent core which is composed of a carbon dot and a silicon dioxide layer coating the carbon dot; b) a manganese dioxide layer coated outside the fluorescent inner core; and c) a molecularly imprinted polymer layer coated outside the manganese dioxide layer. According to the bimodal nanoprobe disclosed by the invention, through ingenious structural design, a fluorescence signal source (carbon dots), a signal modulation and photo-thermal unit and a specific recognition layer (uric acid molecularly imprinted polymer) are integrated, so that bimodal sensing of'specific recognition-> MnO2 etching-> fluorescence recovery / photo-thermal weakening 'on UA is realized; the problems of poor selectivity, complicated operation and high cost of a traditional method are effectively solved.

The invention discloses an in-vitro prediction method for cell DNA damage caused by simulated radiation. According to the invention, by introducing the bionic oxidative stress regulation and control module, the simulation precision of the in-vitro model on the in-vivo physiological microenvironment is improved. The dynamic microenvironment intervention system can respond to oxidative stress changes in the radiation process in real time, the redox state of cells is closer to an in-vivo real radiation exposure scene by precisely regulating and controlling the perfusion rate of the active oxygen scavenger, experimental deviation caused by out-of-control oxidative stress level in traditional static culture is avoided, and the experimental accuracy is improved. A more reliable physiological basis is provided for subsequent damage signal detection, a dynamic regulation and control mechanism of the micro-fluidic chip can effectively correct baseline drift caused by oxidative stress fluctuation, and it is ensured that damage site space distribution data captured by the nano biosensor array has a higher signal-to-noise ratio; the influence of external environment interference on experimental data is reduced, and high-quality original data support is provided for follow-up repair trajectory tracking and neural network prediction.

The invention discloses a plant early stress monitoring method based on nano sensing infrared thermal image recognition, which comprises the following steps: capturing a biological signal of a plant by in-situ synthesizing a nano biosensor on the plant, converting absorbed near-infrared laser into heat by the biosensor after the plant is stressed, and making a data set by utilizing a thermal imaging picture of the plant; expanding the data set, dividing the data set into a training set, a test set and a verification set, preprocessing pictures of the training set and the verification set, inputting the pictures into a deep learning model for training, and according to a training result, evaluating the model and optimizing parameters; and finally, inputting the test set into the deep learning model after parameter optimization to obtain a final classification result. According to the method, the nano biosensor, the thermal imaging technology and the deep learning algorithm are fused, the stress of the plant in the early stage can be monitored and warned, the accuracy of monitoring the early stress of the plant is effectively improved, and plant damage and significant output reduction are avoided.

The invention is notably directed to a computer-implemented method of optimizing an optical sensor (1) comprising a single-walled carbon nanotube (2), or SWCNT for short, which is wrapped in single-stranded sequence (3) of nucleotides (ssSN). The method revolves around two or more optimization cycles. Each optimization cycle includes clustering ssSNs of an input set of ssSNs to obtain clusters and selecting representative ssSNs of the clusters obtained. Next, one accesses measurements of optical responses of optical sensors to a target molecule, where the optical sensors include SWCNTs wrapped in actual ssSNs, which are synthetized in accordance with the representative ssSNs selected. After that, a top ssSN is identified among the representative ssSNs. The top ssSN is one that led to the highest optical response. Importantly, the top ssSN identified at each optimization cycle (but the last cycle) is mutated to obtain mutants and form a superset of sequences including the top ssSN and its mutants, prior to performing the next optimization cycle, using this superset as a new input set of ssSNs. This way, a local optimum is identified in the initial input space of the ssSNs. Additional optimization can be achieved through supervised learning and pattern recognition. The invention is further directed to related engineering methods (to synthetize ssSNs and wrap SWCNTs) and computer programs (to perform the optimizations).

The invention belongs to the technical field of microbiological detection, and discloses a nanometer biosensor based on PfAgo and SERS aggregation-induced enhancement (AiEn) signal amplification, a salmonella typhimurium detection method and application, and the nanometer biosensor comprises an LAMP amplification system and a PfAgo reaction system. The detection method established by the invention is high in specificity, and by taking salmonella typhimurium detection as an example, no obvious fluorescence signal exists on microorganisms such as escherichia coli, saccharomycetes and agrobacterium tumefaciens, so that the salmonella typhimurium detection primer has good selectivity on salmonella typhimurium and is not influenced by other microorganisms.

The invention discloses an SERS (Surface Enhanced Raman Scattering) magnetic nano biosensor based on assistance of a catalytic hairpin assembly technology and an application of the SERS magnetic nano biosensor in circRNA (Ribonucleic Acid) detection. According to the technical thought, magnetic nanoparticles Fe3O4 (at) Ag (at) DTBN MNPs with a self-calibration function are prepared, silver-coated gold nanoparticles with Raman signal molecules are prepared through a seed method, then corresponding hairpin DNA structures are designed according to an anti-shearing site sequence of circRNA, the hairpin DNA structures are modified on the surfaces of the silver-coated gold nanoparticles and the surfaces of Fe3O4 (at) Ag (at) DTBN MNPs respectively, and the magnetic nanoparticles Fe3O4 (at) Ag (at) DTBN MNPs with the self-calibration function are obtained. According to the method, hairpin DNA (deoxyribonucleic acid) HP1 chain functionalized coded magnetic nanoparticles Fe3O4 (at) Ag (at) DTBN-HP1 MNPs and hairpin DNA HP2 chain functionalized coded silver-coated gold nanoparticles Au (at) Reporter (at) Ag-HP2 NPs are respectively obtained, and a cyclic amplification system is constructed by utilizing enzyme-free isothermal amplification and nuclear satellite structure magnetic SERS (Surface Enhanced Raman Scattering) nanospheres, so that ultrasensitive quantitative detection of circRNA genes is realized. The SERS magnetic nano biosensor provided by the invention has the advantages of high sensitivity, high specificity, high flux, no need of enzyme participation and the like, and can realize rapid detection of one or more circRNAs in complex reaction systems such as serum and the like.

The invention discloses a nano biosensor based on functionalized graphdiyne, construction of the nano biosensor and application of the nano biosensor in bacterial extracorporeal vesicle detection, and belongs to the technical field of nano biosensors. The invention relates to a nano biosensor based on functionalized graphdiyne (GDY). The nano biosensor comprises GDY-metal oxide nano enzyme, graphdiyne is used as a substrate material, and metal oxide nanoparticles are loaded on the surface of the substrate material to form the heterogeneous composite material with a stable structure. According to the sensor, through rational design of a GDY-based nano sensing interface, efficient capture of bacterial extracellular vesicles is realized under the condition of not depending on ultracentrifugation, and eukaryotic extracellular vesicles and bacterial-derived extracellular vesicles are effectively distinguished. In order to reduce the detection cost, various metal nanoparticles are adopted for functional modification, and the capture process can be completed without using expensive biological recognition molecules.

The application belongs to the field of nano-biosensors, and discloses an aptamer sensor for chronoamperometry detection, a preparation method and application thereof. Gold nanoparticles are loaded on conductive glass by using in-situ reduction method, and then DNA aptamer is modified. The DNA aptamer can specifically adsorb target bacteria in a test solution on the surface of the material and generate a current response linearly related to the concentration of the bacteria solution. A PBS buffer solution containing MgCl2 is used as an electrolyte, and the chronoamperometry method can be used to measure the current response of different concentrations of Staphylococcus aureus. The new nano-biosensor provides a practical new method for on-site rapid detection of bacteria, and can be widely used in the fields of food detection and environmental detection.

Disclosed is a device including a transistor that includes a graphene-based conducting channel, a functional layer modified with an aptamer, and attached to the graphene-based conducting channel via a polymer, such that the aptamer binds with a target biomarker. A method for manufacturing an aptamer-based biosensor, and a method for detecting a target biomarker using the aptamer-based biosensor, are also provided.

The online biological aerosol monitoring device comprises an instrument body and an air inlet pipe, the air inlet pipe is arranged on the instrument body, a cover ring is arranged at the other end of the air inlet pipe, an impurity filter screen is arranged between the cover ring and the air inlet pipe, and the air inlet pipe is arranged on the instrument body. A connecting assembly used for moving and positioning the cover ring is arranged on the side face of the air inlet pipe, and the cover ring is arranged on the connecting assembly. According to the biological aerosol detection device, the cover ring and the impurity filter screen are arranged, and the impurity filter screen is mounted at the air inlet of the air inlet pipe through the cover ring, so that impurities in the air can be pre-filtered by the impurity filter screen when air enters the air inlet pipe, and the impurities influencing biological aerosol detection are left outside; the related analysis operation of the instrument body on the biological aerosol is not easily influenced, and the problem that the accuracy of the analysis result is influenced by dust and impurities in the air due to the fact that the existing online biological aerosol monitoring device of the nano-biosensor directly sucks the air is solved.

The invention relates to the technical field of biomedicine, and discloses an HPV (human papillomavirus) infected inflammation removal and detoxification method which comprises the following steps: constructing a nano biosensor which comprises a gold nanoparticle substrate material, and the surface of the nano biosensor is modified with a biological probe capable of specifically recognizing HPV specific protein and inflammation markers; the nano biosensor is introduced into an infected part in a patient body, so that the nano biosensor can monitor virus and inflammation indexes of the infected part in real time and transmit monitoring information to external equipment; and screening and applying an immunomodulatory drug, and dynamically adjusting the dosage of the immunomodulatory drug according to the immune cell activity index monitored by the nano biosensor to activate the body immune system. The virus load and the inflammatory marker level of an infected part are monitored in real time through the nano biosensor, and the synergistic effect of virus removal, inflammation repair and immune regulation is achieved in combination with precise delivery of the immune regulation medicine and the nano particle medicine carrier.