The invention discloses a mesoporous biomaterial containing porcine pancreatic lipase and a preparation method of the mesoporous biomaterial. The method comprises the steps of dissolving surfactant in water solution of hydrochloric acid, adding inorganic silicon source and organic silylating reagent, fully hydrolyzing under a water bath condition, aging, cooling, filtering, washing, drying and extracting the surfactant to obtain mesoporous silica containing an active group, then putting the prepared material into buffer solution of porcine pancreatic lipase, centrifuging, filtering and drying to obtain the mesoporous biomaterial with functionalized porcine pancreatic lipase. The method disclosed by the invention is simple and environment-friendly. The synthetic mesoporous biomaterial containing the porcine pancreatic lipase has the characteristics of large aperture, high specific surface area, high enzyme activity and large enzyme activity increasing proportion, and can be directly used as biological catalytic material. The mesoporous biomaterial containing the porcine pancreatic lipase disclosed by the invention has wide application prospect in these respects of not only bioreactors, but also nano-bio-sensors, biomolecular delivery and the like.