The invention discloses an ochratoxin A fluorescence detection test strip and application thereof, relates to a method for detecting ochratoxin A by using a fluorescence test strip of a quantum dot-labeled aptamer, and belongs to the technical field of fluorescence detection. The test strip comprises a lower water-absorbent pad (1), a quantum dot-coupled Aptamer 1 (2), a streptavidin-biotin-Aptamer 2 (3), a streptavidin-biotin-Aptamer 3 (4), an upper water-absorbent pad (5), a nitrocellulose membrane (6) and a bottom plate (7). By using a chromatography one-step competition principle, the test strip semiquantitatively detects ochratoxin A residue quantity in a semiquantitative detection sample through upper and lower colorimetric belts thereof, rapidly and accurately detects whether the sample contains the ochratoxin A within 15 min to determine whether the ochratoxin A is overproof, can meet the requirement of food safety on the detection of the ochratoxin A residue quantity, and is suitable for feeds, meat producing plants and government detection mechanisms; and compared with the prior art, the test strip has the characteristics of convenient use, economy, rapidness, simple manufacturing and low cost.