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Nano biological sensor for detecting NADH concentration and its detecting method

A biosensor and sensor technology, applied in biochemical equipment and methods, microbiological determination/inspection, color/spectral characteristic measurement, etc., can solve the problem of expensive equipment cost operators, inability to perform online detection, and unsatisfactory optical effect detection methods And other issues

Inactive Publication Date: 2006-08-23
HUNAN UNIV
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Problems solved by technology

Therefore, detection methods based on direct measurement of their optical effects are not ideal
Although the method for detecting NADH by high performance liquid chromatography (HPLC) is effective, it requires expensive equipment and professional operators, and cannot be detected online

Method used

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  • Nano biological sensor for detecting NADH concentration and its detecting method
  • Nano biological sensor for detecting NADH concentration and its detecting method
  • Nano biological sensor for detecting NADH concentration and its detecting method

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Embodiment Construction

[0035] 1. Pretreatment of slides

[0036] Ordinary glass slides were processed into a length of 30 mm, a width of 9 mm, and a thickness of 1 mm, and were prepared in Pirahna solution (H 2 SO 4 , 98% and H 2 o 2 , 30%, the volume ratio is 4:1) at 60 ° C for 20 min. After washing, in H 2 o 2 , NH 3 and H 2 Soak in a mixed solution of O (volume ratio 1:1:2) at 70°C for 20 min. Rinse with a large amount of water and methanol (chromatographically pure) to remove physical adsorption, and store in methanol at 4°C for later use.

[0037] 2. Preparation of nanosensors

[0038] Prepare a mixed solution of APTMS and methanol (volume ratio 1:1.5), soak the slides in it for 18 hours at 35°C, rinse with methanol to remove weak adsorption, and then dry at room temperature. The diameter is 10nm, the concentration is 1×10 -4 M. AuNP with a drop volume of 75 μL was applied to one side of the glass slide for fixation, left to dry at room temperature for 2 hours, rinsed with water, and...

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Abstract

This invention relates to a kind of nm bio-sensor and its NADH concentration test method, which takes Au nm particles fixed on the surface of a sensor as the crystal seeds, utilizes the principle of Au / Ag nucleocapsid nm particle catalyst increase under the NADH recovery action and an absorption spectrum variance rule to compute the change of nm particle absorption value to test the NADH concentration.

Description

technical field [0001] The invention relates to the detection of coenzymes, in particular to a nano biosensor and a method for detecting NADH concentration thereof. Background technique [0002] Reduced nicotinamide adenine dinucleotide (NADH) widely exists in the living cells of animals, plants and microorganisms, and is an important coenzyme in the process of biological metabolism. In hundreds of metabolic reactions in living cells, NADH is an electron donor, and NAD+ is an electron acceptor with redox activity. They play an electron transfer role in processes such as glycolysis, citric acid cycle, and photosynthesis. important role. In organisms, NADH is involved in the process of vitamin derivation, the production process of adenosine triphosphate (ATP), an extremely important potential substance in organisms, and is the coenzyme of more than 250 dehydrogenases that have been identified, maintaining cell growth, differentiation and Energy Metabolism. In the process of...

Claims

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Application Information

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IPC IPC(8): G01N21/31C12Q1/68
Inventor 汤琳曾光明沈国励牛承岗袁兴中
Owner HUNAN UNIV
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