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Method for preparing visual biosensor for detecting organophosphorus pesticides based on ink-jet printing technology

An organophosphorus pesticide and biosensor technology, applied in the field of nano biosensors, can solve the problems of complicated assembly steps, narrow application range, easy inactivation of enzymes, etc., and achieve the effects of controllable sensor structure, high sensitivity and easy operation.

Inactive Publication Date: 2014-02-05
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method now faces shortcomings such as low accuracy, narrow application range, cumbersome assembly steps, and easy inactivation of enzymes.

Method used

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  • Method for preparing visual biosensor for detecting organophosphorus pesticides based on ink-jet printing technology
  • Method for preparing visual biosensor for detecting organophosphorus pesticides based on ink-jet printing technology
  • Method for preparing visual biosensor for detecting organophosphorus pesticides based on ink-jet printing technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Gradient dilution of cationic polyelectrolyte solution with deionized water, final mass concentration of 0.007~0.35%, filtration with 0.22 μm filter membrane;

[0019] (2) The newly prepared quantum dot solution is concentrated by centrifugation to remove excess stabilizers and ions in the solution;

[0020] (3) Refit an ordinary office printer into an ink supply system, design different patterns through the corresponding software of the printer, and inject cationic polyelectrolyte solution and quantum dot solution into the cartridge;

[0021] (4) Alternately print and deposit polyelectrolyte and quantum dot solutions on the base material, prepare 10-100 double-layer polyelectrolyte / quantum dot multilayer films, dry at room temperature, and be tested;

[0022] (5) Then, add polyelectrolyte and protein solution alternately on the multilayer film prepared in step (4) to prepare 0-5 double-layer (polyelectrolyte / protein) multilayer films, and finally continue to alternately add...

Embodiment 2

[0028] The difference between this embodiment and embodiment 1 is that in step (5), polyelectrolyte and acetylcholinesterase solution (0.0025~0.5 mg / mL) are alternately added dropwise on the multilayer film prepared in step (4) to prepare 1~ 5 double-layer (polyelectrolyte / acetylcholinesterase) multilayer films.

Embodiment 3

[0030] The polydiallyldimethylammonium hydrochloride (PDDA) solution was diluted with deionized water to a concentration of 0.07%, and then filtered with a 0.22 μm filter membrane; ultrafiltration concentrated the newly prepared CdTe quantum dot solution (the molecular weight cut-off of the ultrafiltration tube was 30 kDa), remove the excess stabilizer and ions in the solution, and the final concentration is 5x10 -4 mol / L quantum dot solution; add the above two solutions to a common office inkjet printer, and then alternately print 25 (PDDA / CdTe) double layers on a filter paper substrate treated with acetone, and dry at room temperature (as required Different patterns can be printed, dot matrix in this example); Then, add 0.07% PDDA and 0.5mg / ml bovine serum albumin (BSA) alternately on the printed array, and deposit 2 (PDDA / BSA) double layers ; Finally, continue to drop 0.07% of PDDA and 0.25mg / ml acetylcholinesterase (AChE), continue to deposit 2 (PDDA / AChE) double layers...

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Abstract

The invention discloses a method for preparing a visual biosensor for detecting organophosphorus pesticides based on an ink-jet printing technology and belongs to the technical field of nano biosensors. The method comprises the steps of preparing a quantum dot solution with negative charge and a polyelectrolyte solution with positive charge; alternately printing the quantum dot solution and the polyelectrolyte solution onto a substrate material to obtain multiple layers of polyelectrolyte / quantum dot thin films; alternately dropping polyelectrolyte and acetylcholin esterase onto the multiple layers of polyelectrolyte / quantum dot thin films. According to the method, a visual biochip is prepared on the substrate material through an ink-jet printer and is applied to organic pesticide residue detection; the multiple layers of polyelectrolyte / quantum dot thin films with different signal intensities can be obtained by controlling the printing times, and the performance of the sensor can be further improved by controlling the assembling concentration of enzyme and the number of protein layers. The preparation method disclosed by the invention is simple, easy to operate, high in repetitiveness, high in precision and low in detection limit; the structure of the sensor can be controlled, and quick site detection can be implemented.

Description

technical field [0001] The invention belongs to the technical field of nano-biosensors, and relates to a method for preparing a visual biosensor for organophosphorus pesticide detection based on inkjet printing technology. Background technique [0002] Organophosphorus pesticides are widely used in global agricultural production as an effective method to kill pests in crop growth. While ensuring crop yields, they also bring a lot of trouble to humans. Therefore, the detection of pesticide residues in crops is very important. Among the current detection methods, gas chromatography-mass spectrometry is the most widely used technology, with accurate results and high sensitivity, but the equipment is large in size, requires professional operation, cumbersome sample pretreatment, long detection time, inability to conduct on-site detection and a large number of Sample screening. Similarly, some other sensors, such as electrochemical sensors and plasma sensors, also have shortcom...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 刘绍琴果崇申颜美郑兆柱
Owner HARBIN INST OF TECH
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