81 results about "Nano biosensor" patented technology

The present invention is directed to systems and methods for detecting biological and chemical species in liquid and gaseous phase. The systems and methods utilize carbon nanotubes to enhance sensitivity and selectivity towards the reacting species by decreasing interference and detecting a wide range of concentrations.

The present invention is directed to systems and methods for detecting biological and chemical species in liquid and gaseous phase. The systems and methods utilize carbon nanotubes to enhance sensitivity and selectivity towards the reacting species by decreasing interference and detecting a wide range of concentrations.

An apparatus for detecting multiple analytes comprising an array of nanobiosensors, each comprising a biological entity immobilized onto carbon nanotubes, wherein a plurality of the nanobiosensors in the array have unique biological entities, wherein a first one of the plurality of nanobiosensors has a first biological entity immobilized onto carbon nanotubes, and wherein a second one of the plurality of nanobiosensors has a second biological entity immobilized onto carbon nanotubes, the first biological entity is unique relative to the second biological entity.

The present invention relates to biosensors, in particular to surface-enhanced Raman biosensors for detection of in vivo and ex vivo analytes. In particular, the present invention provides compositions and methods for the in vivo detection of analytes such as glucose.

The present invention relates to a particle comprising a non-metallic core having a fluorescent material and a metallic shell encapsulating the non-metallic core wherein the metallic shell has transparency for an electromagnetic radiation having a first wavelength to excite the said fluorescent material and reflectance for an electromagnetic radiation having a second wavelength emitted by the said fluorescent material to confine the electromagnetic radiation having the second wavelength in the metallic shell. This system allows for the excitation of optical cavity modes inside the particle even at sub-micron particle size. The cavity modes are extremely sensitive to any change of the dielectric environment of the particle. This sensitivity can be used for the construction of optical nano-biosensors. Another application of the system is that of a microscopic source for spherical light waves, which may find applications in digital inline holography and display technology.

The present invention provides a producing method of the nanometer biosensor with high sensitivity. The procedures are as follows: firstly, selecting the fundus material and completing the producing of the metal nanometer array; secondly, soaking the fundus in the prepared biology activated chemical reagent solution to ensure the metal surface possess an activated group corresponding to the biology molecule, and to ensure the combination of the antigen and the silver granules array to be more easy, the biology activated chemical reagent is the octane mercaptan 1-OT and the eleven alcoholic acid 11-MUA with the hydrogen thiocyanato, the solvent is ethanol; thirdly, taking out the soaked substrate, cleaning off the leftover on the surface of the substrate and blow the substrate dry; fourthly, selecting the antigen corresponding to the detected molecule and the zero-distance coupling reagent; fifthly, mixing the antigen solution and the zero-distance coupling reagent solution, and then drip the mixed solution on the surface of the substrate for coupling reaction, and the reaction period is more than 3 hours; sixthly, rinsing the substrate and removing the leftover on the surface of the substrate and blow the substrate dry, and then the producing of the nanometer sensor is completed. The present invention improves greatly the sensitivity of the sensor.

The present invention relates to biosensors, in particular to surface-enhanced Raman biosensors for detection of in vivo and ex vivo analytes. In particular, the present invention provides compositions and methods for the in vivo detection of analytes such as glucose.

The invention discloses a method for detecting Listeria monocytogenes by a multiple cross amplification coupled with gold nanoparticle-based biosensor. According to the method, a 5' end of a cross primer CP1 or CP2 in multiple cross displacement amplification is marked with biotin; a 5' end of an amplification primer C1 or C2 is marked with hapten; and an amplification product for the listeria monocytogenes lmo0733 can be visually detected by a gold nanoparticle-based biosensor. The method is convenient, rapid, sensitive, specific and suitable for detection of various nucleotide fragments.

The invention discloses a fluorescent glucose nano biosensor. Active ingredients of the sensor are functionalized fluorescent nanoparticles. The fluorescent glucose nano biosensor is prepared by combining an enzyme catalysis method and a fluorescent oxygen nano sensor. Fluorescence detection of glucose is indirectly realized based on consumption of glucose oxidase on oxygen in a glucose catalysis process. The glucose nano biosensor has the advantages of high response speed, high sensitivity and high stability, and the lower limit of glucose detection can be 0.5mmol / L. Because the sensor has a nanoscale size and high biocompatibility, the sensor can be used for detecting the concentration of glucose in microenvironments (such as living cells) in organisms and in human body fluid (such as plasma).

The invention relates to a nano biosensor capable of carrying out rapid colorimetric detection on Hg<2+> in water. The nano biosensor is used for carrying out optimized design on a Hg ion specificity oligonucleotide probe on the basis of the Hg ion specificity oligonucleotide probe and nanogold and adsorbing the Hg ion specificity oligonucleotide probe to the surfaces of nanogold particles to realize assembly of the biosensor; the quantitative analysis on Hg<2+> is realized through utilizing stable T-T mispairing of Hg ion specificity and color change of the nanogold particles under the high-salt condition. The nano biosensor is free of marking the probe or modifying the nanogold, can realize visible portable analysis of the Hg ions without any special condition, has the advantages of simpleness and convenience for operation, short detection time, higher sensitivity, strong specificity and the like and is easy to popularize and use.

The invention discloses a multi-cross isothermal amplification and gold nanogold biosensing combined nucleic acid detection technology. A biotin is marked at a 5'-end of an amplification primer C1 in multi-cross replacement isothermal amplification, and a hapten is marked at a 5'-end of D1, so that a double-mark product can be generated and can be visually detected by a nanogold biosensor. The technology is convenient, quick, sensitive and specific and is suitable for detection of various nucleotide fragments and especially can be used for simultaneous detection of various target gene fragments.

The invention provides an electrochemical DNA biosensor for detecting PML / RAR alpha fusion gene of acute promyelocytic leukemia. The gene to be detected is the PML / RAR alpha fusion gene of the acute promyelocytic leukemia (APL); and the method comprises the following steps: (1) designing and synthesizing a specific sequence of the APL according to the selected fusion sites of a universal primer sequence of the gene type of the APL to be detected; and (2) constructing a nano modified electrode by a Au nano material, and constructing the nano electrochemical biosensor used for detecting the PML / RAR alpha fusion gene of the APL by the electrochemical enzyme-linked immunoassay technology. The method greatly improves the sensitivity of the biosensor so as to realize early diagnosis of the acute promyelocytic leukemia.

The invention discloses a sub-terahertz nano biosensor for detecting single or few cells, belonging to the technical field of sensors. The sub-terahertz nano biosensor comprises a terahertz sample detection cell which consists of a microfluid unit and slot nano antennas, wherein the microfluid unit comprises at least one microflow body, a positive pressure generating unit and a negative pressure generating unit; the microflow bodies are of a tubular structure, the positive pressure generating unit is arranged at cell inlet ends of the microflow bodies, and the negative pressure generating unit is arranged at cell outlet ends of the microflow bodies; the slot nano antennas are provided with slots penetrating through antenna bodies; the microflow bodies penetrate through the slots in a one-to-one correspondence manner. By virtue of the terahertz sample detection cell consisting of the microfluid unit and the slot nano antennas, only single or few to-be-detected cells can pass through, the signal of the single cell is amplified and captured, and the cells of different characters are distinguished and identified, so that bacteria of different characters can be really, qualitatively and quantitatively detected by directly utilizing a sub-terahertz sensing technique.

The invention discloses a sub-THz nano-biosensor for quickly frame-detecting bacteria and a detection method thereof, and belongs to the technical field of sensors. The sensor includes a microfluid unit and a sample detection pool consisting of slot nanotube antennas, wherein the microfluid unit includes at least one microfluid body which is with a tubular structure, a positive pressure generating device arranged at the cell inlet end of the microfluid body, and a negative pressure generating device arranged at the cell outlet end of the microfluid body. The invention also discloses a detection method of the sensor, wherein bacterium sample liquid is fully diluted and passes through the microfluid sample detection pool to be subjected to THz spectrum detection. According to the sub-THz nano-biosensor for quickly frame-detecting bacteria and the detection method thereof, enough negative pressure is generated in the microfluid, so that cells to be detected only can pass through the microfluid body with small diameter singly or in small number, so that detection specificity is effectively improved; in addition, the signal of single cell is amplified by the slot nanotube antennas, so that the sensitivity problem of single cell detection is thoroughly solved, and thereby the bacterium cells with different characters can be distinguished and recognized.

The invention provides an electrochemical aptamer sensor for quantitatively detecting ochratoxin A and application thereof, and relates to the field of nano biosensors and electrochemical detection. The electrochemical aptamer sensor is a three-electrode system sensor, the working electrode is obtained by sequentially modifying the surface of a gold sheet electrode with a composite film of reducedgraphene oxide, molybdenum disulfide and nanogold, a capture probe 1, a capture probe 2 and an ochratoxin A aptamer, and every two of the capture probe 1, the capture probe 2 and the ochratoxin A aptamer are complementarily paired to form a Y-shaped structure. The electrochemical aptamer sensor disclosed by the invention is used for quantitatively detecting ochratoxin A and has the advantages ofsimplicity in operation, good stability, high sensitivity, strong specificity, good reproducibility and the like.

The invention provides a nanometer biosensor which is formed by self-assembling of carbon quantum dot-labeled concanavalin and a cholesterol sensor, wherein the cholesterol sensor is prepared by co-incubating cyclodextrin-modified nanogold and rhodamine 6G to remove excessive reactants. Moreover, the invention further provides a preparation method and application of the nanometer biosensor. The nanometer biosensor provided by the invention is established based on a fluorescence resonance energy transfer theory, can synchronously detect glucose and cholesterol, avoids mutual interference in the detection process and is a dual substance quantitative determination sensor which is novel, simple, convenient, low in cost, rapid and high in sensitivity.

This invention relates to a kind of nm bio-sensor and its NADH concentration test method, which takes Au nm particles fixed on the surface of a sensor as the crystal seeds, utilizes the principle of Au / Ag nucleocapsid nm particle catalyst increase under the NADH recovery action and an absorption spectrum variance rule to compute the change of nm particle absorption value to test the NADH concentration.

The invention discloses a multiple cross isothermal amplification and macromolecular nano biosensing combined target gene testing method. According to the method, a half antigen is marked at a 5' terminal of a cross primer CP1 or CP2 in multiple cross displacement amplification, and antarctic thermal sensitive uracil deoxyribonucleic acid glycosylase and biotinylated deoxyuridine are introduced into an amplification system to test amplification products on the basis of multiple cross displacement amplification combined with macromolecular nano biosensing. According to the method, amplification products of HPV16 type E7 gene or HPV18 type L1 gene can be visually tested by a macromolecular nano biosensor. The method is convenient, fast, sensitive, specific and suitable for testing of various nucleotide fragments.

The invention provides a new nano fluorescence biosensor with a biological release control function as well as a preparation method and application thereof, wherein gold nano particles are used as an orifice cap of a gold nano cage. The sensor has the following characteristics: a DNA hybridization technology is adopted, and the gold nano particles are assembled on the surface of the gold nano cage to form an orifice cap switch to realize blocking of matters in the gold nano cage. The nano biosensor provided by the invention has the characteristics of simple structure, sensitive signal, strong controllability and the like. By adopting the nano fluorescence biosensor provided by the invention, high-sensitivity detection of ATP can be realized in a range of 1.0*10<-9>-1.0*10<-7>M; and the nano fluorescence biosensor has the advantages of sensitivity, economy, easiness in operation and the like, gains relatively great application potential and broad application prospects in the fields such as biomedicine and life science, and provides a new way and method for early diagnosis and treatment of serious diseases and particularly for the study and application of tumor-targeted drugs and photo-thermal therapy based on the nano carrier release control technology.

The invention provides amplification primer sets of Klebsiella pneumoniae, application and a method for detecting the Klebsiella pneumoniae. The method for detecting the Klebsiella pneumoniae comprises the following steps: 1) extracting a genome of a Klebsiella pneumoniae sample to be detected; 2) providing the following specific gene rcsA amplification primer sets of the Klebsiella pneumoniae: CP1, CP2, F1, F2, D1, C1, R1, D2, C2, R2, C1*, C2*, D1* and D2*; 3) using genomic nucleic acid of the Klebsiella pneumoniae sample to be detected as a template for isothermal amplification under a multi-crossover isothermal amplification reaction system comprising the amplification primer sets to obtain an amplification product; 4) detecting the amplification product of the step 3) by using a gold nanobiosensor. The method provided by the invention uses multi-crossover isothermal amplification as a basis, is combined with a nanometer biological detection technology, and can rapidly, sensitivelyand highly specifically identify the Klebsiella pneumoniae.

The invention discloses a method for preparing a visual biosensor for detecting organophosphorus pesticides based on an ink-jet printing technology and belongs to the technical field of nano biosensors. The method comprises the steps of preparing a quantum dot solution with negative charge and a polyelectrolyte solution with positive charge; alternately printing the quantum dot solution and the polyelectrolyte solution onto a substrate material to obtain multiple layers of polyelectrolyte / quantum dot thin films; alternately dropping polyelectrolyte and acetylcholin esterase onto the multiple layers of polyelectrolyte / quantum dot thin films. According to the method, a visual biochip is prepared on the substrate material through an ink-jet printer and is applied to organic pesticide residue detection; the multiple layers of polyelectrolyte / quantum dot thin films with different signal intensities can be obtained by controlling the printing times, and the performance of the sensor can be further improved by controlling the assembling concentration of enzyme and the number of protein layers. The preparation method disclosed by the invention is simple, easy to operate, high in repetitiveness, high in precision and low in detection limit; the structure of the sensor can be controlled, and quick site detection can be implemented.

The present invention relates to a particle comprising a non-metallic core having a fluorescent material and a metallic shell encapsulating the non-metallic core wherein the metallic shell has transparency for an electromagnetic radiation having a first wavelength to excite the said fluorescent material and reflectance for an electromagnetic radiation having a second wavelength emitted by the said fluorescent material to confine the electromagnetic radiation having the second wavelength in the metallic shell. This system allows for the excitation of optical cavity modes inside the particle even at sub-micron particle size. The cavity modes are extremely sensitive to any change of the dielectric environment of the particle. This sensitivity can be used for the construction of optical nano-biosensors. Another application of the system is that of a microscopic source for spherical light waves, which may find applications in digital inline holography and display technology.

The invention discloses a mesoporous biomaterial containing porcine pancreatic lipase and a preparation method of the mesoporous biomaterial. The method comprises the steps of dissolving surfactant in water solution of hydrochloric acid, adding inorganic silicon source and organic silylating reagent, fully hydrolyzing under a water bath condition, aging, cooling, filtering, washing, drying and extracting the surfactant to obtain mesoporous silica containing an active group, then putting the prepared material into buffer solution of porcine pancreatic lipase, centrifuging, filtering and drying to obtain the mesoporous biomaterial with functionalized porcine pancreatic lipase. The method disclosed by the invention is simple and environment-friendly. The synthetic mesoporous biomaterial containing the porcine pancreatic lipase has the characteristics of large aperture, high specific surface area, high enzyme activity and large enzyme activity increasing proportion, and can be directly used as biological catalytic material. The mesoporous biomaterial containing the porcine pancreatic lipase disclosed by the invention has wide application prospect in these respects of not only bioreactors, but also nano-bio-sensors, biomolecular delivery and the like.

The invention belongs to the technical field of preparation and application of nano / micrometer scale materials, and particularly relates to a preparation method and application of a two-dimensional nano rare earth borate laccase sensor. The preparation method specifically comprises the steps of firstly, synthesizing a rare earth borate nano material by a solid-state hydrothermal process; and then immobilizing laccase in a lamellar structure of the rare earth borate nano material to prepare the two-dimensional nano rare earth borate laccase sensor. The electrochemical property of the two-dimensional nano rare earth borate laccase sensor in a phenol solution is researched, and the two-dimensional nano rare earth borate laccase sensor is applied to monitoring and processing of phenols pollutants in a water body. The laccase is immobilized by the laminar borate nano material, and the preparation method is simple and efficient; a method for determining hydroquinone and pyrocatechol by the laccase / rare earth borate nano biosensor is high in sensitivity, simple and easy to operate, and high in application potential.

The invention discloses a method for detecting vibrio parahaemolyticus through multiple cross amplification in combination with gold-nano biosensing. According to the method, biotin is marked at 5' end of a cross primer CP1 or CP2 in multiple cross displacement amplification, a hapten is marked at 5' end of an amplification primer C1 or C2, and amplification primers for the toxR gene of the vibrio parahaemolyticus can be visually detected by a gold-nano biosensor. The method is convenient, fast, sensitive, specific and suitable for detecting various nucleotide fragments.

The invention discloses a multiple cross isothermal amplification method implemented by the aid of Antarctic thermal sensitive uracil-DNA (deoxyribonucleic acid)-glycosylase (AUDG) coupled with self-avoiding molecular recognition systems (SAMRS). The multiple cross isothermal amplification method includes carrying out SAMRS modification on four basic groups from a second basic group to a fifth basic group from the bottom of a 3' end of each primer during multiple cross displacement amplification; labeling haptens at 5' ends of amplification primers C1 or C2; leading the AUDG, deoxidized uracil and biotinylated deoxidized cytosine into amplification systems; detecting amplification products by the aid of coupled macromolecule nano biosensing on the basis of multiple cross displacement amplification technologies. The multiple cross isothermal amplification method has the advantages that the amplification products of IS6110 specific sequences of mycobacterium tuberculosis complexes can be pertinently visually detected by macromolecule nano biosensors; the multiple cross isothermal amplification method is convenient, speedy, sensitive and specific and is suitable for detecting diversified nucleotide fragments.

There is provided nanobiosensors and more particularly sensors comprising one or more aptamers or other functional nucleic acids adapted for signalling incorporated within a nanoparticle comprising polyacrylamide or other suitable polymer. Moreover, there is provided a novel DNA aptamer, which selectively binds to ATP. There is also provided a novel nanobiosensor for monitoring ATP concentrations in samples, including biological samples; this new approach may be used to monitor kinase activity in a given sample.

The invention provides a double-signal non-enzyme signal amplification RNA (ribonucleic acid) nano-biosensor, a preparation method and an application of the nano-biosensor. Compared with the prior art, in order to amplify electrochemical response, miRNA concentration is quantitatively measured by taking 'delta IMB+delta IFc' as response signals. Compared with the prior art, the electrochemical sensor is high in sensitivity and selectivity, low in detection limit, simple to operate, small in size, portable and low in price and cost, complicated operation steps and sample pretreatment are omitted, results can be rapidly acquired, the electrode size is small, mass transfer rate is high, and detection is more sensitive.

The invention relates to a polydopamine quantum dot-based fluorescence enhanced detection method of sulfur dioxide derivatives and belongs to the technical field of nano-biosensors. The method comprises synthesizing polydopamine quantum dots (PDADs) emitting blue fluorescence at the room temperature through dopamine (DA) as a sole precursor, and adding sulfur dioxide derivatives into a buffer solution containing PDADs so that PDADs fluorescence is enhanced and the high sensitivity and selective detection of sulfur dioxide derivatives are realized. The fluorescent nanoprobe realizes the detection of sulfur dioxide derivatives in a complex system (comprising human serum and urine) and the fluorescence imaging of intracellular sulfur dioxide derivatives. The polydopamine quantum dot fluorescent probe has the advantages of simple synthesis, low biotoxicity, high sensitivity to sulfur dioxide derivative detection, good chemical stability and biocompatibility.

The invention belongs to the field of medical disinfection, and discloses an ultraviolet disinfecting system for an operating room. The ultraviolet disinfecting system comprises an information acquisition unit, an information processing unit, an implementing unit, a timed starting unit and a power supply unit, wherein the information acquisition unit comprises a nano biosensor and a human body proximity sensor; the information acquisition unit is connected to the information processing unit; the information processing unit comprises a single chip controller which is connected to the information acquisition unit, a display screen which is connected to the single chip controller and a Flash memory; the implementing unit comprises an ultraviolet lamp, a camera, a voice broadcast device and an electromagnetic lock; the implementing unit and the single chip controller are connected; the timed starting unit comprises a 555 timer which is connected to the power supply unit and a touch switch which is connected to the 555 timer; and the power supply unit is electrically connected to the information acquisition unit, the information processing unit and the implementing unit by virtue of the timed starting unit. With the application of the ultraviolet disinfecting system provided by the invention, the environment of the operating room can be monitored in real time and comprehensive and effective disinfection can be automatically conducted, so that the labor intensity of cleaning personnel is reduced.