Mesoporous biomaterial containing porcine pancreatic lipase and preparation method of mesoporous biomaterial
A technology of biological materials and mesoporous materials, which is applied in the same direction fixed on or in an inorganic carrier to achieve the effect of large pore size, large specific surface area and environmental friendliness
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Embodiment 1
[0025] Dissolve 2 grams of non-ionic surfactant P123 in 15 grams of water and 60 grams of 2M hydrochloric acid solution, stir at room temperature for 30 minutes until clear, place it in a water bath at 40°C, add tetraethyl orthosilicate 3.83 grams, after pre-hydrolysis for 2 hours, add 0.49 grams of chloropropyl-triethoxysilylating reagent, stir at 40°C for 24 hours, then transfer to a reaction kettle with a polytetrafluoroethylene liner, and place it at 100°C In an oven, aged for 24 hours, filtered, washed, and dried naturally in the air, and finally extracted in ethanol hydrochloric acid solution for 24 hours to remove the surfactant, dried at room temperature, and the proportion of chlorine groups in the total silicon was 10. % mesoporous silica (SBA-Cl-10%). The above materials were added to 20ml of porcine pancreatic lipase buffer solution with a concentration of 2mg / mL, and the mixture was mixed at 25°C and 215rmp for 3 hours. Then, place it at 10° C. and centrifuge at ...
Embodiment 2
[0027] Dissolve 4 grams of nonionic surfactant P123 in 125 grams of 1M hydrochloric acid solution, stir at room temperature for 35 minutes until clear, place it in a water bath at 35°C, add 4.04 grams of tetraethyl orthosilicate, and pre-hydrolyze 1.5 Hours later, add 0.25 g of mercaptopropyl-triethoxysilylating reagent, stir at 35°C for 24 hours, then transfer to a reaction kettle with a polytetrafluoroethylene liner, put it in a 100°C oven, and age for 24 hours. Hours, filtered, washed, dried naturally in the air, and finally extracted in ethanol hydrochloric acid solution for 24 hours to remove the surfactant, dried at room temperature to obtain a mesoporous silica containing mercapto groups in the total silicon with a ratio of 5%. (SBA-SH-5%). The above materials were added to 20ml of porcine pancreatic lipase buffer solution with a concentration of 2mg / mL, and the mixture was mixed for 1 hour at 25°C and 215rmp. Then, place it at 10° C. and centrifuge at 6000 rpm for 10 ...
Embodiment 3
[0029] Dissolve 1 gram of non-ionic surfactant P123 in 7.5 grams of water and 30 grams of 4M hydrochloric acid solution, stir at room temperature for 40 minutes until clear, place it in a water bath at 45°C, add 1.4 methyl orthosilicate grams, after pre-hydrolysis for 1 hour, add 0.20 grams of vinyl-triethoxy silylating reagent, stir at 45°C for 24 hours, then transfer to a reaction kettle with a Teflon liner, and put it into a 100°C oven , aged for 24 hours, filtered, washed, and dried naturally in the air, and finally extracted in ethanol hydrochloric acid solution for 24 hours to remove the surfactant, dried at room temperature, and the proportion of vinyl groups in the total silicon was 10%. Mesoporous silica (SBA-YX-10%). The above materials were added to 20ml of porcine pancreatic lipase buffer solution with a concentration of 2mg / mL, and the mixture was mixed at 25°C and 215rmp for 9 hours. Then, place it at 10° C. and centrifuge at 6000 rpm for 10 minutes, filter off ...
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