6 results about "Ochratoxin A" patented technology

This invention presents a method for fabricating an electrochemical sensor based on a DNA windmill structure for the simultaneous detection of AFB1 and OTA. The aptamers of AFB1 and OTA, along with their complementary double-stranded structures Apt-1 / cDNA-1 and Apt-2 / cDNA-2, are respectively connected to magnetic beads. In the presence of AFB1 and OTA, cDNA-1 detaches into the supernatant, while cDNA-2 remains on the magnetic beads. After magnetic separation, PtCuMn / S1 / AQ / HP1 is added to the supernatant, causing DNA windmill blade probe-1 to detach. Pd@Pt / S2 / Thi / HP2 is then added to the precipitate, causing DNA windmill blade probe-2 to detach. The DNA windmill blade probe is then dropped onto an electrode loaded with a DNA windmill backbone to form a DNA windmill structure. Quantitative detection of AFB1 and OTA is achieved by measuring the signal changes of AQ and Thi.

The invention belongs to the field of biological detection kits, and particularly discloses a chemiluminescence immunoassay kit for detecting mycotoxin. The kit is based on a homogeneous immunoassay principle, and can quantitatively detect aflatoxin B1, vomitoxin, zearalenone, ochratoxin A and citrinin in a sample. The core of the invention is to provide an integrated detection system which comprises special reagent cards for the five toxins and a unified sample pretreatment module (an extracting solution and a diluent). Each reagent card is internally pre-coated with freeze-dried photosensitive microspheres and luminous microspheres coated with specific antibodies or antigens, and automatic identification and traceability of detection information are realized through two-dimensional codes. Through an optimized reagent formula and a preparation process, multi-index, high-throughput, rapid and accurate detection is realized, the problems of tedious operation, low efficiency, incapability of multi-joint detection and the like in the prior art are solved, and the method is suitable for rapid screening and monitoring of grain and oil food safety.

The invention discloses mixed-bacterium fermented rice noodles and a preparation method thereof, belongs to the technical field of food processing, and aims to solve the problems of unstable natural fermentation quality, low production efficiency, single nutrition and more solid wastes of traditional rice noodles. The raised rice noodles are composed of early indica rice grain components, characteristic fermentation metabolites, optimized nutrient components and water. The preparation method comprises the following steps: pretreating rice, grinding into thick liquid, homogenizing, inoculating with mixed bacteria, fermenting, steaming, drying and forming. Through synergistic fermentation of leuconostoc mesenteroides, streptococcus thermophilus and rhodotorula mucilaginosa according to the ratio of 1.5: 1.5: 1 and in combination with strain domestication and a double-stage temperature control process, the effects that the taste and color difference between batches of the rice raising noodles are effectively reduced, the fermentation period is shortened, and the infectious microbe contamination rate is reduced are achieved; the utilization rate of protein and the content of dietary fibers are greatly increased, the removal rate of ochratoxin A is increased by matching with glucose oxidase, and solid waste discharge is reduced by adding back rice residues.

The invention belongs to the field of biological detection kits, and discloses a method for detecting mycotoxin through laser-excited chemiluminescence. The core of the method is that a laser beam with the wavelength of 680 + / -10nm and the power density of 100-500mW / cm < 2 > is adopted to excite the photosensitive microspheres in a detection system in a pulse or continuous scanning mode, and chemiluminescence signals generated after the detection are detected, so that the detection sensitivity of the photosensitive microspheres is improved. The rapid, parallel and quantitative detection of five mycotoxins, namely aflatoxin B1, vomitoxin, zearalenone, ochratoxin A and citrinin, in a single sample is realized. The system includes a specific laser source, a sample compartment, a photodetector, and a processor. The matched kit provides a unified sample pretreatment solution and independent freeze-drying reagent cards of various toxins. The excitation efficiency and the signal-to-noise ratio are improved by optimizing laser parameters, the problems that in the prior art, sensitivity is insufficient, operation is tedious and flux is low are solved in combination with the mode of one liquid and multi-detection parallel, and the method is suitable for large-scale rapid screening of grains and products of the grains.

The invention discloses a colorimetric biosensing system based on phenylalanine dehydrogenation, which is used for detecting ochratoxin A. The invention provides a method for detecting OTA in a to-be-detected sample, which comprises the following steps: A1) carrying out hydrolysis reaction on the to-be-detected sample by using amide hydrolase VeADH to obtain a first-step reaction solution; a2) adding phenylalanine dehydrogenase, coenzyme NAD < + > and Glycine into the first-step reaction solution, and reacting again to obtain a second-step reaction solution; adding a color developing agent into the second-step reaction solution, and carrying out color developing reaction to obtain a reaction product; detecting OTA in the sample to be detected according to the reaction product; according to the invention, a novel OTA degrading enzyme VeADH of an OTA sensing element and a product L-phenylalanine sensing element phenylalanine dehydrogenase are combined, and efficient, visual and quantitative detection of OTA is realized by using a color reaction in a product catalysis process.