12 results about "Enzyme linked immunoassay" patented technology

The utility model discloses automatic enzyme-linked immunoassay detection equipment which comprises a processing module and a washing liquid module, and the processing module comprises a pipetting module, a clamping jaw module and an operation processing area, the operation treatment area is provided with a reaction container, a cleaning position, a cleaning head matched with the cleaning position, an oscillation incubation area, a sample adding area, a consumable loading area, a reagent loading area and an enzyme immunoassay detection area, the cleaning head comprises a liquid injection needle and a liquid suction needle, and the pipetting module and the clamping jaw module can be driven to move along the operation treatment area. The pipetting module can transfer a sample liquid or a reagent to the reaction container, the clamping jaw module can move to the reaction container to perform cover opening and closing operation and can transfer the reaction container into a cleaning position, and the washing liquid module can be in fluid communication with the liquid injection needle and the liquid suction needle to complete cleaning of the reaction container. The detection equipment provided by the utility model can realize a series of full-automatic enzyme immunoassay detection such as sample transfer, reagent transfer, enzyme immunoassay reaction, cleaning and enzyme immunoassay detection.

The invention relates to a method for detecting the in vitro relative potency of a type 9 HPV vaccine or antigen stock solution. Specifically, the in vitro relative potency of an HPV-VLP antigen stock solution and vaccine finished products (types 6, 11, 16, 18, 31, 33, 45, 52 and 58) is detected by using an enzyme-linked immunosorbent assay. The enzyme-linked immunosorbent assay has excellent accuracy and precision, the standardization level of HPV vaccine quality evaluation is improved, and a foundation is expected to be laid for promoting research and development of high-quality HPV vaccines.

This invention belongs to the field of analytical detection technology and relates to a method for detecting estradiol using a combination of organometallic framework enrichment and enzyme-linked immunosorbent assay (ELISA). A magnetic organometallic framework solid-phase extractant is used to enrich estradiol in the sample, followed by detection of the enriched estradiol using ELISA. The magnetic organometallic framework solid-phase extractant is prepared by subjecting mercaptoacetic acid-modified Fe3O4 particles, zirconium salt, and 2-amino-terephthalic acid to a solvothermal reaction at 110–130°C for 22–26 hours. The detection method provided by this invention has advantages such as short detection time, high sensitivity, good specificity, and high accuracy, thereby enabling the detection of trace amounts of estradiol in environmental water samples.

The present application relates to the application of enzyme-linked immunoassay for detecting the in vitro relative potency of HPV-VLP antigen stock solution and vaccine finished product (types 6, 11, 16 and 18). The enzyme-linked immunoassay of the present application has universality for detecting antigens produced by different expression systems (Hansenula, Pichia, Escherichia coli and insect cells), and improves the standardization level of HPV vaccine quality evaluation, and lays a foundation for promoting the research and development of high-quality HPV vaccine.

The present application relates to a method for detecting the in vitro relative potency of HPV-9 vaccine or antigen stock solution, and specifically applying enzyme-linked immunoassay to detect the in vitro relative potency of HPV-VLP antigen stock solution and vaccine finished product (types 6, 11, 16, 18, 31, 33, 45, 52 and 58). The enzyme-linked immunoassay of the present application has excellent accuracy and precision, improves the standardization level of HPV vaccine quality evaluation, and is expected to lay a foundation for promoting the research and development of high-quality HPV vaccine.

The invention relates to a hybridoma cell strain secreting a dimethyl yellow monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. 4-(4-aminophenyl) butyric acid and 4-dimethylphenol are used as raw materials to prepare a dimethyl yellow hapten through a diazo method reaction, a dimethyl yellow complete antigen is further obtained, a hybridoma cell strain is obtained through cell fusion and screening of an indirect competitive enzyme-linked immunosorbent assay, the cell strain secretes a dimethyl yellow monoclonal antibody, and the monoclonal antibody can be used for preparing the dimethyl yellow monoclonal antibody. The kit has good detection specificity and detection sensitivity on dimethyl yellow, has an IC50 value of 1ng / mL, can be applied to preparation of an immunodetection product of dimethyl yellow, and provides a brand new efficient tool for detection of dimethyl yellow.

The invention provides a benzophenanthrene polycyclic aromatic hydrocarbon hapten, a monoclonal antibody, a hybridoma cell strain and application, and relates to the technical field of immunodetection. According to the invention, triphenylene hapten is synthesized, triphenylene complete antigen is prepared, cell fusion and indirect competitive enzyme-linked immunosorbent assay screening are carried out, a hybridoma cell strain is obtained, the hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46732, and the hybridoma cell strain can be used for preparing a triphenylene monoclonal antibody. The monoclonal antibody secreted by the hybridoma cell strain has good detection sensitivity to benzo [a] pyrene, benzo [a] anthracene, benzo [b] anthracene, dibenzo [a, h] anthracene and benzo [k] anthracene, the IC50 values are 6.3 ng / mL, 19.4 ng / mL, 6.8 ng / mL, 55.5 ng / mL and 21.7 ng / mL respectively, and the monoclonal antibody can be used for establishing an immunological detection method for benzophenanthrene polycyclic aromatic hydrocarbons.

The application discloses a diabetic nephropathy diagnosis biomarker and application thereof, and belongs to the field of in-vitro diagnosis, wherein the diabetic nephropathy diagnosis biomarker is a combination of 8-hydroxydeoxyguanosine and nitrate; the concentration of 8-hydroxydeoxyguanosine and nitrate in urine can be detected by a double-antibody enzyme-linked immunoassay and a nitric oxide analyzer respectively, and then a diabetic nephropathy diagnosis scheme based on the two biomarkers is constructed; the scheme has the advantages of non-invasiveness and high specificity, can accurately distinguish diabetic nephropathy from diabetes, and provides a new strategy for early screening and diagnosis of diabetic nephropathy.

The invention discloses a method for detecting human APOH based on a double-antibody sandwich ELISA technology, and belongs to the technical field of biology, the method comprises the following steps: preparing a rabbit-source APOH antibody and a mouse-source APOH antibody, coating, washing a plate, sealing, and adding a sample to be detected; and incubating the second antibody, developing, and measuring the OD450 value by using a microplate reader after the color development is ended. The preparation method has the beneficial effects that the APOH recombinant protein is used as an antigen to immunize rats and New Zealand folded-ear rabbits to prepare antiserum, and the antiserum is purified to obtain the polyclonal antibody; the obtained antibody is used for detecting human APOH by using a double-antibody sandwich enzyme-linked immunosorbent assay. The antibody preparation process and the double-antibody sandwich ELISA technology have the advantages of high specificity, stability, reliability, rapidness, low cost and the like.

The application discloses a kind of gold cluster-manganese dioxide nanosheet-based pesticide fluorescence immunoassay method.Currently, it is still challenging to develop a high-sensitivity, strong anti-interference ability analysis method to detect pesticides in complex food matrix.In the present application, coated antigen competes with pesticide to recognize enzyme-labeled antibody.After competitive immune reaction, ALP catalyzes substrate to produce ascorbic acid, which triggers the decomposition of AuNCs-MnO2NFs and regulates fluorescence reaction.Methidathion is selected as the analyte to be detected by the method.The results show that the fluorescence immunoassay method has high detection sensitivity (IC 10 = 5.78 pg mL ‑1 ), which is about 56 times higher than the traditional enzyme-linked immunoassay.The present application is successfully used for precise monitoring of pesticide residues and degradation dynamics in pakchoi, and has good anti-interference ability and reproducibility, paving the way for pesticide residue determination in actual samples.

The invention discloses a construction method of a saccharomyces cerevisiae surface display engineering strain for efficiently expressing aflatoxin oxidase, which is characterized in that an aflatoxin oxidase gene Afo after codon optimization is connected to a saccharomyces cerevisiae expression vector pYD1 through the steps of double enzyme digestion, connection, transformation and the like, and a recombinant display plasmid pYD1-Afo is successfully constructed. A lithium acetate method is adopted to convert the recombinant display plasmid pYD1-Afo into tryptophan auxotroph saccharomyces cerevisiae EBY100, a plurality of recombinant transformants are obtained through MD plate screening, and screening and verification are carried out. And determining the whole-cell enzyme activity of the recombinant strain with the aflatoxin oxidase displayed on the surface by using enzyme-linked immunosorbent assay, and screening out the strain with high enzyme activity. The engineering yeast strain constructed by the method not only can obviously improve the detoxification efficiency, but also can reduce the cost and the pollution to the environment.

The present application relates to a kind of pantothenic acid secreting monoclonal antibody hybridoma cell strain and its application, belong to the field of immunochemistry technology.The present application is prepared by synthesizing pantothenic acid hapten, pantothenic acid complete antigen, after cell fusion and the screening of indirect competition enzyme-linked immunoassay, hybridoma cell strain is obtained, and the pantothenic acid monoclonal antibody secreted by cell strain has good specificity and detection sensitivity to pantothenic acid, IC 50 Value is 25.67ng / mL;With pantothenic acid monoclonal antibody preparation obtained quantitative detection pantothenic acid time resolution fluorescent test strip, by optimizing the amount of fluorescent microspheres of test strip, further improve the detection sensitivity of test strip, the minimum detection limit of obtained test strip to pantothenic acid is 5.08 μg / kg, and the linear detection range is 3.79-40.15 μg / kg.The present application can be used for the rapid quantitative detection of pantothenic acid in food, and solves the problem of poor specificity and detection sensitivity in the current pantothenic acid immunodetection method.