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Nano biosensor for detecting PML/RAR alpha fusion gene of acute promyelocytic leukemia

A technology of promyelocytes and biosensors, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and electrochemical variables of materials, etc. It can solve the problems of long detection cycle, high price, low sensitivity, etc., and achieve the detection cycle Effects of shortness, improved specificity, and enhanced sensitivity

Inactive Publication Date: 2010-05-12
林新华 +4
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Problems solved by technology

[0006] The technical problem to be solved by the embodiments of the present invention is to provide a nano-biosensor for detecting the PMI / RARα fusion gene of acute promyelocytic leukemia, which aims to solve the problems of low sensitivity, long detection period and high price of existing detection methods.

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  • Nano biosensor for detecting PML/RAR alpha fusion gene of acute promyelocytic leukemia
  • Nano biosensor for detecting PML/RAR alpha fusion gene of acute promyelocytic leukemia
  • Nano biosensor for detecting PML/RAR alpha fusion gene of acute promyelocytic leukemia

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Embodiment Construction

[0018] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0019] In the method for detecting the PML / RARα fusion gene of acute promyelocytic leukemia provided by the embodiments of the present invention, the PML / RARα fusion gene to be detected is L-type, V-type or S-type, comprising the following steps:

[0020] (1) The breakpoint of the PML / RARα fusion gene on chromosome 17 is fixed in the second intron of the RARα gene, while there are three breakpoint cluster regions (BCR) on chromosome 15, Known as bcr1, bcr2 and bcr3, they are respectively located in the sixth intron, sixth exon and third intron of the PML gene. Due to the different positions...

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Abstract

The invention provides an electrochemical DNA biosensor for detecting PML / RAR alpha fusion gene of acute promyelocytic leukemia. The gene to be detected is the PML / RAR alpha fusion gene of the acute promyelocytic leukemia (APL); and the method comprises the following steps: (1) designing and synthesizing a specific sequence of the APL according to the selected fusion sites of a universal primer sequence of the gene type of the APL to be detected; and (2) constructing a nano modified electrode by a Au nano material, and constructing the nano electrochemical biosensor used for detecting the PML / RAR alpha fusion gene of the APL by the electrochemical enzyme-linked immunoassay technology. The method greatly improves the sensitivity of the biosensor so as to realize early diagnosis of the acute promyelocytic leukemia.

Description

technical field [0001] The invention relates to a nano biosensor for detecting PML / RARα fusion gene of acute promyelocytic leukemia. Background technique [0002] Acute promyelocytic leukemia (APL) is M in the FAB classification of acute myeloid leukemia (AML) 3 It is one of the malignant hematological diseases that seriously endanger human health. It has an acute onset, rapid deterioration, very dangerous performance, obvious bleeding tendency, prone to disseminated intravascular coagulation, high mortality rate, and difficult treatment. The level of early leukemia diagnosis (gene diagnosis) will directly affect the treatment effect and quality of life of patients, and its significance is self-evident. [0003] At present, the clinical diagnosis methods of APL mainly include chromosome analysis, Southern-Blot, RT-PCR and FISH and other techniques, but these methods have certain limitations: chromosome analysis is time-consuming and laborious, and has low sensitivity; Sout...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/26
Inventor 林新华刘爱林陈敬华魏娜陈伟
Owner 林新华
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